JPS5635051A - Measuring method of enzyme activity - Google Patents
Measuring method of enzyme activityInfo
- Publication number
- JPS5635051A JPS5635051A JP11081779A JP11081779A JPS5635051A JP S5635051 A JPS5635051 A JP S5635051A JP 11081779 A JP11081779 A JP 11081779A JP 11081779 A JP11081779 A JP 11081779A JP S5635051 A JPS5635051 A JP S5635051A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- merdola
- blue
- nadh
- nad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To increase the accuracy of measurement at low concentration solution for quantitative analysis of enzyme activity, by using the merdola blue as electron carrier.
CONSTITUTION: In the buffer liquid tank 1, for example, NAD in 1μM, lactic acid of 50μM, merdola blue of 10μM, and phosphric acid buffer solution pH8.0 of 0.1M, are put in. Further, the sample LDH(enzyme for dehydrogen lactic acid) is injected from the intake 2, and the buffer solution and sample are fed to the electrode 3 with the pump 4. To the electrode 3 as active electrode and to contrast electrode, platinum and saturated caromel electrode are used respectively. In this case, corresponding to LDH activity, NAD becomes NADH. The NADH is changed into NAD by using the oxygen type merdola blue, and the reduction type merdola blue changed corresponding to NADH is electrochemically oxided by using the electrode 3, and the current of oxidation is measured via the current voltage converter 6 and the voltmeter 7 to quantitatively analyze the lactic acid dehydrogen enzyme.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11081779A JPS5635051A (en) | 1979-08-29 | 1979-08-29 | Measuring method of enzyme activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11081779A JPS5635051A (en) | 1979-08-29 | 1979-08-29 | Measuring method of enzyme activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5635051A true JPS5635051A (en) | 1981-04-07 |
Family
ID=14545404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11081779A Pending JPS5635051A (en) | 1979-08-29 | 1979-08-29 | Measuring method of enzyme activity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5635051A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007000877A1 (en) * | 2005-06-28 | 2007-01-04 | Matsushita Electric Industrial Co., Ltd. | Method for determination of lactate dehydrogenase activity in blood serum and apparatus for determination of lactate dehydrogenase activity in blood serum |
-
1979
- 1979-08-29 JP JP11081779A patent/JPS5635051A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007000877A1 (en) * | 2005-06-28 | 2007-01-04 | Matsushita Electric Industrial Co., Ltd. | Method for determination of lactate dehydrogenase activity in blood serum and apparatus for determination of lactate dehydrogenase activity in blood serum |
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