JPS582737A - Ion concentration analysing apparatus - Google Patents

Abstract

PURPOSE:To use a titled apparatus in a maintenance-free state for a long period, by sending a sample solution, a standard solution, and a regenerating liquid of an ion selective electrode to a cell room selectively. CONSTITUTION:Only standard solutions 6 and 6' are sent to a cell room 1 by actuating a liquid sending pump 10 and operating sending liquid switching means 8 and 8' and an ion selective electrode 2 is calibrated. Next, only a sample solution 5 is introduced into the room 1 by switching the means 8 and the ion concentration is measured. Next, only a regenerating liquid 7 is introduced into the room 1 by switching the means 8 and 8' and is allowed to stand in the room for a prescribed time and then, regenerating treatment of the electrode 2 is carried out. This liquid 7 is a plasticizer of the same kind as that for constituting an ion selective film of the electrode 2.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention is an ion/concentration analyzer using an ion-selective electrode, more specifically, an ion/concentration analyzer that uses an ion-selective electrode and is equipped with an ion-selective electrode O regeneration means, which allows for one-time ion analysis that can be used for a long period of time without maintenance. Regarding equipment.

In the fields of biochemistry and medicine, a target chemical substance is selected from among the many O chemicals present in body fluids, and the soat-
1 - Concubinage is often practiced. For example, there is a system that is useful for diagnosing diseases by knowing the electrolyte components such as sodium, potassium, calcium, or chlorine that are present in a patient's blood, and the O concentration in fake blood. As modern medicine is rapidly progressing, it is becoming increasingly difficult to analyze the chemical substances present in serum.
It became necessary to analyze 0 chemical substances. Conventional serum O
For measuring chemical substances.

Chemical analysis methods such as flame light f method, fluorescence @ light method, etc. have been used. However, recently, we have been able to respond immediately and operate the center during emergency examinations of patients! There is a demand for an analyzer that is easy to operate, and among these, automatic chemical analyzers for clinical tests have been put into practical use that use ion-selective electrodes that can meet these requirements for electrolyte component analysis.

In this case, the detection end of the ion-selective electrode for specific ions in the sample ll1IE is constituted by an ion-selective membrane. When the electrode is immersed in a test solution, this ion-selective membrane acts selectively only on specific ions to be analyzed that are present in the sample St), but does not act on other components. It is a soft and flexible polymer membrane. Such a polymer membrane has a hydrophobicity of 0 corresponding to the electrolyte component to be analyzed.
= Neat Tsurukiyaria (for example, if the target is Na"0, the target is Nensin, if the target is Na"0, palinomycin, etc.) and dioctyl adipate.

It is constructed by adding a plasticizer such as dioctyl phthalate to a plastic material such as polyvinyl chloride or polycarbonate in a fixed amount and then forming a film.

However, from X& to conventional ion concentration analyzers,
When blood serum can be used as a sample, the polymer membrane type ion-selective electrode O ion-selective membrane O lubricant is eluted by the blood serum 11 and diffused into the cleaning solution and standard solution. Do nine.

As the plasticizer content in the ion-selective membrane decreases, the activity of the neat full carrier as an ion-selective substance decreases. That is, there was a drawback that the deterioration of the ion-selective electrode was accelerated and its life span was shortened. In conventional ion concentration analyzers, operations such as replacing the expensive ion selective electrode and replacing the ion selective electrode 110 with the ion selective electrode must be performed frequently.

The authors of this study conducted extensive research to solve the above-mentioned drawbacks of the ion concentration 1ILll determination device by incorporating a polymer membrane type ion-selective electrode. It was discovered that when the film is exposed to the same type of plasticizer as the plasticizer that is the component of the chorion for a predetermined period of time, the iron film regains its #A properties. K11 cane that fires once.

An object of the present invention is to provide an ion concentration analyzer that is equipped with a polymer membrane type ion selection and a 61% active electrode and can be used for a long period of time without maintenance.

In this device, an ion-selective electrode with an ion-selective membrane containing a polymer membrane containing a nate full carrier and a plasticizer, and a comparative electrode IIO are placed in the sample SI and the standard. **In an ion concentration analyzer comprising a liquid feeding means for feeding liquid; and a rounded signal processing means for processing an electric output signal from a group of wrinkled electrodes, the scissors liquid feeding means includes the ion-selective electrode. (Means for permeating liquid through the R1 insect moat; and means for selectively sending the armpit sample-housing, sill standard S*, and wrinkle regenerating liquid to the #cell child. It is characterized by

In the following, the present li@ device will be explained in more detail based on a ninth embodiment shown in FIG. FIG. 1 is a schematic diagram of one embodiment of the present invention.

111, &%A, l is the cell depth, and the ion selective electrode 2 and the comparison electrode 3 are arranged in the cell depth.

All of these are stored in 70-cell 4, and the sample solution,
The standard solution and regeneration solution pass through *h111 from left to right in the figure. 5-is sample 11ml 1.6°6' is the standard solution, and 7 is the regenerated solution. The regeneration liquid 7 is.

The main component is a plasticizer that constitutes the ion-selective membrane of the ion-selective electrode 2. 8 is sample S solution 5 and standard 111118
'0 analogy dl! Sampling piping with liquid feeding switching means controlled from the side cock valve! , I'.

9'' to each solution. 8' is connected to the regenerating liquid 7 via a sampling pipe 9'', and is connected to a transmission switching means such as a three-way cock valve. In the diagram,
4) Any means may be used as long as the transmitter switching devices 12s and 8' can be placed separately and the cell IK can be selectively introduced without causing any merging of the species SNI. .

Set these O liquid feeding switching means appropriately.

The liquid feed pump W is turned off, a predetermined amount of liquid is introduced into the cell, and after the amount of liquid is determined, it is discharged.

Reference numeral 11 denotes a potential difference output O intensifier generated between the ion-selective electrode 2 and the comparison voltage 113011, 12 an arithmetic unit for converting the output into an ion concentration, and an ion 1lIiWIO indicator, all of which constitute a signal processing means.

Using the apparatus of the present invention as described above, an analysis test of the concentration of ox''o ions in commercially available control was conducted.

All of the piping of the apparatus was made of pipes, and the length between the liquid feeding switching means 8.I' and 70-cell 40 was made as short as possible.

You can use liquid transfer pump W ladder 4 pump. In addition.

As this pump, a reciprocating pump or a syringe pump equipped with a check valve can also be used.

The polymeric ion-selective electrode 2 is made of polyvinyl chloride as a base material, diotatyl adipate as a plasticizer, and palinomycin as an ion-selective substance, which are dissolved and heated in 7-run Kml to form a large sheet. A potassium ion selective membrane electrode with a molded potassium ion selective membrane was used and the reference electrode was a silver-silver chloride electrode.

The standard 11116 was a 10-'M[CJlli[, 榔阜霞諌6'was a l G-''M or E) solution, and the regenerating solution 7 was diotatyl adipate.

First, the liquid feeding pump W is operated, and the liquid feeding switching means 8.8'
to add standard solutions 6 and 6'0 to cell l [
Calibrate the electrode by injecting lK to obtain the Nernst response O nod of electrode 2. Next, switch the switching means 8, introduce the fist of the sample solution 5 into the Ryurudo IK, and measure the potential of the electrode 2. The calibration operation was performed before 11 hours and after 5 hours.

Before the end of the measurement, the switching means 8.8' is switched once to introduce only the regenerating I[7 into the cell*IK and leaving it present in the room KW for the electrode 20 regenerating process 9. Then, add the regenerating solution.

With the above operating mode, I operated the book '11 @ Mesm for 1 day and it worked fine for 90 days, calibration operation 211/day, regeneration processing operation 1
times/day.

Nernst slope of electrode output (t11 force sensation [jho: mV)
() Indicate the change in terms of the number of days the equipment has been in operation. Comparison rounding: O result for conventional equipment! & is also indicated by a circle. In the figure, a is the conventional device, and b is the original device.

As can be seen from the table, the superiority of the single real @ device is obvious. In other words, with the conventional ion 811 analyzer, analysis can be completed in about 30 days! Initial fiOmVO output sensitivity: After 50 days, the E started to decrease -48 mV, whereas the 116 ion concentration with polymer membrane type ion-selective electricity @ 0 regeneration mechanism was achieved. The analytical scissors holder is in operation for 9 days.
Even on day 0, the output sensitivity was -315 mV) The decrease in output sensitivity was extremely slight, and there was no problem with serum analysis KPi et al.

As described above, in the device K of the present invention, when analyzing the ion concentration of a sample solution, the plasticizer-1Ih is removed from the ion-selective membrane.
Even if it is lost due to diffusion into the iI casing, it can be replenished by periodically supplying regenerating liquid, so the output sensitivity of the electrode can be maintained over a long period of time.

11. The device of the present invention is capable of replacing the ion-selective membrane of the ion-selective electrode during the replacement of the conventional ion-selective electrode. It is useful for long-term maintenance-free use without frequent maintenance.

[Brief explanation of the drawing]

1Ii1 Figure is a schematic diagram of one actual @device 0-Example ■, 1
12111 is conventionally 0 ion concentration IfiI & place and book -
Il!'s ion am analyzer! The circle ``■'' shows the change in the ion-selective electrode O output sensitivity with respect to the number of days the device was in operation at 9:00 a.m. after analyzing 1 snow.

Claims (1)

[Scope of Claims] 1. = Ion-selective electrode having an ion-selective layer of a polymer layer containing an ethral carrier and a lubricant; Cell IIK sample *ii and standard 1) [Ion concentration analyzer K, which also consists of a round liquid sending means for sending the liquid, and a round signal sending means for processing the electrical output signal from the wrinkled electrode group; Stay. The liquid feeding means K. Recruitment of means for feeding the regenerating solution of Wrinkle Ion Selective Electron Ii
An ion concentration analyzer having a structure including a liquid feeding switching means for selectively feeding the sample reservoir i[, the wrinkle standard solution and the regenerated wave to the wrinkle standard solution, respectively. 'LIII! The O ion am analysis device described in Patent Claim OSS Iris Paragraph 1, in which the reproduction group is characterized by the same type of O obfuscating agent as in the armpit sketch group.