JPH11285348A - Additive for forage for animal and the same forage - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an additive for forage for animals and to provide the same forage, both having a deodorizing effect on solid body wastes. SOLUTION: This additive for forage for animals comprises (A) a generation preventing-type deodorizing substance and (B) a absorption-type deodorizing substance, and this forage contains the above additive. The additive can inhibit bad smells of solid body wastes from industrial animals such as cattle, pigs, horses, chickens and the like and companionable animals such as dogs and cats, based on a synergistic effect both of the above deodorizing substances, immediately after and since their excretion.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to animal feed additives and animal feeds. More specifically, the present invention relates to an animal feed additive and an animal feed that enable deodorization of animal excrement and excrement and are particularly preferably used for companion animals.

[0002]

2. Description of the Related Art The number of foul odor complaints decreased significantly by 1980, but has since tended to level off.
It ranks second after noise in pollution complaints. In particular, complaints against livestock farmers are on a decreasing trend, but they are always at the top of foul odor complaints.
It is always desired to reduce the excretion of feces and urine in industrial animals such as horses and chickens (Anti-odor technology collection, 24, 1997). In recent years, the number of families who keep pets has been rapidly increasing in Japan with the increase in the elderly population and the nuclear family. In addition, since the purpose of keeping the pet of the owner has changed from guard dogs and rats to heart-based places, the status of pets has rapidly improved, and companion animals (generally referred to as companion animals, In recent years, the number of animals kept indoors as companion animals has increased, and the owner has a problem with the smell of excrement of pets. As for the number of odor complaints, the percentage of odor complaints classified as so-called urban / life style such as “personal house / apartment / dormitory” is increasing.
97). Therefore, animal feeds having a safe and effective deodorizing function for stench substances in feces are strongly desired.

[0003] In order to solve such problems, in the livestock industry, purification treatment has conventionally been performed by a water washing and incineration system, and most farmers have performed composting or sewage treatment in terms of cost. Also, deodorant for companion animals,
Deodorizing sprays and feed-added deodorizing materials have been used. However, conventional deodorant materials mainly deodorize ammonia, which is the main malodorous substance in urine, and have a deodorizing effect on hydrogen sulfide, methyl mercaptan, trimethylamine, etc., which are the main malodorous substances in feces other than ammonia. Was not enough.

[0004] The mechanism of the generation of the above-mentioned malodorous substances in the intestine is as follows. The bacterial flora in the gastrointestinal tract is composed of several hundred kinds of bacteria, and most of the animals other than ruminants are concentrated in the large intestine (10 ¹⁰ to 1 / g of digested matter).
0 ¹¹ ). In normal and healthy intestine, 99% of bacteria are anaerobic bacteria, and bacteria species present in healthy dogs and cats include lactic acid bacteria (good bacteria or useful bacteria) such as Bifidobacterium, Lactobacillus, Streptococcus, Obligate anaerobic bacteria (bad bacteria or harmful bacteria) such as Bacteroides and Clostridium; Bifidobacterium constitutes the dominant bacterium as lactic acid bacteria in bifidobacteria such as chickens and guinea pigs, and Lactobacillus constitutes the dominant bacterium in lactobacilli animals such as pigs, horses and dogs. . Organic matter entering the colon is mainly dietary fiber and undigested carbohydrates and proteins. A good bacterium partially digests such organic matter to produce short-chain fatty acids such as acetic acid, butyric acid, and propionic acid, and synthesizes vitamins and proteins. These substances are absorbed and become energy sources (utility). On the other hand, bad bacteria produce carcinogens and toxins, produce intestinal spoilage (protein fermentation, etc.), reduce sulfuric acid, reduce nitrate, etc. to produce malodorous substances such as hydrogen sulfide, ammonia, trimethylamine and methyl mercaptan. These are the main odorous substances in the excrement (toxic). The composition of the intestinal flora varies depending on the host's physiology, food, drugs, climate, environmental microorganisms, physical and mental stress, and the like. When the bacteria are reduced and the balance of the intestinal flora is disturbed in the direction of increasing bad bacteria or harmful bacteria, toxicities such as production of malodorous substances due to intestinal decay increase.

[0005] Therefore, in order to reduce the malodorous substances in the excrement, it is necessary to feed the above-mentioned environmental microorganisms, ie, useful bacteria, to the diet, or to reach the large intestine in an undigested state, and to remove these useful bacteria. By providing oligosaccharides as a nutrient source, it is possible to increase the ratio of good bacteria in the intestinal flora and decrease the ratio of bad bacteria, thereby suppressing the production of malodorous substances in the large intestine. Become. From this point of view, Japanese Patent Application Laid-Open No. 50-18264 discloses a feed additive for deodorizing excreted feces by adding useful substances such as vitamins to aerobic and anaerobic microorganisms. However, the deodorizing effect on the excreted feces immediately after excretion is not sufficient, and aerobic and anaerobic microorganisms mainly work in excreted feces and ferment the excreted feces to deodorize.
Further, as a feed additive utilizing a microorganism alone, Japanese Patent Application Laid-Open No. 9-322714 discloses an ammonia odor of feces excreted by adding lactococci and spores as main components and adding actinomycetes, filamentous fungi and yeast. A deodorant feed in which the production of nitrogen and sulfur compounds is suppressed by microorganisms when the excretion is reduced and excreted feces are left for one week is disclosed. However, this invention did not have a sufficient deodorizing effect on feces immediately after excretion.

Further, Japanese Patent Application Laid-Open No. 9-322714 discloses a feed additive which is fermented in a culture medium mainly composed of rice bran using ammonia oxidizing bacteria, nitric acid bacteria, sulfur oxidizing bacteria and cellulose decomposing bacteria. I have. However, although this invention has an effect on deodorizing ammonia and hydrogen sulfide in feces excreted by the bacteria, it has insufficient effect on trimethylamine and methyl mercaptan. In addition, it was largely due to the deodorizing effect of the microorganisms in the feces after excretion, and did not suppress the feces immediately after excretion. As described above, the invention utilizing these microorganisms is mainly intended for deodorizing industrial animals, and it is more effective to suppress deodorant microorganisms for one to two weeks after excretion than to suppress fecal odor immediately after excretion. Is intended to decompose and ferment nutrients in excreted feces to reduce the generation of malodorous substances, and to deodorize fecal odor immediately after excretion, which is a problem in living with companion animals at home. However, it was not able to show a sufficient effect.

[0007] Regarding the deodorizing effect of oligosaccharides, Japanese Patent Application Laid-Open No. 9-38184 discloses a deodorizing feed to which a mixture selected from tea husk, yeast fermented lees or mannan oligosaccharide is added. It is said that ammonia gas in feces is reduced by the combined use of (containing mannan oligosaccharide). However, it was not effective enough for deodorizing hydrogen sulfide, methyl mercaptan, trimethylamine, etc., which are the main odor substances in feces other than ammonia.

[0008] As other deodorizing materials (or deodorizing methods), zeolite, which has an odor absorbing effect and is approved as a feed additive material, and a plant deodorant having a deodorizing effect are known. Regarding the deodorizing effect of the above-mentioned zeolite, JP-A-58-190358 discloses that a feed additive obtained by adding molasses to zeolite, and JP-A-52-47484 discloses that a digestive enzyme-producing microorganism is adsorbed to zeolite. Feed additives are disclosed. The latter is said to improve the digestion of livestock and reduce the odor of manure by the synergistic effect of digestive enzymes and zeolite. However, both of them merely utilize the gas adsorption effect of zeolite to adsorb ammonia gas in the gastrointestinal tract and the effect is not strong. Further, as a vegetable deodorant, Japanese Patent Application Laid-Open No. 5-336895 discloses a vegetable deodorant made from a fermented solution of rice bran and soybean with Bacillus subtilis and an extract of carrot leaves. The deodorizing effect was not sufficient.

[0009]

As described above, the conventional feed-added deodorant materials mainly deodorize ammonia, and hydrogen sulfide and methyl mercaptan, which are the main malodorous substances in feces other than ammonia. The deodorizing effect on trimethylamine and trimethylamine was not sufficient. Further, the conventional deodorant material is such that deodorant microorganisms decompose and ferment nutrients in excreted feces over a week to two weeks after excretion to reduce the generation of malodorous substances. It did not suppress fecal odor immediately after excretion, which is a problem in living with companion animals. As described above, the conventional deodorant material did not have a sufficient deodorizing effect on excrement excreted immediately after excretion, and could not be expected to have an effect on all four major malodorous substances in excrement. The present invention has been made in view of the above-mentioned problems of the prior art, and as a result of conducting research on the deodorizing effect of excreted feces of animals, a production-suppressed deodorant and an adsorption-type deodorant have been found. Deodorant materials used in combination, especially deodorant microorganisms,
Oligosaccharides, zeolites and Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, mixed extracts of green tea and vegetables, one or more substances selected from the group consisting of Yanglong tea and Moroheiya It has been clarified that the combined deodorant material has a synergistic effect to improve the deodorizing effect on excrement and also has an effect of improving nutritional balance. Conventional deodorant materials mainly use microorganisms that have the function of regulating the intestinal flora, or types that add a single substance such as deodorant plant extracts or zeolites. No combination use of a deodorant substance of the type and an adsorption type deodorant substance has been carried out at all, and it has been found that the synergistic effect of the two makes it possible to effectively deodorize excreted feces. The present invention has been made based on such findings, and the present invention is an animal feed additive for deodorizing the odor of excreted feces of industrial animals and companion animals and improving the nutritional balance, and an animal feed containing the same. The purpose is to provide.

[0010]

The gist of the present invention to solve the above-mentioned problems is to provide an animal feed additive comprising a production-suppressing deodorant and an adsorption-type deodorant. The above-mentioned animal feed additive, wherein the production-suppressing deodorant is a deodorant microorganism and / or an oligosaccharide; and the adsorption-type deodorant is zeolite and / or Umbelliferae, and rice germ soybean fermented extract. The animal feed additive according to the above, which is at least one substance selected from the group consisting of mushroom-derived organic acid compounds, catechins, apple-derived polyphenols, green tea / vegetable mixed extract, Yanglong tea and Moroheiya; , Oligosaccharides, zeolites and Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, mixed extracts of green tea and vegetables, An animal feed additive as described above, which contains one or more substances selected from the group consisting of Yang Long tea and Moroheiya; an animal feed, comprising the animal feed additive as described in the above-mentioned items.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention has the above-mentioned constitution, and the animal feed additive of the present invention has a deodorizing property comprising at least a production-suppressing deodorant and an adsorption-type deodorant. It is a material. In the present specification, the production-suppressing deodorant refers to a deodorant substance capable of suppressing the production of offensive odors in a living body (particularly the large intestine) when ingested by an animal. The substance means a deodorant substance capable of adsorbing malodor generated in a living body (particularly, large intestine) when ingested by an animal. Examples of the production-suppressing deodorant include deodorant microorganisms and oligosaccharides. Examples of the deodorant microorganism used in the present invention include lactic acid bacteria (Bifidobacterium, Lactobacillus, Streptococcus, etc.), spore-forming lactic acid bacteria, Bacillus subtilis (Bacillus, etc.), actinomycetes (Streptomyces, etc.) and the like. Can be illustrated. Among such deodorant microorganisms, it is preferable to use Bacillus subtilis and lactic acid bacteria (including spore-forming lactic acid bacteria),
A single bacterium can be used in combination, or various germs can be used in combination.

The oligosaccharides used in the present invention include, for example, branched oligosaccharides (isomaltooligosaccharides) such as isomaltose, panose, isomalttriose, isomaltosylmaltose and isomaltetrate, kestose, Examples include fructo-oligosaccharides such as nystose and fructofuranosyl nystose, and milk oligosaccharides such as lactosucrose, and these can be used in appropriate combination.

The adsorptive deodorants used in the present invention include, for example, Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, mixed extracts of green tea and vegetables, and Yanglong. Tea, Moroheiya, zeolite and the like can be exemplified, and preferably, Umbelliferae and zeolite are used. As the above-mentioned Umbelliferae plants, for example, all Umbelliferous plants such as Ashitaba, carrot, celery, parsley, seri, honeywort, fennel, eucalypt, camellia, hanaudo, yamabofu can be used. Umbelliferae plants are compared with other plants,
It contains a large amount of polyphenols such as abietic acid, flavonoids, tannins, gallic acid, bradylin and shikonin, and it is thought that the deodorizing effect can be obtained by the complex compound forming reaction, offsetting action and masking action of these substances against malodorous substances. Can be Among the above-mentioned Umbelliferae plants, it is preferable to use Ashitaba, celery, parsley, celery, and honeywort, and they can be used in appropriate combination. More preferably, Ashitaba is used. The Apiaceae plant may be used as it is, but an aqueous extract, a powdered product thereof and the like may be used. Furthermore, as shown in Examples below, rice germ soybean fermented extract, mushroom-derived organic acid compounds, catechins, apple-derived polyphenols, green tea / vegetable mixed extract, Yanlong tea, Moroheiya are also suitable as adsorption-type deodorants. Can be used.

The zeolite used in the present invention includes:
Any zeolite having an offensive odor adsorbing property (absorbing property) can be used. Incidentally, the zeolite as is well known, the general formula W _m Z _n O _2n · sH is that of hydrous silicate represented by ₂ O (W is Na, Ca, K rarely B
a, Sr, Z is Si + Al (Si: Al> 1), s is not constant). Examples of the zeolite include natural zeolites (e.g., mesolite, fish-eye stone, chabazite, gmelinite, sodalite, pyroxene, stilbolite, nepheline, ricesite), and synthetic zeolites (e.g., A, X, Y-type zeolite, ZSM-5, etc.), vermiculite which is a quartz mineral substance, and the like can be used in combination, and a commercially available product may be simply used. In the present invention, it is suitable to use zeolite obtained by pulverizing the zeolite to a particle size of 2 mm or less, preferably 1 mm or less by means such as a mixer.

Preferred embodiments of the animal feed additive of the present invention include deodorant microorganisms, oligosaccharides, zeolites and Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, An embodiment in which one or more substances selected from the group consisting of green tea / vegetable mixed extract, Yanglong tea and Moroheiya are used in combination. By using these four components in combination, the excretory feces odor is reduced due to the synergistic effect of useful bacteria for deodorizing malodorous substances, oligosaccharides serving as nutrients for intestinal bacteria, plant deodorant materials, and zeolite having a malodor adsorbing action. It is possible to effectively deodorize, and a composition excellent in nutrition can be obtained.

In the present invention, the use ratio of the production-suppressing deodorant and the adsorption-type deodorant is not particularly limited, and may be in an appropriate range in consideration of the type of the breeding animal, the shape of excreted feces, the odor, and the like. Can be set. For example, the group consisting of the above-mentioned deodorant microorganisms, oligosaccharides, zeolites and Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, green tea / vegetable mixed extracts, Yanglong tea and Moroheiya In an embodiment in which one or more selected substances are used in combination, the deodorant microorganism and the oligosaccharide are mixed in a ratio of 1: 100 to 1: 10000 (weight ratio, in the case of a liquid component, in terms of solid content, the same applies hereinafter), preferably It is blended at a ratio of 1: 1000, and is selected from the group consisting of Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, green tea / vegetable mixed extract, Yanlong tea and Moroheiya. 1: 4 to 4:
1, preferably 1: 2 to 2: 1, more preferably 1: 1
And the former and the latter in a ratio of 1: 4 to 4: 1,
It is preferably blended at a ratio of 1: 2 to 2: 1, more preferably 1: 1.

The feed additive of the present invention can be in any suitable form, but is generally in the form of liquid, paste, powder, granules or granules. The feed additive in such a form may be prepared according to a conventional method.For example, after extracting the extract by boiling the leaf of a certain Umbelliferae plant, the extract obtained by pressing and filtering is added to the deodorant microorganism. , Oligosaccharides and zeolites are mixed and concentrated to obtain a liquid or pasty feed. In addition, the extract is freeze-dried according to a conventional method, and powdered feed is added by mixing deodorant microorganisms, oligosaccharides (powder), and zeolite with a powder of the Umbelliferae extract which is pulverized by a mixer or the like. Things are obtained.
Granular or granular feed can be obtained by processing the powdered animal feed additive with a molding machine after adding an auxiliary as necessary. In addition, in the case of rice germ soybean fermented extract, which is an adsorption type deodorant, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, green tea / vegetable mixed extract, Yanglong tea and Moroheiya, the above-mentioned Apiaceae plant extract is also used. An animal feed additive can be prepared according to the above-mentioned case.

The animal feed additive of the present invention can be used as it is as an animal feed additive. However, other nutrients such as sugar alcohols other than oligosaccharides, uronic acids, amino sugars and the like can be used. Polysaccharides such as sugars and mannan, plant extracts and powders other than Umbelliferae, animal fats and oils, vegetable proteins (eg, soy protein), mineral components (eg, calcium, magnesium, zinc, iron, copper, etc.), Vitamins (for example, vitamin A, vitamin B group, vitamin E
Etc.), a quality improving agent (for example, an antioxidant, a preservative, a coloring agent, etc.), an excipient and the like may be mixed as an animal feed additive. In such a method of use, the amount of the animal feed additive of the present invention can be appropriately adjusted according to the desired deodorizing effect on feces, but it is 10% (% by weight) based on the total amount of the raw material mixture. In the following, the odor of the excrement can be reduced, and is generally about 1 to 5% by weight.

As shown in the test examples described below, the animal feed additive of the present invention is an excellent animal feed additive which significantly reduces malodor of excreted feces of companion animals and industrial animals and improves nutritional value. To provide things. In particular, a preferred form of the animal feed additive of the present invention is a lactic acid bacterium having an action of decomposing malodorous substances in the intestinal tract, a deodorant microorganism such as Bacillus subtilis, an oligosaccharide serving as a nutrient source for intestinal bacteria, a deodorant action. Acetaceae plant, fermented rice germ soybean extract, mushroom-derived organic acid compounds, catechins, apple-derived polyphenols, green tea / vegetable mixed extract, one or more substances selected from the group consisting of Yanlong tea and Moroheiya When zeolite with is used together, due to their synergistic effect, compared with the case of using each alone, cattle,
It is possible to reduce the bad smell of excreted feces of industrial animals such as pigs, horses and chickens, and companion animals such as dogs and cats, and it is an excellent animal feed additive that further improves nutritional value. Furthermore,
A mixture of lactic acid bacteria (Lactobacillus coagulans) and Bacillus subtilis (Bacillus subtilis) as deodorant microorganisms,
In addition, milk oligosaccharides, which have the effect of improving the intestinal environment by selectively increasing useful bacteria in the intestine and improving the convenience such as loosening of stool as oligosaccharides, and Ashitaba as a deodorant plant. The animal feed additive of the present invention using the compound can remarkably reduce the bad odor generated by excrement and becomes an excellent animal feed additive which further improves the nutritional value.

The animal feed additive of the present invention can be used by adding to the feed of a domestic animal, but may be fed to a domestic animal as a feed containing the animal feed additive of the present invention. The animal feed of the present invention is an animal feed of such a form, and contains the animal feed additive of the present invention. The animal feed of the present invention can be prepared according to a conventional method for producing animal feed, except that the animal feed additive of the present invention is added to the raw materials. The amount of the animal feed additive can be appropriately adjusted depending on the animal species to be fed, the amount of feed, and the like, but is generally about 15% or less, preferably about 1 to 10% in the animal feed. .

[0021]

The animal feed additive and animal feed of the present invention have the following effects. The animal feed additive of the present invention contains a production-suppressing deodorant and an adsorption-type deodorant, and due to their synergistic effect, industrial animals such as cows, pigs, horses, chickens, dogs and cats. It is possible to suppress the bad smell of excrement of the companion animal immediately after excretion. In particular, deodorant microorganisms, oligosaccharides, zeolites and Apiaceae plants, rice germ soybean fermented extract, mushroom-derived organic acid compounds, catechins, which are preferred embodiments of the present invention,
When combined with one or more substances selected from the group consisting of apple-derived polyphenols, green tea / vegetable mixed extract, Yanglong tea and Moroheiya, it is a nutrient source for useful bacteria that decompose and deodorize malodorous substances and enteric bacteria. Due to the synergistic effect of oligosaccharides, vegetable deodorant materials and zeolite that has a bad odor-adsorbing effect, compared to the case of using each alone, cattle, pigs,
It is possible to remarkably reduce the bad smell generated by excrement of feces of industrial animals such as horses and chickens and companion animals such as dogs and cats. In addition, the moisture adsorption effect of the porous zeolite in the intestinal tract, and the antioxidant properties of Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, green tea / vegetable mixed extract, Yanglong tea and Moroheiya Due to the diarrhea action and the action of increasing the number of useful bacteria of milk oligosaccharides, it is possible to improve stool such as defecation frequency and loose stool. In addition, abundant vitamins and minerals of Umbelliferae, rice germ soybean fermented extract, mushroom-derived organic acid compounds, catechins, apple-derived polyphenols, green tea / vegetable mixed extract, yanglong tea and moloheiya produce industrial and companion animals. It is an animal feed additive that is excellent in replenishing nutrients that tend to be deficient and is useful for maintaining the health of industrial animals and companion animals. The animal feed of the present invention contains the above-described animal feed additive having excellent deodorizing properties, and by feeding the feed, suppresses the bad smell of the excrement of the breeding animal immediately after excretion. Can be.

[0022]

The present invention will be described below in detail with reference to examples and test examples, but the present invention is not limited to these examples. Production Example 1 (Deodorizing microorganism) Lactic acid bacteria (Lactobacillus coagulans) powder and Bacillus subtilis (Bacillus subtilis) powder were mixed at a ratio of 1: 1 to obtain a mixture. Production Example 2 (milk oligosaccharide) Lactose and sucrose were used as raw materials and were obtained by a transglycosylation reaction of β-fructofuranosidase.

Production Example 3 (Ashitaba) After collection, washed fresh Ashitaba leaves were treated at 100 ° C. and 30 ° C.
The extract was extracted, extracted, and then squeezed and filtered to obtain an Ashitaba extract. Furthermore, after removing Ashitaba residue by centrifugation, the supernatant was sterilized by boiling at 90 ° C. for 30 minutes, and pulverized by a spray drier to obtain Ashitaba extract powder.

Production Example 4 (Zeolite) Meteorite, fish-eye stone, chabazite, gumelinite, sodaite,
A mixture of pyroxene, bazite, turbidite and ricesite was crushed with a mixer to obtain a zeolite powder having a particle size of 1 mm or less.

Example 1 (Deodorant mixture) The deodorant microorganisms, milk oligosaccharides, ashitaba extract powder and zeolite powder obtained in Production Examples 1, 2, 3 and 4 were mixed at a ratio of 1: 1000: 500: 500. To obtain the animal feed additive of the present invention.

Example 2 (Jerky for dogs) Meat raw material (one or two of the group consisting of pork, chicken and beef)
Combination of more than two kinds; 38.2 parts per 100 parts by weight of the kneaded raw material mixture) and pork back fat (2.5 parts)
With a meat grinder or a silent cutter, the meat is crushed into a piece of meat having a diameter of about 10 mm or less, and ice (19.0 parts), vegetable protein (32.3 parts), bone meal (2.7 parts), salt (0 .4
Parts), polymerized phosphate (0.2 part) and sugars and seasonings (2.
2 parts), and the product of the present invention prepared in Example 1 was further added to 2.
A paste was prepared at a ratio of 5%. Next, this was molded into a stick shape by a filling molding machine, dried by heating, and cut into about 3 cm to produce an animal feed.

Example 3 (cat jerky) Meat raw material (one or two of the group consisting of pork, chicken and beef)
Combination of more than two kinds; 36.2 parts per 100 parts by weight of the kneaded raw material mixture) and pork back fat (2.0 parts)
Using a meat grinder or a silent cutter, the meat is crushed into a piece of meat having a diameter of about 10 mm or less, and ice (17.3 parts), vegetable protein (32.3 parts), bone meal (2.2 parts), salt (0%) are added. .4
Parts), polymerized phosphate (0.2 part) and sugars and seasonings (6.
9 parts), and the product of the present invention prepared in Example 1 was further added to 2.
A paste was prepared at a ratio of 5%. Next, this was molded into a stick shape by a filling molding machine, dried by heating, and cut into about 3 cm to produce an animal feed.

Example 4 (Vitamin and mineral-enriched feed) Meat raw material (one or two of the group consisting of pork, chicken and beef)
Combination of more than two kinds; 36.2 parts per 100 parts by weight of the kneaded raw material mixture) and pork back fat (2.0 parts)
With a meat grinder or a silent cutter, the meat is crushed into a piece of meat having a diameter of about 10 mm or less, and ice (16.9 parts), vegetable protein (32.3 parts), bone meal (2.2 parts), salt (0%) are added. .4
Parts), multivitamin (0.4 parts), polymerized phosphate (0.4 parts)
2 parts), sugars and seasonings, etc. (6.6 parts) and vitamin mineral premix (0.3 part).
A paste was prepared by mixing the product of the present invention prepared in the above at a ratio of 2.5%. Next, this was molded into a stick shape by a filling molding machine, dried by heating, and cut into about 3 cm to produce an animal feed.

Example 5 (Deodorant mixture) The deodorant microorganisms, isomaltooligosaccharides, parsley extract powder and zeolite powder obtained in Production Examples 1, 2, 3 and 4 were mixed at a ratio of 2: 750: 800: 1200. To obtain the animal feed additive of the present invention.

Example 6 (Deodorant mixture) In place of the Ashitaba extract used in Example 1, one of the following commercially available deodorants (a) to (g) was used. Deodorant microorganism), Production Example 2 (milk oligosaccharide),
One of the following commercially available deodorants (a) to (g) and zeolite powder are blended in a ratio of 1: 1000: 2000: 1000,
An animal feed additive of the present invention was obtained. (a) Rice germ soybean fermented extract (powder) (b) Mushroom-derived organic acid compound (c) Green tea-derived catechins (d) Apple-derived polyphenol (e) Green tea / vegetable mixed extract (powder) (f) Yanglon tea (g ) Moroheiya (powder)

Example 7 An animal feed (for dogs) was prepared in the same manner as in Example 2, except that the product of Example 6 was used in place of the product of Example 1 at a rate of 3.0%. Jerky) was prepared.

Example 8 An animal feed (for cats) was prepared in the same manner as in Example 3 except that the product of Example 6 was used in place of the product of Example 1 at a rate of 3.0%. Jerky) was prepared.

Comparative Example 1 (Deodorant microorganism, feed supplemented with milk oligosaccharide) Production examples 1 and 2 in place of the product of the present invention prepared in Example 1
Was blended at a ratio of 2.5% by weight, and thereafter, a paste was prepared in the same manner as in Example 2. Next, this was molded into a stick shape by a filling molding machine, dried by heating, and cut into about 3 cm to produce an animal feed.

Comparative Example 2 (Ashitaba, zeolite-added feed) In place of the product of the present invention prepared in Example 1, Production Examples 3 and 4
Was blended at a ratio of 2.5% by weight, and then the same mixture and method as in Example 2 were used to prepare ground meat. Next, this is molded into a stick shape by a filling molding machine,
After heating and drying, it was cut into about 3 cm to produce animal feed.

Comparative Example 3 (feed without added deodorant) Meat raw material (one or two of the group consisting of pork, chicken and beef)
Combination of more than one kind; 40.7 parts per 100 parts by weight of the kneaded raw material mixture) and pork back fat (2.5 parts)
With a meat grinder or a silent cutter, the meat is crushed into a piece of meat having a diameter of about 10 mm or less, and ice (19.0 parts), vegetable protein (32.3 parts), bone meal (2.7 parts), salt (0 .4
Parts), polymerized phosphate (0.2 part) and sugars and seasonings (2.
2 parts) was prepared. Next, this is molded into a stick shape by a filling molding machine, and after heating and drying, about 3
It was cut into cm to produce animal feed.

Test Example 1 (In Vitro Deodorizing Test; Hydrogen Sulfide) The deodorizing effect of each of the deodorizing substances described in Production Examples 3, 4, and 6 and a mixture thereof on hydrogen sulfide by the following method. Was tested. A. Test method By bubbling H ₂ S gas generated by heating hydrogen sulfide (H ₂ S) powder into artificial intestinal fluid (amylase, lipase, trypsin, bile powder, containing NaCl and NaNO ₃ , pH 7.3) the H ₂ S solution was prepared. 1 ml of this H ₂ S solution and 1 g of the deodorant substances (a) to (g) described in Production Examples 3, 4 or 6 were added to the reaction tank, and reacted at 37 ° C. for 30 minutes. H ₂ S concentration in the headspace was measured using a Kitagawa detector tube. Similarly, 1 ml of the above H ₂ S solution and two of the group consisting of the deodorizing substances (a) to (g) described in Production Examples 3, 4 and 6 (each 0.5 g each) , Total 1
g) was added to the reaction vessel and reacted at 37 ° C. for 30 minutes.
The H ₂ S concentration in the head space of the reaction tank was measured using a Kitagawa detector tube, and the presence or absence of a synergistic effect of the combined use of these deodorants on the deodorant effect was examined.

B. Results The test results are shown in FIGS. 1A and 1B. The vertical axis in the figure is H ₂ S
In the headspace when only 1 ml of solution is placed in the reaction tank
It represents the concentration of H ₂ S as 100, represents the headspace concentration of H ₂ S for each test group as a relative value. The following is clear from the results of FIG. 1A. Control (H ₂ S
Production Example 3 (Ashitaba) and Production Example 4 (zeolite) [p <0.01 for more than 2 products]
(a) Rice germ soybean fermented extract, (b) mushroom-derived organic acid compound, (c) catechins, (d) apple-derived polyphenol, (e) green tea / vegetable mixed extract, (f) Yanglong tea, (g) Moroheiya [For these, p <0.05] reduced H ₂ S concentration. The following is clear from the results of FIG. 1B. Production Example 3 (Ashitaba) or Production Example 4 (Zeolite)
And mixtures with other deodorants reduced H ₂ S concentration. In particular, the mixture of Production Example 3 (Ashitaba) and Production Example 4 (zeolite) significantly reduced the H ₂ S concentration (p <0.01), and a remarkable synergistic effect was observed.

Test Example 2 (Deodorizing Test Using Dog) The deodorizing effect of the animal feed prepared in Examples 2, 7 and Comparative Examples 1, 2, and 3 was tested by the following method. A. Test method Test animals 10 beagle dogs (sex and gender, average weight 11.5 kg,
(Average 2.6 years old) and were single bred. Materials and Measurement Methods The animal feeds prepared in Examples 2 and 7 and Comparative Examples 1, 2, and 3 were fed for 4 weeks in an amount corresponding to the daily required metabolic calorie amount of each test animal. Manure excreted in the last week of the test was collected in a plastic bag with a zipper, and the concentrations of hydrogen sulfide, ammonia, trimethylamine and methyl mercaptan in the headspace were measured using a Kitagawa detector tube.

B. Results A part of the test results is shown in FIG. The horizontal axis of the figure indicates the concentration (ppm) of each malodorous substance (hydrogen sulfide, ammonia, trimethylamine and methyl mercaptan). The following is clear from the results of FIG. Compared with the feed of Comparative Example 3, the feeds of Comparative Examples 1 and 2 were able to reduce the concentrations of up to three types of malodorous substances. On the other hand, the feed of Example 2 was able to reduce the concentration of all offensive odor substances (p <0.0
1). Similarly, the feed of Example 7 was able to reduce the concentration of all the malodorous substances (p <0.01). Thus, it became clear that the feed of the present invention has an extremely excellent deodorizing effect.

Test Example 3 (Deodorizing Test Using Cat) The deodorizing effect of the feed additive for companion animals prepared in Examples 3, 8 and Comparative Examples 1, 2, and 3 was tested by the following method. A. Test method Test animals 10 hybrid cats (sex and gender, weight average 2.5 kg, average 2.
3 years old). Materials and Measurement Method A test was performed in the same manner as in Test Example 2. B. Results A part of the test results is shown in FIG. The following is clear from the results of FIG. Compared with the feed of Comparative Example 3, the feeds of Comparative Examples 1 and 2 were able to reduce the concentrations of up to three types of malodorous substances. On the other hand, the feed of Example 3 was able to reduce the concentration of all malodorous substances (p <0.01). Similarly, the feed of Example 8 was able to reduce the concentration of all malodorous substances (p <0.01). Thus, it became clear that the feed of the present invention has an extremely excellent deodorizing effect.

Test Example 4 (Sensory Test Using Dog Manure) A sensory test of each excreted manure sampled in Test Example 2 was performed by the following method. A. Test method 30 healthy adults (17 men, 13 women, age 20)
(~ 55 years old) as a panelist, a certain amount of each excrement collected in Test Example 2 was collected in a plastic bag, an odor paper was inserted for 1 minute, taken out, and the odor was measured by a six-stage odor intensity display method by an odor prevention method. evaluated. The details of the display method are as follows. Odor intensity 0: no odor Odor intensity 1: odor that can be detected at last (detection threshold) Odor intensity 2: weak odor to know what odor (cognition threshold) Odor intensity 3: odor that can be easily detected Odor intensity 4: strong odor Odor intensity 5: Strong smell B. Results Table 1 shows the test results (average values). From the results in Table 1, the following became clear. The odor intensity of the excreted manure of the feeds of Comparative Examples 1 and 2 was 2.38 and 2.86, respectively.
(p <0.05), but the odor intensity of the excreted manure of the feed of Example 2 was 1.25 (p <0.01). Thus, it was also organoleptically revealed that the feed of the present invention has an extremely excellent deodorizing effect.

[0042]

[Table 1]

Test Example 5 (Sensory test using feces of cats) A sensory test of each excreted manure collected in Test Example 3 was performed by the following method. A. Test Method A test was performed in the same manner as in Test Example 4. B. Results The test results (average values) are shown in Table 2. From the results in Table 2, the following became clear. The odor intensity of the excreted manure of the feeds of Comparative Examples 1 and 2 was 2.98, 2.79 (p <0.05), respectively.
However, the odor intensity of the excreted manure of the feed of Example 3 was 1.67 (p <0.01). Thus, it was also organoleptically revealed that the feed of the present invention has an extremely excellent deodorizing effect.

[0044]

[Table 2]

Test Example 6 (Analysis Test) The nutritional analysis of the product of the present invention prepared in Example 4 and the comparative product prepared in Comparative Example 3 was carried out in accordance with the feed analysis standard determined by the Director of the Ministry of Agriculture, Forestry and Fisheries, Livestock and Agriculture Bureau. Table 3 shows the analysis results of minerals and vitamins. Minerals and vitamins that are deficient in dogs or cats and are known to have deficiency disorders include magnesium, iron, copper, zinc, selenium, vitamin A and vitamin B.
There are groups. From the results of the analysis, the feed of Comparative Example 3 was poor in minerals and vitamins in all items measured as compared with the feed of Example 4, and iron, vitamin A, vitamin B1, and vitamin B2 were determined by the Pet Food Fair Trade Council. Fail to meet the recommended NRC (National Academy of Sciences and US Research and Research Council) nutrition standards for dock and cat foods, albeit slightly;
Nutrient supplementation is considered insufficient. However, Example 4
By mixing the product of the present invention as described above, the nutritional value is improved, and iron, vitamin A, vitamin B1, and vitamin B2, which did not meet the nutritional standards in Comparative Example 3 in which the product of the present invention was not mixed, satisfy the nutritional standards. It was found that nutrient supplementation could be improved.

[0046]

[Table 3]

[Brief description of the drawings]

FIG. 1 shows Production Examples 3, 4 and Example 6 in Test Example 1.
FIG. 3 is a diagram showing the deodorizing effect of each deodorant substance shown in FIG.

FIG. 2 shows Example 2 and Comparative Example 1 in Test Example 2,
It is a figure which shows the deodorizing effect of the animal feed prepared in 2 and 3.

FIG. 3 shows Example 3 and Comparative Example 1 in Test Example 3.
It is a figure which shows the deodorizing effect of the feed additive for companion animals produced in 2 and 3.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Masakatsu Shioya 1504 Shirai-cho, Inba-gun, Chiba

Claims (5)

[Claims] An animal feed additive comprising a production-suppressing deodorant and an adsorption-type deodorant. 2. The animal feed additive according to claim 1, wherein the production-suppressing deodorant is a deodorant microorganism and / or an oligosaccharide. 3. The adsorption-type deodorant substance is selected from the group consisting of Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, green tea / vegetable mixed extract, Yanlong tea and Moroheiya. Animal feed additive according to claim 1, which is one or more selected substances and / or zeolites. 4. Deodorant microorganisms, oligosaccharides, zeolites and Umbelliferae, fermented rice germ soybean extract, organic acid compounds derived from mushrooms, catechins, polyphenols derived from apples, green tea / vegetable mixed extract, Yanglong tea and Moroheiya The animal feed additive according to claim 1, comprising one or more substances selected from the group. 5. An animal feed comprising the animal feed additive according to claim 1. Description: