JPS58144746A - Separation of serum and blood clot - Google Patents

Abstract

PURPOSE:To improve the separation of serum and blood clot while the time required for the clotting of blood is reduced significantly by providing blood with an adsorbing inorganic substance subject to the absorption quantity of linsed oil, BET specific surface area and specific resistance within a specified range. CONSTITUTION:Blood is separated centrifugally into serum and blood clot. Prior to it, the blood is provided with an adsorbing inorganic substance subject to the absorption quantity of linsed oil of 20-40ml/100g, the BET specific surface area 5,000-30,000cm<2>/g and the specific resistance below 1X10<10>OMEGAcm at the surface temperature. The adsorbing inorganic substance herein used is, for example, fine silica powder.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for separating ms and blood clots, and more particularly to a method for separating serum and blood clots from a whole sample of a subject (by centrifugation).

In recent years, with the remarkable progress in testing technology, blood tests such as biochemical tests, immunological tests, and hematology tests have become possible.
It has become popular and has contributed greatly to the prevention and early diagnosis of diseases. Serum tests are the main component of -line tests, and the -KIL required for testing is usually the capacity for blood tests. After wave t-wao-ing, % dead weight varies depending on centrifugation.
Separated from rice cake (gel mass mixed with fibrin and spheres).

Then, the blood is centrifuged to separate serum and blood clots, 11 parts of the blood are sucked up with a pipette, and collected with a decantation bottle.-The groove part 0111& is performed after waiting for blood acid O coagulation.
The problem is that it takes a considerable amount of time to complete the test, and testing cannot be carried out quickly.

Quantity: 4 hours Self-sweeping S hard time sheet is said to be short. Even on the ISS, blood was injected and blood was injected into the air to cause coagulation.
It takes 0 to 60 minutes, and it takes 11 minutes for blood test screening.
KI Rite needs to be left alone at 4 o'clock in order to escape from the armpits.

Conventional serum and separation method on m furnace
1r'i, JlmL When the whole passageway is phase-separated into serum and blood clots of high specific gravity by means such as centrifugation, and a drop is collected in pure 1 for test Kl, the separability of the moment is generally poor. It is to be.

For this reason, you have to be careful not to let the red ball go up when you suck it up with Piit, and you have to be very careful not to let the red ball go in when you decant. However, in most cases red spheres were mixed in, which affected the critical test values), and there were other drawbacks such as the need for recentrifugation.

The drawbacks of the present invention are solved, and the I required for liquid coagulation is
? One of the hooks is to provide force swimming t#I that reduces mt large-KIli and satisfactorily separates ichisei kibu and gekimochi kibu.

The present invention 011Fi, - At the heart separation operation - How to separate serum and blood clot & 1 ICI? In the - line, the amount of linseed oil and sour oil is 0.46m/100f.

BET jt barley product is i... to 3...-/f.

characterized by the presence of an adsorbent inorganic substance whose specific resistance is usually less than I x 1010 Ω·1 at 11;
It consists in the method of separating serum and blood clots.

Next, the zero invention - method for separating serum and blood clots will be explained in more detail.

In order to centrifuge the subject's whole blood sample to separate serum and clots, the blood is placed in a test volume I! Although it can be put in
Inspection MIsII! The material is 1 flexible resin, sI
Hardenable camphor resin, modified natural camphor resin, glass etc. can all be used.

and Kl1 adhesion in the blood placed in the test container.

Preserve an inorganic substance *ljAdhesive inorganic substance is an inorganic substance used as an adsorbent, such as glass, silica, kaolin, celite, pennanite, etc.O water-resistant inorganic fine powder. Applicable.

It is preferable to use adsorbent inorganic substances having a particle size of sO- or less and an average particle size of 10 μm or less. And especially Blood JI! The adsorptive inorganic substance effective in reducing the time by -1 is silica, and the adsorbent inorganic material is effective for reducing the time by 9 minutes.

Such adsorptive inorganic substances act to promote the activation of the #I factor and promote the aggregation of the platelets when they come into contact with the particles. However, in order for adsorbent inorganic materials to effectively exert a one-liquid concentration promoting effect, K is linseed oil absorption, BI
It is determined that the ETjt barley area value and the round area value are within the range of one foot.

Linseed oil-absorbing fish and BIT ratio surface accumulation are non-adsorbent II.
I represents the surface area of mold powder, and the horizontal side of the mold is an adsorbent inorganic substance O! do! Since the degree of one-sided hole l[ and r14 are reached,
@The degree of pore OIM can be determined by the amount of curvature and specific surface area. The adsorptive inorganic material in the present invention has a flaxseed oil absorption amount of 20-40 m/10.9% B11Tk,
Those having a surface area value of 5ooo to 300(11) m/F are used.

Linseed oil absorption shows a self-sufficient value in accordance with Japanese Industrial Standards -1101, and absorbent inorganic substances 1~zsrtgatx
Place a drop of flax oil on a plate (approximately 100 ml) in the center of the casting sample, and each time mix thoroughly with a spatula until the sample suddenly becomes soft with a drop of linseed oil. Naomi sticks to the Gakusu board
The amount of linseed oil used up to the tm point is determined, and the 4 cycles of linseed oil for % sample 1009 are taken as the linseed oil absorbent.

111ETjtWheat area value is Brt+nauer-Km
This is a value obtained from the multimolecular adsorption theory proposed by Mett-Teller K, and the theoretical company %J1
) drunkenness-alof American Chemic
al 5ociety IJL.

309 (193g)ill,! 611 (1917) 4
1. &&% is explained in detail.

The BET specific area value is determined by calculating the amount of gas that will cover the wheat m in a monomolecular layer from the amount of gas adsorbed on the surface of the adsorbent inorganic material, the equilibrium pressure at that time, and the saturated vapor pressure of adsorbed gas O. A good value is calculated by calculating the average cross-sectional area of the adsorbed gas molecules by 1*, and 11JIl gas, oxygen gas, argon gas, methane gas, etc. are used as the adsorbed gas. If this method vc1 is used, the surface area value including the pores that cannot be added by one rilli by measuring the linseed oil absorption amount is #1.
It is added.

Blood desire #1iSIKII activates factor Xll, that is, the sensitization factor, which increases KFi ingredients ik! It is necessary that three substances, factor IXII, prekallikrein, and polymeric kininogen, form a complex and be adsorbed, and one or two of these substances can
1111#i is said to not be activated. By the way, when an adsorbent inorganic substance is used in the hope of promoting blood*ii, if the surface area is very large (1 to 4 h), the X
The adsorption rate of 1llfi, prekallikrein, and polymeric kininogen will increase, in other words,
The rate of complex formation among the three components necessary for activation of factor ll is reduced, and the blood picture-promoting effect is on the contrary diminished.

On the other hand, if the surface area of the sugar mineral is too small, Jll
The solid factor O1j@0 decreases, promoting and blocking hemocoagulation.
You won't be able to expect much. For this reason, the adsorbent inorganic material in the present invention has a high absorption amount of linseed oil! O~40gIt
/100f, BET ratio surface #S OOO-3000
0txl/fit) has a range l1IO1k direction and width.

In addition, the adsorbent inorganic specific resistance value in the present invention is lXl0
IOΩ・1 or less, more preferably tl 5 × 104
A material with a resistance of Ω・1 or less is used. The specific resistance value is the reciprocal of electrical conductivity, and is the value at room temperature.

When the wire comes into contact with a foreign object, proteins such as albumin, globulin, and various blood coagulation factors are immediately adsorbed to the surface of the foreign object, prior to the formation of isocoagulant, and the concentration of the IIO protein molecules increases. The changes have a variety of effects on the subsequent biochemical reactions, especially the enzyme reaction 6.
#The fixed factor 0 activation mechanism is greatly influenced by lI! Impairs solid function.

In addition, a large conformational change occurs, causing the mold to adhere to the purine and albumin O, resulting in an abnormally small and tl shape, and the phenomenon in which the precipitated fibrin sticks strongly to the adsorbent inorganic material. 7. Even if centrifugation is performed, blood clots and serum will not be separated, and the blood clots will not be able to form a blood clot and serum. .

Changes in the white matter o-con 7 Omeleon #i These occur as a result of various interactions between adsorbent inorganic substances and proteins, such as hydrophobic interactions, hydrogen bonding interactions, and electrostatic hook interactions. Among these, electrostatic hook interactions Concerning work 1@, comparison #I can be alleviated by using an adsorbent inorganic material with high exclusive electric properties. In other words, Kt
However, if the adsorptive inorganic substance is non-conductive, the response will be worse than if it is conductive, and *1klKlj The potential distribution of the adhering protein and the potential distribution of the adsorbing inorganic substance are not consistent with each other, and this causes local and non-uniform strain on the protein, resulting in contamination. f conversion 1
k-garu. *The fact that an adsorbent inorganic substance has conductivity 1 means that
The adsorbent inorganic substance in the present invention has a relatively high O has a resistance value of I x 10toΩ·α or less.

For the rounding of adsorptive inorganic substances present in tm liquid.

For example, an adsorbent inorganic substance may be immediately added to the axillary part of the test container, or the adsorbent inorganic substance may be added immediately after the adsorption inorganic substance is dispersed in the dispersion medium.
It may be added dropwise, or it may be added by adhering it to a carrier having a specific gravity of LO4 to LOIli.

The adsorbent inorganic substance Ot present in blood is 10g of blood.
l1li) sxio-・f to 1sXI@""'f is preferable, and if the amount is smaller than S
If there is a large amount of snow tx*, there is a risk of damaging the line inspection twi.

According to the method of the present invention, the adsorbable IlIl substance rapidly converts the Kf! 5 sexes, the time required for blood donation was significantly increased, and separation of blood serum and blood clots was carried out in 14 days. The problem of
i The yield of serum increases as a result of the sufficient contraction of the rice cake.

Father-in-law 11 As an adsorbent inorganic substance, the amount of linseed oil is 30s//1G
Of, BETJtjllli product is L 2 x lO't
d/f.

Specific resistance is 14!1X104Ω・α, average particle size is 5μm
Using fine silica powder, 1g of 10% by weight aqueous dispersion
I did it.

Next, prepare (a) a 10 dl 13 glass test container, (b) a 110-meter polyethylene test container, and (c) a 10 d polymethyl methacrylate test container S, and make a large test volume l. !Lim S- by 8d and immediately-
Add 6 Isf of water dropwise and leave at 20°C.

The time required for all the blood to stop flowing was measured as 9 o'clock and 1 llt blood #1 cycle. Blood clot li! Immediately after iI
Perform centrifugation at 100O11/min for 5 minutes, observe serum separation, and pipette the upper N serum into IIT.
x, the amount was taken as the serum yield・So0at wheat 1O%1
Is141t) lIIK Indication f@Example As an adsorbent inorganic substance, linseed oil absorption capacity is 30 d/l・O
f%BKTjt surface area is L! X104, specific resistance! 6X
A cylindrical polyethylene resin pellet with a diameter of 3 mm and a thickness of 1 MO was prepared using silica mold powder of 104Ω・1 and an average particle diameter of 2 mm (adjusted to a specific gravity of 9-0 by adding 9-5% of sulfuric acid). K* cloth and attached.

Next (a) 1 (11is for inspection made by Gatsuga 2S for LSF)
Polyethylene test container for l/- (c) l/-M
II damethyl methacrylate inspection mill tjII
When the dog was born, the dog was inspected and taken into custody one by one, and immediately silica mold powder was attached to it, and a polyethylene fence pellet TLF was added to it. Leave it at 61C.

It is shown in the column of Example 2 of 20 cases of one wave Jllll1 station, serum isolated mackerel, and Yuuki Jlk&l.

Comparative Example 1 (A) A test container made of glass for 10-, a test container made of polyethylene for (→10-), and (c) a test container 11I made of polymethyl methacrylate for 10- were prepared and a large scale calibration was carried out. Collect blood from 8 incisions into a container and leave it at 20°C.

Blood coagulation time in this case, -separation form 1, serum collection t-
It is shown in Comparative Example 10111 in Table 1.

Comparison 4 #12 As an adsorbent inorganic substance, linseed oil absorption amount is 280J/1G
Of, the BET specific surface area is 1 OX 10'eMf, the specific resistance is LOX 1013 Ω (1 m, and the same operation as in Example 2 was performed except for 9 using fine silica powder with an average particle size of about 7 μm. In this case blood # solidification time, serum separation, serum yield l
It is shown in the column of Comparative Example 2 of kl.

Comparative Example 3 Linseed oil absorption amount is 110J/1GO as adsorbent inorganic material
f%BIE Ding-Kanmu-product is 10 x 106d/l.

The same operation as in Example 2 was carried out except for using fine silica powder tP11 having a specific resistance of t9×1g4Ω·1 and an average particle size of topm.

In this case, the wire J1M1 time open, self-cleaning separation state l, and -clearing yield t*10 are shown in Comparative Example 3011.

Comparative Example 4 Absorption of linseed oil with adsorbent inorganic-2! 9-710@f
%BICT specific surface area# L o X 1 o4ad/f
.

Specific resistance #LIX101! Ω・1, average grain **tsp sea bream O
Carry out the same operations as in the Fi dormitory example except for using silica mold powder.

In this case, the % self-clearing yield after solidification and self-clarification is shown in Table 10 of Comparative Example 4.

Claims (1)

[Claims] L In the method of separating blood into serum and blood clots by subjecting it to centrifugation, the amount of linseed oil absorbed in the blood is 20 to 40s+f/10@f%BIET ratio i) i 50
@@no! 1GGo6j/f, adsorption I#, Il# with specific resistance of I x 1010 Ω・1 or less at room temperature
I! 1181-110 - A method for separating serum and blood clots, characterized in that J is present and t is present.