JPS5698658A - Method of simple fractional determination of glucosidated hemoglobin in blood - Google Patents

Method of simple fractional determination of glucosidated hemoglobin in blood

Info

Publication number
JPS5698658A
JPS5698658A JP189480A JP189480A JPS5698658A JP S5698658 A JPS5698658 A JP S5698658A JP 189480 A JP189480 A JP 189480A JP 189480 A JP189480 A JP 189480A JP S5698658 A JPS5698658 A JP S5698658A
Authority
JP
Japan
Prior art keywords
hba
buffer solution
hemoglobin
solution
glucosidated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP189480A
Other languages
Japanese (ja)
Inventor
Shoji Maruyama
Masami Kenmotsu
Toshisada Ishido
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Original Assignee
SOGO SEIBUTSU IGAKU KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SOGO SEIBUTSU IGAKU KENKYUSHO KK filed Critical SOGO SEIBUTSU IGAKU KENKYUSHO KK
Priority to JP189480A priority Critical patent/JPS5698658A/en
Publication of JPS5698658A publication Critical patent/JPS5698658A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PURPOSE: To perform a fractional determination of hemoglobin (HbA1) in a blood simply by eluting a glucosidated hemoglobin (HbA1) in order of the fraction, HbA1(a+b), HbA1C.
CONSTITUTION: A column 22 packed with a weakly acidic cation exchange resin 20 and equilibrated with anionic buffer solution 21, is used. After flowing out the buffer solution 21, a hemolytic reagent (solution A) is added into the column. Then, the HbA1(a+b) is eluted with the first eluant 23 consisting of anionic buffer solution (a buffer solution containing a phosphate and a basic salt and having 0.03W 0.07M of ionic concentration) and an eluate (solution B) is obtained. Further, the HbA1C is eluted with the second eluant 24 consisting of anionic buffer solution (pH6.4W7.7 and 0.08W0.18M of cationic concentration) and an eluate (solution C) is obtained. Then, the each absorbancy of said solutions, A, B and C is measured and each concentration of HbA1(a+b), HbA1C is calculated using formulae I and II.
COPYRIGHT: (C)1981,JPO&Japio
JP189480A 1980-01-11 1980-01-11 Method of simple fractional determination of glucosidated hemoglobin in blood Pending JPS5698658A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP189480A JPS5698658A (en) 1980-01-11 1980-01-11 Method of simple fractional determination of glucosidated hemoglobin in blood

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP189480A JPS5698658A (en) 1980-01-11 1980-01-11 Method of simple fractional determination of glucosidated hemoglobin in blood

Publications (1)

Publication Number Publication Date
JPS5698658A true JPS5698658A (en) 1981-08-08

Family

ID=11514284

Family Applications (1)

Application Number Title Priority Date Filing Date
JP189480A Pending JPS5698658A (en) 1980-01-11 1980-01-11 Method of simple fractional determination of glucosidated hemoglobin in blood

Country Status (1)

Country Link
JP (1) JPS5698658A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58191968A (en) * 1982-04-26 1983-11-09 バイオ−ラツド・ラボラトリ−ズ・インコ−ポレイテツド Method of separating hemoglobin a1c
JPS58201065A (en) * 1982-05-20 1983-11-22 マイルス・イタリア−ナ・ソシエタ・ペル・アチオ−ニ Method of measuring hemoglobin glycoside and testing instrument for its measurement
US4810391A (en) * 1987-11-06 1989-03-07 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture
JPH01199166A (en) * 1988-02-04 1989-08-10 Nippon Chemiphar Co Ltd Method for simple and precise differential measurement of saccharified hemoglobin in blood
US4980058A (en) * 1987-11-06 1990-12-25 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture
CN104865326A (en) * 2015-05-28 2015-08-26 江苏奥迪康医学科技有限公司 Three-gradient elution analysis method of glycosylated hemoglobin analyzer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5499496A (en) * 1977-12-02 1979-08-06 Isolab Inc Method of deciding diagnosis index of blood sugar condition and its device

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5499496A (en) * 1977-12-02 1979-08-06 Isolab Inc Method of deciding diagnosis index of blood sugar condition and its device

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58191968A (en) * 1982-04-26 1983-11-09 バイオ−ラツド・ラボラトリ−ズ・インコ−ポレイテツド Method of separating hemoglobin a1c
JPS58201065A (en) * 1982-05-20 1983-11-22 マイルス・イタリア−ナ・ソシエタ・ペル・アチオ−ニ Method of measuring hemoglobin glycoside and testing instrument for its measurement
US4810391A (en) * 1987-11-06 1989-03-07 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture
US4980058A (en) * 1987-11-06 1990-12-25 Bio-Rad Laboratories, Inc. Separation of hemoglobin A2 from hemoglobin mixture
JPH01199166A (en) * 1988-02-04 1989-08-10 Nippon Chemiphar Co Ltd Method for simple and precise differential measurement of saccharified hemoglobin in blood
CN104865326A (en) * 2015-05-28 2015-08-26 江苏奥迪康医学科技有限公司 Three-gradient elution analysis method of glycosylated hemoglobin analyzer

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