JPS58193686A - Testing device for identification of microorganism - Google Patents

Abstract

PURPOSE:To carry out the collection, suspension, dilution and pipetting of microorganism, continuously, by using a suspension preparation chamber having variable inner volume to enable the transfer of the prepared microbial suspension through a connecting channel to a plurality of medium chambers. CONSTITUTION:The main body of the testing device is furnished with a medium chamber 2 containing a plurality of divided medium chambers, and a suspension preparation chamber 1 which stores distilled water and dilutes the collected microorganisms to a definite concentration. The inner volume of the preparation chamber 1 is made variable and the preparation chamber is connected with the medium chamber 2 through the connecting channel 8. The colony of the microorganisms obtained by pure culture is collected with a collection needle 4, and put into the suspension preparation chamber 1 to obtain a suspension of microorganisms. The suspension is poured dividedly into the plural medium chambers 2 through the connecting channel 8 by pushing down the inner cylinder 5 having the plural medium chambers 2. The microorganisms are cultured in the medium contained in each medium chamber 2, and the species of the microorganisms can be identified from the culture results.

Description

DETAILED DESCRIPTION OF THE INVENTION (1) Background Technical Field of the Invention The present invention relates to an improved testing instrument used in the identification of microorganisms.

When identifying microorganisms, it is necessary to precisely examine the biochemical properties of the microorganisms, which involves culturing the microorganisms in various media with different compositions and observing their growth status and changes in the culture medium. It is done by The present invention relates to a test instrument used when investigating the biochemical properties of such microorganisms.

In order to investigate the biochemical properties of prior art microorganisms, microorganism colonies that have been pure cultured in isolation or enrichment media are scraped off with platinum loops (fishing bacteria), and directly inoculated into a liquid culture medium, or the collected bacteria and moss are collected. After diluting the suspension in sterile water, physiological saline, etc. at an appropriate concentration, the suspension is dispensed into various dry media with different compositions and cultured. This is done by observing things such as However, such a series of operations requires a large number of instruments and time. That is, in the case of the former, containers such as culture media and test tubes must be prepared according to the inspection items, and when inoculating these with microorganisms, the stopper must be removed from each culture medium container. Also, in the case of the latter, Bibe.

The bacterial suspension must be dispensed into various dry media with different compositions using a pipette, dropper, etc.

Furthermore, when comparing the effects of various reagents on microorganisms, it is necessary to take out several culture test tubes several times and compare them while checking the shaking, turbidity, and color tone, which is tedious and time-consuming. There are also administrative hassles such as having to write symbols etc. on each test tube to prevent mistakes.

To solve this inconvenience, a test container having a plurality of successive compartments has been devised (Japanese Patent Laid-Open No. 115985/1983). In this test container, multiple culture chambers with different culture media are provided in one container, so it is convenient for removing and removing the stopper and for comparative observation of the culture medium. There remains the inconvenience of having to dispense it into the culture room.

■0 Purpose of the Invention The present invention involves collecting pure cultured microorganisms, suspending them in sterile water, dispensing the bacterial suspension into media with mutually different compositions, and comparatively observing the bacteria and the culture medium after culturing. An object of the present invention is to provide a test device for identifying microorganisms that can perform a series of operations using one test device.

The above objects are achieved by the microorganism identification test instrument of the present invention described in the following sections.

(1) It has a suspension preparation chamber whose internal volume can be changed, a plurality of culture medium chambers storing mutually different mediums, and a communication hole forming a liquid flow path from the suspension preparation chamber to the medium chamber. A test instrument for identifying microorganisms, comprising a main body and a sampling needle that allows the main body to be exposed to the outside and stored in the suspension preparation chamber.

(2) In the above item 1, the partition wall that partitions the suspension preparation chamber and the culture medium chamber of the main body is configured to be movable in a liquid-tight manner, so that the internal volume of the suspension preparation chamber can be changed. Test instrument for identifying microorganisms as described.

(3) At least a part of the main body constituting the outer wall of the suspension preparation chamber is made of a deformable material, and the inner volume of the suspension preparation chamber can be changed by pressing. Test equipment for identifying microorganisms as described in Section 1.

(4) A suspension preparation chamber is formed at one end of the main body, and a plurality of mutually partitioned culture medium chambers are formed on the other end of a partition wall that partitions the suspension preparation chamber. The test instrument for identifying microorganisms according to any one of the above items 1 to 3, which is provided in contact with the partition wall.

(5) The test instrument for identifying microorganisms according to any one of the above items 1 to 4, in which the communication hole can be opened and closed.

(6) The test instrument for identifying microorganisms according to any one of Items 1 to 5 above, wherein a plurality of communication holes are provided to communicate each medium chamber and the suspension preparation chamber.

(7) A pusher is movably provided in the main body,
The test instrument for identifying microorganisms according to any one of the above items 1 to 6, wherein the tip of the pusher is a collection needle.

(8) The main body includes a cylindrical body having a closed end face at one end and an opening that can be opened and closed on the end face, and a partition wall in which a cylindrical part with both ends open is connected to the inside of the cylindrical body. is arranged so as to be able to slide on the inner wall of the cylindrical body in a fluid-tight manner, and a suspension preparation chamber whose internal volume can be changed on the mouth side of the cylindrical body by the partition wall and the inner surface of the cylindrical body; A culture medium chamber is provided on the end surface of the partition wall not facing the opening, and a pusher with a collection needle at the tip is provided inside the cylindrical portion.
Item 2, 4, 5, or 7 above, wherein the inner wall of the cylindrical part is arranged so as to be able to slide in a liquid-tight manner, and the collection needle is configured to be exposed from the mouth part. A test instrument for identifying microorganisms as described in .

(9) The main body includes a cylindrical body having a closed end surface at one end, an opening that can be opened and closed on the end surface, and a side surface near the end surface made of a deformable material; A partition wall connecting a cylindrical portion with both ends open from the side surface near the end face to the open end side is provided in the shaped body, and the internal volume changes toward the mouth side of the cylindrical body between the partition wall and the inner surface of the cylindrical body. A culture medium chamber is provided on the side of the partition wall not facing the mouth, and a pusher whose tip is a collection needle is placed inside the cylindrical portion. The inner wall of the container is arranged so that it can slide in a liquid-tight manner,
The microorganism identification test instrument according to any one of the above items 3 to 7, which is configured such that the collection needle can be exposed from the mouth.

(10) The test for identifying microorganisms according to the above item 8 or 9, wherein the cylindrical part is provided with a communication hole that communicates with the culture medium chamber, and the communication hole is opened and closed by the vertical movement of the pusher. utensils.

aυ A fitted part that fits the cylindrical part is provided, a communication hole is provided in each of the fitted part and the cylindrical part, and the communication hole is opened and closed by rotating the fitted part or the cylindrical part. (6) A rotating part having a culture medium rotatable in a circumferential direction is provided in the cylindrical part on the partition wall, and the rotating part and the partition wall are connected to each other. The test instrument for identifying microorganisms according to the above item 8 or 9, wherein the communication holes are respectively provided and the communication holes can be communicated with each other by rotating the rotating portion.

03. The test instrument for identifying microorganisms according to any one of the above items 8 to 12, which is configured to slide by pushing down or pushing up the pusher.

0◆ The test instrument for identifying microorganisms according to any one of the above items 8 to 12, wherein the pusher is fixed by a spring, and the pusher is pressed down by pressing the spring.

M Items 8 to 14 above, in which a removable lid or plug is provided on the mouth, and the mouth is opened and closed by attaching and detaching the lid or plug.
A testing device for identifying microorganisms as described in any of the above.

α→ The mouth part is covered with an elastic membrane, and the elastic membrane is pierced from inside with a collection needle at the tip of a pusher to open the mouth part, according to any one of the above items 8 to 15. Test equipment for identifying microorganisms.

At least a part of the main body is substantially transparent, and the inside of the culture medium chamber can be observed from the outside.
The test instrument for identifying microorganisms according to any of Item 6.

0 barrels The test instrument for identifying microorganisms according to any one of the above items 1 to 17, wherein the medium is a dry medium.

19. The test instrument for identifying microorganisms according to any one of the above items 1 to 18, wherein a stirring ball is accommodated in the suspension preparation chamber.

(1) The test instrument for identifying microorganisms according to any one of the above items 8 to 19, wherein the pusher is provided with a stirring plate.

By using the test instrument of the present invention having the above-described structure, a suspension of microorganisms can be dispensed into multiple culture media at once without the need for sterilization or opening operations, and further,
Since suspension dilution, dispensing, and culturing of microorganisms can be carried out continuously in one test instrument, each operation can be carried out quickly and accurately without the risk of contamination with contaminants, making it efficient.

(9) Detailed Description of the Invention The structure of the present invention will be described in detail below based on embodiments shown in the drawings.

FIG. 1 shows an embodiment of a test instrument for identifying microorganisms. This testing device for identifying microorganisms consists of a main body 3 having a suspension preparation chamber 1 and a culture medium chamber 2 which are in communication with each other, and a sampling needle 4 that is retractably attached to the main body 3.

The suspension preparation chamber 1 of the main body 3 is a chamber for suspending and diluting collected microorganisms to an appropriate concentration with sterile water, for example distilled water, and is sealed in advance with sterilized water. This suspension preparation chamber 1 has a structure that allows its internal volume to be changed.

For example, in the case shown in FIG. 1, since the test instrument main body 3 is constructed of a double cylinder consisting of an inner cylinder 5 and an outer cylinder 6, there is a gap between the inner circumferential surface of the outer cylinder 6 and the outer surface of the inner cylinder 5. The partitioned space becomes the suspension preparation chamber 1. The volume of this suspension preparation chamber 1 is changed by pushing the inner cylinder 5 like a syringe, and the suspension 7 which is the content thereof is formed in the inner cylinder 5. It flows backward through the communication hole 8 and flows into the culture medium chamber 2 in the inner cylinder 5. Here, it is necessary that the inner cylinder 5 be maintained in a fitted state such that it can move in a fluid-tight manner with respect to the outer cylinder 6. For example, although not shown, one method is to interpose an O-ring between the inner cylinder 5 and the outer cylinder 6.

Further, as shown in Fig. 5.7.8, the suspension preparation chamber 1 may be formed of a deformable material or structure. For example, in order to make the internal volume variable, it is formed into a bellows shape that can be deformed in the axial direction using a soft synthetic resin or the like. When the suspension preparation chamber 1 is formed of synthetic resin, it can be molded integrally with the outer cylinder 6 that is a part of the main body, or it can be bonded to the outer cylinder 6 that is molded separately. It is possible.

The suspension preparation chamber 1 has an opening 9 through which the sampling needle 4 enters and exits. A cap 10 is attached to the opening 9 in order to keep the sampling needle 4 in a sterile atmosphere and to prevent leakage of the distilled water 7 in the suspension preparation chamber 1. This cap 10 is removed to expose the sampling needle 4 (when collecting microorganisms).Also, before using this test instrument, the C cap may be accidentally removed while the sampling needle 4 is retracted into the suspension preparation chamber 1. In order to prevent the distilled water 7 in the suspension preparation chamber l from leaking when the opening 10 is removed, a rubber membrane 11 may be formed on the opening 9 as shown in FIG.

The medium chamber 2 communicating with the suspension preparation chamber 1 is divided into a plurality of chambers to accommodate a plurality of mediums 12 having different compositions. For example, as shown in FIG. 2, there is a circular culture medium chamber 2a in which the inner cylinder 5 is partitioned radially, or as shown in FIG. 3, there is a rectangular culture medium chamber 2b in which the inner cylinder 5 having a rectangular cross section is partitioned into strips. In the case of the circular culture medium chamber 2a, although the number of culture medium chambers 2 is limited, the device main body 3 can be made smaller;
It has the advantage of being easy to handle. On the other hand, square culture medium chamber 2
In case b, there is an advantage that the number of culture medium chambers 2 can be formed as required. The culture medium chamber 2 contains sterilized dry culture media 12 that are suitable for culturing the microorganisms to be tested and contain substrates suitable for the purpose of the test. Here, as the medium 12, a dry medium is used because it is convenient to handle, but a liquid medium may also be used.

The medium to be used should be determined appropriately depending on the target bacteria or the purpose of the test.

The culture medium chamber 2 and the suspension preparation chamber 1 are communicated with each other through a communication hole 8, and are provided so that the suspension liquid 7 can flow from the suspension preparation chamber 1 whose internal volume can be changed into the culture medium chamber 2. There is.

For example, as shown in FIG. 1, the communication hole 8 is bored in the peripheral wall of a cylindrical portion 13 formed inside the inner cylinder 5 constituting the instrument body 3. Since the communication hole 8 opens above the culture medium chamber 2, it becomes possible to use a powdered culture medium. Further, as shown in FIG. 6-7, a partition wall 14 is formed in the outer cylinder 6 to partition the suspension preparation chamber 1 of the outer cylinder 6, and the partition wall 14 and the bottom surface of the inner cylinder 5 that abuts the partition wall 14 are respectively formed. It is also possible to drill the communication hole 8 so that when the inner cylinder 5 is rotated, both the communication holes 8 coincide and the suspension 7 enters. In this case, the communication hole 8 will open at the bottom of the culture medium chamber 2, so it is not suitable for use with powdered culture medium.
There is an advantage that most of the suspension 7 can be dispensed into the culture medium chamber 2. Further, as shown in FIG. 8, the partition wall 14 is extended to form a cylindrical portion 13 that supports the pusher 15.
In some cases, communication holes 8 are provided in the circumferential walls of the fitted portions 16 of the inner cylinder 5 and the inner cylinder 5 so that they communicate only when they are brought into alignment by the rotation of the inner cylinder 5. In this case, since the communication hole 8 opens above the culture medium chamber 2, it becomes possible to use a powdered culture medium. In addition, when the communication hole 8 is made openable and closable, even if the internal volume of the suspension preparation chamber 1 is compressed due to an erroneous operation and the suspension 7 is to be dispensed into the culture medium chamber 2, the inner cylinder 5 may be closed. The suspension 7 will not flow into the culture medium chamber 2 unless the communication holes 8 are aligned by rotation.

Although an embodiment in which the instrument main body 3 is constituted by a double cylinder has been described with reference to the drawings, a piston-type instrument may be cited as an example not shown. The partition wall 14 that partitions the suspension preparation chamber 1 between the claw 9 and the outer cylinder 6 is made to be able to slide while maintaining liquid tightness with respect to the outer cylinder, while the cylindrical part 13 corresponding to the piston rod is A culture medium chamber 2 is formed between the cylindrical portion 13 and the outer cylinder 6, and a pusher 15 with a collection needle 4 attached thereto is accommodated within the cylindrical portion 13.

The sampling needle 4 is built into the main body with a structure such that it can be optionally exposed outside the main body and housed within the suspension preparation chamber 1. For example, as shown in FIGS. 1 to 7, a pusher 15 is provided that passes through the main body, and the collection needle 4 is attached to the tip of the pusher 15. In this case, in order to achieve a liquid-tight seal between the pusher 15 and the cylindrical portion 13 of the inner cylinder 5 that forms part of the mouth portion 9 and the communication hole 8, an O-ring or the like is fitted onto the pusher 15. A flange 17 that slides on the inner surface of the cylindrical portion 13 and the inner surface of the mouth portion 9 is integrally molded on the pusher 15.

When the pick and collection needle 4 are not drawn into the suspension preparation chamber 1, the mouth portion 9 and the cylindrical portion 13 are closed by the flange 17 of the pusher 15. Therefore, the liquid 7 contained in the suspension preparation chamber 1 will not leak. By this method of supporting the collection needle 4, the collection needle 4 is stored in the suspension preparation chamber 1 by grasping and pulling it up. Incidentally, a stirring blade 18 is formed near the collection needle 4 of the pusher 15 to create a suspension preparation chamber! The distilled water inside the container may be stirred.

In addition, the pusher 15 with the sampling needle 4 attached to the tip is inserted into the instrument body 3.
It is also possible to provide a spring support within the suspension preparation chamber 1 so that the suspension preparation chamber 1 is pulled into the suspension preparation chamber 1 by the force of the spring 19 after collection. For example, communication holes 8 are bored in the inner circumferential wall of the inner cylinder 5 forming the plurality of culture medium chambers 2 and in the circumferential wall of the cylindrical part 13 integrally formed with the partition wall 14, and each communication hole 8 is formed by the rotation of the inner cylinder 5.
FIG. 8 shows an embodiment in which a spring-supported sampling needle is incorporated into a device main body 3 of the type in which the communicating hole 8 is opened and closed by aligning or shifting the needles. In this embodiment, the pusher 15 is slidably housed in the cylindrical portion 13 that forms part of the communication hole 8, and the coil spring 1 attached to the rubber cap 20
It is supported by 9. The rubber cap 20 is attached to the inner cylinder 5
If pressed, it will collapse, but if the force is removed, it will recover and bulge upward, that is, in the direction of pulling up the pusher 15.

Here, the coil spring 19 functions to support the pusher 15 in a floating state and to transmit the movement of the rubber cap 20.

In the case of this embodiment, even if the stirring blade 18f is formed on the pusher 15 itself, it cannot be rotated, so a stirring ball 21, such as a glass ball, is housed in the suspension preparation chamber 1. is possible. In this case, the content liquid is mixed by simply shaking the test instrument main body due to the stirring action caused by the movement of the ball 21. For stirring? - The rotor 2 is not limited to the embodiment shown in FIG. 8, and may be implemented in combination with the stirring blade 19 or alone.

The main body of the test instrument for identifying microorganisms of the present invention described above may be formed of any material, but it is preferable that at least the 2 portions of the culture medium chamber be formed of a transparent material. In this way, the culture state of microorganisms and the biochemical properties shown in the culture medium etc. can be observed just by looking at the test instrument for identifying microorganisms of the present invention.

(2) Functions and Effects of the Invention Since the microorganism identification test instrument of the present invention is constructed as described above, the operations of collecting, suspending and diluting microorganisms, dispensing, and culturing microorganisms are performed as follows.

First, a test instrument for identifying microorganisms of the present invention, which is pre-filled with distilled water and various culture media, is prepared. Then, the rubber cap of this microorganism identification testing device is removed, the collection needle is exposed outside the main body, and a colony of pure cultured microorganisms is collected. Next, a rubber cap is fitted to the opening to seal the suspension preparation chamber, and then the collection needle is pulled into the suspension preparation chamber and stored. Distilled water is stored in the suspension preparation chamber in advance, so the collected microorganisms are suspended in the distilled water by shaking the device, and then the collection needle is pulled up to transfer the suspension into the culture medium chamber. to pump. The internal volume of the suspension preparation chamber is reduced by pushing down the inner cylinder that forms the culture medium chamber, or by compressing the bellows-shaped suspension preparation chamber. The suspension extruded from the suspension preparation chamber is dispensed into the plurality of culture medium chambers through the communication holes.

The microorganisms contained in the suspension dispensed into each culture medium chamber are
It is cultured under the medium stored in this medium chamber in advance, and reacts with the substrate etc. contained in the medium.
Its biochemical properties are shown.

As described above, the microorganism identification testing device of the present invention integrates a culture medium chamber that partitions a plurality of culture media and a suspension preparation chamber that stores distilled water and dilutes collected microorganisms to a certain concentration into one device. The suspension preparation chamber is configured to have a structure in which the suspension liquid is formed in the suspension preparation chamber and to communicate with each other, and the internal volume of the suspension preparation chamber is made variable so that the suspension liquid in the suspension preparation chamber flows into the culture medium chamber. By simply compressing the internal volume of the suspension, a diluted suspension with a constant concentration can be dispensed into a plurality of media at once in this suspension preparation chamber. Therefore, conventional dispensing operations using instruments such as pipettes require opening, sterilizing, and capping operations for each test tube containing a different culture medium, which is complicated. The dispensing operation time can be dramatically shortened because the process can be completed all at once by simply compressing the suspension preparation chamber without requiring any operations. In addition, the dispensing operation is simple, requiring only the compression of the suspension preparation chamber, so no skill is required and even beginners can handle it without failure. Furthermore, since the suspension is dispensed by moving the liquid within the same container, there is no risk of contamination with bacteria.

In addition, since the collection needle is exposed outside the test instrument body and can be retracted and stored in the suspension preparation chamber, each operation of microorganism collection, suspension dilution, dispensing, and culturing can be performed continuously in one step. It is efficient because it can be carried out in a separate container and the trouble of preparing and transferring various instruments is saved.

In particular, the identification testing device of the present invention can store medium and distilled water distributed in appropriate amounts in advance in a sterile state, reducing the labor required for actually collecting and culturing microorganisms.・Time can be reduced.

In addition, at least the culture chambers are made of transparent material and are adjacent to each other, so it is possible to compare and observe the culture state of microorganisms or changes in the properties and color tone of the culture medium due to microorganisms without having to take out each culture chamber. can.

Furthermore, if the suspension preparation chamber is equipped with a stirring goal, the suspension can be mixed by simply shaking the test instrument main body due to the stirring action caused by the movement of the stirring ball.

In addition, if stirring blades are formed on the pusher that supports the sampling needle, the suspension can be removed by pressing a rotating body against the head of the pusher and rotating the stirring blades without shaking the test instrument itself. can be mixed.

In addition, if the collection needle is supported by a spring so that it can be pulled up into the suspension preparation chamber by the force of the spring, it is convenient to simply push out the collection needle when collecting.

[Brief explanation of drawings]

FIG. 1 is a central vertical cross-sectional view showing one embodiment of the microorganism identification testing device of the present invention, in which (a) shows the collection state, (b) shows the state with the collection needle pulled up, and (C) shows the suspended state. Each shows the dispensing status of the liquid. FIG. 2 is a cross-sectional view showing a circular culture medium chamber, and FIGS. 3 and 4 are a cross-sectional view and a vertical cross-sectional view showing an embodiment of a square culture medium chamber. FIG. 5 is a central longitudinal cross-sectional view showing another embodiment of the present invention, in which (a) shows the collection state, (b) shows the state with the collection needle pulled up, and (c) shows the suspension dispensing state. FIG. 6 is a drawing showing another embodiment of the present invention, in which (a
) shows the state of collection, (b) shows the state with the collection needle pulled up,
(c) is a central vertical cross-sectional view showing the state in which the communication hole is opened, (d) is a central vertical cross-sectional view showing the dispensing state of the suspension, (e) is a cross-sectional view along XX, and (f) is a cross-sectional view along Y-Y. It is a diagram. Figure 7 shows the structure of the present invention.
In the drawings showing other embodiments, (a) shows the collection state, (b)
(C) shows the state in which the collection needle is pulled up, (C) shows the state in which the communication hole is opened and the suspension is dispensed, and (d) shows the state in which the communication hole is closed to prevent backflow of the culture solution. FIG. 5 is a central vertical cross-sectional view showing each state, (e) is a zz cross-sectional view, and (f) is a w-w cross-sectional view. FIG. 8 is a central longitudinal cross-sectional view showing another embodiment of the present invention, (a) shows the collection state, (b) shows the state with the collection needle pulled up, and (C) shows the suspension dispensing state. are shown respectively. In the drawings, 1... Suspension preparation chamber, 2... Culture medium chamber, 2a
... circular culture medium chamber, 2b ... square culture medium chamber, 3 ... main body, 4 ... collection needle, 5 ... inner cylinder, 6 ... outer cylinder, 7 ... suspension, 8...Communication hole, 9...
Mouth part, 10... Cage, 11... Rubber membrane, 12.
... Culture medium, 13... Cylindrical part, 14... Partition wall, 15.
... Pusher, 16... Fitted part, 17... Franz, 1
8... Stirring blade, 19... Spring, 20... Rubber cap, zo. Patent applicant Terumo Corporation Figure 1 (a (b)
(C) Figure 6 Figure 6 Figure 7

Claims (9)

[Claims] (1) It has a suspension preparation chamber whose internal volume can be changed, a plurality of culture medium chambers storing mutually different culture media, and a communication hole forming a liquid flow path from the suspension preparation chamber to the culture medium chamber. A test instrument for identifying microorganisms, comprising a main body and a sampling needle that allows the main body to be exposed to the outside and stored in the suspension preparation chamber. (2) The partition wall that partitions the suspension preparation chamber and the culture medium chamber of the main body is configured to be movable in a liquid-tight manner, so that the internal volume of the suspension preparation chamber can be changed. The test instrument for identifying microorganisms according to item 1. (3) A patent in which at least a part of the main body constituting the outer wall of the suspension preparation chamber is made of a deformable material, and the internal volume of the suspension preparation chamber can be changed by pressing. A test instrument for identifying microorganisms according to claim 1. (4) A suspension preparation chamber is formed at one end side of the main body, and a plurality of mutually partitioned culture medium chambers are formed on the partition wall or at the other end side of the partition wall that partitions the suspension preparation chamber. A test instrument for identifying microorganisms according to any one of claims 1 to 3, which is provided in contact with a partition wall. (5) The test instrument for identifying microorganisms according to any one of claims 1 to 4, wherein the communicating hole is openable and closable. (6) The test instrument for identifying microorganisms according to any one of claims 1 to 5, wherein a plurality of communication holes are provided to communicate each medium chamber and the suspension preparation chamber. (7) A pusher is movably provided in the main body,
The test instrument for identifying microorganisms according to any one of claims 1 to 6, wherein the tip of the pusher is a collection needle. (8) The main body includes a cylindrical body having a closed end face at one end and an opening that can be opened and closed on the end face, and a partition wall in which a cylindrical part with both ends open is connected to the inside of the cylindrical body. is arranged so that it can slide liquid-tightly on the inner wall of the cylindrical body, and between the partition wall and the inner surface of the cylindrical body, a suspension whose internal volume can be changed is placed on the mouth side of the cylindrical body. A culture medium chamber is provided on the end surface of the partition wall not facing the opening, and a pusher whose tip is a collection needle is placed inside the cylindrical portion so as to form a preparation chamber. Claims 2, 4, 5, or 7, wherein the inner wall of the sampling needle is arranged so as to be slidable in a fluid-tight manner, and the sampling needle is exposed from the mouth. Test instrument for identifying microorganisms as described. (9) The main body includes a cylindrical body having a closed end face at one end, an opening that can be opened and closed on the end face, and a side surface near the end face made of a deformable material; In the cylindrical body, a partition wall connecting a cylindrical part with both ends open from the side surface near the end face to the open end side is provided, and the partition wall and the inner surface of the cylindrical body are used to prevent content from entering the mouth side of the cylindrical body. A suspension preparation chamber whose volume can be changed is formed, a culture medium chamber is provided on the side of the partition wall not facing the mouth, and a pusher whose tip is a collection needle is inserted into the tube. The microorganism according to any one of claims 3 to 7, wherein the inner wall of the shaped part is arranged so as to be slidable in a liquid-tight manner, and the collection needle is exposed from the mouth part. Testing equipment for identification. 00 A communication hole communicating with the culture medium chamber is provided in the cylindrical part,
The test instrument for identifying microorganisms according to claim 8 or 9, wherein the communication hole is opened and closed by vertical movement of the pusher. 01) A fitted part into which the cylindrical part is fitted is provided, communication holes are provided in the fitted part and the cylindrical part, and the communication holes are opened and closed by rotating the fitted part or the cylindrical part. A test instrument for identifying microorganisms according to claim 8 or 9. αa A rotating part having a culture medium rotatable in the circumferential direction is provided in a cylindrical part on the partition wall, and communication holes are provided in each of the rotating part and the partition wall, so that the rotating part can be rotated. Claim 8 or 9 in which communication holes can communicate with each other
Test equipment for identifying microorganisms as described in section. (li) The test instrument for microorganism identification according to any one of claims 8 to 12, which is configured to slide by pushing down or pushing up the pusher. A test instrument for microorganism identification according to any one of claims 8 to 12, which is fixed and configured to push down the pusher by pressing the spring. The test instrument for microorganism identification according to any one of claims 8 to 14, which is provided with a stopper and opens and closes the top part by attaching and detaching the lid or the stopper.0Q The mouth part is covered with an elastic membrane. The testing device for identifying microorganisms according to any one of claims 8 to 15, wherein the elastic membrane is penetrated from the inside with a sampling needle at the tip of a pusher to open the neck part. 17. The test device for identifying microorganisms according to any one of claims 1 to 16, wherein at least a portion of the main body is substantially transparent, and the inside of the culture medium chamber can be observed from the outside. A testing device for identifying microorganisms according to any one of claims 1 to 17, which is a culture medium. The test instrument for identifying microorganisms according to any one of Item 18.Claim 8. A stirring plate is provided on the pusher.
The test instrument for identifying microorganisms according to any one of Items 1 to 19.