JPS581795Y2 - Biogas metabolism measuring device - Google Patents

Description

[Detailed explanation of the invention] This invention aims to make it easy to carry out rigorous measurements of gas metabolism associated with respiration and photosynthesis in living organisms, and to achieve the educational purpose of deepening understanding of respiration and photosynthesis, which are important functions of living organisms. It is something to do.

The present invention will be described in detail with reference to the embodiment shown in the drawings. After a groove is dug in a part of the upper surface of a horizontal member 1 such as a synthetic resin square timber, a capillary space 3 is formed by closely adhering a plate 2 made of synthetic resin or the like.

Capillary holes 4, 5 are branched from one end and the center of this capillary space 3, openings 6, 7 are provided on the lower surface, and short capillaries 8, 9 are adhered to each opening 6.1, respectively.

The other end of the capillary space 3 is opened at the upper surface to form a small hole 10.

A small hole 12 is formed on the upper surface by penetrating the small hole 11 from the upper surface to the lower surface immediately adjacent to the small hole 10, and a capillary tube 14 is attached to the opening 13 on the lower surface.

The capillary 5 leading from the center of the capillary space 3 to the capillary 9 on the lower surface and the pore 11 leading from the small pore 12 on the upper surface to the capillary 14 on the lower surface are each provided with branched pores 15 and 16 in the center and on the side. Openings 17.18 are provided and a capillary tube 19, 20 is glued into each of these openings 17.18, respectively.

Both ends of the U-shaped tube 210 are connected to the capillary tube 9 and the capillary tube 14, respectively, and colored liquid is injected through the small hole 12 with a syringe or the like to form droplets 22 in the U-shaped tube 21.

Two fixed lines 23 and 23' are cut into the U-shaped tube 21.

A graduated capillary tube 24 shaped like a measuring pipette is connected to the capillary tube 4 to form a volume meter 25.

Furthermore, a liquid reservoir 26 made of a pressure-resistant rubber tube or the like is connected to the lower end of the liquid reservoir 26, and a colored liquid 27 is sealed inside the liquid reservoir 26.
By pressurizing the liquid reservoir 26 at step 8, an arbitrary amount of colored liquid 21 is added.
is introduced into the tube of the volumetric meter 25 to form a liquid column 29.

The capillary tube 19 and the capillary tube 20 have capillary rubber tubes 30°31
are connected to each other, and capillary tubes 34 and 35 that terminate at the fitting portions 32 and 33 are connected to the other ends of these capillary rubber tubes.
Connect each.

A reaction container 36 and a control container 37 are connected to the capillary tube 34 and the capillary tube 35, respectively.

The present invention is realized as described above, and to use this, taking the case of using aquatic plants as an example, the volume is 20 to 30.
Pond or lake water or a dilute carbonate buffer solution is poured into the reaction container 36 of -, and approximately 5 - of the leaves of aquatic plants are poured into the reaction container 36, and about the same amount of the solution is poured into the control container 37.

The reaction container 36 and the control container 37 are respectively connected to the joint portion 32 of the capillary tube 34 and the joint portion 33 of the capillary tube 35 using grease, and both containers are immersed in water in a constant temperature water bath (not shown) and shaken.

After about 10 minutes, tilt the device to align the left end of the droplet 22 in the U-shaped tube 21 with the fixed line 23, then close the small hole 10° 12 at the same time with a piece of vinyl tape, return the device to the vertical position, Turn the adjustment screw 28 to accurately align it with the fixed line 23 at the left end of the droplet 22, and read the position of the tip of the liquid column 29 on the scale.

This time is set as 0 o'clock, and thereafter the operation of turning the adjustment screw 28 at regular intervals (for example, 5 minutes) to align the left end of the droplet 22 with the fixed line 23 and reading the position of the tip of the liquid column 29 is repeated.

If the intensity of the light irradiating the reaction container 36 is above the compensation point of the aquatic plants, the leaf pieces of the aquatic plants will absorb carbon dioxide dissolved in the solution in the container and generate oxygen, so the droplet 22 will move to the right. However, by loosening the adjustment screw 28 and appropriately lowering the position of the tip of the liquid column 29 in the volume meter 25, the left end of liquid #J22 can be brought back to its original position, that is, to the fixed line 23. It can be returned.

By such an operation, if the position of the tip of the liquid column 29 is lowered by a certain section in the tube of the volume meter 25 after a certain time from 0 o'clock, the oxygen corresponding to the volume inside the tube in that section will be lowered from 0 o'clock to that time. It can be assumed that the water was generated from the aquatic plants in the reaction vessel 36.

The amount can be read from the scale of the volume meter 25, and if it is vut and the temperature inside the tube of the volume meter 25 (equal to room temperature) is t°C, then the standard state of the amount of oxygen generated is 0°C / 1 atm. ) is obtained as v×−”no knee.

273 + As mentioned above, the main features of this proposal are as follows.

In the conventional device based on the principle on which this project is based, the main part, which was made of glass capillary tubes, was changed to a structure that can be made of synthetic resin, and the special stopcock used in the past was also abolished, making it quite complicated. Since there is no need to operate a stopcock, the device of the present invention is less likely to be damaged, has a low floor, and can be provided with an extremely easy-to-operate device.

Moreover, the measurement accuracy is the same as that of the conventional type, and it is possible to realize simple and highly accurate measurements of photosynthesis and respiration in the field of biology education at the high school level.

[Brief explanation of drawings]

The figure is a perspective view of the proposed metabolism measuring device. 1 is a horizontal member, 2 is a plate, 3 is a capillary space, 4 is a capillary hole, 5
is a capillary, 10 is a small pore, 11 is a pore, 12 is a small pore, 15
, 16 is a branched pore, 21 is a U-shaped tube, 22 is a droplet, 25 is a volume meter, 36 is a reaction vessel, and 37 is a control vessel.

Claims (1)

[Scope of utility model registration request] One end of a horizontal capillary space sealed inside a horizontal member such as a synthetic resin square timber is opened on the upper surface to form a small hole,
One opening on the side surface and two openings on the bottom surface are used as openings, and one opening is made on the side surface of the pore that penetrates vertically near the small hole on the top surface to form an opening. A volume meter is connected to the lower opening at the end, each end of a U-shaped tube with a scale is connected to the lower opening in the middle of the capillary space and the lower opening of the pore, and the side opening of the capillary space is connected to the volume meter. A biogas metabolism measuring device in which each of the side openings of the pores is communicated with a reaction vessel or a target vessel via a capillary rubber tube, so that the pores in the capillary space and the pores in the pores can be freely closed.