JPH1123583A - Reagent dispensing device and reagent syringe used therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To simplify construction to reduce manufacturing cost without using a valve such as a three-way valve on the position where reagent can exist, restrain consumption of washing water and dummy reagent to reduce running cost, and further shorten a dispensing cycle. SOLUTION: This reagent dispensing device is provided with a plurality of reagent syringes 10, 10,... respectively filled with a plurality of reagents, a plurality of reagent lines 30, 30,... leading the reagents in the respective reagent syringes to respective target positions, a distributor 51 distributing deaerated water to the respective reagent syringes, and a deaerated water syringe 44 feeding target quantities of deaerated water to a plurality of the reagent syringes. The reagent syringe 10 is provided with a syringe casing 11 formed with a reagent discharge port 13 and a deaerated water inflow port 17, and a piston 20 partitioning off the interior of the syringe casing 11 into two chambers, a reagent chamber 19 on the reagent discharge port side and a deaerated water chamber 18 on the deaerated water inflow port side, and moving in the syringe casing 11.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a reagent dispensing device for dispensing a plurality of reagents to target positions, and a reagent syringe used for the same.

[0002]

2. Description of the Related Art As the number of specimens has increased in recent years, an automatic analyzer having a high processing capability has been demanded, and a parallel system in which a plurality of small devices are arranged has been adopted. However, the parallel method doubles the number of components and is not a good solution in terms of cost and reliability.

In an automatic analyzer, regarding a reagent dispensing device for dispensing a plurality of reagents, the conventional method is roughly classified into the following.
It is divided into pipetting method and dispenser method.

The pipetting method is disclosed in, for example,
As described in JP-A-2-44663,
For a plurality of reagents, use one nozzle, one syringe, etc., aspirate the target reagent from this nozzle into the syringe, etc., and then move the nozzle to the target location to remove the reagent in the syringe, etc. This is a method of discharging from a nozzle. In this method, to prevent contamination between reagents,
Once a certain reagent has been dispensed, it is necessary to wash the nozzle, the syringe, etc. with washing water before dispensing another reagent. Therefore, there is a disadvantage that a large amount of pure water is consumed. Further, each time the nozzle is reciprocated from the reagent suction position to the reagent discharge position, a washing operation is performed, so that the dispensing cycle time becomes longer. Another drawback is that a relatively complicated and large robot or the like is required for the reciprocating movement of the nozzle. Furthermore, in order to minimize the thinning of the reagent sucked into the syringe due to the washing water used in the washing process, it is necessary to suck a reagent dummy, and there is a drawback that the reagent is wasted.

On the other hand, the dispenser system is disclosed in, for example, Japanese Patent Application Laid-Open No. Sho 60-2250645 and Japanese Patent Publication No. 1-25095.
As described in Japanese Patent Application Laid-Open No. HEI 4-160367, nozzles for the number of reagents and reagent syringes (or reagent containers) for the number of reagents are used for a plurality of reagents. In this method, a dedicated nozzle and a reagent syringe (or reagent container) are used for each reagent. In this method, there is no contamination between reagents, and if each nozzle is placed at the corresponding reagent discharge position, there is no need to move each nozzle, so the dispensing cycle time is short, and a robot for nozzle movement, etc. Is also unnecessary, and many disadvantages of the pipetting method can be eliminated.

Meanwhile, in each of the conventional techniques of the dispenser method, the one described in Japanese Patent Application Laid-Open No. 60-2250645 discloses a method in which a plurality of reagent containers, a number of reagent nozzles corresponding to the number of reagent containers, and a number of reagent containers. It has a three-way valve (three-way flow path switch) and one reagent syringe. The three openings of the three-way valve are respectively connected to a corresponding reagent container, a corresponding reagent nozzle, and a reagent syringe. This reagent dispensing device operates the reagent syringe, sucks up the reagent from the target reagent container via the three-way valve corresponding to the target reagent container, and then again operates the reagent syringe in the reverse direction, The sucked reagent is discharged from the reagent nozzle via the three-way valve, that is, the reagent is switched back and discharged.

[0007] Japanese Patent Publication No. 1-26509 discloses a plurality of reagent containers, a number of reagent nozzles corresponding to the number of the reagent containers, and a three-way valve (three-way passage switching) corresponding to the number of the reagent containers. ), A multiway flow switching valve, and one reagent syringe. Each opening of the multiway flow switching valve is connected to one opening of each three-way valve and a reagent syringe, respectively, and the three openings of the three-way valve are respectively corresponding reagent container, corresponding reagent nozzle, and multiway flow switching. It is connected to the corresponding opening of the valve. This reagent dispensing device operates a reagent syringe to draw up a reagent from a target reagent container via a three-way valve and a multi-way flow switching valve corresponding to the target reagent container, and then moves the reagent syringe in a reverse direction. Let it work,
The sucked-up reagent is discharged from the reagent nozzle via the three-way valve. That is, in this conventional technique, the reagent is switched back and discharged.

Japanese Patent Application Laid-Open No. 4-160367 discloses a plurality of reagent containers, a reagent syringe provided in each reagent container, and a three-way flow passage provided in each reagent syringe. It comprises a switch, reagent nozzles for the number of reagent containers, and a syringe and flow path switch drive mechanism for the number of reagent containers. The three openings of the three-way flow switch are respectively connected to the inside of the reagent syringe, the outside of the reagent syringe, inside the reagent container, and the corresponding reagent nozzle. The reagent dispensing apparatus drives a drive mechanism corresponding to a target reagent container, operates a reagent syringe and a three-way flow switch, and returns a three-way flow switch provided in the target reagent syringe. , After sucking the reagent from the reagent container into the reagent syringe, operating the reagent syringe and the three-way flow switch in the opposite direction, and discharging the reagent in the reagent syringe from the nozzle via the three-way flow switch It is.

[0009]

As described above, the conventional dispenser-type reagent dispensing apparatus solves many of the drawbacks of the pipetting method. A three-way switching valve or a three-way switching device is provided in the reagent line. This three-way flow valve has a complicated mechanism and is provided in a reagent line, compared with a normal two-way valve, so that it is required to have chemical resistance and abrasion resistance to various reagents. In addition, it becomes very expensive, and when reagent crystals are formed, malfunction may occur. Further, JP-A-4-160367
The dispenser-type reagent dispensing device described in the above publication requires a reagent syringe for the number of reagent containers and a drive mechanism for the number of reagent containers, in addition to the plurality of reagent containers. That is, the conventional dispenser-type reagent dispensing apparatus has a problem that relatively expensive components are used or the entire configuration is relatively complicated, and the manufacturing cost is increased.

The dispenser-type reagent dispensing apparatus described in Japanese Patent Application Laid-Open No. Sho 60-2250645 and Japanese Patent Publication No. 1-25099 requires a relatively large amount of reagent since each reagent line must be washed with washing water. In addition to the consumption of pure water, the use of a reagent dummy increases the running cost, and further requires a washing step, so that the cycle time of dispensing becomes relatively long.

The present invention has been made in view of such conventional problems, and aims to reduce the manufacturing cost and the cycle time of dispensing while eliminating the drawbacks of the pipetting method. Reagent dispensing device, and
An object is to provide a reagent syringe used for this.

[0012]

A first reagent dispensing device for achieving the above object is provided with a plurality of reagent syringes each filled with a plurality of reagents, and for each of the plurality of reagent syringes, A distribution having a plurality of reagent lines for guiding a reagent in the reagent syringe to a target position, a suction port for sucking a working fluid, and a plurality of discharge ports respectively connected to the plurality of reagent syringes and discharging the working fluid. Via a dispenser, a plurality of the reagent syringes,
A working fluid syringe for supplying the working fluid in a target amount; a plurality of working fluid branch lines respectively connecting the plurality of discharge ports and the plurality of reagent syringes of the distributor; and a plurality of the working fluid branch lines. A plurality of on-off valves provided, wherein the plurality of reagent syringes,
A syringe casing in which a reagent outlet and a working fluid inlet are formed, and the inside of the syringe casing is partitioned into two chambers: a reagent chamber on the reagent outlet side and a working fluid chamber on the working fluid inlet side, A partition member that moves in the syringe casing or deforms in the syringe casing to change the volume of the two chambers, the reagent discharge port is connected to the reagent line, and the working fluid inlet is the It is characterized by being connected to a fluid branch line.

In a second reagent dispensing apparatus for achieving the above object, in the first reagent dispensing apparatus, the partition member has an outer peripheral surface on an inner peripheral surface of the reagent syringe casing. It is a piston that moves in the reagent syringe casing while being in contact therewith.

[0014] The second reagent dispensing device may be configured such that the cross-sectional area of the piston is Sp, and the piston moves and the reagent is discharged from the reagent discharge port of the reagent syringe casing. When the pressure in the reagent chamber is Po and the frictional force between the outer peripheral surface of the piston and the inner peripheral surface of the syringe casing when the piston is moving is Fp, the relationship of 5 × Fp <Sp × Po is established. Is preferred.

In the second reagent dispensing apparatus, the piston may have an outer diameter smaller than the inner diameter of the reagent syringe casing, and an outer periphery of the piston main body on the reagent chamber side and the working fluid chamber side. Surfaces of the syringe casing, the outer peripheral surface of the piston body, the seal portion on the reagent chamber side, and the seal portion on the working fluid chamber side. The space may be filled with grease.

In the second reagent dispensing apparatus, the reagent syringe is arranged such that the reagent discharge port is located upward and the working fluid inlet is located downward, and the moving direction of the piston is , The vertical direction, the piston has
A fin may be provided on the outer peripheral surface or a lower portion thereof, and the fin gradually widens toward the lower side. The tip of the fin may be in contact with the inner peripheral surface of the reagent syringe casing and may serve as a seal between the reagent chamber and the working fluid chamber.

Further, the second reagent dispensing apparatus includes a detecting means for detecting a movement amount of the piston of the reagent syringe, and an output from the detecting means for detecting a discharge amount of the reagent, a remaining amount of the reagent, It is preferable to include a conversion unit for converting the number of times that the reagent can be ejected into at least one of the number, and a display unit for displaying the conversion result.

According to a third reagent dispensing apparatus for achieving the above object, in the first reagent dispensing apparatus, the partition member is a flexible, bag-shaped sheet. The opening edge of the bag-shaped sheet is adhered to the inner peripheral surface of the reagent syringe casing.

Here, the third reagent dispensing device includes sheet breakage means for breaking a part of the sheet when the bag-shaped sheet is deformed and the volume of the reagent chamber is minimized. May be.

The above first, second or third reagent dispensing apparatus comprises a pressure detecting means for detecting the pressure in the working fluid syringe, and operating the working fluid syringe to provide a plurality of reagent syringes. When the reagent in the target fluid syringe is discharged from the target reagent syringe and the pressure in the working fluid syringe detected by the pressure detecting means becomes equal to or higher than a predetermined value, the reagent in the target reagent syringe is discharged. Display means for displaying that the data has expired.

In the above-described first, second or third reagent dispensing apparatus, the distributor has a discharge port located above the suction port and the discharge port of the distributor. A working fluid discharge line is connected to the discharge port of the distributor,
It is preferable that an on-off valve is provided in the working fluid discharge line. In this case, it is preferable that the ends of the working fluid discharge line are located at the same height as the ends of the plurality of reagent lines.

The first reagent syringe used in the reagent dispensing device for achieving the above object has a hollow cylindrical shape, and has a reagent outlet at one end and a reagent outlet at the other end. A syringe casing body having an opening formed therein, and a piston that partitions the inside of the syringe casing body into a reagent chamber on the reagent discharge port side and a working fluid chamber on the opening side, and moves in the syringe casing body. , A reagent filled in the reagent chamber, and the piston,
A cylindrical piston main body that fits in the syringe casing, a seal formed on the outer periphery of the piston main body and in close contact with the inner peripheral surface of the syringe casing main body, and a seal formed on an end of the piston main body and outside the seal main body. The syringe casing main body has a piston main body moving area in which the piston main body can move between the reagent discharge port and the opening. An inner diameter is smaller than an inner diameter of the piston main body movement region, and is formed to have a size that can be closed by a stopper portion of the piston.

The second reagent syringe used in the reagent dispensing apparatus for achieving the above object has a hollow cylindrical shape, and has a reagent discharge port formed at one end and a reagent discharge port formed at the other end. A syringe casing body having an opening formed therein, a flexible bag-shaped body, an opening edge of the bag is adhered to an inner peripheral surface of the syringe casing body, and the inside of the syringe casing body is connected to the reagent discharge port side. A sheet partitioning into two chambers, a reagent chamber and a working fluid chamber on the opening side, a reagent filled in the reagent chamber, and a stopper closing an opening of the syringe casing body and forming a working fluid inlet. And the following.

[0024]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Various embodiments of the present invention will be described below with reference to the drawings.

First, an embodiment of a reagent dispensing apparatus according to the present invention will be described with reference to FIGS. As shown in FIG. 1, a reagent dispensing apparatus according to this embodiment includes a plurality of reagent syringes 10, 1 each filled with a reagent.
., A plurality of reagent syringes 10, 10,..., A plurality of reagent lines 30 for guiding the reagent in the reagent syringe 10 to a target position, and a plurality of reagent syringes for supplying deaerated water (operating fluid). A distributor 51 for distributing every 10, 10,...
The plurality of reagent syringes 10, 10,...
Deaerated water syringe (operating fluid syringe) 44 for supplying deaerated water to a target amount, a plunger 47 for operating the deaerated water syringe 44, and a deaerated water tank 41 for storing the deaerated water.
And the degassed water in the degassed water tank 41
Deaerated water pump 42, a deaerated water supply line 40 connecting the deaerated water tank 41, the deaerated water pump 42, the deaerated water syringe 44 and the distributor 51, a distributor 51 and a plurality of reagent syringes. , A plurality of deaerated water branch lines 50, 50,... Connecting the respective deaerated water in the distributor 51;
Opening / closing solenoid valves 43, 55, provided in 50, 60
63, open / close solenoid valves 43, 55, 63 and deaerated water pump 4
A control device (conversion means) 70 for controlling the second and the like, and a display device (display means) 71 for displaying the dispensing state of the reagent and the like are provided.

Each of the reagent syringes 10 has a syringe casing 11 and a piston 20 that moves inside the syringe casing 11. The syringe casing 11 has a hollow cylindrical shape, a discharge port 13 is formed at one end and an opening 14 is formed at the other end, and an opening 14 of the syringe casing body 12 is formed. And a receiving seat 16 that closes the seat. The discharge line 13 of the syringe casing 11 has a reagent line 3
0 is connected, and a reagent nozzle 31 is provided at the tip thereof. A degassing water inlet 17 is formed in the receiving seat 16. The syringe casing 11 is used with the discharge port 13 facing upward and the degassing water inlet 17 facing downward.
Move up and down inside. As shown in FIG. 2, the piston 20 has a cylindrical shape, and has a piston body 21 having an outer diameter slightly smaller than the inner diameter of the syringe casing body 12, and a fin-shaped member provided on an upper portion of the outer periphery of the piston body 21. It has a seal 24 and a lip-shaped seal 25 provided on the lower part of the outer periphery of the piston body 21. The piston body 21, the fin-shaped seal 24 and the lip-shaped seal 25 are integrally formed to form the piston 20. The fin-shaped seal 24 is formed on the cylindrical piston body 21.
, Extends downward as it moves away from the central axis of the syringe casing 12 and contacts the inner peripheral surface of the syringe casing body 12. The lip-shaped seal 25 protrudes from the outer peripheral surface of the cylindrical piston main body 21 in the outer peripheral direction, and is in contact with the inner peripheral surface of the syringe casing main body 12. The upper end of the piston body 21
The lower end of the piston main body 21 has a substantially conical shape that protrudes downward as it approaches the center axis of the piston main body 21. doing. The piston 20 is the piston 2
The inside of the syringe casing 11 is partitioned into upper and lower two chambers 18 and 19 by the respective seals 24 and 25 of 0. Of the upper and lower two chambers 18 and 19, the upper chamber forms a reagent chamber 19 in which a reagent enters, and the lower chamber forms a degassing water chamber 18 in which degassed water enters.
An iron plate 73 is attached to the lowermost end of the piston 20. Further, a magnetic sensor (detection means) 72 for detecting the position of the iron plate 73 and recognizing the position of the piston 20 is embedded in the receiving seat 16.

As shown in FIG. 1, the degassed water syringe 44 includes a syringe casing 45 and a syringe casing 4.
5, and a piston 46 that moves inside. The plunger 47 is connected to the piston 46.

The dispenser 51 has one deaerated water inlet 52 into which deaerated water from the deaerated water syringe 44 flows, and deaerated water outlets provided by the number of reagent syringes 10, 10,. , And one degassed water discharge port 54 for discharging degassed water are formed. The distributor 51 is provided with each port 5
Out of 2, 53 and 54, the deaerated water discharge port 53 is formed at the lowermost part, the deaerated water discharge port 54 is formed at the uppermost part, and the deaerated water suction port 52 is formed between the discharge port 53 and the discharge port 54. It is formed between them.

The deaerated water supply line 40 is connected to the deaerated water tank 4
1, a deaerated water pump 42, a syringe casing 45 of a deaerated water syringe 44, and a deaerated water inlet 52 of a distributor 51 are connected. An open / close solenoid valve 43 is provided between the deaerated water pump 42 and the deaerated water syringe 44 of the deaerated water supply line 40.

A plurality of deaerated water branch lines 50, 50,...
Are a plurality of deaerated water outlets 53, 53,.
Are connected to the deaerated water inlets 17, 17,... Of the plurality of reagent syringes 10, 10,. Each of the deaerated water branch lines 50, 50,... Is provided with an open / close solenoid valve 55, 55,.

The degassed water discharge line 60 is connected to the degassed water discharge port 54 of the distributor 51 and is divided into two parts on the way, one of which forms a degassed water main discharge line 61 and the other forms a degassed water. A water sub-discharge line 65 is formed. The deaerated water main discharge line 61 is provided with an opening / closing solenoid valve 63 in the middle thereof, and a deaerated water discharge nozzle 62 is provided at the tip thereof. The height of the tip of the degassed water discharge nozzle 62 depends on each of the reagent nozzles 31 and 3.
The height corresponds to the height of the tip of 1,. The degassed water sub-discharge line 65 is provided with a pressure detector (pressure detecting means) 66 and a pressure relief valve 67.

Each of the open / close solenoid valves 43, 55, 63, the deaerated water pump 42, the plunger 47, and the magnetic sensor 72
Each is connected to the control device 70 by a signal line.

The reagent dispensing apparatus of this embodiment is, for example,
It is configured as a part of an automatic analyzer for analyzing samples such as blood and urine. In this case, as shown in FIG. 3, a sample turntable 80 on which various samples are placed and the reagent dispensing apparatus of this embodiment are provided. Here, eight reagent syringes 10, 10,... Are arranged side by side and attached to the slide base 81 to form one reagent syringe group.
Each reagent syringe group is housed in the rack 82 in an orderly arrangement. Each of the reagent nozzles 31 of each of the reagent syringes 10, 10,... Forming one reagent syringe group,
Are fixed to one nozzle plate 83. When it is desired to discharge the reagent in a certain reagent syringe 10 to a target position on the sample turntable 80, the nozzle plate 83 is moved to move the reagent nozzle 31 connected to the reagent syringe 10 onto the sample turntable. Move to the desired position. When a certain reagent syringe group is not used, it is pulled out from the rack 82 together with the slide base 81 to which this reagent syringe group is attached,
This is stored in the refrigerator or the rack 82 is stored in the refrigerator. When the reagent syringe 10 is stored in a refrigerator, the reagent line 30 and the reagent nozzle 31
Is stored while connected to the reagent syringe 10.
When it is desired to replace a certain reagent syringe group, the user pulls out the slide base 81 to which the reagent syringe group is attached from the rack 82, and replaces this reagent syringe group. Thus, the plurality of reagent syringes 10,
.. Are arranged in order and arranged as a unit, so that the installation area of the entire apparatus can be reduced and the handling as a reagent syringe group can be facilitated.

Next, the operation of the reagent dispensing apparatus according to this embodiment will be described. Before dispensing the reagent, the piston 20 of the reagent syringe 10 is
Located at the bottom of the. As described above, the reagent chamber 19 above the piston 20 in the syringe casing 11 is filled with a reagent, and the degassing water chamber 18 below the piston 20 is filled with degassed water. . Further, the piston 46 of the degassed water syringe 44 is also located at the lowermost part in the boiling water syringe casing 45. The on-off solenoid valves 55, 55,... In each of the deaerated water branch lines 50, 50,.
The on-off solenoid valve 63 in the degassed water main discharge line 61 is open. Basically, the degassing water pump 42 is normally driven at all times. For this reason, the degassed water in the degassed water tank 41 is sent into the degassed water syringe casing 45 by the degassed water pump 42, and enter. When the inside of the distributor 51 is filled with degassed water, the degassed water passes through the degassed water main discharge line 61 and is discharged from the degassed water discharge nozzle 62. The dispensing apparatus is in the above state when not actually dispensing.

When actually dispensing, first, the on-off solenoid valve 43 in the degassed water supply line 40 is closed. Then, the plunger 47 is slightly raised to remove the backlash between the piston 46 of the degassed water syringe 44 and the plunger 47. At this time, the degassed water is discharged from the degassed water discharge nozzle 62 by the amount that the piston 46 of the degassed water syringe 44 is slightly raised. At this time, the internal pressure of the head difference from the deaerated water discharge nozzle 62 is applied to the inside of the deaerated water syringe casing 45.

In this embodiment, deaerated water from which the residual air has been removed is used as the working fluid of the piston 20 of the reagent syringe 10. This is because, if air bubbles such as air exist in the working fluid, the air bubbles function as a damper, and the quantitative accuracy of the reagent deteriorates.
However, even if deaerated water is used as the working fluid, the deaerated water is supplied to the deaerated water supply line 40, the deaerated water syringe casing 45, and the distributor 51 in the process of supplying the deaerated water. There is a risk that air will be entrained in the water.
Therefore, in this embodiment, the distributor 51 is positioned above the degassed water syringe 44, and the degassed water discharge line 60 and the degassed water discharge nozzle 62 are positioned above the distributor 51. At the same time, the deaerated water discharge port of the deaerated water syringe 44 is directed upward, and the deaerated water discharge port 54 of the distributor 51 is directed upward. Therefore, even if deaerated water containing air bubbles flows into the deaerated water syringe casing 45,
The air bubbles surely come out of the degassed water discharge port on the upper part of the degassed water syringe casing 45, reach the distributor 51, and come out of the degassed water discharge port 54 on the upper part of the distributor 51 to be degassed. The gas is discharged from the degassed water discharge nozzle 62 through the main steam discharge line 61 without fail. The plurality of deaerated water discharge ports 53 of the distributor 51 are connected to the deaerated water inlet 5 of the distributor 51.
2 and below the deaerated water discharge port 54, even if deaerated water containing air bubbles enters the distributor 51, the air bubbles are generated by the deaerated water discharge ports 53, 53 of the distributor 51. ,
… And the deaerated water branch line 5 connected thereto
0, 50,...

After slightly raising the plunger 47, the opening / closing solenoid valve 63 of the degassed water main discharge line 61 is closed.

Next, a degassed water branch line 50 connected to a reagent syringe 10 filled with a reagent to be discharged.
Is opened. At this time, the on-off solenoid valve 55
, The reagent nozzle 31 side is filled with liquid (reagent and degassed water), and the degassed water discharge nozzle 62 side is also filled with liquid (degassed water). 62 is located at the same level, the head difference between the reagent nozzle 31 side and the degassed water discharge nozzle 62 side is almost zero. Therefore, there is no movement of the liquid in each line, the inside of the reagent syringe casing 11, and the inside of the distributor 51, and the piston 20 of the reagent syringe 10 does not move either. For this reason,
The accuracy and reproducibility of the dispensed amount of the reagent become extremely good.
Then, the plunger 47 is operated to the target amount, and the piston 46 of the degassed water syringe 44 is raised, so that the piston 4
The degassed water corresponding to the volume change in the degassed water syringe casing 45 accompanying the movement of the degassed water 6 is distributed to the distributor 51 and the degassed water branch line 50.
Is sent to the degassing water chamber 18 of the reagent syringe 10, the piston 20 of the reagent syringe 10 is raised, and the degassing water chamber 1 is
The volume in the reagent chamber 19 is reduced while the volume in the reagent chamber 19 is decreased, and the reagent in the reagent chamber 19 is discharged from the reagent nozzle 31. At this time, the discharge amount of the reagent is
Although the feedforward control may be performed with the operation amount of 7, the moving amount of the piston 20 of the reagent syringe 10 is detected by the magnetic sensor 72 in this embodiment, and the detected amount is converted into the reagent discharging amount, which is converted into the reagent discharging amount. The plunger 47 is operated and controlled based on this. The display device 71 displays the amount of the reagent discharged based on the output from the magnetic sensor 72, the remaining amount of the reagent in the reagent syringe 10, or the remaining number of times the reagent can be discharged. When the reagent syringe 10 is brand new, that is, when the reagent syringe 10 has never discharged a reagent, the reagent nozzle 31 is once placed at a position other than the target position, and the plunger 47 is operated. After the reagent is discharged from the reagent nozzle 31 and the reagent line 30 is filled with the reagent, the above operation is performed at the target position of the reagent nozzle 31. After discharging the reagent to a target position in a target amount, the on-off solenoid valve 55 in the deaerated water branch line 50 is closed.

By the way, the piston 2 of the reagent syringe 10
In the process of increasing 0, frictional force between the piston 20 and the inner peripheral surface of the reagent syringe casing 11 becomes a problem. Degassed water and reagents are incompressible fluids, but their compressibility is not completely zero. Also, the compression ratio of the piston 20 is not zero, depending on the material. Therefore, the piston 2
When the frictional force between 0 and the inner peripheral surface of the reagent syringe casing 11 is large, even if degassed water flows into the degassed water chamber 18,
The inflowing deaerated water and the piston 20 are compressed, and the piston 20 rises less than the amount corresponding to the inflow of the deaerated water. As a result, only a smaller amount of reagent than the inflow of degassed water is discharged from the reagent chamber 19.
That is, if the frictional force is large, a difference occurs between the inflow amount of the degassed water and the discharge amount of the reagent, and it becomes impossible to discharge an accurate amount of the reagent.

Therefore, in order to discharge an accurate amount of the reagent, it is necessary to minimize the frictional force between the piston 20 and the inner peripheral surface of the reagent syringe casing 11. Therefore,
Experiments have shown that a condition satisfying the following equation is effective.

5 × Fp <Sp × Po Sp: The step area of the piston, Po: the pressure in the reagent chamber when the reagent is discharged, Fp: the frictional force between the piston and the inner peripheral surface of the reagent syringe casing The frictional force between the piston 20 and the inner peripheral surface of the reagent syringe casing 11 is preferably as small as possible. However, if it is too small, the piston 20 may fall due to its own weight. Therefore, in this embodiment, the piston 20
When the piston 20 is to move upward, the resistance of the liquid is small, and when the piston 20 is to move downward, the resistance of the liquid is large. A fin-shaped seal 24 is provided. Further, in this embodiment, a cavity 26 (shown in FIG. 2) is formed in the piston main body 21 to reduce the weight of the piston 20 and to make the specific gravity of the entire piston 20 close to the specific gravity of the reagent and the degassed water. I have.

When it is desired to discharge another reagent following the above operation, the open / close solenoid valve 55 in the deaerated water branch line 50 to which the reagent syringe 10 filled with the other reagent is connected. Open. Then, the plunger 47 is operated to the target amount, the piston 46 of the degassed water syringe 44 is raised, and the degassed water is supplied to the reagent syringe 1 via the distributor 51.
The reagent is sent to the degassing water chamber 18 of 0, the piston 20 of the reagent syringe 10 is raised, and the reagent in the reagent chamber 19 of the reagent syringe 10 is discharged from the reagent nozzle 31. In addition, at this time, when the reagent nozzles 31 other than the reagent nozzle 31 that discharges the reagent are not used for a relatively long time, in order to prevent the reagent from solidifying in the reagent nozzle 31, pure water is used. It is good to soak. When the amount of degassed water in the degassed water syringe casing 45 becomes smaller than the amount of reagent to be discharged, all the on-off solenoid valves 55 of each degassed water branch line 50 are closed, and Supply line 4
0 and opening and closing solenoid valves 43 and 63 of the degassed water main discharge line 61
And lower the piston 46 of the degassed water syringe 44,
After the degassed water is filled in the degassed water syringe casing 45, the opening / closing solenoid valve 55 in the target degassed water branch line 50 is opened, and the piston 46 of the degassed water syringe 44 is raised, and the target reagent is turned on. The reagent is discharged from the syringe 10.

When the piston 20 of a certain reagent syringe 10 reaches the uppermost point and the reagent in the reagent syringe casing 11 is substantially exhausted, the magnetic sensor 72 detects this, and the display device 71 displays the reagent of the certain reagent syringe 10. Indicates that has expired. In this embodiment, the magnetic sensor 72
Is detected based on the detection of the position of the piston 20 by the above, the pressure of the deaerated water is detected by a pressure detector 66 provided in the deaerated water sub-discharge line 65, and this detection value is determined in advance. It may be configured to recognize that the reagent has run out based on whether or not it is equal to or more than a predetermined value. This is because when the reagent runs out and the piston 20 reaches the highest point, the piston 20 does not move even if the plunger 47 is further operated, and the pressure of the degassed water increases. It is the control device 70 that recognizes that the reagent has run out based on the output from the magnetic sensor 72 and the pressure detector 66. Further, in this embodiment, the position of the piston 20 is detected by the magnetic sensor 72. However, any type can be used as long as the position of the piston 20 can be detected. A plate may be attached and an optical sensor may be provided on the outer peripheral surface of the syringe casing 11 to detect the position of the piston 20.

As described above, in this embodiment, a dispenser system is used for a plurality of reagents, using the nozzles 31, 31,... For the number of reagents and the reagent syringes 10, 10,. Therefore, many disadvantages of the pipetting method such as a long dispensing cycle time can be solved.

Further, in this embodiment, since there is no valve at a place where the reagent passes or exists, an expensive valve having chemical resistance and abrasion resistance to various reagents is not required. Further, since the reagent is not switched back, a three-way valve or a three-way flow path switch is not required. Furthermore, one deaerated water syringe 44 and one plunger 47 are used as driving sources for the plurality of reagent syringes 10, 10,. Therefore, in this embodiment, relatively inexpensive components can be used, and the number of components is small and the overall configuration is relatively simplified, so that manufacturing costs can be reduced. In addition, since there is no valve at the location where the reagent passes or exists, there is no malfunction due to the formation of reagent crystals in the operating portion of the valve, and stable operation can be continuously performed.

In this embodiment, the location where a specific reagent is present or passes is a dedicated reagent line 30 and a dedicated reagent chamber 19 in which degassed water or other reagents as a working fluid cannot exist. In addition, since the locations of the deaerated water and the reagent, which are working fluids, are clearly separated from each other by the piston 20 of the reagent syringe 10, basically no washing water is required. The reagent does not dilute. Therefore, in this embodiment, the consumption of pure water for cleaning can be extremely reduced, and the running cost can be suppressed since the reagent dummy is not required. Further, since there is no need to perform washing while switching from one reagent to another reagent, the dispensing cycle time can be further shortened.

Next, a second embodiment of the reagent syringe according to the present invention will be described with reference to FIG.

The configuration of the reagent syringe 10a of this embodiment other than the piston 20a is the same as that of the first embodiment. The piston 20a has a cylindrical shape and an outer diameter slightly smaller than the inner diameter of the syringe casing body 12, and lip-shaped seals 25a, 25a, 25 protruding from the outer peripheral surface of the piston body 21a.
a. As the lip-shaped seal 25a, an upper lip-shaped seal 25a provided on the outer peripheral surface of the piston body 21a and provided at an upper portion thereof, an intermediate lip-shaped seal 25a provided at an intermediate portion thereof, and a lip-shaped seal 25a provided at a lower portion thereof. There is a lower lip seal 25a provided.
Inner peripheral surface of syringe casing body 12 and piston body 2
1a and respective lip-shaped seals 25a, 25a, 25
A grease is sealed between a and g. This grease not only functions to reduce the frictional force between the inner peripheral surface of the syringe casing main body 12 and the piston 20a, but also serves as a reagent chamber 1
It also has a function of improving the sealing property between the chamber 9 and the degassing water chamber 18.

In this embodiment, the piston 2
When Oa moves upward, a small amount of grease is left at the point where the piston 20a and the inner peripheral surface of the syringe casing body 12 were initially in contact with each other. However, since this reagent syringe is disposable, , Once the piston 20a has been raised, it is lowered and not raised again.
It is not necessary to consider the contamination of the reagent by the grease remaining on the inner peripheral surface of the syringe casing body 12.

Next, a third embodiment of the reagent syringe according to the present invention will be described with reference to FIG.

The configuration of the reagent syringe of this embodiment other than the piston 20b is the same as that of the first embodiment. The piston 20b has a cylindrical shape and has an outer diameter slightly smaller than the inner diameter of the syringe casing main body 12, a lip-shaped seal 25b, 25b, 25b protruding from the outer peripheral surface of the piston main body 21, and the piston 20b. There are a plurality of fins 24b, 24b,... Spreading laterally from the lower part of the main body 21 downward. Like the fin-shaped seal 24 in the first embodiment, the fin 24b reduces the resistance of the liquid when the piston 20b moves upward, and reduces the resistance when the piston 20b moves downward. Plays a role of increasing the resistance of the liquid, but unlike the fin-shaped seal 24 in the first embodiment, has almost no function as a seal. For this reason, the piston 20
In order to enhance the sealing function of b, lip-shaped seals 25b are provided at the upper, middle, and lower portions of the outer peripheral surface of the piston body 21b. In addition, the fin 24b of this embodiment includes:
It is higher in flexibility, that is, softer and longer than the fin-shaped seal 24 in the first embodiment, and the function related to the resistance force of the liquid is further enhanced.

Next, a fourth embodiment of the reagent syringe according to the present invention will be described with reference to FIG.

The reagent syringe of this embodiment has a syringe casing 11c and a piston 20c that moves inside the syringe casing 11c, as in the above embodiments.

The syringe casing 11c has a hollow cylindrical shape, and has a reagent discharge port (not shown) at one end.
The syringe casing body 12c has an opening 14c at the other end, and a receiving seat 16c for closing the opening 14c of the syringe casing body 12c. In the syringe casing main body 12c, a piston main body moving area 15c in which a piston main body 21c described later can move is formed between the reagent discharge port and the opening 14c.
The opening 14c of the syringe casing body 12c is formed to have an inner diameter smaller than the inner diameter of the piston body moving area 15c. In the receiving seat 16c, a degassing water inlet 17c is formed as in the above embodiments,
On the side (inside) that receives the syringe casing body 12c,
Convex portion 16x protruding toward syringe casing body 12c
Are formed.

The piston 20c has a cylindrical shape, and the piston main body movable area 1 of the syringe casing main body 12c.
5c, a piston body 21c having an outer diameter slightly smaller than the inner diameter, a plug portion 27c formed at a lower portion of the piston body 21c, a fin-shaped seal 24c provided at an upper portion of an outer periphery of the piston body 21c, Lip-shaped seal 2 provided on the lower part of the outer periphery of main body 21c
5c. Piston body 21c, stopper 27
c, fin-shaped seal 24c and lip-shaped seal 25c
Are integrally formed to form the piston 20c. The fin-shaped seal 24c extends downward as it goes away from the central axis of the cylindrical piston body 21c. In addition, the lip-shaped seal 25c is attached to the cylindrical piston body 2.
1c protrudes in the outer peripheral direction from the outer peripheral surface. The outer diameters of the fin-shaped seal 24c and the lip-shaped seal 25c are the same as the piston body moving area 15c of the syringe casing body 12.
Slightly larger than the inner diameter of the fin-shaped seal 2
4c and the tip of the lip-shaped seal 25c are pressed against the inner peripheral surface of the piston main body moving area 15c of the syringe casing main body 12c to perform a seal for partitioning the reagent chamber 19c from the degassing water chamber 18c. The outer diameter of the plug 27c is smaller than the inner diameter of the syringe casing main body 12 in the piston main body movement area 15c.
c is slightly larger than the inner diameter of the opening 14c. For this reason,
The stopper 27c is connected to the opening 14 of the syringe casing body 12c.
c, the opening 14c is
7c, while the plug 27c is connected to the piston body moving area 15c of the syringe casing body 12c.
The outer peripheral surface of the plug 27c is in the piston body moving area 15c of the syringe casing body 12.
Does not come into contact with the inner peripheral surface of the piston 20 and does not hinder the movement of the piston 20 at all. The lower portion 28c of the plug 27c has a substantially conical shape that gradually projects downward as it approaches the center axis of the plug 27c.

In this embodiment, except for the receiving seat 16c, the syringe casing body 12c and the reagent chamber 19
It is assumed that the reagent contained in c and the one having the piston 20c are sold or transported as a reagent syringe. At this time, the piston main body 21c is located at the lowermost part of the piston main body moving area 15c, and the opening 14c of the syringe casing main body 12c is connected to the plug 2 of the piston 20c.
7c, the volume of the degassing water chamber 18c is zero, the volume of the reagent chamber 19c is the maximum, and the volume of the reagent chamber 19c is the maximum.
Is filled with reagents.

When the reagent syringe is actually used, as shown in FIG. 6A, deaerated water is filled in a receiving seat 16c previously incorporated as a component of the reagent dispensing apparatus. Then, the syringe casing main body 12c with the opening 14c directed downward is pushed into it. In this process, the plug 27c, which has closed the opening 14c of the syringe casing body 12c, is pushed up by the projection 16x of the receiving seat 16c and rises off the opening 14c. As a result, as shown in FIG. 6B, the entire piston 20c reaches the piston main body moving area 15c, and the piston 20c can move inside the syringe casing main body 12. The plug 27
c has a substantially conical shape that gradually protrudes downward as it approaches the center axis of the plug 27c, so that the syringe casing main body 12c is inserted into the receiving seat 16c in the process of being fitted into the receiving seat 16c. Even if air is trapped between the lower portion 28c and the deaerated water surface in the receiving seat 16c, the air bubbles gradually escape to the outer peripheral side, flow out together with the deaerated water overflowing from the receiving seat 16c, and enter the deaerated water chamber. It is possible to prevent air bubbles from entering the inside 18c.

Next, a fifth embodiment of the reagent syringe according to the present invention will be described with reference to FIGS.

In each of the above embodiments, a piston is used as a partition member for partitioning the inside of the syringe casing into a degassing water chamber and a reagent chamber. In this embodiment, a bag-shaped sheet is used. In this respect, the present embodiment is significantly different from the above embodiments.

As shown in FIG. 7, the reagent syringe 10d of this embodiment has a syringe casing 11d and the above-mentioned sheet 20d. Syringe casing 1
1d has a hollow cylindrical shape and has a discharge port 13d at one end.
Is formed, an opening 14d is formed at the other end, a syringe casing body 12d, a stopper 15d for closing the opening 14d of the syringe casing body 14d, an opening 14d of the syringe casing body 12d and a stopper for closing the opening 14d. And a receiving seat 16d for covering 15d. This reagent syringe 10d is used with the discharge port 13d facing upward, similarly to the reagent syringes of the above embodiments.

The bag-like sheet 20d is made of polyethylene resin having a thickness of 0.3 mm, and the opening of this bag is substantially the same as the size of the opening 14d of the syringe casing body 12d. The opening edge 21d of the bag-shaped sheet 20d is adhered to the inner peripheral surface near the opening 14d of the syringe casing body 12d. The syringe casing 11d is partitioned by the bag-shaped sheet 20d into two chambers: a reagent chamber 19d on the discharge port side and a degassing water chamber 18d on the opening side.

The stopper 15d and the receiving seat 16d are formed with deaerated water inlets 15y and 17d, respectively. The lower portion of the stopper 15d has a substantially conical shape gradually projecting downward along the center axis of the stopper 15d.
With the stopper 15d closing the 2d opening 14d, this conical portion protrudes from the syringe casing body 12d.

In this embodiment, except for the receiving seat 16d, the syringe casing body 12d and the reagent chamber 19d
d, the stopper 15d, and the bag-like sheet 2
It is assumed that the product having 0d is sold or transported as a reagent syringe. At this time, the bag-like sheet 20d
Is a pechanko whose internal volume is almost zero. That is, the capacity of the degassing water chamber 18d is almost zero, and the capacity of the reagent chamber 19 is maximum, and the reagent chamber 19 is filled with the reagent.

When actually using the reagent syringe in the form of sale or transportation, the inside of the degassing water chamber 18d is vacuum-evacuated from the degassing water inlet 17d of the stopper 15d, and then the degassing having an almost zero internal volume is performed. In the water chamber 18d, a deaerated water inlet 1 for the stopper 15d is provided.
Deaerated water is supplied from 7d, and the receiving seat 16d is also filled with deaerated water. Then, the syringe casing body 12d is mounted on the receiving seat 16d to prevent air bubbles from entering the degassing water chamber 18d. When the reagent is discharged from the reagent syringe 10d, the deaerated water chamber 1d is received from the deaerated water inlet 17d of the receiving seat 16d in the same manner as in the above embodiments.
Degassed water flows into 8d. Then, while the volume in the degassing water chamber 18d increases by the amount of inflow of degassed water, the volume in the reagent chamber 19d decreases, so that the reagent in the reagent chamber 19d is discharged from the discharge port 13d. You.

The size of the bag-like sheet is such that when the inner volume thereof is the maximum, that is, when the volume of the degassing water chamber is the maximum, the outer surface of the bag-like sheet (the surface on the reagent chamber side) is the syringe casing body 12d. As shown in FIG. 8, in the case of the size, the outer surface of the bag-shaped sheet 20x partially adheres to the inner peripheral surface of the syringe casing main body 12d in the case of the size of the syringe casing. There is a possibility that an isolated reagent pool 19x may be formed in an intermediate portion in the main body 12d. If the reagent pool 19x is formed, the reagent there is discharged to the outlet 13d.
To the side, and the reagent cannot be discharged smoothly. Therefore, in this embodiment, as shown in FIG. 9, the size of the bag-like sheet 20d is such that when the inner volume is the maximum, the size of the bag-like sheet 20d is between the outer surface of the bag-like sheet 20d and the inner peripheral surface of the syringe casing body 12d. The size is such that a gap d of 0.05 mm can exist. Further, as another method, even if a plurality of grooves extending in the vertical direction are formed in the side wall of the syringe casing main body, the formation of the reagent pool can be prevented. This is because even if a part of the outer surface of the bag-shaped sheet 20d is in close contact with a part of the inner peripheral surface of the syringe casing body, the reagent can move to the discharge port side along the groove. That is, in order to prevent the formation of a reagent pool, when the inner volume of the bag-shaped sheet 20d is the maximum, the outer surface of the bag-shaped sheet 20d and the inner peripheral surface of the syringe casing body 12d should not be in close contact with each other. I just need.

As described above, when the inner volume of the bag-shaped sheet 20d is maximum, if the outer surface of the bag-shaped sheet 20d and the inner peripheral surface of the syringe casing body 12d are not in close contact with each other,
Although the formation of the reagent pool can be prevented, a new problem is that even when the volume of the reagent chamber 19d is minimized, the volume of the reagent chamber 19d is not zero, but is relatively large, and the reagent stored in the reagent chamber 19d is not large. A problem of wasting occurs. Therefore, here, when the inner volume of the bag-shaped sheet 20d is maximized, it comes into contact with the outer surface of the bag-shaped sheet 20d,
A needle (sheet breaking means) 12x for breaking the bag-shaped sheet 20d is provided on the inner peripheral surface of the casing body 12d. When the inner volume of the bag-shaped sheet 20d is maximized and the bag-shaped sheet 20d is broken by the needle 12x, the degassed water in the degassing water chamber 18d flows into the reagent chamber 19d. As a result, the reagent in the reagent chamber 19d is discharged from the reagent chamber 19d, and waste of the reagent can be reduced. As another method for reducing the waste of the reagent, when the pressure in the deaerated water chamber 18d becomes equal to or higher than a predetermined value, a part of the bag-shaped sheet 20d is damaged in advance so that the part is torn. There is also a way to attach it.

As shown in FIG. 7, the reagent dispensing apparatus using the reagent syringe 10d according to this embodiment basically includes a first dispenser.
This is the same as the embodiment. However, in this embodiment, it is practically impossible to accurately detect the amount of deformation of the bag-like sheet 20d constituting the partition member. Therefore, the amount of reagent discharged is determined from the amount of deformation of the bag-like sheet 20d. Can not do. Therefore, in this embodiment, the discharge amount of the reagent is recognized from the operation amount of the plunger 47 for operating the degassed water syringe 44. Further, for the same reason, the amount of the reagent syringe 10d
In this embodiment, a signal from the pressure detector 66 is taken into the control device 70, and the pressure of the degassed water detected by the pressure detector 66 is predetermined. When the value becomes equal to or more than the value, the control device 70 can recognize that the reagent has run out.

[0068]

According to the present invention, since a dispenser system is used for a plurality of reagents using a reagent line corresponding to the number of reagents and a reagent syringe corresponding to the number of reagents, there is contamination between the reagents and a dispensing cycle. Many disadvantages of the pipetting method such as a long time can be solved. Further, in the present invention, since there is no valve at a position where a reagent can be present, an expensive valve having chemical resistance and wear resistance to various reagents is not required. Further, since the reagent is not switched back, a three-way valve or a three-way flow path switch is not required. Further, one working fluid syringe serving as a driving source is provided for a plurality of reagent syringes. Therefore, according to the present invention, relatively inexpensive components can be used, and the number of components is small and the overall configuration is relatively simplified, so that manufacturing costs can be reduced.

Further, in the present invention, since there is no valve at a position where a reagent can be present, there is no malfunction due to the formation of reagent crystals in the operating portion of the valve, and stable operation can be continuously performed.

Further, according to the present invention, the location where a specific reagent exists or passes is a dedicated reagent line and a dedicated reagent chamber in which a working fluid and other reagents cannot exist. Reagents are clearly separated at their existing locations by the partition member of the reagent syringe, so that basically no washing water is required and the water does not dilute the reagent. Therefore, according to the present invention, the consumption of pure water for cleaning can be extremely reduced, and the running cost can be suppressed because the reagent dummy is not required. Further, since there is no need to perform washing while switching from one reagent to another reagent, the dispensing cycle time can be further shortened.

[Brief description of the drawings]

FIG. 1 is a system diagram of a reagent dispensing apparatus as a first embodiment according to the present invention.

FIG. 2 is a sectional view of a main part of a reagent syringe as a first embodiment according to the present invention.

FIG. 3 is a perspective view of an automatic analyzer according to a first embodiment of the present invention.

FIG. 4 is a sectional view of a main part of a reagent syringe as a second embodiment according to the present invention.

FIG. 5 is a sectional view of a main part of a reagent syringe as a third embodiment according to the present invention.

FIG. 6 is a sectional view of a main part of a reagent syringe as a fourth embodiment according to the present invention.

FIG. 7 is a system diagram of a reagent dispensing apparatus as a fifth embodiment according to the present invention.

FIG. 8 is a sectional view of a reagent syringe as a comparative example with respect to the fifth embodiment according to the present invention.

FIG. 9 is a cross-sectional view of a reagent syringe as a fifth embodiment according to the present invention.

[Explanation of symbols]

10, 10d ... reagent syringe, 11, 11c, 11d ...
Syringe casing, 12, 12c, 12d: Syringe casing main body, 12x: Needle, 13, 13d: Reagent discharge port, 14, 14c, 14d: Opening (of the syringe casing main body), 15c: Piston body moving area, 15d ...
Stopper, 16, 16c, 16d ... receiving seat, 17, 17c, 1
7d, 15y ... degassing water inlet, 18, 18c, 18d ...
Degassing water chamber, 19, 19c, 19d ... Reagent chamber, 20, 20
a, 20b, 20c: piston, 20d: bag-like sheet,
21, 21a, 21b, 21d ... piston body, 21d
... Opening edge (of bag-like sheet), 24, 24c ... Fin-shaped seal, 24b ... Fin, 25, 25a, 25b, 25c
... lip-shaped seal, 26, 26a, 26b, 26c ... cavity, 27c ... stopper, 30 ... reagent line, 31 ... reagent nozzle, 40 ... degassed water supply line, 44 ... degassed water syringe,
50: deaerated water branch line, 51: distributor, 52: deaerated water suction port, 53: deaerated water discharge port, 54: deaerated water discharge port, 5
5 ... open / close solenoid valve, 60 ... degassed water discharge line, 61 ... degassed water main discharge line, 62 ... degassed water discharge nozzle, 65 ... degassed water secondary discharge line, 66 ... pressure detector, 67 ... pressure relief Valves 70 Control device 71 Display device 72 Magnetic sensor 80 Sample turntable 81 Slide base 82 Rack 83 Nozzle plate

Claims (14)

[Claims] 1. A reagent dispensing apparatus for dispensing a plurality of reagents to respective target positions, comprising: a plurality of reagent syringes each filled with a plurality of reagents; and a plurality of reagent syringes provided for each of the plurality of reagent syringes. A distributor having a plurality of reagent lines for guiding a reagent in a reagent syringe to a target position, a suction port for sucking a working fluid, and a plurality of discharge ports respectively connected to the plurality of reagent syringes and discharging the working fluid. A working fluid syringe that supplies a target amount of the working fluid to the plurality of reagent syringes via the distributor; and a plurality of connecting the plurality of outlets of the distributor and the plurality of reagent syringes respectively. A working fluid branch line, and a plurality of on-off valves respectively provided in the plurality of working fluid branch lines. A syringe casing in which a medicine outlet and a working fluid inlet are formed, and the interior of the syringe casing is partitioned into two chambers: a reagent chamber on the reagent outlet side and a working fluid chamber on the working fluid inlet side; A partition member that moves in the casing or deforms in the syringe casing to change the volume of the two chambers, the reagent discharge port is connected to the reagent line, and the working fluid inflow port is the working fluid branch. A reagent dispensing device connected to a line. 2. The reagent dispensing device according to claim 1, wherein the partition member is a piston that moves in the reagent syringe casing while an outer peripheral surface of the partition member contacts an inner peripheral surface of the reagent syringe casing. A reagent dispensing apparatus characterized by the above-mentioned. 3. The reagent dispensing apparatus according to claim 2, wherein a cross-sectional area of the piston is Sp, and the piston moves and the reagent is discharged from the reagent discharge port of the reagent syringe casing. When the pressure in the reagent chamber is Po and the frictional force between the outer peripheral surface of the piston and the inner peripheral surface of the syringe casing when the piston is moving is Fp, the relationship of 5 × Fp <Sp × Po is established. A reagent dispensing device characterized by performing: 4. The reagent dispensing device according to claim 2, wherein the piston has a piston main body having an outer diameter smaller than an inner diameter of a reagent syringe casing, the reagent chamber side, and the operation. A seal portion protruding from an outer peripheral surface of the piston main body on the fluid chamber side, an inner peripheral surface of the syringe casing, an outer peripheral surface of the piston main body, the seal portion on the reagent chamber side, and the working fluid chamber side; A grease is filled in a space surrounded by the seal part and the reagent dispenser. 5. The reagent dispensing device according to claim 2, wherein the reagent discharge port of the reagent syringe is located above,
The working fluid inlet is disposed at a lower position, and a moving direction of the piston is a vertical direction. The piston has an outer peripheral surface or a lower portion thereof, which gradually spreads laterally downward. A reagent dispensing device comprising a fin. 6. The reagent dispensing device according to claim 5, wherein a tip portion of the fin contacts an inner peripheral surface of the reagent syringe casing and serves as a seal between the reagent chamber and the working fluid chamber. A reagent dispensing device characterized by having a role. 7. The reagent dispensing device according to claim 2, wherein a detecting means for detecting an amount of movement of the piston of the reagent syringe, and an output from the detecting means, A reagent dispensing apparatus, comprising: a conversion unit for converting into at least one of a discharge amount, a remaining amount of a reagent, or the number of times a reagent can be discharged; and a display unit for displaying the conversion result. 8. The reagent dispensing device according to claim 1, wherein the partition member is a flexible sheet formed in a bag shape, and an opening edge of the bag-shaped sheet is formed of the reagent. A reagent dispensing device adhered to an inner peripheral surface of a syringe casing. 9. The reagent dispensing device according to claim 8, further comprising sheet breakage means for breaking a part of the bag when the bag-shaped sheet is deformed and the volume of the reagent chamber is minimized. A reagent dispensing device, characterized in that: 10. The reagent dispensing device according to claim 1, wherein: a pressure detecting means for detecting a pressure in the working fluid syringe; When the pressure in the working fluid syringe detected by the pressure detecting means is equal to or higher than a predetermined value while the reagent is being discharged from the target reagent syringe of the reagent syringe, the target reagent is Display means for displaying that the reagent in the syringe has run out, and a reagent dispensing apparatus characterized by comprising: 11. The reagent dispensing device according to claim 1, wherein the distributor has a discharge port located above the suction port and the discharge port of the distributor. A working fluid discharge line is connected to the discharge port of the distributor, and an open / close valve is provided in the working fluid discharge line. 12. The reagent dispensing device according to claim 11, wherein an end of the working fluid discharge line is located at the same height as ends of the plurality of reagent lines. Note device. 13. A reagent syringe filled with a reagent, wherein the reagent syringe has a hollow cylindrical shape, and a reagent discharge port is formed at one end.
A syringe casing main body having an opening formed at the other end; and a interior of the syringe casing main body partitioned into two chambers: a reagent chamber on the reagent discharge port side and a working fluid chamber on the opening side. And a reagent that is filled in the reagent chamber. The piston includes a cylindrical piston body that fits in the syringe casing, and a cylindrical piston body that is formed on the outer periphery of the piston body. The syringe casing body has a seal portion that is in close contact with an inner peripheral surface, and a stopper portion formed at an end of the piston body and having an outer diameter smaller than the outer diameter of the seal portion. A piston body moving area in which the piston body can move is formed between the piston body and the opening, and an inner diameter of the opening is smaller than an inner diameter of the piston body moving area. Reagent syringe, characterized in that formed on that may be sized to closed by plugs of the piston. 14. A reagent syringe filled with a reagent, wherein the reagent syringe has a hollow cylindrical shape, and has a reagent discharge port formed at one end thereof.
A syringe casing body having an opening formed at the other end; a flexible and bag-shaped body; an opening edge of the bag is adhered to an inner peripheral surface of the syringe casing body; A sheet partitioning into two chambers: a reagent chamber on the reagent discharge port side and a working fluid chamber on the opening side; a reagent filled in the reagent chamber; and an opening in the syringe casing main body. A reagent syringe, comprising: a formed stopper;