JPS5797450A - Measuring method for enzyme immunity by competitive combination method of hormone using regenerable immune adsorption body - Google Patents

Measuring method for enzyme immunity by competitive combination method of hormone using regenerable immune adsorption body

Info

Publication number
JPS5797450A
JPS5797450A JP17262680A JP17262680A JPS5797450A JP S5797450 A JPS5797450 A JP S5797450A JP 17262680 A JP17262680 A JP 17262680A JP 17262680 A JP17262680 A JP 17262680A JP S5797450 A JPS5797450 A JP S5797450A
Authority
JP
Japan
Prior art keywords
hormone
enzyme
bond
antibody
combined
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP17262680A
Other languages
Japanese (ja)
Inventor
Kanefusa Kato
Akira Kosaka
Ryohei Yamamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AMANO SEIYAKU KK
Amano Enzyme Inc
Original Assignee
AMANO SEIYAKU KK
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AMANO SEIYAKU KK, Amano Pharmaceutical Co Ltd filed Critical AMANO SEIYAKU KK
Priority to JP17262680A priority Critical patent/JPS5797450A/en
Publication of JPS5797450A publication Critical patent/JPS5797450A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Abstract

PURPOSE:To improve measuring accuracy of an enzyme immunity of hormone and to measure automatically, by reusing an insoluble carrier utilizing an exchange reaction of S-S bond and SH radical and adding a hydrophobic protein and salts to measuring system. CONSTITUTION:Tagged hormone combined enzyme such as beta-galactosidase with a hormone, insulin, etc. is prepared. On the one hand, glutathione-2-pyridylsulfide is incorporated into an insoluble carrier such as ''Cephadex G25 '' and a bead or tube is prepared making S-S bond of said carrier and antiimmunogloblin. Said tagged hormone is added to 10mM phosphoric acid buffer solution of 7pH incorporated, for example, 0.3M NaCl, 1mM MgCl2, 0.5% gelatine, 0.1% cattle blood serum albumin, 0.1% NaN3 and 2mM N-ethylmaleiimid to human blood serum to be measured and said solution is let flowed to said tubular carrier and then, enzyme is combined. Next, the S-S bond is exchanged for a S-H radical by a reducing agent and combined body of enzyme-antibody-hormone is eluted and then, hormone is determined by measuring the activity of enzyme in an eluate. Inactive antibody is reused by making S-S bond of the antibody again.
JP17262680A 1980-12-09 1980-12-09 Measuring method for enzyme immunity by competitive combination method of hormone using regenerable immune adsorption body Pending JPS5797450A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP17262680A JPS5797450A (en) 1980-12-09 1980-12-09 Measuring method for enzyme immunity by competitive combination method of hormone using regenerable immune adsorption body

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP17262680A JPS5797450A (en) 1980-12-09 1980-12-09 Measuring method for enzyme immunity by competitive combination method of hormone using regenerable immune adsorption body

Publications (1)

Publication Number Publication Date
JPS5797450A true JPS5797450A (en) 1982-06-17

Family

ID=15945358

Family Applications (1)

Application Number Title Priority Date Filing Date
JP17262680A Pending JPS5797450A (en) 1980-12-09 1980-12-09 Measuring method for enzyme immunity by competitive combination method of hormone using regenerable immune adsorption body

Country Status (1)

Country Link
JP (1) JPS5797450A (en)

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