JPS5761946A - Sample preparation for endotoxin assay and kit thereof - Google Patents
Sample preparation for endotoxin assay and kit thereofInfo
- Publication number
- JPS5761946A JPS5761946A JP13694880A JP13694880A JPS5761946A JP S5761946 A JPS5761946 A JP S5761946A JP 13694880 A JP13694880 A JP 13694880A JP 13694880 A JP13694880 A JP 13694880A JP S5761946 A JPS5761946 A JP S5761946A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- gel
- filtration
- allyldextran
- methylenebisacrylamide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
Abstract
PURPOSE:To prepare a sample for endotoxin assay with high sensibility, by gel filtration of a plasma sample using a gel of allyldextran cross-linked with N,N'- methylenebisacrylamide. CONSTITUTION:A sample of plasma or blood product is subjected to gel filtration using a gel of cross-linked allyldextran with N,N'-methylenebisacrylamide to prepare a sample for endotoxin assay, followed by recovery of a fraction of the highest molecular weight. As a kit for sample preparation above mentioned, a set is usable, that is composed of a column for sample filtration, a solution contg. a high molecular colored materials for addition to the sample and a buffer solution for sample elution. In this case, the column for sample filtration is made up in such a way that a column of 4-9mm. diameter is filled up with 1.5-2.5ml of gel of cross-linked allyldextran with N,N'-methylenebisacrylamide which is allowed to be in equilibrium with a buffer solution of 7.0-7.4pH and sealed.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13694880A JPS5761946A (en) | 1980-09-30 | 1980-09-30 | Sample preparation for endotoxin assay and kit thereof |
AT81304506T ATE12145T1 (en) | 1980-09-30 | 1981-09-29 | METHOD FOR PREPARING A SAMPLE TO BE USED IN A PROCEDURE FOR DETERMINATION OF ENDOTOXINS AND KIT FOR PREPARING SUCH SAMPLE. |
DE8181304506T DE3169271D1 (en) | 1980-09-30 | 1981-09-29 | Method for preparing sample for use in endotoxin test and kit for preparing said sample |
EP19810304506 EP0049161B1 (en) | 1980-09-30 | 1981-09-29 | Method for preparing sample for use in endotoxin test and kit for preparing said sample |
DK430681A DK430681A (en) | 1980-09-30 | 1981-09-29 | PROCEDURE FOR PREPARING A TEST FOR ENDOTOXIN DETERMINATION AND EQUIPMENT FOR USE IN THE PROCEDURE |
US06/489,387 US4476093A (en) | 1980-09-30 | 1983-04-28 | Kit for preparing sample for use in endotoxin test |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP13694880A JPS5761946A (en) | 1980-09-30 | 1980-09-30 | Sample preparation for endotoxin assay and kit thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5761946A true JPS5761946A (en) | 1982-04-14 |
JPS6346379B2 JPS6346379B2 (en) | 1988-09-14 |
Family
ID=15187253
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP13694880A Granted JPS5761946A (en) | 1980-09-30 | 1980-09-30 | Sample preparation for endotoxin assay and kit thereof |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5761946A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59154661U (en) * | 1983-04-01 | 1984-10-17 | 三菱レイヨン株式会社 | Endotoxin detection measurement equipment |
-
1980
- 1980-09-30 JP JP13694880A patent/JPS5761946A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59154661U (en) * | 1983-04-01 | 1984-10-17 | 三菱レイヨン株式会社 | Endotoxin detection measurement equipment |
JPH023168Y2 (en) * | 1983-04-01 | 1990-01-25 |
Also Published As
Publication number | Publication date |
---|---|
JPS6346379B2 (en) | 1988-09-14 |
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