JPS57135361A - Latex reagent and measuring method of latex coagulation reaction - Google Patents
Latex reagent and measuring method of latex coagulation reactionInfo
- Publication number
- JPS57135361A JPS57135361A JP2054781A JP2054781A JPS57135361A JP S57135361 A JPS57135361 A JP S57135361A JP 2054781 A JP2054781 A JP 2054781A JP 2054781 A JP2054781 A JP 2054781A JP S57135361 A JPS57135361 A JP S57135361A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- latex
- reagent
- styrene
- hbs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Abstract
PURPOSE:To perform optical measurement for an antigen-antibody reaction with high sensitivity without occurring nonspecific coagulation by using a reagent of which an antibody is sensitized against a latex of which styrene and styrene sulfonic acid salts are polymerized without using an emulsifying agent under the existence of an ultrafine silicic anhydride. CONSTITUTION:Styrene and styrene sulfonic acid salts are copolymerized with a K2S2O8 used as an initiator under the existence of an ultrafine silicic anhydride having 5-50mmu particle size. Thereafter, said reactants are heated in pH value 8-11.5 at about 70 deg.C to obtain a latex having about 0.45mu average particle size. And, for example, an HBs antibody is combined with the latex. Then, said combined antibody is treated with a serum exclusive of an anti-human protein antibody from an antiserum of a guninea pig immunized against the HBs antibody to obtain a latex reagent. Further, after a patient serum contg. an HBs anti-antibody is reacted with the latex reagent, the absorbancy is measured. Thereby, said method can measure up to about 10<-8>g/sec antibody quantities and is highly sensitive as high as 1,000 times conventional cases.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2054781A JPS57135361A (en) | 1981-02-13 | 1981-02-13 | Latex reagent and measuring method of latex coagulation reaction |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2054781A JPS57135361A (en) | 1981-02-13 | 1981-02-13 | Latex reagent and measuring method of latex coagulation reaction |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57135361A true JPS57135361A (en) | 1982-08-20 |
JPS6352707B2 JPS6352707B2 (en) | 1988-10-19 |
Family
ID=12030171
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2054781A Granted JPS57135361A (en) | 1981-02-13 | 1981-02-13 | Latex reagent and measuring method of latex coagulation reaction |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57135361A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1416277A1 (en) * | 2001-07-02 | 2004-05-06 | Sekisui Chemical Co., Ltd. | Carrier particle latex for assay reagent and assay reagent |
-
1981
- 1981-02-13 JP JP2054781A patent/JPS57135361A/en active Granted
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1416277A1 (en) * | 2001-07-02 | 2004-05-06 | Sekisui Chemical Co., Ltd. | Carrier particle latex for assay reagent and assay reagent |
EP1416277A4 (en) * | 2001-07-02 | 2005-11-16 | Sekisui Chemical Co Ltd | Carrier particle latex for assay reagent and assay reagent |
AU2002313319B2 (en) * | 2001-07-02 | 2007-05-10 | Sekisui Medical Co., Ltd. | Carrier particle latex for assay reagent and assay reagent |
EP2023141A3 (en) * | 2001-07-02 | 2010-01-06 | Sekisui Medical Co., Ltd. | Carrier particle latex for assay reagent, and assay reagent |
Also Published As
Publication number | Publication date |
---|---|
JPS6352707B2 (en) | 1988-10-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4828981A (en) | Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents | |
AU611971B2 (en) | Positive step immunoassay | |
AU616484B2 (en) | Method for determination of a polyvalent substance using an immunoaggregate | |
US4480042A (en) | Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays | |
US4241176A (en) | Magnetic gel suitable to immunoenzymatic determinations | |
CN107894509A (en) | A kind of method for improving latex immunoturbidimetry antigen excess and the range of linearity | |
JPS604938B2 (en) | Immunological reagents and their use | |
JPS605899B2 (en) | Immunochemical quantitative method | |
CN109633170A (en) | A kind of Epstein-Barr virus shell antigen I gA antibody assay kit | |
Cuilliere et al. | Microparticle-enhanced nephelometric immunoassay (Nephelia) for immunoglobulins G, A, and M | |
JPS57135361A (en) | Latex reagent and measuring method of latex coagulation reaction | |
ES484166A1 (en) | Method and reagent for the detection and the determination of complement-binding antibodies. | |
ES2125982T3 (en) | TURBIDIMETRIC IMMUNOLOGICAL TEST OF INITIAL SPEED. | |
Stevenson et al. | Evaluation and clinical application of a two-site immunoradiometric assay for alpha-1-foetoprotein using readily available reagents | |
JPS57102196A (en) | Measuring method of superoxide dismutase (sod) | |
JPH01301165A (en) | Immunoassay | |
JPS61280568A (en) | Method for measuring components in bodily fluids | |
JPS5757260A (en) | Manufacture of latex reagent | |
JPS56129862A (en) | Production of diagnosis latex reagent | |
AU602170B2 (en) | A method for determining the presence of substances of diagnostic relevance, in particular antibodies or antigens, by the ELISA method with photometric evaluation | |
Burrells et al. | The quantitative estimation of antibody to Pasteurella haemolytica in sheep sera using a micro-enzyme-linked immunosorbent assay (ELISA) | |
PrIce et al. | Kinetic immunoturbidimetry of human choriomammotropin in serum. | |
CN208766185U (en) | The quick detection kit of Autoimmune neuropathies systemic disease | |
JP2534067B2 (en) | Quantitative method for C-reactive protein | |
Leek et al. | Particle counting immunoassay (PACIA) V-its application to the determination of human placental lactogen |