JPS54107591A - Preparation of thioesterase - Google Patents

Preparation of thioesterase

Info

Publication number
JPS54107591A
JPS54107591A JP1479478A JP1479478A JPS54107591A JP S54107591 A JPS54107591 A JP S54107591A JP 1479478 A JP1479478 A JP 1479478A JP 1479478 A JP1479478 A JP 1479478A JP S54107591 A JPS54107591 A JP S54107591A
Authority
JP
Japan
Prior art keywords
thioesterase
cells
separating
pseudomonas aeruginosa
centrifugal separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1479478A
Other languages
Japanese (ja)
Other versions
JPS6317435B2 (en
Inventor
Makoto Kageyama
Izumi Okawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP1479478A priority Critical patent/JPS54107591A/en
Publication of JPS54107591A publication Critical patent/JPS54107591A/en
Publication of JPS6317435B2 publication Critical patent/JPS6317435B2/ja
Granted legal-status Critical Current

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  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE: To prepare thioesterase useful as a reagent for the determination of acyl- coenzyme A, easily, by disintegrating cells of Pseudomonas aeruginosa and separating the cell membrane by centrifugal separation.
CONSTITUTION: Cells of Pseudomonas aeruginosa are disintegrated by French press, ultrasonic treatment, lysozyme treatment, etc., and centrifuged to obtain cell membrane fraction containing thioesterase. The centrifugal separation is carried out, e.g. by roughly separating the undisintegrated cells at 5000g for 10 min. and then separating the membrane at 30000g for 30 min. using an ultracentrifuging apparatus.
COPYRIGHT: (C)1979,JPO&Japio
JP1479478A 1978-02-10 1978-02-10 Preparation of thioesterase Granted JPS54107591A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1479478A JPS54107591A (en) 1978-02-10 1978-02-10 Preparation of thioesterase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1479478A JPS54107591A (en) 1978-02-10 1978-02-10 Preparation of thioesterase

Publications (2)

Publication Number Publication Date
JPS54107591A true JPS54107591A (en) 1979-08-23
JPS6317435B2 JPS6317435B2 (en) 1988-04-13

Family

ID=11870951

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1479478A Granted JPS54107591A (en) 1978-02-10 1978-02-10 Preparation of thioesterase

Country Status (1)

Country Link
JP (1) JPS54107591A (en)

Also Published As

Publication number Publication date
JPS6317435B2 (en) 1988-04-13

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