JPS5817726B2 - Composition for controlling tree blight and rot - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a composition for controlling tree blight and rot;
For details: 3,5-dimethyltetrahydro-2H-1,3
- It relates to a bactericidal composition characterized by containing 5-thiadiazine-2-thione as an active ingredient.

Tree blight diseases include Valsa ceratosperma and Phomopsis truncicola of fruit trees, especially apples.
Miura), pear blight (Phomopsis f.
ukusnii Tanaka et Endo), mulberry blight (Diaporthe nomurai)
Hara), chestnut blight (Endothia Pa
rasitica P, J, et H, W, Ande
rson) and other trees, paulownia rot ('Va
lsapaulowniae Miyabe etH
emmi), but these blighting diseases are caused by insufficient cultivation management due to recent lack of labor, lack of appropriate and effective control chemicals, and due to the characteristics of the disease, bacterial Because the parasitic and diseased parts of the eyes may extend deep into the bark layer, it is difficult to control the insects because it is difficult for general insecticides to be effective, or high-performance insecticides such as speed sprayers are required. It is a known fact that the areas affected by these diseases are widespread and the importance of control is increasing year by year, mainly due to difficulties in control such as lack of careful spraying due to dependence.

In particular, for perennial crops such as fruit trees, although the amount of effort and expense involved in cultivation management up to the point of conflict is considerable, the disease caused by these blighting diseases can significantly reduce production volume and tree vigor. (In cases where the number of occurrences is extremely high, it may even lead to the closure of the park.)

In particular, the damage caused by leaf blight on apples is enormous, and there is a strong need for the development of appropriate control techniques and control agents for these blight-related diseases.

From this perspective, the present inventors conducted extensive research on searching for effective drugs against various blight diseases, and found that 3,5-dimethyltetrahydro-2H-1,3,5-thiadiazine-2-thione We have discovered the fact that a preparation containing TMTT (hereinafter referred to as TMTT) as an active ingredient exhibits a surprisingly significant pesticidal effect without causing any practical harm to trees.

The present inventors have further discovered that a composition in which a fixation adjuvant is added to the effective composition TMTT of the present invention exhibits a more stable effect against body blight and rot, and exhibits a remarkable effect.

TMTT, which is the active ingredient of the composition for controlling tree blight of the present invention, is known as a soil disinfectant, and when this compound is applied to soil, it hydrolyzes and generates a bactericidal and insecticidal effective gas. Although it shows a controlling effect on soil diseases and nematode weeds, no attempt has yet been made to eliminate these diseases by directly applying it to the affected areas of trees, such as body blight and rot. There isn't.

Generally, when applying chemicals to treat apple rot, it is customary to cut the diseased area and apply the chemicals to the cut area and its surroundings, but this cutting process requires a great deal of labor and is difficult to prevent serious diseases. This is one of the factors that makes it difficult.

The present composition for controlling blight disease is characterized in that the above-mentioned cutting operation is not performed, and a chemical solution of the present composition at a predetermined concentration can be applied or sprayed onto the diseased area.

In this case, the period of use is limited to before the tree germinates or after harvest in the case of fruit trees, but by applying or spraying this composition, it will not have a practical adverse effect on other healthy tissue parts ( In order to kill and sterilize only unnecessary diseased tissues and to enable efficient control, control using such control compositions has a large effect on simple and efficient control compared to conventional control methods. There are advantages.

Originally, this effective compound was applied only to the soil as mentioned above, and the hydrolyzed gas was said to be effective.

In particular, the half-life of this active ingredient in soil is extremely short, ranging from half a day to one day.

Therefore, when the present inventor completed the present invention, the most important point of research was how to suppress the volatility and provide a sustained bactericidal effect when sprayed on trees.

In other words, in view of the properties of TMTT, the present inventors have conducted various tests and studies, and have found that by adding auxiliary ingredients as described below to the TMTT main ingredient at the time of use, or by adding auxiliary ingredients to the TMTT main ingredient in advance. It was discovered that the effect was further increased by keeping it in place, and the effect was further stabilized, exhibiting a remarkable reinforcing effect.

The auxiliary agent that enhances the effect mentioned above is one that is added at the time of use or mixed with TMTT in advance as described in the examples, and fixes or covers the TMTT to further enhance the effect. Various types contributed to enhancing the effect, depending on the type.

That is, a polymer emulsion such as paraffin, vinyl acetate, polyoxyethylene resin acid, polyoxyethylene alkyl ether, or synthetic resin, or one type of vegetable oil, polyvinyl alcohol, alginic acid, gelatin, sodium carboxymethyl cellulose (CMC), etc. The efficacy could be enhanced by adding two or more types.

That is, when actually producing and using the present invention, it is possible to formulate and use the usual dosage forms commonly used as agricultural chemicals at present, as described in the Examples.

In addition, when an adjuvant is added to the above-mentioned ordinary preparation, it can be easily prepared by adding it at the time of use.
It is also possible to prepare and use a mixed agent with MTT.

It was completely unexpected and completely unknown that such a preparation and method of use would exhibit excellent effects against rot and blight, and would also exhibit excellent practicality.

This pest control agent and its method of use not only significantly simplify and save labor for pest control work, but also make it possible to alternately use compounds with completely different effects from conventional pest control agents, along with its excellent pest control effect. This makes it extremely useful in taking measures against resistant bacteria.

Next, Examples will be described in order to explain the present invention in detail, but it goes without saying that the proportions of each compound can be varied over a wide range other than those shown below.

Example 1 20 parts by weight of aqueous solution TMTT (98%), caustic soda (95%)
) 11 parts by weight and 69 parts by weight of water were mixed and dissolved to obtain an aqueous solution.

When using, dilute with water and apply with a brush.

Example 2 Parts by weight of emulsion TMTTIO were added to N-N-ditylformamide (
The mixture was dissolved in 44 parts by weight of DMF (hereinafter referred to as DMF) and 40 parts by weight of xylene, and 6 parts by weight of an emulsifier was added thereto and mixed and stirred to obtain an emulsion.

Diluting this with water produces a good emulsion.

Apply this dilution to the affected area by spraying or brushing.

Example 3 5 parts by weight of oil TMTT, 20 parts by weight of DMF and 55 parts by weight of xylene
Parts by weight were dissolved in 20 parts by weight of linseed oil to obtain an oil solution.

This may be directly sprayed using a sprayer or applied with a brush.

Example 4 An irrigation agent was obtained by mixing and pulverizing 40 parts by weight of TMTT, 40 parts by weight of clay, 10 parts by weight of bentonite, and 10 parts by weight of a dispersant.

This is diluted and suspended in water and sprayed or applied.

Example 5 Powder TMTT 4 parts by weight, talc 89 parts by weight, gelatin powder 4
1 part by weight of a dispersant and 2 parts by weight of sodium carboxymethyl cellulose (CMC) were added to the weight part and mixed and pulverized to obtain a powder.

Add water to this and mix well to form a paste and apply it to the affected area.

Example 6 3 parts by weight of aerosol agent TMTT, 34 parts by weight of DMF, 33 parts by weight of Kijirole
10 parts by weight of pigment and 20 parts by weight of propellant were added and filled to obtain an aerosol agent.

Spray this aerosol onto the affected area.

Example 7 Paste agent 50 parts by weight of boiling oil, 40 parts by weight of pigment, and 5 parts by weight of dispersant were mixed with 5 parts by weight of TMTT, and a sufficiently uniform paste agent was obtained using a homogenizer.

Apply this paste directly to the affected area.

Next, the characteristics and control effects of the composition for controlling tree blight diseases of the present invention and the control method using the same will be explained in more detail by giving test examples.

In the following test examples, except for the application method in Test Example 4, the others were conducted by dipping, but all of the various test methods using this dipping method are effective against apple rot, pear blight, and mulberry blight. This is a well-known basic test method necessary for determining the practical effectiveness of coating agents and spraying agents, and has been established as a general test method for determining the practical effectiveness of coating agents and spraying agents against these diseases. This is the way to do it.

Test example 1 Mycelial invasion prevention effect test against apple rot fungi A wettable powder prepared according to Example 4 was tested.

12cr of apple new shoots (variety Dorin) with a thickness of about 1crrL
It was cut to rL length and used as a sample.

The test was conducted with one continuous system of four branches in one district.

After making a burn wound in the center of the test branch, it was immersed in a chemical solution of a predetermined concentration for 5 minutes, air-dried, and then left indoors.

As control chemicals, a lime sulfur mixture was used in the first test, and agricultural formalin and benomyl irrigation agent were used in the second test.

On the day of immersion in the chemical solution and 4 days later, the flora of flora which had been cultured in advance was punched out using a 5 m 7 ft cork pole, inoculated onto the burnt area, wrapped with cellophane tape to make it adhere, and then incubated under greenhouse conditions for 25 minutes. It was left in a constant temperature room.

The first test was conducted 11 days after treatment, and the second test was conducted after treatment 1.
Two days later, the length of each lesion was measured excluding the withered area.

1st exam Test example 2 Hyphal elongation inhibition effect test against apple rot fungi An aquarium prepared according to Example 4 was tested.

Apple new shoots (variety Dorin) with a thickness of about ICrfL are 12Cr
It was cut to rL length and used as a sample.

One district has four branches, one in a row.

A formalin solution benomyl water conservancy was used as a control drug.

A pre-cultured bacterial flora of rot rot was punched out using a 5 mm cork pole, inoculated onto the burned area, wrapped with cellophane tape to make it stick tightly, and then inoculated under greenhouse conditions for 2 days.
It was left in a constant temperature room at 5°C.

Three days after inoculation, the tips of the lesions that had spread were peeled to a width of 5 mm, immersed in a solution of the test drug at a predetermined concentration for 5 minutes, air-dried, and then returned to a wet room condition.

The investigation was conducted by measuring the length of the lesions extending from the peeled area 7 days after drug treatment.

Test example 3 Treatment effect test for apple rot disease An emulsion prepared according to Example 2 was tested.

12cr of apple new shoots (variety Dorin) with a thickness of about ICrrL
It was cut to rL length and used for testing.

1 ward, 4 branches, 1 consecutive system.

Formalin solution was used as a control drug.

A pre-cultured apple rot bacterium was punched out with a 5 mm cork polar and inoculated onto the burned area.
It was wrapped tightly with cellophane tape and left in a constant temperature room at 25° C. under greenhouse conditions.

Three days after inoculation, the inoculated test branches were immersed in each chemical solution at a predetermined concentration for 5 minutes, air-dried, and then returned to a wet room condition.

The investigation was conducted 7 days after the drug treatment, and the length of each lesion that had spread after the drug treatment was measured.

During the investigation, the tissue of the lesion was surface sterilized with 75% alcohol, cultured in PDA medium, and the growth of hyphae of S. rot fungi was investigated.
The presence or absence of bactericidal effect was investigated.

Test Example 4 Field effect test against apple rot An emulsion prepared according to Example 2 was tested.

We tested rot lesions that appeared on apples (variety Fuji) between 10 and 15 years old.

20 lesions in 1 area were tested.

In the spring (April), the rotten rot diseased area (body rot) is scraped off in a spindle shape, and 5 days later, the chemical is applied to the healthy areas as well.

In the fall of that year (mid-October), the healing status, degree of callus formation, and presence or absence of drug damage were investigated.

Callus formation rate Ratio of callus formation area when the circumference of the lesion from which two drugs were removed is taken as 100.

Formation degree of callus: surrounding swelling degree 0.12.3
The survey was divided into four stages, and the average value was calculated.

Test example 5 Effect test on pear blight An emulsion prepared according to Example 2 was tested.

20 crt of pear branches (variety Kosui) with a thickness of about 1 crrL
I cut it to length L and used it for testing.

The test was conducted with one continuous system of four branches in one ward.

Bacterial plaques previously cultured in PDA medium were inoculated into the burnt area and left in a constant temperature room at 25°C.

Three days after inoculation, the specimens were immersed in a solution of a given concentration of the test drug for 5 minutes.

The investigation was carried out 7 days after the drug treatment, and the length of the lesions that had grown after the drug treatment was measured.

Test example 6 Effect test on mulberry blight An emulsion prepared according to Example 2 was tested.

12c of Kuwakojo (variety: -nose) that has been kept cold for several months
Cut to length rrL and test.

The test branches were lightly sterilized with 75% alcohol, and the cut ends were sealed with paraffin.

Using an electric trowel, apply 17ILm x 1m1 to three locations on the test branch.
A burn wound was made at rt, and stalk blight fungal stalk spores (bacterial solution concentration 107/ml) were inoculated into this area.

Inoculation was carried out by applying a bacterial solution to the tips of 10 sewn pots and lightly pricking them.

Immediately after inoculation, the seeds were kept in a greenhouse at 23°C, and 3 days after inoculation, they were immersed in a chemical solution of a predetermined concentration for 10 minutes, air-dried, and then left in moist sawdust.

The investigation measured the length of lesions that developed 10 days after treatment.

Claims (1)

[Claims] 13,5-dimethyltetrahydro-2H-1,3,5-
A composition for controlling tree blight and rot, containing thiadiazine-2thione as an active ingredient. 23,5-dimethyltetrahydro-2H-1,3,5-
A composition for controlling tree blight and rot, which contains thiadiazine-2-thione as an active ingredient and also contains a fixing agent.