JPS56141560A - Reagent composition for measuring ketone body in body fluid - Google Patents

Reagent composition for measuring ketone body in body fluid

Info

Publication number
JPS56141560A
JPS56141560A JP4527080A JP4527080A JPS56141560A JP S56141560 A JPS56141560 A JP S56141560A JP 4527080 A JP4527080 A JP 4527080A JP 4527080 A JP4527080 A JP 4527080A JP S56141560 A JPS56141560 A JP S56141560A
Authority
JP
Japan
Prior art keywords
body fluid
salt
liquid
acetoacetic acid
determined
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4527080A
Other languages
Japanese (ja)
Other versions
JPS6251427B2 (en
Inventor
Yuzo Kosaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Priority to JP4527080A priority Critical patent/JPS56141560A/en
Publication of JPS56141560A publication Critical patent/JPS56141560A/en
Publication of JPS6251427B2 publication Critical patent/JPS6251427B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/64—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving ketones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To detect the ketone body in body fluid easily with good sensitivity by using an aqueous nitroprusside soln. in which yttrium salt is allowed to coexist. CONSTITUTION:An aqueous soln. contg. about 0.005-0.6M/l alkali metallic salt of nitroprusside (preferably, Na, K salt) and about 0.1-2M/l yttrium salt [e.g. YCl3, Y2(SO4)3.8H2O, etc] is used as a coloring reagent, and measurement is made at about 4-7pH. In the case of measuring, for example, ketone body (acetoacetic acid) in body fluid (urine), the above-mentioned coloring reagent is added to the liquid to be examined, and after a fixed time, its absorbancy is determined by using a spectrophotometer, with water as contrast, while the absorbancies of a standard acetoacetic acid and coloring reagent liquid are separately determined, thence the acetoacetic acid concn. in the liquid to be examined is determined by using the formula.
JP4527080A 1980-04-08 1980-04-08 Reagent composition for measuring ketone body in body fluid Granted JPS56141560A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4527080A JPS56141560A (en) 1980-04-08 1980-04-08 Reagent composition for measuring ketone body in body fluid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4527080A JPS56141560A (en) 1980-04-08 1980-04-08 Reagent composition for measuring ketone body in body fluid

Publications (2)

Publication Number Publication Date
JPS56141560A true JPS56141560A (en) 1981-11-05
JPS6251427B2 JPS6251427B2 (en) 1987-10-29

Family

ID=12714613

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4527080A Granted JPS56141560A (en) 1980-04-08 1980-04-08 Reagent composition for measuring ketone body in body fluid

Country Status (1)

Country Link
JP (1) JPS56141560A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4405721A (en) * 1980-03-22 1983-09-20 Behringwerke Aktiengesellschaft Diagnostic agent for the detection of ketone bodies
US4970172A (en) * 1986-12-22 1990-11-13 Abbott Laboratories Method and device for ketone measurements
US5071769A (en) * 1986-12-22 1991-12-10 Abbott Laboratories Method and device for ketone measurement

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4405721A (en) * 1980-03-22 1983-09-20 Behringwerke Aktiengesellschaft Diagnostic agent for the detection of ketone bodies
US4970172A (en) * 1986-12-22 1990-11-13 Abbott Laboratories Method and device for ketone measurements
US5071769A (en) * 1986-12-22 1991-12-10 Abbott Laboratories Method and device for ketone measurement

Also Published As

Publication number Publication date
JPS6251427B2 (en) 1987-10-29

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