JPS54140790A - Immobilized enzyme - Google Patents
Immobilized enzymeInfo
- Publication number
- JPS54140790A JPS54140790A JP4848978A JP4848978A JPS54140790A JP S54140790 A JPS54140790 A JP S54140790A JP 4848978 A JP4848978 A JP 4848978A JP 4848978 A JP4848978 A JP 4848978A JP S54140790 A JPS54140790 A JP S54140790A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- film
- immobilized enzyme
- molecular weight
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE: To prepare an immobilized enzyme having high actvity, long life, and specific structure, easily, by a specific process.
CONSTITUTION: An enzyme (e.g. glucose oxidase, L-amino acid oxidase, lactase, etc.) is homogeneously dispersed in an aqueous medium together with (A) polyvinyl pyrrolidone, or (B) polyvinyl alcohol, and (C) a polyfunctional compound having two or more photo-polymerizable functional groups. The dispersion is applied to a substrate to a thin film, the solvent is evaporated, and the film is polymerized by irradiating with light to afford an insoluble film containing immobilized enzyme on the substrate. The film has a thickness of ≤30μ, and is composed of a three- dimensional matrix of (C) polymer having a molecular weight of ≤450 dispersed with a water-soluble polymer of (A) having a molecular weight of ≥100,000 or (B) having a polymerization degree of ≥1,000 and a saponification rate of ≥75%, and the enzyme.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4848978A JPS54140790A (en) | 1978-04-24 | 1978-04-24 | Immobilized enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4848978A JPS54140790A (en) | 1978-04-24 | 1978-04-24 | Immobilized enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS54140790A true JPS54140790A (en) | 1979-11-01 |
Family
ID=12804795
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4848978A Pending JPS54140790A (en) | 1978-04-24 | 1978-04-24 | Immobilized enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54140790A (en) |
-
1978
- 1978-04-24 JP JP4848978A patent/JPS54140790A/en active Pending
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