JPS5731621A - Separation of blood constituents - Google Patents

Separation of blood constituents

Info

Publication number
JPS5731621A
JPS5731621A JP9516581A JP9516581A JPS5731621A JP S5731621 A JPS5731621 A JP S5731621A JP 9516581 A JP9516581 A JP 9516581A JP 9516581 A JP9516581 A JP 9516581A JP S5731621 A JPS5731621 A JP S5731621A
Authority
JP
Japan
Prior art keywords
cocytotaxin
anaphylatoxin
separation
proteins
leucotaxine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9516581A
Other languages
Japanese (ja)
Inventor
Haa Buiizeraa Yozefu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Publication of JPS5731621A publication Critical patent/JPS5731621A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/16—Blood plasma; Blood serum
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum

Abstract

The invention relates to a process for producing and obtaining anaphylatoxin- and cocytotaxin-containing leucotaxine preparations and anaphylatoxin and cocytotaxin proteins in molecularly homogeneous, biologically active form from contact-activated mammalian serum by the following steps:- separation of the proteins from other serum constituents to obtain a serum protein concentrate fraction,- separation of a part of accompanying foreign blood proteins from anaphylatoxin and cocytotaxin present in the said protein concentrate fraction,- isolation of the leucotaxine preparation by chromatography on hydroxyapatite,- and optionally further purification and/or separation of the leucotaxine preparation into anaphylatoxin and cocytotaxin proteins by chromatographical methods.The invention is characterized in that, prior to chromatography on hydroxyapatite, in the above-mentioned process, a major fraction of accompanying foreign blood constituents is separated from the said serum protein concentrate fraction by fractional elution and/or precipitation with a water-soluble alcohol and/or at least one molecular sieve filtration.
JP9516581A 1980-06-19 1981-06-19 Separation of blood constituents Pending JPS5731621A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19803022914 DE3022914A1 (en) 1980-06-19 1980-06-19 METHOD FOR OBTAINING A LEUKOTAXIN PREPARATE CONTAINING ANAPHYLATOXIN AND COCYTOTAXIN, AND ANAPHYLATOXIN AND COCYTOTAXIN IN A MOLECULAR UNIFORM FORM

Publications (1)

Publication Number Publication Date
JPS5731621A true JPS5731621A (en) 1982-02-20

Family

ID=6104950

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9516581A Pending JPS5731621A (en) 1980-06-19 1981-06-19 Separation of blood constituents

Country Status (5)

Country Link
US (2) US4495096A (en)
EP (1) EP0042560B1 (en)
JP (1) JPS5731621A (en)
AT (1) ATE9906T1 (en)
DE (2) DE3022914A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61235752A (en) * 1985-04-11 1986-10-21 Asahi Optical Co Ltd Material, device and method for separating cell
JPS63284A (en) * 1986-03-22 1988-01-05 Toa Nenryo Kogyo Kk Cell segregating agent, production thereof, cell segregating device using said agent and method for removing immunosuppressive lymphocyte and/or immunosuppressive factor forming cell using said agent

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE940698L (en) * 1984-04-05 1985-10-05 Univ Missouri Vaccine and serum for endotoxin associated disease and method of preparing same as well as to methods of immunization and treatment of such disease and to a detoxified endotoxin and bacterial mutant
US5006259A (en) * 1988-03-21 1991-04-09 E. I. Du Pont De Nemours And Company Process and means for use of anaphylatoxin adsorbents

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2710293A (en) * 1953-10-30 1955-06-07 Olin Mathieson Blood fractionation

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61235752A (en) * 1985-04-11 1986-10-21 Asahi Optical Co Ltd Material, device and method for separating cell
JPH0513269B2 (en) * 1985-04-11 1993-02-22 Asahi Optical Co Ltd
JPS63284A (en) * 1986-03-22 1988-01-05 Toa Nenryo Kogyo Kk Cell segregating agent, production thereof, cell segregating device using said agent and method for removing immunosuppressive lymphocyte and/or immunosuppressive factor forming cell using said agent

Also Published As

Publication number Publication date
ATE9906T1 (en) 1984-11-15
US4610815A (en) 1986-09-09
US4495096A (en) 1985-01-22
DE3022914A1 (en) 1981-12-24
EP0042560B1 (en) 1984-10-17
DE3166700D1 (en) 1984-11-22
EP0042560A2 (en) 1981-12-30
EP0042560A3 (en) 1982-06-09

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