JPH11346648A - Stabilizer for fermented milk drink and production of fermented milk drink using the same - Google Patents
Stabilizer for fermented milk drink and production of fermented milk drink using the sameInfo
- Publication number
- JPH11346648A JPH11346648A JP10154388A JP15438898A JPH11346648A JP H11346648 A JPH11346648 A JP H11346648A JP 10154388 A JP10154388 A JP 10154388A JP 15438898 A JP15438898 A JP 15438898A JP H11346648 A JPH11346648 A JP H11346648A
- Authority
- JP
- Japan
- Prior art keywords
- fermented milk
- stabilizer
- milk
- milk drink
- cmc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000015140 cultured milk Nutrition 0.000 title claims abstract description 79
- 239000003381 stabilizer Substances 0.000 title claims abstract description 37
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 36
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims abstract description 36
- 239000007864 aqueous solution Substances 0.000 claims abstract description 20
- 239000002253 acid Substances 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 238000006266 etherification reaction Methods 0.000 claims abstract description 8
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 5
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims abstract description 5
- 150000003839 salts Chemical class 0.000 claims abstract description 4
- 235000013361 beverage Nutrition 0.000 claims description 30
- 102000014171 Milk Proteins Human genes 0.000 claims description 16
- 108010011756 Milk Proteins Proteins 0.000 claims description 16
- 235000021239 milk protein Nutrition 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000007787 solid Substances 0.000 abstract description 16
- 238000000926 separation method Methods 0.000 abstract description 13
- 239000000243 solution Substances 0.000 abstract description 6
- 238000001556 precipitation Methods 0.000 abstract description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 235000013336 milk Nutrition 0.000 description 16
- 239000008267 milk Substances 0.000 description 16
- 210000004080 milk Anatomy 0.000 description 16
- 239000000843 powder Substances 0.000 description 16
- 239000000203 mixture Substances 0.000 description 14
- 235000020183 skimmed milk Nutrition 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 12
- 239000005018 casein Substances 0.000 description 10
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 10
- 235000021240 caseins Nutrition 0.000 description 10
- 239000004310 lactic acid Substances 0.000 description 10
- 235000014655 lactic acid Nutrition 0.000 description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 238000005345 coagulation Methods 0.000 description 9
- 230000015271 coagulation Effects 0.000 description 9
- 239000002245 particle Substances 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000002244 precipitate Substances 0.000 description 6
- 229920000742 Cotton Polymers 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 4
- 229910052782 aluminium Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000011888 foil Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 238000004220 aggregation Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 235000013861 fat-free Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000001727 glucose Nutrition 0.000 description 3
- 235000015205 orange juice Nutrition 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- 230000005653 Brownian motion process Effects 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 150000008064 anhydrides Chemical class 0.000 description 2
- 238000005537 brownian motion Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- HDSBZMRLPLPFLQ-UHFFFAOYSA-N Propylene glycol alginate Chemical compound OC1C(O)C(OC)OC(C(O)=O)C1OC1C(O)C(O)C(C)C(C(=O)OCC(C)O)O1 HDSBZMRLPLPFLQ-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000002535 acidifier Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229920006184 cellulose methylcellulose Polymers 0.000 description 1
- 239000011153 ceramic matrix composite Substances 0.000 description 1
- 238000012710 chemistry, manufacturing and control Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 1
- 229910001950 potassium oxide Inorganic materials 0.000 description 1
- 239000000770 propane-1,2-diol alginate Substances 0.000 description 1
- 235000010409 propane-1,2-diol alginate Nutrition 0.000 description 1
- 230000004845 protein aggregation Effects 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 150000004685 tetrahydrates Chemical class 0.000 description 1
Landscapes
- Dairy Products (AREA)
- Jellies, Jams, And Syrups (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は醗酵乳飲料用安定剤
およびそれを用いた醗酵乳飲料の製法に関する。さらに
詳しくは、沈殿や分離が生じない安定性の高い醗酵乳飲
料の製造に用いる醗酵乳飲料用安定剤およびそれを用い
た醗酵乳飲料の製法に関する。The present invention relates to a stabilizer for fermented milk drinks and a method for producing a fermented milk drink using the same. More particularly, the present invention relates to a stabilizer for a fermented milk beverage used for producing a highly stable fermented milk beverage which does not cause precipitation and separation, and a method for producing a fermented milk beverage using the same.
【0002】[0002]
【従来の技術および解決しようとする課題】醗酵乳飲料
には、カゼインを主体とする乳蛋白が含まれているが、
これは酸性域において凝集分離して沈殿する。醗酵乳飲
料中で前記凝集分離がおこれば、醗酵乳飲料の外観を損
い、商品価値が低下する。醗酵乳飲料の品質トラブルの
大半は凝集沈殿が原因である。2. Description of the Related Art Fermented milk drinks contain milk proteins mainly containing casein.
This precipitates by coagulation and separation in an acidic region. If the coagulation separation occurs in the fermented milk beverage, the appearance of the fermented milk beverage is impaired, and the commercial value is reduced. Most of the quality problems of fermented milk drinks are caused by flocculation.
【0003】カゼインは単一タンパク質ではなく、超遠
心分離法によって測定した分子量の概数が75000〜
375000の分子の混合物であり、これ以上の集合が
おきない限り乳蛋白は沈殿しない。[0003] Casein is not a single protein, but has an approximate molecular weight of 75,000-
It is a mixture of 375,000 molecules and milk protein will not precipitate unless further aggregation occurs.
【0004】しかし、乳酸菌などの繁殖により乳酸が生
産され、乳蛋白のpHがカゼインの等電点である4.6
に近づけば、電気的斥力が小さくなるため、ブラウン運
動による衝突の際に、分子引力も加わって集合し、大粒
子となったものから沈殿する。However, lactic acid is produced by propagation of lactic acid bacteria and the like, and the pH of milk protein is 4.6, which is the isoelectric point of casein.
Since the electric repulsion becomes smaller as the distance approaches, molecular collisions are also applied during collision by Brownian motion, so that the particles aggregate and precipitate from large particles.
【0005】また、カゼイン粒子がかなりの大きさをも
ち、ブラウン運動のみでは水中で重力に打ち勝つことが
できず、荷電粒子間の反発力の助けをえてようやく支え
られているものが混在するばあいには、等電点に近づい
てその電荷を失えば、前記大きなカゼイン粒子は集合し
なくても沈殿するものが生じる。[0005] In addition, when the casein particles have a considerable size, they cannot overcome gravity in water only by Brownian motion, and some are supported only with the help of repulsion between charged particles. In this case, if the charge is lost due to approaching the isoelectric point, the large casein particles may precipitate even if they do not aggregate.
【0006】実際には、乳酸菌が繁殖してある程度乳酸
が生成すれば、含有されているカゼインが集合し、沈殿
凝固して、いわゆる醗酵乳を形成する。この醗酵乳を砕
いて撹拌すればカゼインの懸濁液がえられるが、これを
静置すれば、再び集合して沈殿する。In practice, if lactic acid bacteria multiply and produce lactic acid to a certain extent, the contained casein aggregates and precipitates and solidifies to form so-called fermented milk. If this fermented milk is crushed and agitated, a casein suspension is obtained, but if it is allowed to stand, it will aggregate and precipitate again.
【0007】前記凝集分離を防止する方法としては、ペ
クチン、アルギン酸ソーダ、カルボキシメチルセルロー
ス塩(CMC)などの安定剤を添加する方法があり、実
製造では、ほとんどのメーカーで採用されている。As a method for preventing the coagulation separation, there is a method of adding a stabilizer such as pectin, sodium alginate, carboxymethylcellulose salt (CMC) and the like, which is adopted by most manufacturers in actual production.
【0008】しかし、醗酵乳飲料の凝集分離防止は、従
来の安定剤を添加する方法のみでは充分解決できるに至
っていない。その理由は、安定剤としての機能が安定剤
の種類によって、また、同じ安定剤であっても安定性に
対する効果がロットナンバーごとに大きく異なるからで
ある。たとえば一般の安定剤は、耐酸性がないため、p
Hが低いとカゼイン粒子表面に形成された安定剤の被膜
が破れ、カゼイン粒子は再び集合凝固する。また、たと
えば耐酸性に優れた安定剤であるCMCを用いると、液
の粘度が増加し、カゼイン粒子の表面に親水基をもつC
MCの被膜が生成し、安定したサスペンション(Sus
pension)となり、水で10倍に薄めて静置して
も沈殿しないようになるが、ロットナンバーが異なるこ
とによる性能のバラツキが大きい。However, the prevention of coagulation and separation of fermented milk beverages has not been sufficiently solved only by the conventional method of adding a stabilizer. The reason is that the function as a stabilizer differs greatly depending on the kind of the stabilizer, and even if the same stabilizer is used, the effect on the stability greatly differs depending on the lot number. For example, general stabilizers have no acid resistance, and
When H is low, the stabilizer film formed on the casein particle surface is broken, and the casein particles collectively solidify again. Further, for example, when CMC which is a stabilizer having excellent acid resistance is used, the viscosity of the solution increases, and CMC having a hydrophilic group on the surface of casein particles is used.
MC film is formed and stable suspension (Sus
Penetration), and even if it is diluted 10 times with water and left to stand, sedimentation does not occur, but there is a large variation in performance due to different lot numbers.
【0009】よって、耐酸性に優れたCMCを安定剤と
して使用することは大変有用なことになる。Therefore, it is very useful to use CMC having excellent acid resistance as a stabilizer.
【0010】[0010]
【課題を解決するための手段】本発明者らは、醗酵乳飲
料の凝集分離防止が従来の安定剤を添加する方法のみで
は充分に解決できるに至っていないという問題を解決す
るために鋭意検討を重ねた結果、CMCのうちの特定の
ものが、醗酵乳飲料用安定剤として好ましい特性を有
し、かつロットナンバーが異なることによる性能のバラ
ツキが小さいことを見出し、本発明を完成するに至っ
た。Means for Solving the Problems The present inventors have made intensive studies to solve the problem that the prevention of coagulation and separation of fermented milk beverages cannot be sufficiently solved only by the conventional method of adding a stabilizer. As a result of the repetition, it was found that a specific one of the CMCs had preferable characteristics as a stabilizer for fermented milk beverages, and that the variation in performance due to the different lot numbers was small, and the present invention was completed. .
【0011】すなわち、エーテル化度が0.8〜1.
2、1%(重量%、以下同様)水溶液粘度が5〜50m
Pa・s、耐酸性が0.5〜1.2であるカルボキシメ
チルセルロース塩を含有することを特徴とする醗酵乳飲
料用安定剤(請求項1)、乳蛋白原料を醗酵させて醗酵
乳飲料を製造する方法において、請求項1記載の醗酵乳
飲料用安定剤を添加することを特徴とする醗酵乳飲料の
製法(請求項2)、および請求項2において、醗酵前に
乳蛋白原料に請求項1記載の醗酵乳飲料用安定剤を添加
することを特徴とする醗酵乳飲料の製法(請求項3)に
関する。That is, the degree of etherification is 0.8-1.
2, 1% (wt%, the same applies hereinafter) aqueous solution viscosity of 5 to 50 m
A stabilizer for fermented milk drinks (Patent 1), comprising a carboxymethylcellulose salt having a Pa · s and acid resistance of 0.5 to 1.2 (Claim 1). A method for producing a fermented milk beverage, wherein the stabilizer for fermented milk beverage according to claim 1 is added to the milk protein raw material before fermentation. A method for producing a fermented milk drink, which comprises adding the stabilizer for fermented milk drink according to claim 1 (claim 3).
【0012】[0012]
【発明の実施の形態】本発明の醗酵乳飲料用安定剤は、
乳蛋白原料を醗酵させて醗酵乳飲料を製造するのに使用
される。醗酵乳飲料に前記安定剤を含有させることによ
り、醗酵乳飲料の凝集分離を充分に防止することがで
き、かつロットナンバーが異なることによる性能のバラ
ツキが小さくなるという効果がえられる。BEST MODE FOR CARRYING OUT THE INVENTION The stabilizer for fermented milk beverages of the present invention comprises:
It is used to produce fermented milk beverages by fermenting milk protein ingredients. By including the stabilizer in the fermented milk beverage, it is possible to sufficiently prevent coagulation and separation of the fermented milk beverage, and to reduce the variation in performance due to different lot numbers.
【0013】前記安定剤には、エーテル化度が0.8〜
1.2、1%水溶液粘度が5〜50mPa・s、耐酸性
が0.5〜1.2のCMCが含まれる。The stabilizer has a degree of etherification of 0.8 to 0.8.
1.2 CMC having a 1% aqueous solution viscosity of 5 to 50 mPa · s and an acid resistance of 0.5 to 1.2 is included.
【0014】前記CMCのエーテル化度が0.8未満の
ばあいには、乳蛋白の凝集分離防止効果が充分でなくな
り、1.2をこえてもこの効果は薄れる。前記CMCの
粘度は目的とする醗酵乳飲料の粘度によって決まるもの
であるが、1%水溶液粘度が5〜50mPa・sと低粘
度品が好ましい。1%水溶液粘度が5mPa・s未満の
ばあい、醗酵乳飲料の凝集分離防止効果が小さく、50
mPa・sをこえると、醗酵乳原料に添加したとき粘稠
性が大きくなり、醗酵乳飲料の製造作業性が低下する。When the degree of etherification of the CMC is less than 0.8, the effect of preventing coagulation and separation of milk protein is not sufficient, and even if it exceeds 1.2, this effect is weakened. The viscosity of the CMC is determined by the viscosity of the desired fermented milk beverage, but a low viscosity product having a 1% aqueous solution viscosity of 5 to 50 mPa · s is preferable. When the 1% aqueous solution viscosity is less than 5 mPa · s, the effect of preventing coagulation and separation of the fermented milk beverage is small,
If the viscosity exceeds mPa · s, the viscosity increases when added to the raw material of fermented milk, and the workability of producing a fermented milk beverage decreases.
【0015】また、醗酵乳は通常pH3.0〜4.5の
範囲になるので、使用するCMCが低pH域で粘度変化
してゲル化または粘度低下することは好ましくない。そ
れゆえ、低pH域で粘度変化の少ないものが好ましく、
耐酸性が0.5〜1.2の範囲であることが必要であ
る。CMCの耐酸性の評価方法については後述する。Further, since fermented milk usually has a pH in the range of 3.0 to 4.5, it is not preferable that the viscosity of the CMC used in the fermented milk changes in a low pH range to cause gelation or decrease in viscosity. Therefore, those with little change in viscosity in a low pH range are preferable,
It is necessary that the acid resistance is in the range of 0.5 to 1.2. The method for evaluating the acid resistance of CMC will be described later.
【0016】本発明で用いられるCMCは従来公知の方
法で製造され、エーテル化度、1%水溶液粘度および耐
酸性が本発明で用いられるCMCの値になるものを分別
することによってうることができる。The CMC used in the present invention is produced by a conventionally known method, and can be obtained by fractionating those whose degree of etherification, 1% aqueous solution viscosity and acid resistance are those of the CMC used in the present invention. .
【0017】前記安定剤には、前記CMCが、好ましく
は50%以上、さらには70%以上、100%以下含有
される。CMCの含有率が低すぎるばあいには、安定剤
の使用量が通常量であっても、前記CMCの有用性が充
分発揮できなくなる傾向が生じる。The stabilizer preferably contains the CMC in an amount of 50% or more, more preferably 70% or more and 100% or less. If the content of CMC is too low, there is a tendency that the usefulness of the CMC cannot be sufficiently exhibited even if the amount of the stabilizer used is an ordinary amount.
【0018】前記CMC以外に含まれうる成分として
は、ペクチン、アルギン酸ナトリウム、アルギン酸プロ
ピレングリコールエステルなどがあげられる。Components that can be included in addition to the CMC include pectin, sodium alginate, propylene glycol alginate and the like.
【0019】前記安定剤の性状としては、通常、前記C
MCおよび必要により前記CMC以外に含まれる成分が
混合された粉末状である。また、前記CMCと前記CM
C以外に含まれる成分は、醗酵乳飲料を製造する際に水
溶液にするときに、それぞれ別々に添加して安定剤を形
成してもよい。As the properties of the stabilizer, the above-mentioned C
It is in the form of a powder in which MC and, if necessary, components other than the CMC are mixed. Further, the CMC and the CM
Ingredients other than C may be separately added to form a stabilizer when an aqueous solution is produced when producing a fermented milk beverage.
【0020】本発明の醗酵乳飲料の製法における前記安
定剤の添加については、乳蛋白原料を醗酵させて醗酵乳
飲料を製造する際に、醗酵前、または醗酵中に乳蛋白原
料に添加してもよく、また、醗酵後に醗酵乳に添加して
もよく、添加の方法についてはとくに限定はないが、醗
酵前に乳蛋白原料に添加するのが、醗酵乳飲料を製造す
るときの乳蛋白の凝集分離を防止できる点から好まし
い。With respect to the addition of the stabilizer in the method for producing a fermented milk beverage of the present invention, the fermented milk protein raw material is fermented to produce a fermented milk beverage, and the fermented milk beverage is added to the milk protein raw material before or during fermentation. The fermented milk may be added to the fermented milk after fermentation, and the method of addition is not particularly limited. It is preferable because coagulation separation can be prevented.
【0021】前記乳蛋白原料を醗酵させて醗酵乳飲料を
製造する方法には特別な限定はなく、従来から行なわれ
ている方法を、とくに限定なく用いることができる。具
体的には、たとえば以下のように製造される。The method for producing a fermented milk drink by fermenting the milk protein raw material is not particularly limited, and a conventional method can be used without any particular limitation. Specifically, for example, it is manufactured as follows.
【0022】安定剤としてCMC100%品を使用する
ばあい、前記CMCを所定の脱脂粉乳に対する割合にな
るように水に溶解してCMC水溶液を調製し、えられた
CMC水溶液に、通常、50〜60℃程度で脱脂粉乳を
攪拌機を用いて溶解させて脱脂粉乳液を製造する。When a 100% CMC product is used as a stabilizer, the CMC is dissolved in water so as to have a predetermined ratio with respect to the skim milk powder to prepare a CMC aqueous solution. The skim milk powder is dissolved at about 60 ° C. using a stirrer to produce a skim milk powder.
【0023】つぎに、えられた脱脂粉乳液を通常の条件
でオートクレーブにかけて殺菌し、充分冷却したのち、
別に培養しておいた種菌(たとえば、醗酵乳菌であるL
ac・helveticusβ−1など)を加え、通
常、37℃で18〜20時間程度の乳酸菌醗酵を行ない
醗酵乳をうる。えられた醗酵乳は冷蔵庫で保存してお
く。Next, the obtained skimmed milk powder is sterilized in an autoclave under ordinary conditions, and after sufficiently cooling,
Seeds that have been separately cultured (for example, L. fermented milk bacteria)
ac.helveticus β-1), and fermented with lactic acid bacteria at 37 ° C. for about 18 to 20 hours to obtain fermented milk. Store the obtained fermented milk in the refrigerator.
【0024】一方、目的とする醗酵乳飲料の配合にした
がって砂糖、オレンジ果汁、ブドウ糖などと水との混合
物を調製したのち、通常の条件で殺菌し、20℃まで冷
却する。On the other hand, a mixture of sugar, orange juice, glucose, etc. and water is prepared according to the desired fermented milk drink, and then sterilized under ordinary conditions and cooled to 20 ° C.
【0025】つぎに、前記混合物に、乳固形分が目的と
する割合になるように前記醗酵乳を加え、均一溶液に
し、クエン酸などでpH調整し、全量を水で調整し、1
50気圧でホモジナイザーにかけて目的とする乳固形分
の醗酵乳飲料をうる。Next, the fermented milk is added to the mixture so that the milk solid content becomes a desired ratio, and the mixture is made a uniform solution, pH is adjusted with citric acid or the like, and the whole amount is adjusted with water.
A homogenizer is applied at 50 atm to obtain a fermented milk beverage having a desired milk solid content.
【0026】前記醗酵乳飲料は前記のとおり、乳製品を
乳酸菌で醗酵したものであり、糊状または液状のもので
ある。一般には、無脂乳固形分3.0%以上のものが醗
酵乳飲料とよばれ、水で3%未満にしたものは乳酸菌飲
料とよばれるが、本発明における醗酵乳飲料には両者と
も含まれる。As described above, the fermented milk drink is obtained by fermenting dairy products with lactic acid bacteria, and is in the form of paste or liquid. Generally, non-fat milk solids having a solid content of 3.0% or more are called fermented milk drinks, and those made with less than 3% of water are called lactic acid bacteria drinks. It is.
【0027】本発明における醗酵乳飲料に含まれる乳酸
菌は、生菌、殺菌のいずれでもよく、また、糖分、酸性
化剤、果汁、色素などの添加剤の種類、配合量などにも
とくに限定はない。The lactic acid bacteria contained in the fermented milk beverage of the present invention may be either live bacteria or sterilized. In addition, there are no particular restrictions on the types and amounts of additives such as sugars, acidifying agents, fruit juices and pigments. Absent.
【0028】前記安定剤の添加量は、醗酵乳原料(固形
分)に対して固形分で1〜20%、好ましくは5〜10
%の範囲である。添加量は、醗酵乳原料の種類、乳固形
分濃度、醗酵の程度、その他の添加量の種類と量、醗酵
乳飲料のpH、粘度などによって決められるべきもので
あり、狭い範囲で限定することはできない。The amount of the stabilizer to be added is 1 to 20%, preferably 5 to 10% in solid content with respect to the fermented milk raw material (solid content).
% Range. The amount to be added should be determined by the type of fermented milk raw material, milk solids content, degree of fermentation, type and amount of other added amount, pH and viscosity of fermented milk drink, etc. Can not.
【0029】このようにして製造される本発明による醗
酵乳飲料は乳蛋白の沈殿発生や長期間保存における乳蛋
白の凝集分離などによる商品価値の低下をきたすことが
ないという特徴を有するものである。The thus produced fermented milk beverage according to the present invention is characterized in that it does not cause a decrease in commercial value due to generation of sedimentation of milk protein and aggregation separation of milk protein during long-term storage. .
【0030】なお、本発明による醗酵乳飲料に、さらに
従来から添加されていたようなCMCや他の安定剤を追
添加しても何ら支障はない。The fermented milk beverage according to the present invention may be supplemented with CMC and other stabilizers which have been conventionally added without any problem.
【0031】[0031]
【実施例】つぎに本発明の醗酵乳飲料用安定剤およびそ
れを用いた醗酵乳飲料の製法を実施例に基づき詳細に説
明するが、本発明はかかる実施例のみに限定されるもの
ではない。EXAMPLES Next, the stabilizer for fermented milk drink of the present invention and the method for producing a fermented milk drink using the same will be described in detail with reference to examples, but the present invention is not limited to these examples. .
【0032】なお、実施例および比較例で評価したエー
テル化度、1%水溶液粘度、耐酸性の評価方法を以下に
示す。The methods for evaluating the degree of etherification, the viscosity of 1% aqueous solution, and the acid resistance evaluated in Examples and Comparative Examples are shown below.
【0033】(エーテル化度)CMC(4水和物)1g
を精秤し、磁製るつぼにいれ、約600℃で灰化し、灰
化により生成した酸化ナトリウムに0.1N硫酸水溶液
を過剰に加え、フェノールフタレインを指示薬として過
剰の硫酸を0.1N水酸化カリウム水溶液で逆滴定し、
式(1):(Degree of etherification) 1 g of CMC (tetrahydrate)
Is weighed and placed in a porcelain crucible, incinerated at about 600 ° C., an excess of 0.1N sulfuric acid aqueous solution is added to the sodium oxide produced by the incineration, and excess sulfuric acid is added to 0.1N water using phenolphthalein as an indicator. Back titrate with aqueous potassium oxide solution,
Equation (1):
【0034】[0034]
【数1】 (Equation 1)
【0035】から求めた。[0035]
【0036】(1%水溶液粘度)無水物換算したCMC
を1.0%含む水溶液200mlを調製し、均一水溶液
にしたのち、25℃恒温水槽に30分間おいたのちの粘
度をBM型粘度計で測定した。(1% aqueous solution viscosity) CMC converted to anhydride
Was prepared in a uniform aqueous solution, and then placed in a water bath at 25 ° C. for 30 minutes, and the viscosity was measured with a BM viscometer.
【0037】(耐酸性)無水物換算したCMCを1.1
%含む水溶液180mlを調製し、これに25%酢酸水
溶液20mlを加えて均一水溶液にした(pH約4)。(Acid resistance) The CMC calculated as anhydride was 1.1
% Aqueous solution (180 ml) was prepared, and a 25% acetic acid aqueous solution (20 ml) was added thereto to make a uniform aqueous solution (pH about 4).
【0038】前記酢酸水溶液の添加後スターラーで均一
溶液にしたのち、25℃恒温水槽に30分間おいたのち
の粘度をBM型粘度計で測定した(これを粘度値Aとす
る)。After the addition of the acetic acid aqueous solution, the mixture was made into a homogeneous solution with a stirrer, and then placed in a constant temperature water bath at 25 ° C. for 30 minutes, and the viscosity was measured with a BM type viscometer (this is referred to as viscosity value A).
【0039】えられた粘度値Aおよび前記1%水溶液粘
度を用いて、式(2):Using the obtained viscosity value A and the viscosity of the 1% aqueous solution, the formula (2):
【0040】[0040]
【数2】 (Equation 2)
【0041】から算出した。Calculated from
【0042】実施例1〜9および比較例1〜6 表1に示した特性のCMCを表1に示した脱脂粉乳に対
する割合になるように水に溶解して全量が2000gに
なるようなCMC水溶液を調製した。用いたCMCの量
は、後述する脱脂粉乳500gに対して、たとえば5%
のばあいは25g、10%のばあいは50gである。Examples 1 to 9 and Comparative Examples 1 to 6 A CMC aqueous solution in which CMC having the properties shown in Table 1 was dissolved in water so as to have a ratio to the skim milk powder shown in Table 1 so that the total amount became 2000 g. Was prepared. The amount of CMC used is, for example, 5% with respect to 500 g of skim milk powder described below.
In the case of 25%, in the case of 10%, it is 50 g.
【0043】つぎに前記2000gのCMC水溶液に5
0〜60℃で脱脂粉乳500gを攪拌機を用いて溶解さ
せ、20%脱脂粉乳液をえた。Next, 5 g of the above-mentioned 2000 g CMC aqueous solution
500 g of skim milk powder was dissolved at 0 to 60 ° C. using a stirrer to obtain a 20% skim milk powder.
【0044】前記脱脂粉乳液を1000mlの三角フラ
スコ3個に分けて移し、綿栓をして、そのうえからアル
ミホイルで覆った。The skim milk powder was transferred to three 1000 ml Erlenmeyer flasks, capped with cotton, and then covered with aluminum foil.
【0045】前記脱脂粉乳液が入った三角フラスコを1
21℃、5分間オートクレーブにかけて殺菌したのち、
流水で充分冷却した。The Erlenmeyer flask containing the skim milk powder
After sterilization in an autoclave at 21 ° C for 5 minutes,
It was cooled sufficiently with running water.
【0046】冷却後、別に培養しておいた種菌(醗酵乳
菌、Lac・helveticusβ−1)を加え、綿
栓をして、アルミホイルをかぶせたのち、37℃で18
〜20時間乳酸菌醗酵を行ない、乳固形分5〜20%の
醗酵乳をえた。えられた醗酵乳は冷蔵庫で保存した。After cooling, a separately cultured inoculum (fermented milk bacterium, Lac helveticus β-1) was added, the cotton plug was covered, and aluminum foil was covered.
Lactic acid bacteria fermentation was performed for 〜20 hours to obtain fermented milk having a milk solid content of 5 to 20%. The obtained fermented milk was stored in a refrigerator.
【0047】一方、表2の配合にしたがって砂糖、オレ
ンジ果汁、ブドウ糖、水(全量2000gになるうちの
一部)の混合物を調製したのち、90℃で10分間殺菌
し、20℃まで冷却した。On the other hand, a mixture of sugar, orange juice, glucose, and water (a part of the total amount of 2000 g) was prepared according to the composition shown in Table 2, sterilized at 90 ° C. for 10 minutes, and cooled to 20 ° C.
【0048】つぎに、前記混合物に、乳固形分が表2記
載の割合になるように醗酵乳をそれぞれ加え、均一溶液
とし、これにクエン酸を添加してpHメーターでpH
3.4〜3.7になるように調整し、全量2000gに
なるように水を加えたのち、150気圧でホモジナイザ
ーにかけ、乳固形分の異なる2種の醗酵乳飲料をえた。Next, fermented milk was added to each of the above mixtures so that the milk solid content was in the proportions shown in Table 2, thereby obtaining uniform solutions. Citric acid was added thereto, and pH was measured with a pH meter.
The mixture was adjusted to 3.4 to 3.7, water was added so that the total amount became 2000 g, and the mixture was homogenized at 150 atm to obtain two types of fermented milk drinks having different milk solid contents.
【0049】えられた醗酵乳飲料のそれぞれを100m
l容で高さ250mmの目盛りつきガラス円筒管にと
り、冷蔵庫中で5℃で14日間放置した。Each of the obtained fermented milk drinks is 100 m
The tube was placed in a glass cylinder tube having a volume of 250 mm and a height of 250 mm, and allowed to stand in a refrigerator at 5 ° C. for 14 days.
【0050】14日間経過後、それぞれのガラス円筒管
中の状態を観察し、乳蛋白の沈殿量を沈殿量mlとして
読みとった。After 14 days, the state of each glass cylinder was observed, and the amount of milk protein precipitated was read as the amount of precipitated milk.
【0051】結果を表1に示す。Table 1 shows the results.
【0052】[0052]
【表1】 [Table 1]
【0053】[0053]
【表2】 [Table 2]
【0054】実施例10〜18 水2000gに50〜60℃で脱脂粉乳500gを攪拌
機を用いて溶解させ、安定剤を含まない20%脱脂粉乳
液をえた。Examples 10 to 18 500 g of nonfat dry milk was dissolved in 2000 g of water at 50 to 60 ° C. using a stirrer to obtain a 20% nonfat dry milk without stabilizer.
【0055】前記脱脂粉乳液を1000mlの三角フラ
スコ3個に分けて移し、綿栓をして、そのうえからアル
ミホイルで覆った。The skim milk powder was divided into three 1000 ml Erlenmeyer flasks, transferred with a cotton plug, and covered with aluminum foil.
【0056】前記脱脂粉乳液が入った三角フラスコを1
21℃、5分間オートクレーブにかけて殺菌したのち、
流水で充分冷却した。The Erlenmeyer flask containing the skim powder emulsion
After sterilization in an autoclave at 21 ° C for 5 minutes,
It was cooled sufficiently with running water.
【0057】冷却後、別に培養しておいた種菌(醗酵乳
菌、Lac・helveticusβ−1)を加え、綿
栓をして、アルミホイルをかぶせたのち、37℃で18
〜20時間乳酸菌醗酵を行ない、乳固形分5〜20%の
醗酵乳をえた。えられた醗酵乳を冷蔵庫で保存した。After cooling, a separately cultured inoculum (fermented milk bacterium, Lac helveticus β-1) was added, the cotton plug was covered, and aluminum foil was covered.
Lactic acid bacteria fermentation was performed for 〜20 hours to obtain fermented milk having a milk solid content of 5 to 20%. The obtained fermented milk was stored in a refrigerator.
【0058】一方、表2の配合にしたがって砂糖、オレ
ンジ果汁、ブドウ糖、水(全量2000gになるうちの
一部)の混合物を調製したのち、90℃で10分間殺菌
し、20℃まで冷却した。On the other hand, a mixture of sugar, orange juice, glucose, and water (a part of the total amount of 2000 g) was prepared according to the composition shown in Table 2, sterilized at 90 ° C. for 10 minutes, and cooled to 20 ° C.
【0059】つぎに、前記混合物に、乳固形分が表2記
載の割合になるように醗酵乳をそれぞれ加え、均一溶液
とし、ついで、表3に示した特性のCMCを表3に示し
た脱脂粉乳に対する割合になるように溶解した。これに
クエン酸を添加してpHメーターでpH3.4〜3.7
になるように調整し、全量2000gになるように水を
加えたのち、150気圧でホモジナイザーにかけ、乳固
形分の異なる2種の醗酵乳飲料をえた。Next, fermented milk was added to the mixture so that the milk solid content was in the proportions shown in Table 2 to form uniform solutions. Then, CMC having the properties shown in Table 3 was degreased as shown in Table 3. It dissolved so that it might become the ratio with respect to milk powder. To this was added citric acid, and the pH was measured at pH 3.4 to 3.7 using a pH meter.
After adding water so that the total amount becomes 2000 g, the mixture was homogenized at 150 atm to obtain two kinds of fermented milk drinks having different milk solid contents.
【0060】なお、CMCは乳固形分1.0%、5.0
%のそれぞれのばあいについて、えられた醗酵乳の乳固
形分からもとの脱脂粉乳の量を計算して、脱脂粉乳に対
する割合が5、8、10%になるように添加した。CMC is 1.0% milk solids, 5.0%
%, The amount of the original skim milk powder was calculated from the milk solid content of the obtained fermented milk, and was added so that the ratio to the skim milk powder was 5, 8, and 10%.
【0061】えられた醗酵乳飲料のそれぞれを100m
l容で高さ250mmの目盛りつきガラス円筒管にと
り、冷蔵庫中で5℃で14日間放置した。Each of the obtained fermented milk drinks is 100 m
The tube was placed in a glass cylinder tube having a volume of 250 mm and a height of 250 mm, and allowed to stand in a refrigerator at 5 ° C. for 14 days.
【0062】14日間経過後、それぞれのガラス円筒管
中の状態を観察し、乳蛋白の沈殿量を沈殿量mlとして
読みとった。After a lapse of 14 days, the state in each of the glass cylindrical tubes was observed, and the precipitated amount of milk protein was read as the precipitated amount ml.
【0063】結果を表3に示す。Table 3 shows the results.
【0064】[0064]
【表3】 [Table 3]
【0065】[0065]
【発明の効果】本発明の安定剤を添加配合した醗酵乳飲
料は、乳蛋白の沈殿発生、凝集分離のない商品価値の高
いものとなる。The fermented milk drink to which the stabilizer of the present invention is added has a high commercial value without milk protein precipitation and aggregation separation.
Claims (3)
%水溶液粘度が5〜50mPa・s、耐酸性が0.5〜
1.2であるカルボキシメチルセルロース塩を含有する
ことを特徴とする醗酵乳飲料用安定剤。1. An etherification degree of 0.8 to 1.2, a 1% by weight aqueous solution viscosity of 5 to 50 mPa · s, and an acid resistance of 0.5 to
A stabilizer for fermented milk beverages, which comprises the carboxymethyl cellulose salt of 1.2.
造する方法において、請求項1記載の醗酵乳飲料用安定
剤を添加することを特徴とする醗酵乳飲料の製法。2. A method for producing a fermented milk beverage by fermenting a milk protein raw material, wherein the stabilizer for fermented milk beverage according to claim 1 is added.
に請求項1記載の醗酵乳飲料用安定剤を添加することを
特徴とする醗酵乳飲料の製法。3. The method for producing a fermented milk beverage according to claim 2, wherein the stabilizer for fermented milk beverage according to claim 1 is added to the milk protein raw material before fermentation.
Priority Applications (1)
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---|---|---|---|
JP15438898A JP3349091B2 (en) | 1998-06-03 | 1998-06-03 | Method for selecting carboxymethyl cellulose salt |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP15438898A JP3349091B2 (en) | 1998-06-03 | 1998-06-03 | Method for selecting carboxymethyl cellulose salt |
Publications (2)
Publication Number | Publication Date |
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JPH11346648A true JPH11346648A (en) | 1999-12-21 |
JP3349091B2 JP3349091B2 (en) | 2002-11-20 |
Family
ID=15583055
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008044533A1 (en) * | 2006-10-11 | 2008-04-17 | Meiji Dairies Corporation | Method of producing fermented milk drink or lactobacillus drink |
-
1998
- 1998-06-03 JP JP15438898A patent/JP3349091B2/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008044533A1 (en) * | 2006-10-11 | 2008-04-17 | Meiji Dairies Corporation | Method of producing fermented milk drink or lactobacillus drink |
JPWO2008044533A1 (en) * | 2006-10-11 | 2010-02-12 | 明治乳業株式会社 | Method for producing fermented milk drink or lactic acid bacteria drink |
JP5160435B2 (en) * | 2006-10-11 | 2013-03-13 | 株式会社明治 | Method for producing fermented milk drink or lactic acid bacteria drink |
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