JPS55154464A - Production of fixed red corpsucle for measuring lymph cell b cell - Google Patents
Production of fixed red corpsucle for measuring lymph cell b cellInfo
- Publication number
- JPS55154464A JPS55154464A JP6151779A JP6151779A JPS55154464A JP S55154464 A JPS55154464 A JP S55154464A JP 6151779 A JP6151779 A JP 6151779A JP 6151779 A JP6151779 A JP 6151779A JP S55154464 A JPS55154464 A JP S55154464A
- Authority
- JP
- Japan
- Prior art keywords
- red
- corpsucles
- cells
- complement
- sheep
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To obtain the fixed red corpuscles which are superior in shelf life, and are capable of singularily forming rosette only in the B cells by treating the red corpsucles of a sheep or the like by aldehydes and causing immune globulins M and complement to react and couple with these red corpsucles.
CONSTITUTION: In the production of the red corpsucles fixing the B cells in lymph cells in order to clarify the immunity phenomena of cancer, viral diseases, etc., the fresh blood sampled from the carotid artery of a sheep is centrifugally separated, after which the supernatant is discarded and cleaned and the red corpsucles are suspended in a phosphoric acid buffer solution of pH7.0W 7.4, to which glutaraldehyde is added and the solution is treated at 28°C, whereby the cell membranes ar fixed. The immune globlins M obtained by injecting the sheep red corpsucles into a rabbit are mixed in the fixed red corpsucles. Next, the complement in the mouse serum is coupled with the foregoing globulins M, whereby the sensitized red corpsucles coupled with the complement C3 by way of the immune globulines M are obtained. These sensitized red corpsucles permit storage of about one month and do not cause any degradation in the rosette forming capacity as against the B cells. It is also possible to store these by adding stabilizers such as lactose thereto, followed by freezing and drying.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6151779A JPS55154464A (en) | 1979-05-21 | 1979-05-21 | Production of fixed red corpsucle for measuring lymph cell b cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP6151779A JPS55154464A (en) | 1979-05-21 | 1979-05-21 | Production of fixed red corpsucle for measuring lymph cell b cell |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS55154464A true JPS55154464A (en) | 1980-12-02 |
JPS6262288B2 JPS6262288B2 (en) | 1987-12-25 |
Family
ID=13173353
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP6151779A Granted JPS55154464A (en) | 1979-05-21 | 1979-05-21 | Production of fixed red corpsucle for measuring lymph cell b cell |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS55154464A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109060972A (en) * | 2018-05-16 | 2018-12-21 | 苏州药明泽康生物科技有限公司 | Application of the rabbit blood in preparation human disease's external diagnosis reagent case |
-
1979
- 1979-05-21 JP JP6151779A patent/JPS55154464A/en active Granted
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109060972A (en) * | 2018-05-16 | 2018-12-21 | 苏州药明泽康生物科技有限公司 | Application of the rabbit blood in preparation human disease's external diagnosis reagent case |
CN109060972B (en) * | 2018-05-16 | 2021-09-24 | 苏州药明泽康生物科技有限公司 | Application of rabbit blood in preparing human disease in-vitro diagnosis kit |
Also Published As
Publication number | Publication date |
---|---|
JPS6262288B2 (en) | 1987-12-25 |
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