JPS5796257A - Kit for preparation of sample for inspection of urine callicrein - Google Patents

Kit for preparation of sample for inspection of urine callicrein

Info

Publication number
JPS5796257A
JPS5796257A JP55171773A JP17177380A JPS5796257A JP S5796257 A JPS5796257 A JP S5796257A JP 55171773 A JP55171773 A JP 55171773A JP 17177380 A JP17177380 A JP 17177380A JP S5796257 A JPS5796257 A JP S5796257A
Authority
JP
Japan
Prior art keywords
callicrein
sample
urine
column
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55171773A
Other languages
Japanese (ja)
Inventor
Shigehiro Morichi
Masaaki Yamada
Eiji Sako
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Tanabe Pharma Corp
Original Assignee
Green Cross Corp Japan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Green Cross Corp Japan filed Critical Green Cross Corp Japan
Priority to JP55171773A priority Critical patent/JPS5796257A/en
Publication of JPS5796257A publication Critical patent/JPS5796257A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96455—Kallikrein (3.4.21.34; 3.4.21.35)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To enable the quantity of callicrein to be specifically as well as highly sensitively measured, through preparation of a sample excellent in sensitivity by performing chromatography by the use of an adsorbent. CONSTITUTION:A sample solution is prepared by adding radioactive compound labeled callicrein to a sample and mixing them with each other and is charged in the upper part of a column (a) filled with silica gel equilibrated by a buffer of pH 7.0. Then, development is performed, and when there is no more supernatant, this is developed by a sample-developing buffer A, and 99% enzymes other than callicrein are adsorbed and removed. The solution having passed is developed by using a column (b) filled with fixing trypsin inhibitor in order to adsorb callicrein. After the column is washed with the sample-developing buffer A, elution is conducted by using a developing buffer B, and with the resultant eluate used as a samle the enzyme activity of urine callicrein is measured. Thereby, it is possible to highly sensitively measure an extremely small quantity of callicrein present in human urine.
JP55171773A 1980-12-05 1980-12-05 Kit for preparation of sample for inspection of urine callicrein Pending JPS5796257A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55171773A JPS5796257A (en) 1980-12-05 1980-12-05 Kit for preparation of sample for inspection of urine callicrein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55171773A JPS5796257A (en) 1980-12-05 1980-12-05 Kit for preparation of sample for inspection of urine callicrein

Publications (1)

Publication Number Publication Date
JPS5796257A true JPS5796257A (en) 1982-06-15

Family

ID=15929406

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55171773A Pending JPS5796257A (en) 1980-12-05 1980-12-05 Kit for preparation of sample for inspection of urine callicrein

Country Status (1)

Country Link
JP (1) JPS5796257A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6342468A (en) * 1986-08-08 1988-02-23 Res Dev Corp Of Japan Clinical inspection method based on kallikrein conjugated sugar chain structure

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6342468A (en) * 1986-08-08 1988-02-23 Res Dev Corp Of Japan Clinical inspection method based on kallikrein conjugated sugar chain structure

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