JPS56501453A - - Google Patents

Info

Publication number
JPS56501453A
JPS56501453A JP50013880A JP50013880A JPS56501453A JP S56501453 A JPS56501453 A JP S56501453A JP 50013880 A JP50013880 A JP 50013880A JP 50013880 A JP50013880 A JP 50013880A JP S56501453 A JPS56501453 A JP S56501453A
Authority
JP
Japan
Prior art keywords
enzyme mixture
protein
units
solution
activity per
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP50013880A
Other languages
Japanese (ja)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/091,041 external-priority patent/US4286064A/en
Application filed filed Critical
Publication of JPS56501453A publication Critical patent/JPS56501453A/ja
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/44—Oxidoreductases (1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4873—Cysteine endopeptidases (3.4.22), e.g. stem bromelain, papain, ficin, cathepsin H
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/28—Rubbing or scrubbing compositions; Peeling or abrasive compositions; Containing exfoliants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A process for isolating an enzyme mixture useful in the treatment of devitalized tissue from crude bromelain. The process comprises the steps of (1) suspending the crude bromelain in a weakly basic buffer, preferably sodium borate buffer, to selectively dissolve the active enzyme mixture; (2) separating the undissolved solids from the solution; and (3) removing small molecules having a molecular weight of 10,000 or less from the solution. The product exhibits 15 to 35 units of protease activity per milligram of protein in the presence of cysteine and from 0.5 to 5 units of collagenase activity per milligram of protein. The enzyme mixture is dissolved in an inert solvent carrier containing at least 60 percent water prior to application to the devitalized tissue.
JP50013880A 1979-11-05 1980-10-24 Pending JPS56501453A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9104079A 1979-11-05 1979-11-05
US06/091,041 US4286064A (en) 1979-11-05 1979-11-05 Process for isolating active debriding agent from bromelain

Publications (1)

Publication Number Publication Date
JPS56501453A true JPS56501453A (en) 1981-10-08

Family

ID=26783198

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50013880A Pending JPS56501453A (en) 1979-11-05 1980-10-24

Country Status (5)

Country Link
EP (1) EP0039726A4 (en)
JP (1) JPS56501453A (en)
AU (1) AU543965B2 (en)
CA (1) CA1156931A (en)
WO (1) WO1981001242A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8724728D0 (en) * 1987-10-22 1987-11-25 Genzyme Corp Cysteine proteinase
IL165334A0 (en) 2004-11-22 2006-01-15 Mediwound Ltd Debriding composition from bromelain and methods of producing same
ITMI20052076A1 (en) 2005-10-31 2007-05-01 Stel S N C Dell Ing Ermete Riva & C EQUIPMENT FOR ENDOTONIC TREATMENT BY CIRCULATION OF ENZYMATIC SOLUTIONS IN THE PULPAR CHAMBER AND IN THE ROOT CHANNELS
US11382958B2 (en) 2016-01-31 2022-07-12 Mediwound Ltd. Debriding composition for treating wounds
WO2017183018A1 (en) * 2016-04-18 2017-10-26 Mediwound Ltd Methods of debridement of chronic wounds
CN113679828A (en) * 2021-08-25 2021-11-23 佛山市第一人民医院（中山大学附属佛山医院） Wound cleaning fluid and preparation method and application thereof
CN114107265B (en) * 2021-11-05 2023-12-01 罗钰玲 Process for extracting bromelain

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3446706A (en) * 1966-01-27 1969-05-27 Jack F Beuk Process of producing plant proteases
US3446626A (en) * 1966-08-25 1969-05-27 John K Mcanelly Method for preparing a bromelain solution for ante-mortem injection
US3442764A (en) * 1966-09-12 1969-05-06 Swift & Co Process of purifying bromelain
US4197291A (en) * 1974-01-08 1980-04-08 Gerold K. V. Klein Hydrolytic enzyme material

Also Published As

Publication number Publication date
EP0039726A1 (en) 1981-11-18
AU6573480A (en) 1981-05-22
EP0039726A4 (en) 1982-09-27
CA1156931A (en) 1983-11-15
AU543965B2 (en) 1985-05-09
WO1981001242A1 (en) 1981-05-14

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