JPS5783286A - Preparation of enzyme to make protopectin soluble - Google Patents
Preparation of enzyme to make protopectin solubleInfo
- Publication number
- JPS5783286A JPS5783286A JP15985480A JP15985480A JPS5783286A JP S5783286 A JPS5783286 A JP S5783286A JP 15985480 A JP15985480 A JP 15985480A JP 15985480 A JP15985480 A JP 15985480A JP S5783286 A JPS5783286 A JP S5783286A
- Authority
- JP
- Japan
- Prior art keywords
- protopectin
- enzyme
- mixture
- soluble
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To separate an enzyme to solubilize protopectin form a mixture of starch and pectin and to purify it, by carrying out affinity chromatography using a gel obtained by insolubilizing the mixture as an adsorbent.
CONSTITUTION: A mixture comprising an enzyme to solubilize protopectin obtained by cultivating a fungus belonging to the genus Saccharomyces, Galactomyces, etc. is subjected to affinity chromatography using a gel prepared by insolubilizing a mixture of starch and pectin in a weight ratio of starch to pectin of (0.3:1)W(10:1) as an adsorbent, so that an enzyme to solubilize protopectin is separated and collected.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15985480A JPS5783286A (en) | 1980-11-13 | 1980-11-13 | Preparation of enzyme to make protopectin soluble |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15985480A JPS5783286A (en) | 1980-11-13 | 1980-11-13 | Preparation of enzyme to make protopectin soluble |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5783286A true JPS5783286A (en) | 1982-05-25 |
JPH0121956B2 JPH0121956B2 (en) | 1989-04-24 |
Family
ID=15702674
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP15985480A Granted JPS5783286A (en) | 1980-11-13 | 1980-11-13 | Preparation of enzyme to make protopectin soluble |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5783286A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5807727A (en) * | 1992-01-20 | 1998-09-15 | Japan Tobacco Inc. | Pectinase from Saccharomyces bayanus |
-
1980
- 1980-11-13 JP JP15985480A patent/JPS5783286A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5807727A (en) * | 1992-01-20 | 1998-09-15 | Japan Tobacco Inc. | Pectinase from Saccharomyces bayanus |
EP0868854A3 (en) * | 1992-01-20 | 1999-05-12 | Japan Tobacco Inc. | Low-molecular pectin, and food and drink which contain low-molecular pectin |
Also Published As
Publication number | Publication date |
---|---|
JPH0121956B2 (en) | 1989-04-24 |
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