JPS58162862A - Sample development method - Google Patents

Abstract

PURPOSE:To obtain a large amt. of fractioned sample component, by attaching a sample to the underside of the bottom of a chromatographic stick obtd. by forming silica gel with a binder, such as gypsum, and placing the stick on a stand in a solvent vessel. CONSTITUTION:An adsorbent, such as silica or alumina, is mixed with a necessary binder, such as gypsum, formed into a column or the like, and dried at about 100 deg.C. The obtd. stick 1 is placed on a stand 4 in a vessel 2 contg. a solvent, such as petroleum or benzene. A case 5 is used for the stand 4, and a proper number of through-holes 6 are formed through the circumference of the case 5 on the same level. A material 7 for lifting the solvent 3 is made of sponge or gauze, and its upper face is located above the holes 6. A sample 8 is attached to the underside of the bottom of the stick 1. The solvent 3 invades through the bottom into the stick 1 and develops the sample 8. Since the stick 1 can be enlarged in section, a high rate of fractioned sample component can be obtained without trouble in the development.

Description

[Detailed description of the invention] The present invention relates to a sample development method.

Recently, a glass plate is coated with a thin layer of silica gel, dried to form a thin layer of silica gel, one drop or two of the sample is applied to one end of the plate, and the sample is developed by immersing it in a solvent such as benzene or petroleum. A sample development method is used in which samples are separated using different rates of increase. However, in the case of the conventional thin gel layer formed on a glass plate (Bl), when the silica gel layer is thickened, there is a difference in the degree of adsorption between the part near the glass piece and the part far outside when the sample is developed. Because of this, there is a difference in the state of development against silica gel (
(Illustrated in Figure 1). For this reason, silica gel cannot be applied thickly, and the amount of samples collected is small. Furthermore, if silica gel was applied thickly, there was a risk of sample mixing during cutting for sample collection.

Therefore, in the present invention, the sample can be easily expanded and a large amount of sample can be collected. However, we are trying to propose an extremely practical method for developing the sample, which requires only one step to develop the sample by simply placing the stick on the stage. The method is characterized in that a sample is attached to the bottom of a chromatographic stick formed into a plate or column shape by mixing a binder such as gypsum, and the chromatographic stick is placed on a mounting table from which a solvent can be freely supplied.

The present invention will be described in detail with reference to examples shown in the figures below.
(1) A stick is used to mix an adsorbent such as silica gel, alumina, cellulose, or polyamide with a necessary binder such as gypsum and form it into a desired shape such as a columnar plate. The columnar shape, plate shape, etc. are referred to as a stick. The stick [1] is made by dissolving the material in water, forming it into a desired shape, and then drying it at about 110°C to strengthen its adsorption properties. (2) is a container that contains a solvent (31) such as petroleum or benzene, and a platform (4) is placed in the container.The platform (4)
is formed as a box (5), and around it, on the same plane or with an appropriate number of holes [61 (61...) are all drilled. (7) is a pumping object, made of sponge, gauze, asbestos, fl, formed of a material having capillary action, such as.

The top surface of the box (5) is placed above the hole (5+ (61)... (It enters through 61 (61...) and contains the solvent (3) throughout the pumping object (7). Next, we will explain how to use it. After that, place the entire stick (1) on the pumping object (7).If the stick (1) has a stable cross section such as a square or round shape.

Keep it upright, and if it is an unstable shape such as a plate, use a container (]
The side walls of 1 are raised and other supports are provided thereon to support the object, but to keep it as upright as possible. At this time, the solvent (3) leaches out from the bottom of the stick (1), and at the same time spreads the sample (8) attached to the bottom. If it is not located lower, only the solvent will rise. During this development, the sample (8) showed a difference in the degree of adsorption of each component to the stick (1), resulting in a separation layer r9) (91°).

is formed. After one sufficient separation is performed, each separation layer [
Cut the stick (1) every 91f91... to obtain a sample of each component. The obtained component sample is subjected to solvent extraction and other steps and used for qualitative or quantitative analysis as desired.

A pumping object (71) is placed on the Crane Buddha Q, and a pumping object ('11
It is also possible to use a structure in which the lower end or the descending part of the tube is immersed in the solvent (3) to infiltrate the pumping object tn@. Furthermore, the solvent (31) was placed on the platform (
According to the method of the present invention as described above, the stage is placed in a container containing S medium, and silica gel and, if necessary, plaster, etc. The chromatographic stick is mixed with a binder and formed into a plate-like or columnar shape so that the sample can adhere to the bottom surface and the solvent can be freely supplied.The chromatographic stick is placed on a single unit, which increases the cross-sectional area of the stick. It is possible to obtain a large amount of component samples due to its high sample adhesion resistance.However, it is possible to obtain a large amount of sample components due to the excellent separation performance. It has great practical effects,

[Brief explanation of drawings]

Figure 1 is a schematic illustration of the conventional method. Figure 2 is an axial view of the main parts of the same. Figure 3 is a vertical sectional view of one embodiment of the present invention. Figure 4 is a front view of another embodiment. Figure 6 is a front view of another embodiment. FIG. 2 is a perspective view of the stick in an expanded state. (1)... Stick (7)... Lifting object (2
)...Container (8)...Sample (3),...
Solvent (9)... Separation layer (4)... Mounting stage
QO)...Base (5)...Case
Ql) ... Leg (6) ... Perforation % Applicant: Gascro Industries Co., Ltd. 1 - Licensed patent attorney: Mitsuo Takahashi,
・Ah, 1 Fang 5 countries O 6 countries 363-

Claims (1)

[Scope of Claims] l) A mounting base is placed in a container containing a solvent, and silica gel and, if necessary, a binder such as gypsum is mixed in to make the sample stick to the bottom of a chromatographic stick formed into a plate or column shape. 2) A sample development method characterized by placing the above-mentioned chromatography stick on a platform which can freely supply chromatographic sticks. A method for developing a sample according to claim 1, characterized in that the sample is immersed in a solvent and its upper side is positioned above the solvent surface. 3) The solvent is supplied to the extent that it directly contacts the chromatographic stick on the stage. A sample method according to claim 1, characterized in that: