JPS5567718A - Optical system of multipurpose photometric microscope - Google Patents

Optical system of multipurpose photometric microscope

Info

Publication number
JPS5567718A
JPS5567718A JP14168078A JP14168078A JPS5567718A JP S5567718 A JPS5567718 A JP S5567718A JP 14168078 A JP14168078 A JP 14168078A JP 14168078 A JP14168078 A JP 14168078A JP S5567718 A JPS5567718 A JP S5567718A
Authority
JP
Japan
Prior art keywords
measurement
fluorescence
optical
optical system
led
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP14168078A
Other languages
Japanese (ja)
Inventor
Satoshi Fujime
Nobuyuki Wakayama
Yukishige Kawasaki
Tadakazu Maeda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP14168078A priority Critical patent/JPS5567718A/en
Publication of JPS5567718A publication Critical patent/JPS5567718A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0096—Microscopes with photometer devices

Landscapes

  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

PURPOSE:To enable various characteristics to be measured with an observation optical system and a photometric optical system by disposing the specified optical elements to a laser generator and subjecting these optical elements to insertion, removal and optical path changeover. CONSTITUTION:A laser beam from a generator 1 is led via filter 2 for intensity adjustment, mirros 3, 4, shutter 5, shaping filter 6 for intensity distribution to a filter 7 which weakens the beam for a certain fixed time and a chopper 8 which cuts off the beam. The above-mentioned 2, 5, 6, 7, 8 are made insertable. Next, an optical path changeover mirror 11 causes only the laser light to be led to a sample 21 by a polarizer 16, radiation aperture 17, 1/4lambda phase plate 18, dichroic mirror 19 and objective lens 20. The fluorescence from the sample is led to an observation optical system 31 and photometirc optical systems 33 thru 40. A polarizer 25, phase plate 26 and condenser lens 27 are disposed to the other system path of the optical path changeover mirror 11. These enable the measurement of fluorescence intensity, the measurement of fluorescence anisotropy, the measurement of the recovery of fluorescent light fading, the measurement of fluorescence correlation, the measurement of absorption dichromaticity and the measurement of double refraction to be accomplished.
JP14168078A 1978-11-16 1978-11-16 Optical system of multipurpose photometric microscope Pending JPS5567718A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14168078A JPS5567718A (en) 1978-11-16 1978-11-16 Optical system of multipurpose photometric microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14168078A JPS5567718A (en) 1978-11-16 1978-11-16 Optical system of multipurpose photometric microscope

Publications (1)

Publication Number Publication Date
JPS5567718A true JPS5567718A (en) 1980-05-22

Family

ID=15297698

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14168078A Pending JPS5567718A (en) 1978-11-16 1978-11-16 Optical system of multipurpose photometric microscope

Country Status (1)

Country Link
JP (1) JPS5567718A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6053916A (en) * 1983-09-05 1985-03-28 Olympus Optical Co Ltd Multi-purpose microscope
EP0164680A2 (en) * 1984-06-05 1985-12-18 Olympus Optical Co., Ltd. Integrated photometric microscope system
EP0510329A2 (en) * 1991-04-26 1992-10-28 Olympus Optical Co., Ltd. Photographing apparatus for microscopes
WO2003091712A1 (en) * 2002-04-24 2003-11-06 Imperial College Innovations Limited Device and method for detecting fluorescence comprising a lightr emitting diode as excitation source

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6053916A (en) * 1983-09-05 1985-03-28 Olympus Optical Co Ltd Multi-purpose microscope
EP0164680A2 (en) * 1984-06-05 1985-12-18 Olympus Optical Co., Ltd. Integrated photometric microscope system
EP0510329A2 (en) * 1991-04-26 1992-10-28 Olympus Optical Co., Ltd. Photographing apparatus for microscopes
WO2003091712A1 (en) * 2002-04-24 2003-11-06 Imperial College Innovations Limited Device and method for detecting fluorescence comprising a lightr emitting diode as excitation source

Similar Documents

Publication Publication Date Title
US6094274A (en) Fluorescence detecting device
CN101216601B (en) Method and device for realizing dark field microscopy and fluorescence microscopy by using cone lens
US5966204A (en) Near-infrared microscope
US6025956A (en) Incident-light fluorescence microscope
US5835262A (en) Multi-wavelength optical microscope
JP2000056228A (en) System for detection by wavelengths to be used for laser scanning microscope, and image recording method
GB1089078A (en) Coaxial illuminator for stereomicroscope
JPS5459951A (en) Transmission lighting device for microscopes
JPS55113014A (en) Coaxial reflected lighting type stereomicroscope
JPS5567718A (en) Optical system of multipurpose photometric microscope
Houghton The transmission of visible light through fog
JPS57178212A (en) Microscope optical system
JPS5632116A (en) Specimen observing device
Liebman et al. [89] Lateral diffusion of visual pigment in rod disk membranes
CN105589186B (en) Micro- Fourier's imaging optical system
JPS57111508A (en) Lighting optical system of microscope for surgical operation
JPS60420A (en) Fluorescence microphotometric device
BLOUT Ultraviolet microscopy and ultraviolet microspectroscopy
JPS57111838A (en) Optical information reader
JPS5466854A (en) Polarized lighting system of binocular stereo microscope
JPH0756092A (en) Vertical illuminating device
SE8303693D0 (en) MICROTOM WITH LIGHTING LIGHT
Pirozzi et al. Optical Components of a Light Microscope
JPS53122471A (en) Fluorescent photometric microscope
RU1805347C (en) Photometer-fluorimeter-nephelometer