JPS58171401A - Treatment of cellulose-containing substance - Google Patents

Abstract

PURPOSE:A cellulose-containing substance is previously treated with polyethylene glycol, then saccharified with cellulose to effect markedly improved saccharification properties. CONSTITUTION:A cellulose-containing substance such as farm by-products is dipped in a polyethylene glycol or its derivative solution of usually 0.1-10%, preferably 0.1-1% concentration above room temperature, usually at 30-100 deg.C and 4-10pH. The mixture is filtered to recover the cellulose-containing substance and the substance is directly or, when necessary, after washed with water, dried to prepare the material to be saccharified. Then, a cellulose preparation such as from Trichoderma is made to act on the material for saccharification.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for pretreating a cellulase-containing material for saccharifying the cellulose-containing material into cellulase.

In order to produce alcohol as an alternative fuel and conserve food resources, research is being actively conducted to produce glucose by saccharifying cellulose. Cell field
In nature, it exists in a strong bond with lignin and heliase.

In Soyama, various methods have been devised to remove lignin and hemicase O, and to destroy the crystal structure of cellulase, in order to make it more susceptible to decomposition by cellulase. For example, there are methods of treating cellulose-containing materials with acids such as sulfuric acid or phosphoric acid or alkalis such as caustic soda, methods of treating them with R-rays, and methods of pulverizing them with a pole mill. Freezing and thawing methods are known.

The present inventors have conducted extensive studies on a method for treating cellulose-containing materials that promotes saccharification by cellulase, with the aim of producing glucose by saccharifying cellulose-containing materials with cellulose. It has been stated that when organic matter is treated with polyethylene dalicol or its derivatives, the ability to convert sugar into sugar by cellulase is significantly improved.The present invention was made based on the knowledge of ζO be.

That is, the present invention is directed to, seventhly, polysizing the cellulose-containing material when saccharifying the cellulose-containing material with cellulase.

The present invention will be explained in more detail below.

The cellulose-containing substances to which the present invention is applied are woody or non-woody (vegetable) cypress objects or derivatives thereof, such as agricultural by-products such as rice straw, Japanese mullet, and bagasse; Examples include weeds such as navy and cogon, wood, pulp, waste paper, and senile leafy materials, and are particularly effective against plant materials such as milk bagasse. These cellulose-containing substances do not need to be removed completely, and may be removed by mild chemical treatment, grinding, or
It is effective when used in combination with materials that have been subjected to physical treatments such as radiation treatment, high-temperature heat treatment, and freeze-thaw treatment. Polyethylene glycol 1ooo, preethylene glycol, 2
000. Polyethylene Dalycol aooo, Polyethylene Dalyseal 20000 (both sold by Wako Pure Chemical Industries, Ltd.), etc. (-0CHxCH*) are species AltJ heavy products having the structure as a unit.

As a derivative of ethylene glycol, Tween J
7. Tween O, Tween dO1 Queen lO, Tween tS (all manufactured by Atlas Powder Company of America), or Triton Z-too or Triton X-
These are derivatives having a skeleton of (0CHsCH-), such as Tei 01 (all of the above structures are manufactured by Rohm and Haas Company of America) and Bridge Jj (manufactured by Kao Atlas Co., Ltd., Kinsha).

These materials are usually used in a solution of about a/~104, usually Q/~l, and the cellulose-containing material is immersed therein. The dipping can be carried out at a temperature above room temperature, usually JO-100°C, but the dipping time can be shortened if the dipping temperature is high. OpH during immersion is effective at high VH, but normal pH
It is carried out in about 10 to 10 days.

After soaking, the cellulose-containing substances are collected by filtration, washed with water if necessary, and used as raw material for saccharification either as is or dried. Examples of cellulase enzyme preparations include Atricobium spp.
Cellular paddles of microbial origin can be used, such as those produced by the genus Aspergillus, Suborotrichum, and Irpex, and if necessary, these can be used in combination.

Next, the present invention will be specifically explained with reference to Examples.

Example 1 Bagasse (pulverized to about 12 &n') g) 100w1
9 was immersed in the 1% polyethylene glycol or surfactant solution listed in the table, incubated overnight at 30°C, filtered through a glass filter, thoroughly washed with water, and then dried. .

Add each SO■ of this to QJM vinegar laxative solution (pH yo > as
gLt, and add to this seven rulers ((Trichoderma viride seven rulerase enzyme, agent (Yakuru) manufactured and sold by Seikagaku Co., Ltd., Cellulase Onosuka R-to' ) Add 1 unit of each of the 11 mixed enzyme solutions and mix with water.

The reaction was allowed to take place at ℃ for 1 day. After the reaction, the filtered supernatant was extracted with triton x by the Somogyi-Nerlan method, soluble sugar by the phenol-sulfuric acid method, and glucose by the dulose oxidase method. -t o 41 Yotsu Toraton X-sanor, Bridge JU, Kizunayuki E+JA
When polyoxyethylene-based non-surfactants such as No. 1 were used, the conversion rate was significantly improved.・Effects were also observed with Matabori Ethylene Glyco-5tJoooo.

Note that cellulase activity was measured as follows.

QOjMfhWI lag solution (pHjO) was made to dissolve f#a
jlcMc (carboxymethylcellulose) solution aj@
Celltese enzyme solution was added to the solution, the total amount was made up to 1 gIt with distilled water, and the reaction was carried out at 50°C. The amount of enzyme that generates a reducing power equivalent to 1 micromole of glucose per minute under these conditions was defined as the unit of 1.

Example Kobagasu (pulverized to about Qj%) 100ml I9 soaked in water, 1%, 5%, 10GO)
Lydon
After being left overnight in Jj''C), it was filtered with a reduced EE filter using a glass filter, and then thoroughly washed with water and dried.

Each sample was saccharified at ℃ for one day by the method described in Example 1. After the reaction, the filtered supernatant was treated with reducing sugars, soluble sugars, and glucose. was quantified. The results obtained are shown in Table 2.

As is clear from Table #1, bagasse is prepared in advance)
The yield of be sugar was increased by about 20 to 30% when compared with the product treated with kx-san〇j and the product treated with this process. And the concentration of Triton X-san Oj is better than / attack! ≦〜l
O attack was more effective.Example J In Example 2, #IJ instead of Triton x-#os is shown in the table.

It is clear from Table J that the ring and bagasse are made of polyethylene = -
The amount of reducing sugars produced increased by about 1.3 times, and the amount of soluble sugars produced increased by about 7 times.

Example: 100 pieces of dabbagas (pulverized to about 0.000 kg) as a precautionary measure.9
) N
, 30°C and 70°C for 7 days each, and 100°C and 12°C.
Those at 0°C were heat-treated for each IO minute. After treatment, it was filtered, washed with water, and dried. In addition, bagasse tow 9 was immersed in water at 30° C. for 1 day, filtered, washed with water, and dried, which was used as a control.

This 0% 0 each jtOI + 9'kQ2M acetic acid mild polar solution (pHfO) was added to the Example! The same base material as used in 1 was added, the whole amount was diluted with water, and the mixture was reacted at 5°C for 2 days. After the reaction, reducing sugars, soluble sugars, and glucose were determined in the filtered supernatant. The obtained Ial
A is shown in the table below.

Claims (1)

[Claims] A method for treating a cellulose-containing material, in which the cellulose-containing material is preliminarily characterized by polyethylene and V when the cellulose-containing material is saccharified with cellulase.