JPS57103056A - Reagent for measuring direct bilirubin - Google Patents

Reagent for measuring direct bilirubin

Info

Publication number
JPS57103056A
JPS57103056A JP18105180A JP18105180A JPS57103056A JP S57103056 A JPS57103056 A JP S57103056A JP 18105180 A JP18105180 A JP 18105180A JP 18105180 A JP18105180 A JP 18105180A JP S57103056 A JPS57103056 A JP S57103056A
Authority
JP
Japan
Prior art keywords
bilirubin
reagent
acid
direct
affected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP18105180A
Other languages
Japanese (ja)
Other versions
JPS6322262B2 (en
Inventor
Noboru Mitsuhida
Yoshitaka Nakagiri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP18105180A priority Critical patent/JPS57103056A/en
Publication of JPS57103056A publication Critical patent/JPS57103056A/en
Publication of JPS6322262B2 publication Critical patent/JPS6322262B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/728—Bilirubin; including biliverdin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To measure direct bilirubin involved in body fluids accurately without being affected by indirect bilirubin, by incorporating stabilized diazonium salt in a range of specified concentration together with hydrochloric acid in a reagent. CONSTITUTION:An aqueous solution containing 0.05-1.0millimol/l stabilized diazonium salt such as p-sulfobenzenediazonium-1,5-naphthalenedisulfonic acid salt (NDDS) and containing hydrochloric acid in the range of 0.01-0.1N is used for a reagent for measuring direct bilirubin (ester combination product of propionic acid of side chain of free bilirubin and glucuronic acid). The quantity of direct biliburin is measured accurately by this reagent not affected by the concentration of indirect bilirubin (free bilirubin) in body fluids (blood serum). Thus, it is used for the decision and medical treatment of various jaundice or a hemolytic disease of an unborn baby.
JP18105180A 1980-12-19 1980-12-19 Reagent for measuring direct bilirubin Granted JPS57103056A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP18105180A JPS57103056A (en) 1980-12-19 1980-12-19 Reagent for measuring direct bilirubin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18105180A JPS57103056A (en) 1980-12-19 1980-12-19 Reagent for measuring direct bilirubin

Publications (2)

Publication Number Publication Date
JPS57103056A true JPS57103056A (en) 1982-06-26
JPS6322262B2 JPS6322262B2 (en) 1988-05-11

Family

ID=16093910

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18105180A Granted JPS57103056A (en) 1980-12-19 1980-12-19 Reagent for measuring direct bilirubin

Country Status (1)

Country Link
JP (1) JPS57103056A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5923253A (en) * 1982-07-07 1984-02-06 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Direct type bilirubin and method and reagent of measuring total bilirubin

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4929475A (en) * 1972-07-19 1974-03-15
JPS5412840A (en) * 1977-06-30 1979-01-30 Canon Inc Measuring method for surface potential of electrophotographic photoreceptor by time series
JPS5544992A (en) * 1978-09-22 1980-03-29 Miles Lab Apparatus for and method of detecting bilirubin in serum and method of producing same apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4929475A (en) * 1972-07-19 1974-03-15
JPS5412840A (en) * 1977-06-30 1979-01-30 Canon Inc Measuring method for surface potential of electrophotographic photoreceptor by time series
JPS5544992A (en) * 1978-09-22 1980-03-29 Miles Lab Apparatus for and method of detecting bilirubin in serum and method of producing same apparatus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5923253A (en) * 1982-07-07 1984-02-06 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Direct type bilirubin and method and reagent of measuring total bilirubin
JPH0324629B2 (en) * 1982-07-07 1991-04-03 Boehringer Mannheim Gmbh

Also Published As

Publication number Publication date
JPS6322262B2 (en) 1988-05-11

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