JPS55122153A - Removing protein from sample liquid for analysis - Google Patents
Removing protein from sample liquid for analysisInfo
- Publication number
- JPS55122153A JPS55122153A JP2879479A JP2879479A JPS55122153A JP S55122153 A JPS55122153 A JP S55122153A JP 2879479 A JP2879479 A JP 2879479A JP 2879479 A JP2879479 A JP 2879479A JP S55122153 A JPS55122153 A JP S55122153A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- sample liquid
- analysis
- liquid
- microwaves
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To simply and speedily remove protein from a sample liquid by irradiating a protein-containing sample liquid with microwaves at a concentration of hydrogen ion close to an isoelectric point of the protein to heat and solidify the protein in a short period of time.
CONSTITUTION: A sample liquid for analysis is diluted with demineralized water. Hydrochloric acid or acetic acid is added to the diluted liquid to adjust the pH of the resulting mixture to 4W6, preferably 5W5.5 which is in the vicinity of an isoelectric point of protein. The mixture thus obtained is then irradiated with microwaves of an outut of about 100W1,500 watt, preferably 300W600watt, and a frequency of about 500W5,000MHz, preferably 1500W3000MHz, to heat and solidify the protein contained in the sample liquid. The sample liquid is then quenched to room temperature and subjected to centrifugal separation to remove the protein. The supernatant portion of the liquid thus obtained is subjected to analysis.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2879479A JPS55122153A (en) | 1979-03-14 | 1979-03-14 | Removing protein from sample liquid for analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2879479A JPS55122153A (en) | 1979-03-14 | 1979-03-14 | Removing protein from sample liquid for analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS55122153A true JPS55122153A (en) | 1980-09-19 |
Family
ID=12258321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2879479A Pending JPS55122153A (en) | 1979-03-14 | 1979-03-14 | Removing protein from sample liquid for analysis |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55122153A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04127059A (en) * | 1990-09-19 | 1992-04-28 | Michirou Shibazaki | Method and apparatus for separating serum |
| JP2016516680A (en) * | 2013-03-15 | 2016-06-09 | アイエスピー インヴェストメンツ インコーポレイテッドＩｓｐ Ｉｎｖｅｓｔｍｅｎｔｓ Ｉｎｃ． | Biologically active composition derived from fruit and method for producing the same |
| WO2023000769A1 (en) * | 2021-07-19 | 2023-01-26 | 山东恒鲁生物科技有限公司 | Qualitative and quantitative detection method for human milk oligosaccharides in dairy product |
-
1979
- 1979-03-14 JP JP2879479A patent/JPS55122153A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04127059A (en) * | 1990-09-19 | 1992-04-28 | Michirou Shibazaki | Method and apparatus for separating serum |
| JP2016516680A (en) * | 2013-03-15 | 2016-06-09 | アイエスピー インヴェストメンツ インコーポレイテッドＩｓｐ Ｉｎｖｅｓｔｍｅｎｔｓ Ｉｎｃ． | Biologically active composition derived from fruit and method for producing the same |
| WO2023000769A1 (en) * | 2021-07-19 | 2023-01-26 | 山东恒鲁生物科技有限公司 | Qualitative and quantitative detection method for human milk oligosaccharides in dairy product |
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