JPS56138248A - Enzyme immunity measuring method of artificial woolen gonadotropine - Google Patents

Enzyme immunity measuring method of artificial woolen gonadotropine

Info

Publication number
JPS56138248A
JPS56138248A JP4248480A JP4248480A JPS56138248A JP S56138248 A JPS56138248 A JP S56138248A JP 4248480 A JP4248480 A JP 4248480A JP 4248480 A JP4248480 A JP 4248480A JP S56138248 A JPS56138248 A JP S56138248A
Authority
JP
Japan
Prior art keywords
enzyme
hcg
peptide
instance
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4248480A
Other languages
Japanese (ja)
Other versions
JPS6362698B2 (en
Inventor
Susumu Iwasa
Isamu Yoshida
Koichi Kondo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Takeda Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd
Priority to JP4248480A priority Critical patent/JPS56138248A/en
Priority to DK137181A priority patent/DK159276C/en
Priority to DE8181102360T priority patent/DE3173597D1/en
Priority to EP83111727A priority patent/EP0111216A3/en
Priority to EP81102360A priority patent/EP0037110B1/en
Priority to CA000374165A priority patent/CA1162848A/en
Publication of JPS56138248A publication Critical patent/JPS56138248A/en
Priority to US06/533,619 priority patent/US4517290A/en
Publication of JPS6362698B2 publication Critical patent/JPS6362698B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors

Abstract

PURPOSE:To measure with good singularity and high sensitivity, an artificial woolen gonadotropine (hCG) by using a condensate consisting of specific peptide and labelled enzyme. CONSTITUTION:Peptide shown by the formula I (R shows partial peptide chains of 1-14 pieces containing the 14-th Gly of peptide shown by the formula II), and labelled enzyme (for instance, carbohydrase) are condensed in a water solvent (for instance, phosphoric acid buffer solution of pH6-8) at approx. 4-50 deg.C, and a condensate of peptide and enzyme, which has been obtained is refined by the column chromato-graphy and obtained partially. As for the enzyme immunity measuring method of hCG, the double antigen method is desirable, for instance, a peptide enzyme condensate and a soluble anti hCG antibody are added to an hCG containing test liquid, they are made to react concurrently, and after that, the second antibody which is insoluble against the first antibody is added, activated enzyme in solid phase is measured, and hCG in the test liquid is brought to quantitative analysis.
JP4248480A 1980-03-31 1980-03-31 Enzyme immunity measuring method of artificial woolen gonadotropine Granted JPS56138248A (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP4248480A JPS56138248A (en) 1980-03-31 1980-03-31 Enzyme immunity measuring method of artificial woolen gonadotropine
DK137181A DK159276C (en) 1980-03-31 1981-03-26 PROCEDURE FOR THE ISOLATION OF SPECIFIC ANTIBODIES AND ENZYM IMMUNE DETERMINATION METHOD USING THE ISOLATED ANTIBODY
DE8181102360T DE3173597D1 (en) 1980-03-31 1981-03-28 Specific antibody and its use in an enzyme immuno assay
EP83111727A EP0111216A3 (en) 1980-03-31 1981-03-28 Method for enzyme immunoassay and peptide-enzyme conjugate, its lyophilizate, antibody and kit therefor
EP81102360A EP0037110B1 (en) 1980-03-31 1981-03-28 Specific antibody and its use in an enzyme immuno assay
CA000374165A CA1162848A (en) 1980-03-31 1981-03-30 Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor
US06/533,619 US4517290A (en) 1980-03-31 1983-09-19 Method for enzyme immunoassay and peptide-enzyme conjugate and antibody therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4248480A JPS56138248A (en) 1980-03-31 1980-03-31 Enzyme immunity measuring method of artificial woolen gonadotropine

Publications (2)

Publication Number Publication Date
JPS56138248A true JPS56138248A (en) 1981-10-28
JPS6362698B2 JPS6362698B2 (en) 1988-12-05

Family

ID=12637331

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4248480A Granted JPS56138248A (en) 1980-03-31 1980-03-31 Enzyme immunity measuring method of artificial woolen gonadotropine

Country Status (1)

Country Link
JP (1) JPS56138248A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5781447A (en) * 1980-11-11 1982-05-21 Toyo Jozo Co Ltd Human chorionic gonadotropic hormone c-terminal fragment
CN102756944A (en) * 2011-04-28 2012-10-31 海德堡印刷机械股份公司 Folding machine with conveying apparatus
JP2017508967A (en) * 2014-03-04 2017-03-30 モロジック・リミテッドＭｏｌｏｇｉｃ Ｌｉｍｉｔｅｄ Assay method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5781447A (en) * 1980-11-11 1982-05-21 Toyo Jozo Co Ltd Human chorionic gonadotropic hormone c-terminal fragment
CN102756944A (en) * 2011-04-28 2012-10-31 海德堡印刷机械股份公司 Folding machine with conveying apparatus
JP2017508967A (en) * 2014-03-04 2017-03-30 モロジック・リミテッドＭｏｌｏｇｉｃ Ｌｉｍｉｔｅｄ Assay method

Also Published As

Publication number Publication date
JPS6362698B2 (en) 1988-12-05

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