JPH11225750A - Proliferation promoter of bacillus subtilts - Google Patents
Proliferation promoter of bacillus subtiltsInfo
- Publication number
- JPH11225750A JPH11225750A JP3735598A JP3735598A JPH11225750A JP H11225750 A JPH11225750 A JP H11225750A JP 3735598 A JP3735598 A JP 3735598A JP 3735598 A JP3735598 A JP 3735598A JP H11225750 A JPH11225750 A JP H11225750A
- Authority
- JP
- Japan
- Prior art keywords
- bamboo
- bacillus subtilis
- water
- soluble component
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000035755 proliferation Effects 0.000 title abstract description 4
- 241000193830 Bacillus <bacterium> Species 0.000 title 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 44
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 44
- 241001330002 Bambuseae Species 0.000 claims abstract description 44
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 44
- 239000011425 bamboo Substances 0.000 claims abstract description 44
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 34
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 34
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 239000004480 active ingredient Substances 0.000 claims abstract description 3
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 13
- 239000007952 growth promoter Substances 0.000 claims description 8
- 239000000654 additive Substances 0.000 claims description 6
- 230000000996 additive effect Effects 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 2
- 238000012136 culture method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 240000002635 Dendrocalamus asper Species 0.000 abstract description 3
- 235000006555 Dendrocalamus asper Nutrition 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract 1
- 230000002062 proliferating effect Effects 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 108010023063 Bacto-peptone Proteins 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000209128 Bambusa Species 0.000 description 1
- 241000403678 Bambusa blumeana Species 0.000 description 1
- 241001520849 Cephalostachyum pergracile Species 0.000 description 1
- 240000006517 Cinnamomum tamala Species 0.000 description 1
- 235000016386 Cinnamomum tamala Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 241001497770 Melocanna baccifera Species 0.000 description 1
- 235000000434 Melocanna baccifera Nutrition 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000216509 Schizostachyum Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000007218 ym medium Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は枯草菌の増殖促進剤
に関する。TECHNICAL FIELD The present invention relates to a growth promoter for Bacillus subtilis.
【０００２】[0002]
【従来の技術】枯草菌（Bacillus subtilis）はグラム
陽性の好気性細菌であり、土壌中に広く存在し、１）形
質転換が行えること、２）遺伝子地図がよくわかってい
ること、３）胞子形成を行うので細胞分化のモデルとし
て用いられること、４）α−アミラーゼ、プロテアーゼ
等の菌体外酵素を著量分泌すること、５）菌の基本代謝
率がわかっていること、６）遺伝子操作において安全で
あること等の理由から、遺伝子操作の宿主や種々の有用
物質の生産菌等の材料として広く用いられている。BACKGROUND OF THE INVENTION Bacillus subtilis is a gram-positive aerobic bacterium that is widely present in soil, can be transformed, 1) can be transformed, 2) has a good genetic map, and 3) has spores. To be used as a model for cell differentiation because of its formation 4) To secrete a large amount of extracellular enzymes such as α-amylase and protease 5) To know the basic metabolic rate of bacteria, 6) Gene manipulation Because of its safety, it is widely used as a host for genetic engineering and as a material for producing bacteria of various useful substances.
【０００３】かかる枯草菌の増殖因子としては、グルタ
ミン酸やシステインが知られているが、前記の如く枯草
菌の利用範囲が広がるに従って、更に良好な増殖促進剤
が望まれている。[0003] Glutamic acid and cysteine are known as growth factors for Bacillus subtilis. As the use range of Bacillus subtilis expands as described above, a better growth promoter is desired.
【０００４】[0004]
【発明が解決しようとする課題】従来の細菌の増殖促進
剤としては、ビタミン類やアミノ酸類等が多く、かかる
成分はある特定の菌のみを増殖させるのでなく、他の菌
をも増殖させてしまうため、目的とする菌のみを培養し
ようとする場合には適当でない場合が多かった。従っ
て、本発明の目的は、特定の菌、特に枯草菌のみを特異
的に増殖させる作用を有する成分を提供することにあ
る。Conventional bacterial growth promoters include many vitamins and amino acids, and such components not only allow the growth of certain bacteria but also the growth of other bacteria. Therefore, in many cases, it is not appropriate to culture only the target bacterium. Accordingly, an object of the present invention is to provide a component having an action of specifically growing only a specific bacterium, particularly only Bacillus subtilis.
【０００５】[0005]
【課題を解決するための手段】そこで本発明者らは、植
物由来の成分の中から特定の菌に対して増殖又は抑制す
る成分を見出すべく種々検討してきたところ、全く意外
にも竹の水可溶性成分が、枯草菌に対しては増殖促進効
果を有し、他の細菌に対してはほとんど作用しないこと
を見出し、本発明を完成するに至った。Means for Solving the Problems The present inventors have conducted various studies to find a component that can proliferate or inhibit a specific bacterium from plant-derived components. The present inventors have found that a soluble component has a growth promoting effect on Bacillus subtilis and has little effect on other bacteria, thereby completing the present invention.
【０００６】従って、本発明は、竹の水可溶性成分を有
効成分とする枯草菌増殖促進剤を提供するものである。
また、本発明は、竹の水可溶性成分を含有する枯草菌培
養用添加剤を提供するものである。更に、本発明は、竹
の水可溶性成分を添加した培地で枯草菌を培養すること
を特徴とする枯草菌の培養法を提供するものである。Accordingly, the present invention provides a Bacillus subtilis growth promoter comprising a water-soluble component of bamboo as an active ingredient.
The present invention also provides a Bacillus subtilis culture additive containing a water-soluble component of bamboo. Furthermore, the present invention provides a method for cultivating Bacillus subtilis, which comprises culturing Bacillus subtilis in a medium to which a water-soluble component of bamboo is added.
【０００７】[0007]
【発明の実施の形態】本発明の増殖促進剤の有効成分で
ある竹の水可溶性成分としては、竹の圧搾汁、竹の水抽
出物等が挙げられるが、入手容易性の点から竹の圧搾汁
が特に好ましい。また、竹は、繊維が多いことからその
繊維は、広く用いられている。これらの繊維を採取する
場合には、通常、最初に竹を圧搾して水分を除去する操
作が行われるので、竹の圧搾汁はその際の廃物利用の点
でも有利である。なお、竹の水可溶性成分の主な構成成
分を検討したところ糖類及びリグニンであり、これらの
成分の総合作用により枯草菌の増殖が促進されるものと
考えられる。BEST MODE FOR CARRYING OUT THE INVENTION The water-soluble components of bamboo, which are the effective components of the growth promoter of the present invention, include bamboo squeezed juice and bamboo water extract. Pressed juice is particularly preferred. Since bamboo has many fibers, the fibers are widely used. When these fibers are collected, an operation of pressing the bamboo to remove water is usually performed first, so that the pressed juice of the bamboo is also advantageous in terms of utilizing wastes at that time. When the main constituents of the water-soluble component of bamboo were examined, they were saccharides and lignin, and it is considered that the combined action of these components promotes the growth of Bacillus subtilis.
【０００８】また用いられる竹の種類は、特に限定され
ず、リョウリダケ（Dendrocalamusasper）、イガフシタ
ケ（Cephalostachyum pergracile）、ナワタケ（Gigant
ochloa apus）、インドトゲタケ（Bambusa arundinace
a）、マライトゲタケ（Bambusa blumeana）、イチヂク
タケ（Melocanna bambusoides）、タレハタケ（Oxytena
nthera nigrociliata）、タマゴダケ（Schizostachyum
zollingeri）等が挙げられるが、このうちリョウリダケ
が特に好ましい。[0008] The type of bamboo used is not particularly limited, and it is not limited, and the bamboo shoots ( Dendrocalamusasper ), Igafushitake ( Cephalostachyum pergracile ), Nawatake ( Gigant )
ochloa apus , Indian bark ( Bambusa arundinace )
a ), Maraitake mushroom ( Bambusa blumeana ), Ichitake mushroom ( Melocanna bambusoides ), Tarehatake ( Oxytena )
nthera nigrociliata ), Egg ( Schizostachyum )
zollingeri ) and the like, of which Ryoridake is particularly preferred.
【０００９】水可溶性成分を採取する竹の部分として
は、特に限定されず、全体又は竹の幹部分が用いられ
る。なお、水抽出物する場合には、竹を細かく破砕後、
水又は熱水で抽出するのが好ましい。また、竹の圧搾汁
の採取は、竹をローラー等によりプレスすればよい。The portion of the bamboo from which the water-soluble component is collected is not particularly limited, and the whole or the bamboo trunk is used. In the case of water extract, after crushing bamboo finely,
Extraction with water or hot water is preferred. The bamboo squeezed juice may be collected by pressing the bamboo with a roller or the like.
【００１０】竹の水可溶性成分は、後記実施例に示す如
く、酵母類、乳酸菌、黄色ブドウ球菌、大腸菌等に対し
ては増殖促進作用を示さず、枯草菌に対して選択的に増
殖促進作用を示す。従って、枯草菌培養時の添加剤とし
て用いることができる。The water-soluble component of bamboo has no growth-promoting effect on yeasts, lactic acid bacteria, Staphylococcus aureus, Escherichia coli, etc., but has a selective growth-promoting effect on Bacillus subtilis, as shown in the Examples below. Is shown. Therefore, it can be used as an additive at the time of cultivation of Bacillus subtilis.
【００１１】竹の水可溶性成分を添加して枯草菌を培養
すれば、枯草菌の増殖が促進され、その培養目的、例え
ば酵素類の生産、組み込まれた遺伝子産物の生産に極め
て有利である。当該竹の水可溶性成分の培地への添加量
は、特に制限されないが、生竹換算で１〜１０００mg／
mlが好ましい。なお、枯草菌の培養条件は、通常の条件
に従えばよい。When Bacillus subtilis is cultured by adding a water-soluble component of bamboo, the growth of Bacillus subtilis is promoted, which is extremely advantageous for the purpose of culture, for example, production of enzymes and production of incorporated gene products. The amount of the water-soluble component of the bamboo to be added to the medium is not particularly limited, but is 1 to 1000 mg / raw bamboo equivalent.
ml is preferred. In addition, the culture conditions of Bacillus subtilis may be in accordance with ordinary conditions.
【００１２】本発明の枯草菌培養用添加剤には、前記竹
の水可溶性成分以外に、他の増殖因子を配合してもよ
い。The Bacillus subtilis culture additive of the present invention may contain other growth factors in addition to the water-soluble component of bamboo.
【００１３】[0013]
【実施例】次に実施例を挙げて本発明を詳細に説明する
が、本発明はこれに何ら限定されるものではない。Next, the present invention will be described in detail with reference to examples, but the present invention is not limited to these examples.
【００１４】実施例１ タイ産生竹（Dendrocalamus asper）１００kgをローラ
ープレス機（最大プレス圧（線圧）：６００kg／cm）に
より圧搾して圧搾汁を得、遠心及び濾過により固形分を
除去し、竹圧搾汁２．５リットルを得た。Example 1 100 kg of Thai-produced bamboo ( Dendrocalamus asper ) was squeezed by a roller press (maximum press pressure (linear pressure): 600 kg / cm) to obtain a squeezed juice, and the solid content was removed by centrifugation and filtration. 2.5 liters of pressed bamboo juice were obtained.
【００１５】実施例２ シリコンキャップ付き試験管に培地（供試微生物と各培
地及び培養条件は表１）を９ml入れ、オートクレーブ滅
菌（１２０℃、１５分）した。各微生物の種菌を各培地
に植菌後、実施例１で得た竹圧搾汁の水希釈液（１０％
液又は１％液）を各１ml添加してそれぞれ振とう培養し
た（竹圧搾汁の添加濃度はそれぞれ１％、０．１％とな
る）。このとき、コントロールとして竹圧搾汁未添加の
培地にも植菌し、同様に振とう培養した。３日間培養
後、培養液の濁度を測定し、コントロールの濁度と比較
することによって、竹圧搾汁の各微生物の生育に対する
影響を評価した。Example 2 A test tube with a silicon cap was charged with 9 ml of a medium (test microorganisms, each medium and culturing conditions in Table 1), and sterilized in an autoclave (120 ° C., 15 minutes). After inoculating each medium with the inoculum of each microorganism, the water dilution of the bamboo squeezed juice obtained in Example 1 (10%
(1% solution or 1% solution) was added to each and cultured with shaking (the added concentrations of bamboo press juice were 1% and 0.1%, respectively). At this time, as a control, the cells were inoculated into a culture medium without bamboo squeezed juice and cultured similarly with shaking. After cultivation for 3 days, the turbidity of the culture was measured and compared with the turbidity of the control to evaluate the effect of bamboo squeezed juice on the growth of each microorganism.
【００１６】[0016]
【表１】 [Table 1]
【００１７】＜培地組成＞ （１）ＹＭ培地 グルコース １０．０ｇ バクト−ペプトン ５．０ｇ バクト−イーストエキス ３．０ｇ モルトエキス ３．０ｇ 蒸留水 １．０Ｌ pH ６．２<Medium composition> (1) YM medium Glucose 10.0 g Bacto-peptone 5.0 g Bacto-yeast extract 3.0 g Malt extract 3.0 g Distilled water 1.0 L pH 6.2
【００１８】（２）ＧＡＭ培地 ＧＡＭブロス（Nissui） ５９．０ｇ 蒸留水 １．０Ｌ(2) GAM medium GAM broth (Nissui) 59.0 g distilled water 1.0 L
【００１９】 （３）ニュートリエントブロス＋０．５％NaCl培地 バクト−ペプトン ５．０ｇ 肉エキス ３．０ｇ NaCl ５．０ｇ 蒸留水 １．０Ｌ pH ７．０(3) Nutrient broth + 0.5% NaCl medium Bacto-peptone 5.0 g Meat extract 3.0 g NaCl 5.0 g Distilled water 1.0 L pH 7.0
【００２０】（４）ニュートリエントブロス培地 肉エキス ３．０ｇ バクト−ペプトン ５．０ｇ 蒸留水 １．０Ｌ pH ６．８(4) Nutrient broth medium Meat extract 3.0 g Bacto-peptone 5.0 g Distilled water 1.0 L pH 6.8
【００２１】（５）Ｌ培地 バクト−トリプトン １０．０ｇ バクト−イーストエキス ５．０ｇ NaCl ５．０ｇ グルコース １．０ｇ 蒸留水 １．０Ｌ pH ７．２(5) L medium Bacto-tryptone 10.0 g Bacto-yeast extract 5.0 g NaCl 5.0 g Glucose 1.0 g Distilled water 1.0 L pH 7.2
【００２２】（５）Ｋ２８培地 １／２人工海水（Jamarin S) １．０Ｌ バクト−ペプトン ５．０ｇ バクト−イーストエキス １．０ｇ pH ７．２〜７．４(5) K28 medium 1/2 artificial seawater (Jamarin S) 1.0 L Bacto-peptone 5.0 g Bacto-yeast extract 1.0 g pH 7.2-7.4
【００２３】得られた結果を表２に示す。The results obtained are shown in Table 2.
【００２４】[0024]
【表２】 [Table 2]
【００２５】表２より、本発明の竹の水可溶性成分を培
地へ添加して培養すると、他の菌類に対してはその生育
に特に影響を与えなかったが、枯草菌に対しては強い増
殖促進効果が認められた。As shown in Table 2, when the water-soluble component of the bamboo of the present invention was added to the medium and cultured, it did not particularly affect the growth of other fungi, but showed strong growth against Bacillus subtilis. A promoting effect was observed.
【００２６】[0026]
【発明の効果】本発明の増殖促進剤を添加して枯草菌を
培養すれば、枯草菌の増殖が特異的に促進される。従っ
て、枯草菌の培養効率、枯草菌が産生する各種物質の生
産性向上に有用である。また枯草菌を用いた活性汚泥へ
の添加剤としても有用である。When the Bacillus subtilis is cultured by adding the growth promoter of the present invention, the proliferation of the Bacillus subtilis is specifically promoted. Therefore, it is useful for improving the culture efficiency of Bacillus subtilis and the productivity of various substances produced by Bacillus subtilis. It is also useful as an additive to activated sludge using Bacillus subtilis.
Claims (6)
菌増殖促進剤。A Bacillus subtilis growth promoter comprising a water-soluble component of bamboo as an active ingredient.
請求項１記載の枯草菌増殖促進剤。2. The Bacillus subtilis growth promoter according to claim 1, wherein the water-soluble component of bamboo is a squeezed juice of bamboo.
用添加剤。3. An additive for culturing Bacillus subtilis, comprising a water-soluble component of bamboo.
請求項３記載の枯草菌培養用添加剤。4. The additive for cultivating Bacillus subtilis according to claim 3, wherein the water-soluble component of bamboo is a pressed juice of bamboo.
菌を培養することを特徴とする枯草菌の培養法。5. A method for cultivating Bacillus subtilis, comprising culturing Bacillus subtilis in a medium containing a water-soluble component of bamboo.
請求項５記載の培養法。6. The culture method according to claim 5, wherein the water-soluble component of bamboo is a pressed juice of bamboo.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3735598A JPH11225750A (en) | 1998-02-19 | 1998-02-19 | Proliferation promoter of bacillus subtilts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3735598A JPH11225750A (en) | 1998-02-19 | 1998-02-19 | Proliferation promoter of bacillus subtilts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11225750A true JPH11225750A (en) | 1999-08-24 |
Family
ID=12495253
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3735598A Pending JPH11225750A (en) | 1998-02-19 | 1998-02-19 | Proliferation promoter of bacillus subtilts |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH11225750A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007215484A (en) * | 2006-02-16 | 2007-08-30 | Yamaguchi Prefecture | Method for producing bamboo shoot fermented food and bamboo shoot fermented food |
| JP2008094733A (en) * | 2006-10-06 | 2008-04-24 | Micro Media Japan Corp | Method for producing bamboo liquid and bamboo liquid |
| JP2016044139A (en) * | 2014-08-22 | 2016-04-04 | 学校法人東京電機大学 | Bamboo extract and novel glycoside |
| CN105567769A (en) * | 2016-01-26 | 2016-05-11 | 湖北工业大学 | Synergist for bacillus subtilis capable of generating antibacterial peptides through fermentation and use method of synergist |
-
1998
- 1998-02-19 JP JP3735598A patent/JPH11225750A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007215484A (en) * | 2006-02-16 | 2007-08-30 | Yamaguchi Prefecture | Method for producing bamboo shoot fermented food and bamboo shoot fermented food |
| JP2008094733A (en) * | 2006-10-06 | 2008-04-24 | Micro Media Japan Corp | Method for producing bamboo liquid and bamboo liquid |
| JP2016044139A (en) * | 2014-08-22 | 2016-04-04 | 学校法人東京電機大学 | Bamboo extract and novel glycoside |
| CN105567769A (en) * | 2016-01-26 | 2016-05-11 | 湖北工业大学 | Synergist for bacillus subtilis capable of generating antibacterial peptides through fermentation and use method of synergist |
| CN105567769B (en) * | 2016-01-26 | 2019-02-05 | 湖北工业大学 | A kind of synergist for Bacillus subtilis capable of fermenting antimicrobial peptides and using method thereof |
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