JPS5772919A - Buckwheat fluor extract and extracting method thereof - Google Patents

Buckwheat fluor extract and extracting method thereof

Info

Publication number
JPS5772919A
JPS5772919A JP55149597A JP14959780A JPS5772919A JP S5772919 A JPS5772919 A JP S5772919A JP 55149597 A JP55149597 A JP 55149597A JP 14959780 A JP14959780 A JP 14959780A JP S5772919 A JPS5772919 A JP S5772919A
Authority
JP
Japan
Prior art keywords
extract
buckwheat
buckwheat flour
resultnat
ion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55149597A
Other languages
Japanese (ja)
Inventor
Hiromasa Shioda
Shoei Eda
Takao Shinoda
Itsuo Nishioka
Akira Yagi
Yukiyoshi Yanagihara
Akio Maruyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kissei Pharmaceutical Co Ltd
Original Assignee
Kissei Pharmaceutical Co Ltd
Kissei Yakuhin Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kissei Pharmaceutical Co Ltd, Kissei Yakuhin Kogyo KK filed Critical Kissei Pharmaceutical Co Ltd
Priority to JP55149597A priority Critical patent/JPS5772919A/en
Publication of JPS5772919A publication Critical patent/JPS5772919A/en
Pending legal-status Critical Current

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

PURPOSE: To isolated and purify a haptenic substance useful as a hyposensitsation therapeutic agent for erethismic (hypersensitive) patients to buckwheat flour, by extracting a buckwheat flour with an aqeuou medium, dialyzing,the resultnat extract, and subjecting the dialyzate to the ion exchange chromatography, gel filtration, etc.
CONSTITUTION: A buckwheat flour is extracted witi an aqeous medium, and the resultnat extract is then separated and purified by the dialysis, ion exchange column chromatogrphy, gel filtrationand revers layer (phase) column chromatography. In the process, the measured vaue of the molecular weight according to the gel filtration method["Sephadex G50 " with a 1/30M phosphoric acid buffer solution (pH=6.5)]is about 1,600, and the substance is transferred to the cathodic side by the electrophoresis (carrier: filter paper No.51 manufactured by Toyo Roshi Kaisha, Ltd., pyridine-acetate bufrer solution, 600V, 2hr). The resultant buckwheat extract has the following amino acid composition: the weight ratio of aspartic acid> threonine: erine: glutamic acid: alaninie: valine: isoleucine: ornithine: lysine: arginine: cysteine=1:1:1:4:1:1:1:2:1:1:1, and the D-glucose is contaoned as a asccharide constituent.
COPYRIGHT: (C)1982,JPO&Japio
JP55149597A 1980-10-25 1980-10-25 Buckwheat fluor extract and extracting method thereof Pending JPS5772919A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55149597A JPS5772919A (en) 1980-10-25 1980-10-25 Buckwheat fluor extract and extracting method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55149597A JPS5772919A (en) 1980-10-25 1980-10-25 Buckwheat fluor extract and extracting method thereof

Publications (1)

Publication Number Publication Date
JPS5772919A true JPS5772919A (en) 1982-05-07

Family

ID=15478679

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55149597A Pending JPS5772919A (en) 1980-10-25 1980-10-25 Buckwheat fluor extract and extracting method thereof

Country Status (1)

Country Link
JP (1) JPS5772919A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2702147A1 (en) * 1993-03-05 1994-09-09 Celbert Sa Groupe Inhibitor of collagenase activity and cosmetic composition containing such an inhibitor.

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2702147A1 (en) * 1993-03-05 1994-09-09 Celbert Sa Groupe Inhibitor of collagenase activity and cosmetic composition containing such an inhibitor.
WO1994020541A1 (en) * 1993-03-05 1994-09-15 Groupe Celbert Sa Collagenase activity inhibitor and cosmetic composition containing same

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