JPS55147347A - Process for analyzing bile acid - Google Patents
Process for analyzing bile acidInfo
- Publication number
- JPS55147347A JPS55147347A JP5501679A JP5501679A JPS55147347A JP S55147347 A JPS55147347 A JP S55147347A JP 5501679 A JP5501679 A JP 5501679A JP 5501679 A JP5501679 A JP 5501679A JP S55147347 A JPS55147347 A JP S55147347A
- Authority
- JP
- Japan
- Prior art keywords
- bile acid
- compounds
- fluorescences
- liquid chromatography
- esterified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To analyze and quantitatively measure bile acid correctly in a short time by converting conjugated bile acid and free bile acid into fluorescent substances, thereafter separating them by liquid chromatography, and measurring the fluorescences thereof.
CONSTITUTION: Bile acids including both conjugated and free types, derived from a sample to be analyzed are esterified using as an esterification reagent, 4-bromomethyl-7-methoxy coumarin in the presence of a crown ether, and converted into bile acid esterfied compounds having fluorescences. Thereafter, the bile acid esterifed compounds are separated respectively by liquid chromatography. The thus separated bile acid esterified compounds are respectively subjected to fluometric analysis.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5501679A JPS55147347A (en) | 1979-05-04 | 1979-05-04 | Process for analyzing bile acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5501679A JPS55147347A (en) | 1979-05-04 | 1979-05-04 | Process for analyzing bile acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS55147347A true JPS55147347A (en) | 1980-11-17 |
Family
ID=12986854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5501679A Pending JPS55147347A (en) | 1979-05-04 | 1979-05-04 | Process for analyzing bile acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55147347A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115420825A (en) * | 2022-08-31 | 2022-12-02 | 北京大学第三医院（北京大学第三临床医学院） | Bile acid detection method and bile acid derivative |
-
1979
- 1979-05-04 JP JP5501679A patent/JPS55147347A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115420825A (en) * | 2022-08-31 | 2022-12-02 | 北京大学第三医院（北京大学第三临床医学院） | Bile acid detection method and bile acid derivative |
| CN115420825B (en) * | 2022-08-31 | 2023-12-22 | 北京大学第三医院（北京大学第三临床医学院） | Method for detecting bile acid and bile acid derivative |
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