JPS572682A - Washing method of cell - Google Patents
Washing method of cellInfo
- Publication number
- JPS572682A JPS572682A JP7545880A JP7545880A JPS572682A JP S572682 A JPS572682 A JP S572682A JP 7545880 A JP7545880 A JP 7545880A JP 7545880 A JP7545880 A JP 7545880A JP S572682 A JPS572682 A JP S572682A
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solution
- washing
- microcarrier surface
- nonproteinic
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE: To prevent the peeling of cells from a microcarrier surface in washing thereof, by washing the cells grown on the microcarrier surface with an aqueous solution of a salt containing a nonproteinic water-soluble high polymeric substance.
CONSTITUTION: Cells grown on a microcarrier surface are washed with an aqueous solution of a salt adjusted to a suitable osmotic pressure. In the process, a nonproteinic water-soluble high polymeric substance, e.g. dextran, methyl cellulose or polyvinyl pyrrolidone, in an amount in the range of 0.0001W1wt%, preferably 0.001W0.1wt%, is added to the aqueous solution.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7545880A JPS572682A (en) | 1980-06-06 | 1980-06-06 | Washing method of cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP7545880A JPS572682A (en) | 1980-06-06 | 1980-06-06 | Washing method of cell |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS572682A true JPS572682A (en) | 1982-01-08 |
JPS6313675B2 JPS6313675B2 (en) | 1988-03-26 |
Family
ID=13576862
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7545880A Granted JPS572682A (en) | 1980-06-06 | 1980-06-06 | Washing method of cell |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS572682A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6244176A (en) * | 1985-08-21 | 1987-02-26 | Toray Ind Inc | Method for passage of animal cell |
-
1980
- 1980-06-06 JP JP7545880A patent/JPS572682A/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6244176A (en) * | 1985-08-21 | 1987-02-26 | Toray Ind Inc | Method for passage of animal cell |
Also Published As
Publication number | Publication date |
---|---|
JPS6313675B2 (en) | 1988-03-26 |
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