JPS56122947A - Determination of pyruvic acid by electrode method - Google Patents

Determination of pyruvic acid by electrode method

Info

Publication number
JPS56122947A
JPS56122947A JP2703080A JP2703080A JPS56122947A JP S56122947 A JPS56122947 A JP S56122947A JP 2703080 A JP2703080 A JP 2703080A JP 2703080 A JP2703080 A JP 2703080A JP S56122947 A JPS56122947 A JP S56122947A
Authority
JP
Japan
Prior art keywords
electrode
enzyme
membrane
pop
pyruvic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2703080A
Other languages
Japanese (ja)
Inventor
Koichi Takizawa
Kunio Kaede
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Omron Corp
Original Assignee
Omron Tateisi Electronics Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Omron Tateisi Electronics Co filed Critical Omron Tateisi Electronics Co
Priority to JP2703080A priority Critical patent/JPS56122947A/en
Publication of JPS56122947A publication Critical patent/JPS56122947A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes

Abstract

PURPOSE:To find the concentration of pyruvic acid by utilizing POP reaction, by using the fixation enzyme membrane prepared by immobilized enzyme POP on the porous high molecule membrane which is provided on the surface of electrode as the enzyme electrode. CONSTITUTION:Platinum electrode is used for the working electrode 12 and silver chloride electrode is used for the reference electrode 13. POP bridged on acetylcellulose film by adsorption by 0.025% glutaric aldehyde, is used for the immobilized enzyme membrane 16 and the enzyme electrode 10 is made by attaching the membrane 16 on said electrodes through the oxygen permeating membrane 15 made of teflon. The electrode 10 is contacted with the sample solution and also, applying voltage of electrode is set at -0.5--0.7 constant voltage. Then, decreased quantity of the enzyme accompained by POP reaction, is detected as the variation of current of electrode and the concentration of pyruvic acid is found. Severed kinds of biochemical substance are measured because pyruvic acid is contained in the product product not only by a single reaction of the enzyme but by a conjunction of two or more reactions of the enzyme.
JP2703080A 1980-03-03 1980-03-03 Determination of pyruvic acid by electrode method Pending JPS56122947A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2703080A JPS56122947A (en) 1980-03-03 1980-03-03 Determination of pyruvic acid by electrode method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2703080A JPS56122947A (en) 1980-03-03 1980-03-03 Determination of pyruvic acid by electrode method

Publications (1)

Publication Number Publication Date
JPS56122947A true JPS56122947A (en) 1981-09-26

Family

ID=12209667

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2703080A Pending JPS56122947A (en) 1980-03-03 1980-03-03 Determination of pyruvic acid by electrode method

Country Status (1)

Country Link
JP (1) JPS56122947A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503145A (en) * 1981-02-27 1985-03-05 Fuji Photo Film Co., Ltd. Quantitative analysis film
JPH0295857U (en) * 1988-12-10 1990-07-31
CN112525966A (en) * 2020-11-12 2021-03-19 南京工业大学 Based on H2O2Non-enzyme pyruvic acid sensing detection method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503145A (en) * 1981-02-27 1985-03-05 Fuji Photo Film Co., Ltd. Quantitative analysis film
JPH0295857U (en) * 1988-12-10 1990-07-31
CN112525966A (en) * 2020-11-12 2021-03-19 南京工业大学 Based on H2O2Non-enzyme pyruvic acid sensing detection method

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