JPS5428896A - Preparation of plasmid - Google Patents

Preparation of plasmid

Info

Publication number
JPS5428896A
JPS5428896A JP9476777A JP9476777A JPS5428896A JP S5428896 A JPS5428896 A JP S5428896A JP 9476777 A JP9476777 A JP 9476777A JP 9476777 A JP9476777 A JP 9476777A JP S5428896 A JPS5428896 A JP S5428896A
Authority
JP
Japan
Prior art keywords
plasmid
preparation
density gradient
microorganisms belonging
separate pure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9476777A
Other languages
Japanese (ja)
Other versions
JPS6137916B2 (en
Inventor
Kenji Sakaguchi
Fumio Hishinuma
Teruo Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP9476777A priority Critical patent/JPS5428896A/en
Publication of JPS5428896A publication Critical patent/JPS5428896A/en
Publication of JPS6137916B2 publication Critical patent/JPS6137916B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE:To separate pure plasmid from microorganisms belonging to Thermus genus by density gradient centrifuging.
JP9476777A 1977-08-08 1977-08-08 Preparation of plasmid Granted JPS5428896A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9476777A JPS5428896A (en) 1977-08-08 1977-08-08 Preparation of plasmid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9476777A JPS5428896A (en) 1977-08-08 1977-08-08 Preparation of plasmid

Publications (2)

Publication Number Publication Date
JPS5428896A true JPS5428896A (en) 1979-03-03
JPS6137916B2 JPS6137916B2 (en) 1986-08-26

Family

ID=14119240

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9476777A Granted JPS5428896A (en) 1977-08-08 1977-08-08 Preparation of plasmid

Country Status (1)

Country Link
JP (1) JPS5428896A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434631A (en) * 2016-09-28 2017-02-22 深圳精准医疗科技有限公司 Method for extracting large number of plasmids and applications of method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434631A (en) * 2016-09-28 2017-02-22 深圳精准医疗科技有限公司 Method for extracting large number of plasmids and applications of method
CN106434631B (en) * 2016-09-28 2019-07-26 深圳精准医疗科技有限公司 A kind of method and its application of large quantity extracting plasmid

Also Published As

Publication number Publication date
JPS6137916B2 (en) 1986-08-26

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