JPS5783286A - Preparation of enzyme to make protopectin soluble - Google Patents

Preparation of enzyme to make protopectin soluble

Info

Publication number
JPS5783286A
JPS5783286A JP15985480A JP15985480A JPS5783286A JP S5783286 A JPS5783286 A JP S5783286A JP 15985480 A JP15985480 A JP 15985480A JP 15985480 A JP15985480 A JP 15985480A JP S5783286 A JPS5783286 A JP S5783286A
Authority
JP
Japan
Prior art keywords
protopectin
enzyme
mixture
soluble
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP15985480A
Other languages
Japanese (ja)
Other versions
JPH0121956B2 (en
Inventor
Takuo Sakai
Norihara Tomo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP15985480A priority Critical patent/JPS5783286A/en
Publication of JPS5783286A publication Critical patent/JPS5783286A/en
Publication of JPH0121956B2 publication Critical patent/JPH0121956B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE: To separate an enzyme to solubilize protopectin form a mixture of starch and pectin and to purify it, by carrying out affinity chromatography using a gel obtained by insolubilizing the mixture as an adsorbent.
CONSTITUTION: A mixture comprising an enzyme to solubilize protopectin obtained by cultivating a fungus belonging to the genus Saccharomyces, Galactomyces, etc. is subjected to affinity chromatography using a gel prepared by insolubilizing a mixture of starch and pectin in a weight ratio of starch to pectin of (0.3:1)W(10:1) as an adsorbent, so that an enzyme to solubilize protopectin is separated and collected.
COPYRIGHT: (C)1982,JPO&Japio
JP15985480A 1980-11-13 1980-11-13 Preparation of enzyme to make protopectin soluble Granted JPS5783286A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP15985480A JPS5783286A (en) 1980-11-13 1980-11-13 Preparation of enzyme to make protopectin soluble

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15985480A JPS5783286A (en) 1980-11-13 1980-11-13 Preparation of enzyme to make protopectin soluble

Publications (2)

Publication Number Publication Date
JPS5783286A true JPS5783286A (en) 1982-05-25
JPH0121956B2 JPH0121956B2 (en) 1989-04-24

Family

ID=15702674

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15985480A Granted JPS5783286A (en) 1980-11-13 1980-11-13 Preparation of enzyme to make protopectin soluble

Country Status (1)

Country Link
JP (1) JPS5783286A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807727A (en) * 1992-01-20 1998-09-15 Japan Tobacco Inc. Pectinase from Saccharomyces bayanus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807727A (en) * 1992-01-20 1998-09-15 Japan Tobacco Inc. Pectinase from Saccharomyces bayanus
EP0868854A3 (en) * 1992-01-20 1999-05-12 Japan Tobacco Inc. Low-molecular pectin, and food and drink which contain low-molecular pectin

Also Published As

Publication number Publication date
JPH0121956B2 (en) 1989-04-24

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