JPH11160310A - Blood corpuscle calculating board - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a blood corpuscle calculating board capable of easily and accurately maintaining a minute gap between the upper surface of a calculating board main body on which a counting scale is formed and the inner surface of an observation plate and to provide a blood corpuscle calculating board in which a counting scale can be easily provided on the upper surface of a calculating board main body. SOLUTION: A blood corpuscle calculating board is provided with a calculating board main body 50 with a counting scale on the flat upper surface and a transparent observation plate 60 which covers the counting scale and is fixed to the upper surface with an adhesive 70. A spacer 71 to maintain the upper surface with the counting scale and the inner surface of the observation plate 60 in parallel with each other at a minute constant interval is mixed into the adhesive 70.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a hemocytometer used for counting the number of blood cells in blood. More specifically, the present invention relates to a hemocytometer suitable as a disposable type discarded after one use.

[0002]

2. Description of the Related Art As shown in FIG. 1, a conventional hemocytometer comprises a calculator main body 10 and a glass plate 20. As shown in FIG. The calculation plate main body 10 is a rectangular flat plate-like member, and has four lands 11 by providing predetermined grooves.
~ 14 are formed. Two lands 13 in the center
And 14 are provided on the upper surface with lattice-shaped 'scribed parts' 15,16. These 'marking portions' serve as counting scales for facilitating the counting of blood cells, as described later. On the other hand, the glass plate 20 is placed and fixed on the upper surface side of the calculation plate main body 10, as shown by the arrow in FIG. The fixation is performed by wetting the periphery of the glass plate with water and utilizing the surface tension.

FIG. 2 is an enlarged view of a portion indicated by a dashed circle A in FIG. 1 with a glass plate 20 mounted and fixed. Lands 11 and 14 are 0.1m high
A difference of about m (100 μ) is provided. As a result, a space 30 having a constant width is formed between the land upper surface and the glass plate 20 (the same applies to the land 13). When the diluted blood is injected into the space 30 with a syringe or the like, the diluted blood fills the space 30 due to surface tension, and thus enters the flat space having the 'scribed portions' 15 and 16 as bottom surfaces. Will always have a fixed volume of diluted blood.

FIG. 3 shows an example of a “marking portion” as a counting scale formed on the upper surfaces of the lands 13 and 14. With the diluted blood filled in the space between the calculation plate body 10 and the glass plate 20 as described above, the 'scribed parts' 15, 16 are passed through a transparent glass plate (observation plate) 20 with a microscope or the like. Observe. Then, the number of blood cells present in each cell of the 'scribed part' is counted sequentially, and when summed up at last, it is present in a flat space having the 'scribed part' 15 and 16 as a bottom surface. The number of blood cells in the diluted blood can be known. Since the volume of the diluted blood in this portion is known, the blood cell concentration can be determined by calculating the number of blood cells per unit volume and multiplying by the magnification at the time of dilution.

[0005] By the way, the above-mentioned main body of the hemocytometer is conventionally made of glass, and is washed and used repeatedly after use. Then, on the glass calculation board main body, "Between adjacent lands as described above
Polishing work to provide a minute height difference of about 0.1 mm is required '', and `` 'marking work' for providing 'marking part' (counting scale) with minute squares is required That is very expensive.

[0006]

Accordingly, the technical problem to be solved by the present invention is to simplify the minute spacing between the upper surface of the main body of the calculation plate on which the counting scale is formed and the inner surface of the observation plate. It is an object of the present invention to provide a hemocytometer that can be maintained accurately, and to provide a hemocytometer that can easily provide a counting scale on the upper surface of the main body of the calculator.

[0007]

SUMMARY OF THE INVENTION The present invention has been made in order to effectively solve the above-mentioned problems, and an object thereof is to provide a hemocytometer having the following configuration.

That is, the blood cell counting plate of the present invention comprises a counting plate main body having a blood cell counting scale (lattice-like 'scribed portion') on a flat upper surface, and a blood cell counting scale covering the upper surface. A transparent observation plate fixed with an adhesive, and the adhesive, the adhesive, the upper surface provided with a blood cell counting scale and the inner surface of the observation plate parallel and A spacer for maintaining a minute constant interval is mixed.

[0009] The hemocytometer of the above construction is suitable for use as a disposable type which is discarded only once and used because the main body and the observation plate are fixed with an adhesive. Yes (of course, not limited to disposable types). Then, the gap between the upper surface of the calculation plate main body and the inner surface of the observation plate can be kept small and constant without performing the polishing operation for providing a slight height difference between the lands as described in FIG. As a result, manufacturing costs can be reduced, and therefore, a hemocytometer at an economical price can be provided.

It is preferable that the spacer is a spherical particle having a constant particle diameter (eg, glass, metal, or resin beads). Since the spherical particles make point contact with the upper surface of the calculation plate body provided with the blood cell counting scale and the inner surface of the observation plate, the distance between both surfaces is kept constant without being affected by the presence of the adhesive. can do.

The blood cell counting plate of the present invention is preferably an injection-molded article in which at least the calculation plate body is made of a plastic resin. In that case, the counting scale on the calculation board body is also integrally formed by injection molding, and therefore,
'Scribing work' can also be omitted, and the manufacturing cost can be further reduced.

[0012]

Embodiments of the present invention will be described below in detail with reference to the accompanying drawings. FIG. 4 is a perspective view showing an embodiment of the blood cell count plate of the present invention, and corresponds to FIG. FIG. 5 is an enlarged view of a portion indicated by a dashed circle B in FIG. 4 in a state where a transparent plastic plate (observation plate) 60 is placed and fixed.

In the present invention, there is no height difference between the adjacent lands 51 and 54, 53 of the calculation panel main body 50, and the top surfaces of all the lands 51 to 54 are flat one plane. Exists within. The small constant width to be secured between the inner surface of the transparent observation plate and the upper surface of the land is mixed in the adhesive 70 used to adhere the observation plate 60 to the calculation panel body 50. This is achieved by making the beads 71 having a constant particle size function as spacers.

The observation plate 60 is made of glass (or may be made of plastic resin), and is fixed to the main body 50 by an adhesive 70 applied to a peripheral portion thereof. The calculation board body 50 is made of plastic resin, and the land 5
On the upper surfaces of 3, 54, grid-like counting scales 55, 56 similar to those shown in FIG. 3 are formed. Counting scale 55, 56
Is integrally formed at the time of injection molding, so that a 'marking operation' such as a case where a counting scale ('marking portion') is formed on a glass calculation plate main body is unnecessary. The specific usage of the hemocytometer of the present invention is the same as that of the conventional hemocytometer described above.

The main body 50 is manufactured by injection molding using a mold. Therefore, it is necessary to form a fine lattice pattern on the cavity inner surface of the mold corresponding to the counting scales 55 and 56, which means that the plate material constituting the mold is subjected to electric discharge machining, printing or etching. Done by Instead of such a forming method, a grid-like counting scale can be directly 'marked' on the land surface.

The space between the observation plate 60 and the land 54 is filled with diluted blood using a syringe or the like, but the recess 59 between the lands 51 and 54 causes excess blood to overflow. Therefore, when the blood cells are counted for different sample blood using the lands 53 and 54, the sample blood is not mixed. Therefore,
In some cases, the entire upper surface of the calculation plate body may be formed of a single flat surface without providing the recess 59.

In order to secure a certain width between the upper surface of the land on which the counting graduations 55 and 56 are formed and the inner surface of the observation plate 60, beads 71 having a particle size of about 0.1 mm are provided on the adhesive 70. Many are mixed (see FIG. 5, in which only one bead appears).

In general, since the particle size of the beads is very uniform, the width between the land surface and the observation plate inner surface can be ensured accurately. However, for this purpose, the beads need to have a certain degree of hardness. Therefore, it is preferable that the beads are made of glass, metal, resin, or the like. In addition, since the beads are spherical, they come into point contact with the surface on the land and the inner surface of the observation plate, and the distance between both surfaces is kept constant without being substantially affected by the presence of the adhesive. be able to.

It is also conceivable to adopt a configuration in which a cylindrical fiber material is adopted as the spacer instead of the spherical beads, and the circumferential surface thereof is brought into linear contact with the land upper surface and the observation plate inner surface. Also in this case, the distance between both surfaces can be kept constant without being substantially affected by the presence of the adhesive.

As described above, the conventional hemocytometer is used with the observation plate detachably attached to the main body of the glass calculation plate, and is washed and used repeatedly after use. On the other hand, the hemocytometer of the present invention is suitable as a disposable type in which the observation plate is fixed to the main body of the calculation plate with an adhesive, and is discarded only once.

In the present invention, the manufacturing cost is suppressed by eliminating the need to provide a small height difference between adjacent lands. In this regard, even when the counter plate body is made of glass, a plastic injection molded product is used. The same is true even if However, when the counting plate body is a plastic injection molded product, the “marking operation” for forming the counting scale can be omitted, so that the manufacturing cost can be further reduced.

[Brief description of the drawings]

FIG. 1 is a perspective view showing a conventional hemocytometer.

FIG. 2 is an enlarged view showing a portion indicated by a dashed circle A in FIG. 1 in an enlarged manner.

FIG. 3 is a scribe section formed on the hemocytometer of FIG. 1;
FIG.

FIG. 4 is a perspective view showing a hemocytometer of the present invention.

FIG. 5 is an enlarged view showing a portion indicated by a dashed circle B in FIG. 4 in an enlarged manner.

[Explanation of symbols]

 10 Calculation board body 11, 12, 13, 14 Land 15, 16 Scribing part 20 Observation plate 30 Space of fixed width 50 Calculation board body 51, 52, 53, 54 Land 55, 56 Count scale 59 Recess 60 Observation plate 70 Adhesion Agent 71 Bead 80 Constant width space

Continuing from the front page (72) Inventor Taiji Kawasaki 14-5 Shimogita-cho, Neyagawa-shi, Osaka Kurashiki Textile Co., Ltd. (72) Inventor Tomoyuki Yokoyama 447 Imakawa Kawarumachi, Kawaramachi-dori, Kamigyo-ku, Kyoto, Kyoto, Japan −14 Yamato Corporation

Claims (3)

[Claims] 1. A blood cell counting scale (55, 56) on a flat upper surface.
And a transparent observation plate (60) that covers the blood cell counting scale and is fixed to the upper surface with an adhesive (70). The adhesive is characterized in that a spacer (71) for keeping the upper surface provided with the blood cell counting scale and the inner surface of the observation plate parallel and at a minute constant spacing is mixed,
Hemocytometer. 2. The spacer comprises a spherical particle (7
The hemocytometer according to claim 1, wherein 1) is satisfied. 3. The hemocytometer according to claim 1, wherein at least the main body of the calculator is an injection-molded article made of a plastic resin.