JPS56142457A - Liquid chromatography column device - Google Patents

Liquid chromatography column device

Info

Publication number
JPS56142457A
JPS56142457A JP4714980A JP4714980A JPS56142457A JP S56142457 A JPS56142457 A JP S56142457A JP 4714980 A JP4714980 A JP 4714980A JP 4714980 A JP4714980 A JP 4714980A JP S56142457 A JPS56142457 A JP S56142457A
Authority
JP
Japan
Prior art keywords
sliding plug
stationary phase
separating column
pressure
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4714980A
Other languages
Japanese (ja)
Other versions
JPS6161346B2 (en
Inventor
Junichiro Otsubo
Takefumi Shimizu
Mamoru Takahashi
Kazuyuki Nakagawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Otsuka Pharmaceutical Co Ltd
Original Assignee
Otsuka Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Otsuka Pharmaceutical Co Ltd filed Critical Otsuka Pharmaceutical Co Ltd
Priority to JP4714980A priority Critical patent/JPS56142457A/en
Publication of JPS56142457A publication Critical patent/JPS56142457A/en
Publication of JPS6161346B2 publication Critical patent/JPS6161346B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6021—Adjustable pistons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/522—Physical parameters pressure

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

PURPOSE:To prevent delayed occurrence of gap due to contraction of filler agent, etc., by introducing specimen and moving phase solvent to a stationary phase through a sliding plug provided on the top of a separating column and also applying pressure onto the sliding plug continuously. CONSTITUTION:A sliding plug 7 is provided on the top of a separating column 1, and by introduction of specimen and moving phase solvent to a stationary phase 6 through this sliding plug 7, chromatography is conducted. During operation of this chromatography, a prescribed magnitude of pressure is applied onto the sliding plug 7 through a pressure-applying mechanism. By doing so, a filler agent which constitutes the stationary phase 6 in the separating column 1 is tightly pressed by the sliding plug 7, and therefore, a satisfactorily tight stationary phase can be constituted not by the wet-type filling process which requires complicate operation but by the dry-type filling process which can be operated easily.
JP4714980A 1980-04-09 1980-04-09 Liquid chromatography column device Granted JPS56142457A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4714980A JPS56142457A (en) 1980-04-09 1980-04-09 Liquid chromatography column device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4714980A JPS56142457A (en) 1980-04-09 1980-04-09 Liquid chromatography column device

Publications (2)

Publication Number Publication Date
JPS56142457A true JPS56142457A (en) 1981-11-06
JPS6161346B2 JPS6161346B2 (en) 1986-12-25

Family

ID=12767034

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4714980A Granted JPS56142457A (en) 1980-04-09 1980-04-09 Liquid chromatography column device

Country Status (1)

Country Link
JP (1) JPS56142457A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60179653A (en) * 1983-12-02 1985-09-13 イノヴエルフ Improvement of chromatographic device
EP0330503A2 (en) * 1988-02-25 1989-08-30 Tosoh Corporation Method for forming a gel bed in a column for liquid chromatography and an axially adjustable-type column device used for this method
EP0476995A2 (en) * 1990-09-19 1992-03-25 Amicon Ltd Chromatography column

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60179653A (en) * 1983-12-02 1985-09-13 イノヴエルフ Improvement of chromatographic device
EP0330503A2 (en) * 1988-02-25 1989-08-30 Tosoh Corporation Method for forming a gel bed in a column for liquid chromatography and an axially adjustable-type column device used for this method
JPH01217262A (en) * 1988-02-25 1989-08-30 Tosoh Corp Formation of packing agent packed layer for column for liquid chromatography and a mobile cock type column apparatus used therefor
EP0476995A2 (en) * 1990-09-19 1992-03-25 Amicon Ltd Chromatography column

Also Published As

Publication number Publication date
JPS6161346B2 (en) 1986-12-25

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