JPS54107591A - Preparation of thioesterase - Google Patents
Preparation of thioesteraseInfo
- Publication number
- JPS54107591A JPS54107591A JP1479478A JP1479478A JPS54107591A JP S54107591 A JPS54107591 A JP S54107591A JP 1479478 A JP1479478 A JP 1479478A JP 1479478 A JP1479478 A JP 1479478A JP S54107591 A JPS54107591 A JP S54107591A
- Authority
- JP
- Japan
- Prior art keywords
- thioesterase
- cells
- separating
- pseudomonas aeruginosa
- centrifugal separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare thioesterase useful as a reagent for the determination of acyl- coenzyme A, easily, by disintegrating cells of Pseudomonas aeruginosa and separating the cell membrane by centrifugal separation.
CONSTITUTION: Cells of Pseudomonas aeruginosa are disintegrated by French press, ultrasonic treatment, lysozyme treatment, etc., and centrifuged to obtain cell membrane fraction containing thioesterase. The centrifugal separation is carried out, e.g. by roughly separating the undisintegrated cells at 5000g for 10 min. and then separating the membrane at 30000g for 30 min. using an ultracentrifuging apparatus.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1479478A JPS54107591A (en) | 1978-02-10 | 1978-02-10 | Preparation of thioesterase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1479478A JPS54107591A (en) | 1978-02-10 | 1978-02-10 | Preparation of thioesterase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS54107591A true JPS54107591A (en) | 1979-08-23 |
JPS6317435B2 JPS6317435B2 (en) | 1988-04-13 |
Family
ID=11870951
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1479478A Granted JPS54107591A (en) | 1978-02-10 | 1978-02-10 | Preparation of thioesterase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS54107591A (en) |
-
1978
- 1978-02-10 JP JP1479478A patent/JPS54107591A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS6317435B2 (en) | 1988-04-13 |
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