JPS5767592A - Protein having reassociating properties and its preparation - Google Patents

Protein having reassociating properties and its preparation

Info

Publication number
JPS5767592A
JPS5767592A JP55142324A JP14232480A JPS5767592A JP S5767592 A JPS5767592 A JP S5767592A JP 55142324 A JP55142324 A JP 55142324A JP 14232480 A JP14232480 A JP 14232480A JP S5767592 A JPS5767592 A JP S5767592A
Authority
JP
Japan
Prior art keywords
substance
insulin secretion
bordetella
protein
iap
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP55142324A
Other languages
Japanese (ja)
Inventor
Makoto Tamura
Toshi Murai
Kiyoshi Ito
Chikanori Tomioka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaken Pharmaceutical Co Ltd
Original Assignee
Kaken Pharmaceutical Co Ltd
Kakenyaku Kako KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaken Pharmaceutical Co Ltd, Kakenyaku Kako KK filed Critical Kaken Pharmaceutical Co Ltd
Priority to JP55142324A priority Critical patent/JPS5767592A/en
Publication of JPS5767592A publication Critical patent/JPS5767592A/en
Pending legal-status Critical Current

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Landscapes

  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE: To obtain the titled substance, by dialyzing an active substance of insulin secretion enhancement capable of producing Bordetella into a urea-containing phosphate buffer solution, followed by separation and purification.
CONSTITUTION: A bacterium belonging to the genus Bordetella (Bordetella pertussis, etc.) is cultivated and purified to give an active substance of insulin secretion enhancement (N-IAP), which is incubated wih a phosphate buffer solution, separated, purified, and an element substance is further associated to synthesize N-IAP. This substance itself does not exhibit an activity of insulin secretion enhancement, and is acidic protein having a molecular weight by SDS-gel electrophoresis of 28,000± 1,200 and an isoeletric point of 5.8. Amino acid composition and composition ratio (μM/100μM) of protein component: Asp 9.3±1.0, Thr 7.4±0.8, Ser 10.6±1.1, Glu 10.6±1.1, Gly 11.2±1.2, Ala 10.6±1.1, Gys/2 1.2±0.2, Val 6.7±0.7, Met 1.6±0.2, Llue 3.2±0.4, Ile 5.5±0.6, Tyr 4.6±0.5, Phe 3.5±0.4, Lys 2.2±0.3, His 1.7±0.2, Arg 5.9± 0.6, Pro 4.4±0.5.
COPYRIGHT: (C)1982,JPO&Japio
JP55142324A 1980-10-11 1980-10-11 Protein having reassociating properties and its preparation Pending JPS5767592A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55142324A JPS5767592A (en) 1980-10-11 1980-10-11 Protein having reassociating properties and its preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55142324A JPS5767592A (en) 1980-10-11 1980-10-11 Protein having reassociating properties and its preparation

Publications (1)

Publication Number Publication Date
JPS5767592A true JPS5767592A (en) 1982-04-24

Family

ID=15312691

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55142324A Pending JPS5767592A (en) 1980-10-11 1980-10-11 Protein having reassociating properties and its preparation

Country Status (1)

Country Link
JP (1) JPS5767592A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002754A1 (en) * 1986-10-20 1988-04-21 Trion Forskning- Och Utvecklings Aktiebolag New peptides and applications thereof
WO1988004665A1 (en) * 1986-12-22 1988-06-30 Trion Forskning- Och Utvecklings Aktiebolag A new pertussis toxin derived polypeptide and applications thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002754A1 (en) * 1986-10-20 1988-04-21 Trion Forskning- Och Utvecklings Aktiebolag New peptides and applications thereof
WO1988004665A1 (en) * 1986-12-22 1988-06-30 Trion Forskning- Och Utvecklings Aktiebolag A new pertussis toxin derived polypeptide and applications thereof

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