JPS5767592A - Protein having reassociating properties and its preparation - Google Patents
Protein having reassociating properties and its preparationInfo
- Publication number
- JPS5767592A JPS5767592A JP55142324A JP14232480A JPS5767592A JP S5767592 A JPS5767592 A JP S5767592A JP 55142324 A JP55142324 A JP 55142324A JP 14232480 A JP14232480 A JP 14232480A JP S5767592 A JPS5767592 A JP S5767592A
- Authority
- JP
- Japan
- Prior art keywords
- substance
- insulin secretion
- bordetella
- protein
- iap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PURPOSE: To obtain the titled substance, by dialyzing an active substance of insulin secretion enhancement capable of producing Bordetella into a urea-containing phosphate buffer solution, followed by separation and purification.
CONSTITUTION: A bacterium belonging to the genus Bordetella (Bordetella pertussis, etc.) is cultivated and purified to give an active substance of insulin secretion enhancement (N-IAP), which is incubated wih a phosphate buffer solution, separated, purified, and an element substance is further associated to synthesize N-IAP. This substance itself does not exhibit an activity of insulin secretion enhancement, and is acidic protein having a molecular weight by SDS-gel electrophoresis of 28,000± 1,200 and an isoeletric point of 5.8. Amino acid composition and composition ratio (μM/100μM) of protein component: Asp 9.3±1.0, Thr 7.4±0.8, Ser 10.6±1.1, Glu 10.6±1.1, Gly 11.2±1.2, Ala 10.6±1.1, Gys/2 1.2±0.2, Val 6.7±0.7, Met 1.6±0.2, Llue 3.2±0.4, Ile 5.5±0.6, Tyr 4.6±0.5, Phe 3.5±0.4, Lys 2.2±0.3, His 1.7±0.2, Arg 5.9± 0.6, Pro 4.4±0.5.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55142324A JPS5767592A (en) | 1980-10-11 | 1980-10-11 | Protein having reassociating properties and its preparation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55142324A JPS5767592A (en) | 1980-10-11 | 1980-10-11 | Protein having reassociating properties and its preparation |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5767592A true JPS5767592A (en) | 1982-04-24 |
Family
ID=15312691
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55142324A Pending JPS5767592A (en) | 1980-10-11 | 1980-10-11 | Protein having reassociating properties and its preparation |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5767592A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988002754A1 (en) * | 1986-10-20 | 1988-04-21 | Trion Forskning- Och Utvecklings Aktiebolag | New peptides and applications thereof |
WO1988004665A1 (en) * | 1986-12-22 | 1988-06-30 | Trion Forskning- Och Utvecklings Aktiebolag | A new pertussis toxin derived polypeptide and applications thereof |
-
1980
- 1980-10-11 JP JP55142324A patent/JPS5767592A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988002754A1 (en) * | 1986-10-20 | 1988-04-21 | Trion Forskning- Och Utvecklings Aktiebolag | New peptides and applications thereof |
WO1988004665A1 (en) * | 1986-12-22 | 1988-06-30 | Trion Forskning- Och Utvecklings Aktiebolag | A new pertussis toxin derived polypeptide and applications thereof |
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