JPS55154464A - Production of fixed red corpsucle for measuring lymph cell b cell - Google Patents

Production of fixed red corpsucle for measuring lymph cell b cell

Info

Publication number
JPS55154464A
JPS55154464A JP6151779A JP6151779A JPS55154464A JP S55154464 A JPS55154464 A JP S55154464A JP 6151779 A JP6151779 A JP 6151779A JP 6151779 A JP6151779 A JP 6151779A JP S55154464 A JPS55154464 A JP S55154464A
Authority
JP
Japan
Prior art keywords
red
corpsucles
cells
complement
sheep
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6151779A
Other languages
Japanese (ja)
Other versions
JPS6262288B2 (en
Inventor
Shosaku Motoda
Michiaki Origasa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyocera Chemical Corp
Original Assignee
Toshiba Chemical Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toshiba Chemical Corp filed Critical Toshiba Chemical Corp
Priority to JP6151779A priority Critical patent/JPS55154464A/en
Publication of JPS55154464A publication Critical patent/JPS55154464A/en
Publication of JPS6262288B2 publication Critical patent/JPS6262288B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE: To obtain the fixed red corpuscles which are superior in shelf life, and are capable of singularily forming rosette only in the B cells by treating the red corpsucles of a sheep or the like by aldehydes and causing immune globulins M and complement to react and couple with these red corpsucles.
CONSTITUTION: In the production of the red corpsucles fixing the B cells in lymph cells in order to clarify the immunity phenomena of cancer, viral diseases, etc., the fresh blood sampled from the carotid artery of a sheep is centrifugally separated, after which the supernatant is discarded and cleaned and the red corpsucles are suspended in a phosphoric acid buffer solution of pH7.0W 7.4, to which glutaraldehyde is added and the solution is treated at 28°C, whereby the cell membranes ar fixed. The immune globlins M obtained by injecting the sheep red corpsucles into a rabbit are mixed in the fixed red corpsucles. Next, the complement in the mouse serum is coupled with the foregoing globulins M, whereby the sensitized red corpsucles coupled with the complement C3 by way of the immune globulines M are obtained. These sensitized red corpsucles permit storage of about one month and do not cause any degradation in the rosette forming capacity as against the B cells. It is also possible to store these by adding stabilizers such as lactose thereto, followed by freezing and drying.
COPYRIGHT: (C)1980,JPO&Japio
JP6151779A 1979-05-21 1979-05-21 Production of fixed red corpsucle for measuring lymph cell b cell Granted JPS55154464A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6151779A JPS55154464A (en) 1979-05-21 1979-05-21 Production of fixed red corpsucle for measuring lymph cell b cell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6151779A JPS55154464A (en) 1979-05-21 1979-05-21 Production of fixed red corpsucle for measuring lymph cell b cell

Publications (2)

Publication Number Publication Date
JPS55154464A true JPS55154464A (en) 1980-12-02
JPS6262288B2 JPS6262288B2 (en) 1987-12-25

Family

ID=13173353

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6151779A Granted JPS55154464A (en) 1979-05-21 1979-05-21 Production of fixed red corpsucle for measuring lymph cell b cell

Country Status (1)

Country Link
JP (1) JPS55154464A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109060972A (en) * 2018-05-16 2018-12-21 苏州药明泽康生物科技有限公司 Application of the rabbit blood in preparation human disease's external diagnosis reagent case

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109060972A (en) * 2018-05-16 2018-12-21 苏州药明泽康生物科技有限公司 Application of the rabbit blood in preparation human disease's external diagnosis reagent case
CN109060972B (en) * 2018-05-16 2021-09-24 苏州药明泽康生物科技有限公司 Application of rabbit blood in preparing human disease in-vitro diagnosis kit

Also Published As

Publication number Publication date
JPS6262288B2 (en) 1987-12-25

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