JPS58116422A - Antitumor agent - Google Patents

Abstract

PURPOSE:An antitumor agent, containing a live vaccine of a virus belonging to the genus Paramyxovirus as an active constituent, and capable of exhibiting the antitumor effect on mammals. CONSTITUTION:An antitumor agent containing a live vaccine of a virus belonging to the genus Paramyxovirus, e.g. parainfluenza 1, 2 or 3 or mumps virus, as a principal constituent. The mumps virus is particularly preferred. The live vaccine of the virus is obtained by the subculture and attenuation of the virus in a grown hen's egg, yalk sac or amniotic cavity, and the attenuated viral live vaccine is if desired subjected to the purification, germ-free filtration, etc. and in general orally, preferably parenterally, administered. In experiments on ddy mice, BDF1 mice, etc., the viral live vaccine is observed to have the antitumor effect on the Ehrlich's cancer and leukemia L1210 cancer.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to antitumor agents. More specifically, the present invention relates to an antitumor agent containing a live vaccine of a virus of the genus Balamyxovirus as a main ingredient.

Paramyxovirus (Paramyxovirus)
) is so named because of its morphological resemblance to 1 orthomyxovirus. The diameter of this virus is 1
It has a spherical shape of 50 to 600 nm, contains a helical nucleocap inside the engrape, and the nucleic acid has a molecular weight of 4 to 8.
This virus, which has a single domain of XlO and does not serve as a template for protein synthesis, often causes respiratory diseases in humans and animals.

The present inventors have demonstrated that a live vaccine for a virus of the genus Balamyxovirus can be used in mammals (humans, horses, mice,
They discovered that it has anti-II activity against rats, dogs, cows, etc., and completed the present invention.

This product consists of an anti-tumor agent whose main ingredient is a live vaccine of Varamyxovirus.

As the rose myxovirus used in the present invention, rose influenza 1! , types 2 and 3, Newcastle disease virus, mumps virus, measles virus, distemper virus, Sendai virus, etc.
Particularly preferred is mumps virus.

Live vaccines of such viruses are produced by procedures known per se, such as by subculturing the virus in embryonated chicken eggs, egg yolks, sheep fat, etc., and attenuating the virus.

The attenuated live virus vaccine may be subjected to treatments such as n-folding and sterilization filtration, if desired, in order to be used for medical purposes.

The anti-mm agent of the present invention is generally administered orally or preferably parenterally, and its dosage form is preferably a live vaccine, particularly a freeze-dried preparation.

Stabilizers such as albumin and gelatin may be added to the lyophilized preparation. In addition, lyophilized preparations are usually filled into ampoules and temporarily dissolved to be used as injections.
The filling per ampoule is usually 5 to 1100 II. Regarding the present invention, the dose of the live vaccine varies depending on symptoms, route of administration, body weight, etc., but is administered in accordance with the dose when administering the live vaccine for conventional purposes.

Next, the invention anti-#! Live vaccine anti-S, which is the main component of the antiseptic
An experimental example is shown to confirm this effect.

Experimental Example 1 Experimental animals were ddY mice (body weight approx. 2011cm/group 2).
0 mice) and BDF1 mice (body weight approximately 18.f, group of 20 mice) were used, and ddY mice were injected with 20 x IO cells of Ehrlich pigeon cells, and BDFl-f mice were injected with 2 x lθ'' of Leukomia L1210 cancer cells. Live vaccine of HVJ virus (Sendai virus) (the whole HVJ virus was used because it has selective susceptibility to mice) according to the administration method shown in 1.
0OOHA value based on Watori hemagglutination test]
At! The drug was administered intraperitoneally to mice, and the survival days of the mice were observed. The results were as shown in Table 1.

(Blank below) It has been confirmed that the HVJ virus live vaccine has an amazing anti-lung cancer effect.90 From these results, other live vaccines of the genus Varamyxovirus have an anti-vaginal effect. Live vaccines against mumps virus (human virus, which is highly susceptible to humans and corresponds to mouse IVJ virus) have been shown to have similar effects on human ulcers.

Practical IIA Example 1 A live vaccine of mumps virus (MLV) was adjusted to a concentration of 2×I O'p, f, u, /d in 100 m of physiological saline, and albumin 596 (w/man, gelatin O, s) was added to this. % (v/ma) amount was added and filtered for sterilization.◇This filtrate was dispensed and lyophilized.The obtained lyophilized live MLV vaccine was asxlfp, f, ile.
/) and 6.8XI even after storage for 1 month at -1θ℃
Op, f, u, /) activities were retained.

CP-f, ile (Plaque formin
g t * nit ): National Institute of Preventive Health Alumni Association
"Virus Experiments, Specifics" Maruzen (1967)) Example 2 HVJ virus live vaccine was added to albumin 5% (w/v)
) and adjusted to 60,000 HA/slj, followed by sterilization filtration. This filtrate was divided into portions and frozen at -80°C. The obtained frozen product retains 60,000 yen even after being stored for 5 years.
0HA/d ``t',!, -flJ understood, even in the case of 7'j, 60,000HA/d after 2 weeks when stored at 4℃
sl, ao, ooo~6°000 after 3-4 weeks
It was HA/d.

Procedural amendment (volunteer to the Commissioner of the Japan Patent Office1, Indication of the case 1982 Patent No. 1 No. 2114363, Person making the amendment Relationship to the case Patent applicant (1) Line 9 from the bottom of page 1 of the specification In the last line and in the 5th and 7th lines of page 2, the text ``paramyxo'' is corrected to ``paramyxo''.

(2) In the 8th line from the bottom of page 1 of the circular, the word ``orthomicun'' is corrected to ``orthomixo.''

(3) In the third line from the bottom of page 3 of the same book, the word "leudemia" has been corrected to "leukemia."

(4) In the same book, page 6, lines 15 and 16, "da" toraru is corrected to "-".

(5) On page 6, line 16 of the same book, there is a meeting called rlO'J "10
” is corrected.

Claims (1)

[Claims] An antitumor agent containing a live vaccine of a virus of the genus Paramyxovirus as an active ingredient.