JPS572695A - Preparation of adenosine-5'-triphosphate with immobilized yeast - Google Patents

Preparation of adenosine-5'-triphosphate with immobilized yeast

Info

Publication number
JPS572695A
JPS572695A JP7682480A JP7682480A JPS572695A JP S572695 A JPS572695 A JP S572695A JP 7682480 A JP7682480 A JP 7682480A JP 7682480 A JP7682480 A JP 7682480A JP S572695 A JPS572695 A JP S572695A
Authority
JP
Japan
Prior art keywords
yeast
adenosine
glycolytic
phosphoric acid
immobilized yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7682480A
Other languages
Japanese (ja)
Other versions
JPS6336755B2 (en
Inventor
Ichiro Senhata
Joji Kato
Kosaku Murata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tanabe Seiyaku Co Ltd
Original Assignee
Tanabe Seiyaku Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tanabe Seiyaku Co Ltd filed Critical Tanabe Seiyaku Co Ltd
Priority to JP7682480A priority Critical patent/JPS572695A/en
Publication of JPS572695A publication Critical patent/JPS572695A/en
Publication of JPS6336755B2 publication Critical patent/JPS6336755B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE: To prepare the titled substance, by polymerizing acrylamide monomer in a suspension of a yeast fungal body having the glycolytic activity, reacting the resultant immobilized yeast with an ATP precursor, a glycolytic substrate, Mg ions and a phosphoric acid donor.
CONSTITUTION: A yeast fungal body having the glycolytic activity is cultivated, collected and suspended in water. Acrylamide monomer is then polymerized in the resultant aqueous suspension in the presence of a crosslinking agent, a polymerization accelerator, etc. to give an immobilized yeast. The resultant immobilized yeast is then brought into contact with an aqueous solution containing an adenosine- 5'-triphosphate precursor, e.g. adenosine, AMP or ADP, a glycolytic substrate, magnesium ions, e.g. magnesium chloride, a phosphoric acid donor, e.g. a phosphate or a buffer solution thereof. The phosphoric acid donor in 0.05W1mol concentration is added to the reaction slution, and preferably a compound, e.g. L- cysteine or dithiothreitol, is present in the reaction solution.
COPYRIGHT: (C)1982,JPO&Japio
JP7682480A 1980-06-06 1980-06-06 Preparation of adenosine-5'-triphosphate with immobilized yeast Granted JPS572695A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7682480A JPS572695A (en) 1980-06-06 1980-06-06 Preparation of adenosine-5'-triphosphate with immobilized yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7682480A JPS572695A (en) 1980-06-06 1980-06-06 Preparation of adenosine-5'-triphosphate with immobilized yeast

Publications (2)

Publication Number Publication Date
JPS572695A true JPS572695A (en) 1982-01-08
JPS6336755B2 JPS6336755B2 (en) 1988-07-21

Family

ID=13616420

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7682480A Granted JPS572695A (en) 1980-06-06 1980-06-06 Preparation of adenosine-5'-triphosphate with immobilized yeast

Country Status (1)

Country Link
JP (1) JPS572695A (en)

Also Published As

Publication number Publication date
JPS6336755B2 (en) 1988-07-21

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