JPS54105597A - Measuring method of oxide type substrate concentration and enzyme electrode - Google Patents
Measuring method of oxide type substrate concentration and enzyme electrodeInfo
- Publication number
- JPS54105597A JPS54105597A JP11825377A JP11825377A JPS54105597A JP S54105597 A JPS54105597 A JP S54105597A JP 11825377 A JP11825377 A JP 11825377A JP 11825377 A JP11825377 A JP 11825377A JP S54105597 A JPS54105597 A JP S54105597A
- Authority
- JP
- Japan
- Prior art keywords
- oxide type
- buffer
- type substrate
- electrode
- instance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To realize measurement of concentration of oxide type substrate of oxidoreductase, by using oxide type substrate as the substrate, and selecting a substance having a baser potential than the oxidation-reduction potential of coenzyme as the redox compound.
CONSTITUTION: An enzyme electrode 1 having a silver plate 3 dipped as the current collector in a buffer 2 such as phosphoric acid is communicated with a counter electrode 4 having a platinum current collector 6 dipped in a buffer 5 by way of a separator 9. The electrode 1 is further connected to a saturated calomel reference electrode 7 through a salt bridge 8. First, a redox compound, for instance, methyl viologen (oxide type) is dissolved in the buffer 2 to be turned into a reduced type at a potential of -0.5V with respect to the electrode 7. Next, a coenzyme, for instance, HADH, and an enzyme, for instance, alcohol dehydrogenase, are dissolved in the buffer 2. When the cathode current reaches the stationary value at the silver plate 3, an oxide type substrate, for instance, acetaldehyde, is added to the buffer 2, so that the concentration of the oxide type substrate may be determined from the increment of the cathod current.
COPYRIGHT: (C)1979,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP52118253A JPS5853747B2 (en) | 1977-09-30 | 1977-09-30 | Substrate concentration measurement method and enzyme electrode system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP52118253A JPS5853747B2 (en) | 1977-09-30 | 1977-09-30 | Substrate concentration measurement method and enzyme electrode system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54105597A true JPS54105597A (en) | 1979-08-18 |
| JPS5853747B2 JPS5853747B2 (en) | 1983-12-01 |
Family
ID=14732026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP52118253A Expired JPS5853747B2 (en) | 1977-09-30 | 1977-09-30 | Substrate concentration measurement method and enzyme electrode system |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5853747B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5692445A (en) * | 1979-12-25 | 1981-07-27 | Omron Tateisi Electronics Co | Quantitative analytical method of got |
| JPS5692446A (en) * | 1979-12-25 | 1981-07-27 | Omron Tateisi Electronics Co | Quantitative analytical method of gpt |
| JP2012237743A (en) * | 2011-04-26 | 2012-12-06 | Arkray Inc | Analysis tool |
-
1977
- 1977-09-30 JP JP52118253A patent/JPS5853747B2/en not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5692445A (en) * | 1979-12-25 | 1981-07-27 | Omron Tateisi Electronics Co | Quantitative analytical method of got |
| JPS5692446A (en) * | 1979-12-25 | 1981-07-27 | Omron Tateisi Electronics Co | Quantitative analytical method of gpt |
| JP2012237743A (en) * | 2011-04-26 | 2012-12-06 | Arkray Inc | Analysis tool |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5853747B2 (en) | 1983-12-01 |
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