JPS54145191A - Separating method of mixture - Google Patents

Separating method of mixture

Info

Publication number
JPS54145191A
JPS54145191A JP5247978A JP5247978A JPS54145191A JP S54145191 A JPS54145191 A JP S54145191A JP 5247978 A JP5247978 A JP 5247978A JP 5247978 A JP5247978 A JP 5247978A JP S54145191 A JPS54145191 A JP S54145191A
Authority
JP
Japan
Prior art keywords
column
chromato
tube
petroleum ether
mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP5247978A
Other languages
Japanese (ja)
Other versions
JPS5644377B2 (en
Inventor
Keiji Iriyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP5247978A priority Critical patent/JPS54145191A/en
Publication of JPS54145191A publication Critical patent/JPS54145191A/en
Publication of JPS5644377B2 publication Critical patent/JPS5644377B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8411—Intermediate storage of effluent, including condensation on surface
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/80—Fraction collectors

Abstract

PURPOSE: To enable the mixture to be separated to each component readily and rapidly by stopping the development by a developing solvent when the mixture is separated into each component in a column made of the material that can be cut and cutting the column of the required components for each chromato tube.
CONSTITUTION: A column chromato tube 1 is made by using easy-to-mold polypropylene and glass wool is laid on the bottom thereof, thence powder sugar is filled therein. This is connected to a suction bottle 4' and petroleum ether is flowed from above while being sucked. On the other hand, spinach is acetone-extracted and the extract liquid is evaporated. The resultant black greenish solid is dispersed in petroleum ether to prepare a dispersion. Pouring this dispersion into the column and subsequent development with petroleum ether yield the chromatogram of separation state. Here, the development is suspended and the column is cut for each chromato tube in each dye part region to separately take out the powder sugar adsorbed with each dye. These are respectively extracted with ether, whereby the respective components are obtainable as pure components.
COPYRIGHT: (C)1979,JPO&Japio
JP5247978A 1978-05-01 1978-05-01 Separating method of mixture Granted JPS54145191A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP5247978A JPS54145191A (en) 1978-05-01 1978-05-01 Separating method of mixture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5247978A JPS54145191A (en) 1978-05-01 1978-05-01 Separating method of mixture

Publications (2)

Publication Number Publication Date
JPS54145191A true JPS54145191A (en) 1979-11-13
JPS5644377B2 JPS5644377B2 (en) 1981-10-19

Family

ID=12915849

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5247978A Granted JPS54145191A (en) 1978-05-01 1978-05-01 Separating method of mixture

Country Status (1)

Country Link
JP (1) JPS54145191A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60186734A (en) * 1984-03-06 1985-09-24 Mitsubishi Heavy Ind Ltd Method for measuring trace water in crude oil or petroleum product
WO2012005353A1 (en) * 2010-07-08 2012-01-12 ダイセル化学工業株式会社 Separation/detection column and kit thereof
JP2012202765A (en) * 2011-03-24 2012-10-22 Kurita Water Ind Ltd Column assembly, fluid processing column, and its characteristic measuring method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60186734A (en) * 1984-03-06 1985-09-24 Mitsubishi Heavy Ind Ltd Method for measuring trace water in crude oil or petroleum product
WO2012005353A1 (en) * 2010-07-08 2012-01-12 ダイセル化学工業株式会社 Separation/detection column and kit thereof
US9057700B2 (en) 2010-07-08 2015-06-16 Daicel Corporation Separation/detection column and kit thereof
JP5771206B2 (en) * 2010-07-08 2015-08-26 株式会社ダイセル Separation detection column and kit
JP2012202765A (en) * 2011-03-24 2012-10-22 Kurita Water Ind Ltd Column assembly, fluid processing column, and its characteristic measuring method

Also Published As

Publication number Publication date
JPS5644377B2 (en) 1981-10-19

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