JPS5726746A - Determining method of hemoglobin bound to glucose - Google Patents

Determining method of hemoglobin bound to glucose

Info

Publication number
JPS5726746A
JPS5726746A JP10224280A JP10224280A JPS5726746A JP S5726746 A JPS5726746 A JP S5726746A JP 10224280 A JP10224280 A JP 10224280A JP 10224280 A JP10224280 A JP 10224280A JP S5726746 A JPS5726746 A JP S5726746A
Authority
JP
Japan
Prior art keywords
glucose
hemoglobin
hemoglobin bound
eluate
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP10224280A
Other languages
Japanese (ja)
Other versions
JPS6148867B2 (en
Inventor
Fumio Kamiyama
Kazutoshi Yamazaki
Minoru Obara
Mitsuo Nagata
Kenichi Kawamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP10224280A priority Critical patent/JPS5726746A/en
Publication of JPS5726746A publication Critical patent/JPS5726746A/en
Publication of JPS6148867B2 publication Critical patent/JPS6148867B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To quantitate a hemoglobin bound to glucose by using of a liquid chromatographic filter consisting essentially of glucose(meth)acrylate as a fixed phase, and an eluate of water at 3.0-9.0pH. CONSTITUTION:To a 2l separable flask is added a mixture of 400ml of a 4wt% aqueous solution of polyvinylalcohol, 90g tetraethyleneglycol, 10g tetramethylolmethanetriacrylate and 1.5g benzoylperoxide, and further 100g toluene. Then, the mixture is stirred at 400r.p.m. for 10hr at 80 deg.C, and the reactant is cooled. After cooling, 5-20mum porous polymer is obtained and prepared for a column of chromatography. The chromatograpy is performed at 6pH by use of eluate of 0.1N phosphate buffer solution, and pH is changed to 7 immediately prior to the appearance of a big peak 5 for a standard hemoglobin to produce peaks 2-4 for a hemoglobin bound to glucose.
JP10224280A 1980-07-24 1980-07-24 Determining method of hemoglobin bound to glucose Granted JPS5726746A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP10224280A JPS5726746A (en) 1980-07-24 1980-07-24 Determining method of hemoglobin bound to glucose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10224280A JPS5726746A (en) 1980-07-24 1980-07-24 Determining method of hemoglobin bound to glucose

Publications (2)

Publication Number Publication Date
JPS5726746A true JPS5726746A (en) 1982-02-12
JPS6148867B2 JPS6148867B2 (en) 1986-10-27

Family

ID=14322146

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10224280A Granted JPS5726746A (en) 1980-07-24 1980-07-24 Determining method of hemoglobin bound to glucose

Country Status (1)

Country Link
JP (1) JPS5726746A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03164155A (en) * 1989-11-22 1991-07-16 Aichi Pref Gov Sterilization of food or packaging material with alternate or mixed treatment using ozone and alcohol or organic acid
JPH05500003A (en) * 1989-09-01 1993-01-14 マーズ インコーポレイテッド acid stabilized pasta

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05500003A (en) * 1989-09-01 1993-01-14 マーズ インコーポレイテッド acid stabilized pasta
JPH03164155A (en) * 1989-11-22 1991-07-16 Aichi Pref Gov Sterilization of food or packaging material with alternate or mixed treatment using ozone and alcohol or organic acid

Also Published As

Publication number Publication date
JPS6148867B2 (en) 1986-10-27

Similar Documents

Publication Publication Date Title
EP0390577A3 (en) Purification and preparation processes for methyl methacrylate
OA06118A (en) New (pyrimidyloxy) phenoxyalkanecarboxylic acids, their preparation process and their application as herbicides.
Bygdeman et al. Isolation and identification of prostaglandins from ram seminal plasma
JPS5726746A (en) Determining method of hemoglobin bound to glucose
FR2416205A2 (en) IMPROVEMENT OF THE WET GYPSUM / PLASTER MIX COMPACTION PROCESS
Phillips The N-terminal groups of salmine
Jaan et al. 2‐Amino‐2, 6‐Dideoxy‐L‐Mannose (L‐Rhamnosamine) Isolated from the Lipopolysaccharide of Escherichia coli 03: K2ab (L): H2
EA199700097A1 (en) APPLICATION β-D-GALAKTOPIRANOSYL-D-XYLOSE FOR OBTAINING COMPOSITIONS AND SOLUTIONS INTENDED FOR THE DETERMINATION OF INTESTINAL LACTAGE AND METHOD FOR THEIR RECOVERY
JPS529757A (en) Clamping method of bolt and its washer
RU95112503A (en) Method of isolation of preliminary taken hemoglobin and the purified hemoglobin preparation
ES447851A1 (en) Process for the extraction of components having anticoagulant activity {37 in vivo{38 {0 from snake venoms and products obtained
JPS6486062A (en) Solution for adjusting behavior of ion exchange chromatography column of hplc apparatus
GR3029384T3 (en) Method for the isolation and purification of nucleotid-activated sugars from biological sources
Corti et al. Thin-layer chromatography and densitometry in drug assay: comparison of methods for monitoring valproic acid in plasma
JPS53124682A (en) Preparation of complex of enzyme and bioactive substance and its use
JPS5420195A (en) Preparation of guanosine-5'-monophosphoric acid
AU530657B2 (en) Apoglucose oxidase
Høye Purification of Insulin labelled with Iodine-131
SU1062608A1 (en) Method of determination of gystamin in wine
JPS5235457A (en) Controlling method for addition of organic carbon in biological denitr ogen method
JPS6410971A (en) Production of antioxidant substance
JPS5398938A (en) Novel process for their preparation of insecticides
ITO et al. On the determination of boric acid in frozen peeled shrimp
JPS53135918A (en) Preparation of 3,3-dimethyl-1-hexen-5-one
KR870002850A (en) How to Extract Atractylone from Creation