JPS57128839A - Bioelectrochemical sensor and its production - Google Patents

Bioelectrochemical sensor and its production

Info

Publication number
JPS57128839A
JPS57128839A JP56014130A JP1413081A JPS57128839A JP S57128839 A JPS57128839 A JP S57128839A JP 56014130 A JP56014130 A JP 56014130A JP 1413081 A JP1413081 A JP 1413081A JP S57128839 A JPS57128839 A JP S57128839A
Authority
JP
Japan
Prior art keywords
electrode
soln
ice blocks
microbes
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56014130A
Other languages
Japanese (ja)
Other versions
JPS645251B2 (en
Inventor
Masaru Uehara
Akitaka Uchida
Akihiro Sakimae
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Rayon Co Ltd
Original Assignee
Mitsubishi Rayon Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Rayon Co Ltd filed Critical Mitsubishi Rayon Co Ltd
Priority to JP56014130A priority Critical patent/JPS57128839A/en
Publication of JPS57128839A publication Critical patent/JPS57128839A/en
Publication of JPS645251B2 publication Critical patent/JPS645251B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Abstract

PURPOSE:To obtain a sensor of superior activity, durability, etc. by covering the surface of an electrode with a mixture of ice blocks contg. enzyme or microbes and an org. solvent wherein a water-insoluble high polymer is dissolved, and removing the solvent thereby enclosing the enzyme or microbes into the high polymer matrix. CONSTITUTION:The ice blocks obtained by such a method of blowing water contg. enzyme or microbes such as mold, germs or the like into hexane of -70 deg.C and forming micro-ice blocks are filtered and are slurried in CH2Cl2. A CH2Cl2 soln. or the like wherein a water-insoluble high polymer such as cellulose acetate and which is cooled to <=0 deg.C is separately prepd. and said slurry is added into said soln., whereby a coating soln. is prepd. Said coating soln. is coated on the exposed part of an electrode 1 in the leading end part from the coated part of the insulator 4 of a barlike electrode (platinum wire or the like) 1, and the coating film is allowed to solidify in a cooling toluene bath. Next, the toluene is extracted away with an n-hexane, after which the ice blocks are thawed whereby the electrode coated with the cellulose acetate film of about 100mu thickness is obtained. In this way, the sensor virtually free from degradation in activity for a long period of time is obtained.
JP56014130A 1981-02-02 1981-02-02 Bioelectrochemical sensor and its production Granted JPS57128839A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56014130A JPS57128839A (en) 1981-02-02 1981-02-02 Bioelectrochemical sensor and its production

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56014130A JPS57128839A (en) 1981-02-02 1981-02-02 Bioelectrochemical sensor and its production

Publications (2)

Publication Number Publication Date
JPS57128839A true JPS57128839A (en) 1982-08-10
JPS645251B2 JPS645251B2 (en) 1989-01-30

Family

ID=11852545

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56014130A Granted JPS57128839A (en) 1981-02-02 1981-02-02 Bioelectrochemical sensor and its production

Country Status (1)

Country Link
JP (1) JPS57128839A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6428555A (en) * 1987-07-24 1989-01-31 Terumo Corp Enzyme sensor
WO1996026433A1 (en) * 1995-02-22 1996-08-29 Akebono Brake Industry Co., Ltd. Microbial electrode and microbial sensor

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0330179A (en) * 1989-06-27 1991-02-08 Nec Gumma Ltd Floppy disk device

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50137788A (en) * 1974-04-22 1975-11-01
JPS5598347A (en) * 1979-01-20 1980-07-26 Hitachi Ltd Enzyme electrode

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50137788A (en) * 1974-04-22 1975-11-01
JPS5598347A (en) * 1979-01-20 1980-07-26 Hitachi Ltd Enzyme electrode

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6428555A (en) * 1987-07-24 1989-01-31 Terumo Corp Enzyme sensor
WO1996026433A1 (en) * 1995-02-22 1996-08-29 Akebono Brake Industry Co., Ltd. Microbial electrode and microbial sensor

Also Published As

Publication number Publication date
JPS645251B2 (en) 1989-01-30

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