JPH11160297A - Solid phase extracting apparatus - Google Patents
Solid phase extracting apparatusInfo
- Publication number
- JPH11160297A JPH11160297A JP9330312A JP33031297A JPH11160297A JP H11160297 A JPH11160297 A JP H11160297A JP 9330312 A JP9330312 A JP 9330312A JP 33031297 A JP33031297 A JP 33031297A JP H11160297 A JPH11160297 A JP H11160297A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- filler
- sample
- sample liquid
- phase extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6047—Construction of the column with supporting means; Holders
Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、分析成分の抽出を
固相の充填剤を用いて行う固相抽出装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a solid phase extraction apparatus for extracting an analytical component using a solid phase filler.
【０００２】[0002]
【従来の技術】多くの物質が混在するサンプルから微量
の物質を分析する方法として、ＨＰＬＣ（高速液体クロ
マトグラフィー），ＧＣ（ガスクロマトグラフィー），
分光分析等の機器分析が知られている。このような機器
分析では、通常サンプルに対して溶液状態への調整や、
分析上で妨害となる物質がない状態への精製や、又、測
定や検出に適した濃度への調整等の前処理を行ってい
る。この前処理の一方法として固相抽出法が知られてい
る。2. Description of the Related Art HPLC (High Performance Liquid Chromatography), GC (Gas Chromatography),
Instrumental analysis such as spectroscopic analysis is known. In such instrumental analysis, usually adjustment of the sample to a solution state,
Pretreatment such as purification to a state where there is no substance that interferes with analysis and adjustment to a concentration suitable for measurement and detection are performed. As one method of this pretreatment, a solid phase extraction method is known.
【０００３】この固相抽出法は、分析成分を含む試料液
を充填剤に送液し、充填剤に分析成分を保持させた後、
有機溶剤等によって充填剤から分析成分を溶出させるも
のであり、簡便な操作で目的とする分析成分の抽出を行
うことができるという特徴を備えている。In this solid phase extraction method, a sample liquid containing an analytical component is sent to a filler, and the filler retains the analytical component.
It elutes an analytical component from a filler by an organic solvent or the like, and has a feature that a target analytical component can be extracted by a simple operation.
【０００４】従来、固相抽出法を実施するには、充填剤
を収納したカートリッジと、このカートリッジ内に試料
液を送液するためのシリンジや注射器を用い、この構成
によって、溶液状のサンプルをシリンジ内に採り、この
シリンジからカートリッジ等に収納された充填剤にサン
プルを相液することによって分析成分を充填剤に保持さ
せ、これによって分析成分の抽出を行っている。又、充
填剤としては、Ｃ18，シリカ，フロリシル，不活性けい
藻土，アルミナ等の各種官能基が用いられる。Conventionally, in order to carry out the solid phase extraction method, a cartridge containing a filler and a syringe or a syringe for sending a sample liquid into the cartridge are used. The sample is taken in a syringe, and the sample is mixed with the filler contained in a cartridge or the like from the syringe to hold the analytical component in the filler, thereby extracting the analytical component. Various functional groups such as C 18 , silica, florisil, inert diatomaceous earth, and alumina are used as the filler.
【０００５】[0005]
【発明が解決しようとする課題】従来、固相抽出法によ
って分析成分の抽出を行う場合、微粒子状の物質が含ま
れる試料液やＳＳ分（水中の固形物分）が含まれる試料
液については、分析成分の抽出が困難であるという問題
点がある。固相抽出法では、固相の充填剤を用いている
ため、試料液中の微粒子がカートリッジ内に詰まって試
料液をカートリッジ内に送液を行うことができなくな
り、分析成分の抽出が困難となる。Conventionally, when an analytical component is extracted by a solid phase extraction method, a sample liquid containing fine-particle substances or a sample liquid containing SS (solid matter in water) is not used. However, there is a problem that it is difficult to extract an analysis component. In the solid phase extraction method, since a solid phase filler is used, the fine particles in the sample liquid are clogged in the cartridge, and the sample liquid cannot be sent into the cartridge. Become.
【０００６】これを解決するために、高圧で送液を行う
ことも考えられるが、カートリッジが微粒子で詰まった
状態において高い圧力で送液を行うと、シリンジ等の容
器の外壁が変形して試料液が漏洩することになり、分析
成分の抽出を行うことができない。図６は、従来の固相
抽出の構成で高圧送液を行う場合を説明するための断面
図である。図６において、シリンジ２０’の一端に充填
剤１１’を収納したカートリッジ１０’を設け、押圧部
２１’でシリンジ２０’内のを押圧すると、カートリッ
ジ１０’内が微粒子やＳＳ分で詰まった状態ではシリン
ジ２０’内の圧力が高まり、シリンジ２０’の外壁部分
が変形し、押圧部２１’との間に隙間（図６中のＡで示
す部分）が生じ、この部分からシリンジ２０’内の試料
液が外部に漏れることになる。In order to solve this problem, it is conceivable to send the solution at a high pressure. However, if the solution is sent at a high pressure in a state where the cartridge is packed with fine particles, the outer wall of a container such as a syringe is deformed and the sample is deformed. The solution leaks, and the analysis component cannot be extracted. FIG. 6 is a cross-sectional view for explaining a case where high-pressure liquid sending is performed in a conventional solid-phase extraction configuration. In FIG. 6, a cartridge 10 'containing a filler 11' is provided at one end of a syringe 20 ', and when the inside of the syringe 20' is pressed by a pressing portion 21 ', the cartridge 10' is clogged with fine particles or SS. In this case, the pressure inside the syringe 20 'is increased, the outer wall portion of the syringe 20' is deformed, and a gap (a portion indicated by A in FIG. 6) is generated between the syringe 20 'and the pressing portion 21'. The sample liquid will leak to the outside.
【０００７】又、固相抽出法は微量のサンプルからの分
析成分を抽出することがするという点を特徴の一つとす
る抽出法であるため、大量のサンプルから分析成分を抽
出することを前提とせず、一般には、１ｍｌ，３ｍｌ，
６ｍｌ，１２ｍｌ，２０ｍｌ程度の容量のシリンジを用
いている。そのため、多量のサンプルから分析成分を抽
出する場合には、シリンジ内への試料のサンプリングと
カートリッジへの送液を繰り返したり、多量の処理液を
高圧で送液する必要があり、多量のサンプルからの分析
成分の抽出に適さないという問題点もある。[0007] Further, since the solid-phase extraction method is one of the features of extracting an analytical component from a very small amount of sample, it is assumed that the analytical component is extracted from a large amount of sample. In general, 1ml, 3ml,
A syringe having a capacity of about 6 ml, 12 ml, and 20 ml is used. Therefore, when extracting an analytical component from a large amount of sample, it is necessary to repeatedly sample the sample into the syringe and send it to the cartridge, or to send a large amount of processing solution at high pressure. There is also a problem that it is not suitable for the extraction of the analysis component.
【０００８】さらに、微粒子状の物質が含まれる試料液
やＳＳ分を含む試料液を多量に用いて分析成分の抽出を
行う場合は、より抽出は困難となる。Further, when a large amount of a sample solution containing fine particles or a sample solution containing SS is used to extract an analytical component, the extraction becomes more difficult.
【０００９】そこで、本発明は前記した従来の問題点を
解決し、微粒子状の物質が含まれる試料液やＳＳ分を含
む試料液の固相抽出を行うことができる固相抽出装置を
提供することを目的とし、又、多量の試料液の固相抽出
処理を行うことができる固相抽出装置を提供することを
目的とする。Accordingly, the present invention solves the above-mentioned conventional problems and provides a solid-phase extraction apparatus capable of performing solid-phase extraction of a sample solution containing particulate matter or a sample solution containing SS. It is another object of the present invention to provide a solid-phase extraction device capable of performing a solid-phase extraction process on a large amount of a sample solution.
【００１０】[0010]
【課題を解決するための手段】本発明の固相抽出装置
は、固相抽出用の充填剤に試料液を送液する送液手段
と、前記送液手段内の試料液を加圧する加圧手段と、送
液手段を保持する保持手段を備えた構成とし、保持手段
は送液手段の加圧による送液手段の変形を制限する機能
を備えたものとする。この構成によって、微粒子状の物
質が含まれる試料液やＳＳ分を含む試料液や、多量の試
料液についても固相抽出を行うことができる。According to the present invention, there is provided a solid phase extraction apparatus comprising: a liquid sending means for sending a sample liquid to a filler for solid phase extraction; And a holding means for holding the liquid sending means, and the holding means has a function of restricting deformation of the liquid sending means due to pressurization of the liquid sending means. With this configuration, solid-phase extraction can be performed on a sample liquid containing fine-particle substances, a sample liquid containing SS, or a large amount of sample liquid.
【００１１】本発明の送液手段は、試料液や溶剤を充填
剤内に送り込む手段であり、一実施形態では、一端に充
填剤側と連通する開口部を備えた筒状体と、この筒状体
内をスライドする押圧部とを備えた注射器状の機構で構
成することができる。この注射器状の機構の容量を大き
くすることによって、一度に多量の試料液を充填剤に送
液することができる。The liquid feeding means of the present invention is a means for feeding a sample liquid or a solvent into the filler, and in one embodiment, a cylindrical body having an opening at one end communicating with the filler, It can be constituted by a syringe-like mechanism provided with a pressing portion that slides in the body. By increasing the capacity of the syringe-like mechanism, a large amount of sample liquid can be sent to the filler at one time.
【００１２】本発明の加圧部は、送液手段内の試料液を
加圧して試料液を充填剤側に送り込む手段であり、送液
手段の押圧部を押すための、手動、電動、油圧、空圧等
の任意の機構を適用することができる。この加圧部で送
液手段内の試料液の圧力を高めることによって、微粒子
状の物質やＳＳ分でカートリッジ内が詰まった状態であ
っても、又、多量の試料液であっても、送液を行うこと
ができる。The pressurizing section of the present invention is a means for pressurizing the sample liquid in the liquid sending means and sending the sample liquid to the filler side. Any mechanism such as air pressure or the like can be applied. By increasing the pressure of the sample liquid in the liquid sending means by the pressurizing unit, even if the cartridge is clogged with particulate matter or SS, or even if a large amount of sample liquid is supplied, Liquid can be done.
【００１３】本発明の保持手段は、送液手段を保持する
手段である。送液手段の加圧による送液手段の変形を制
限する機能の一実施形態は、送液手段の外周部分を外側
から被う筒状体を備え、これによって、加圧による送液
手段の外周部分の膨張を抑え、送液手段の変形によって
生じる液漏れを防止することができる。[0013] The holding means of the present invention is means for holding the liquid sending means. One embodiment of the function of limiting the deformation of the liquid sending means due to the pressurization of the liquid sending means includes a cylindrical body which covers the outer peripheral portion of the liquid sending means from the outside, whereby the outer periphery of the liquid sending means by pressurization The expansion of the portion can be suppressed, and the liquid leakage caused by the deformation of the liquid sending means can be prevented.
【００１４】本発明の固相抽出装置によれば、送液手段
内に試料液等の溶液を注入し、その一端に充填剤を収納
したカートリッジを取り付ける。加圧手段によって、送
液手段内の試料液の圧力を高めると、試料液は充填剤内
に送液される。試料液中に微粒子状の物質やＳＳ分が含
まれる場合であっても、又、試料液が多量の場合であっ
ても、この加圧によって送液を行うことができる。この
とき、送液手段は保持手段によって保持されるため、加
圧による変形は抑えられて、液漏れ等を起こすことはな
い。According to the solid phase extraction device of the present invention, a solution such as a sample solution is injected into the liquid sending means, and a cartridge containing a filler is attached to one end of the solution. When the pressure of the sample liquid in the liquid sending means is increased by the pressurizing means, the sample liquid is sent into the filler. Even when the sample liquid contains particulate matter or SS, or when the amount of the sample liquid is large, the liquid can be sent by this pressurization. At this time, since the liquid sending means is held by the holding means, deformation due to pressurization is suppressed, and no liquid leakage or the like occurs.
【００１５】したがって、本発明の構成によれば、微粒
子状の物質が含まれる試料液やＳＳ分を含む試料液や多
量の試料液について、固相抽出を行うことができる。Therefore, according to the configuration of the present invention, solid phase extraction can be performed on a sample liquid containing fine particles, a sample liquid containing SS, or a large amount of sample liquid.
【００１６】[0016]
【発明の実施の形態】以下、本発明の実施の形態を図を
参照しながら詳細に説明する。図１は本発明の固相抽出
装置の第１の構成例を説明するための斜視図であり、一
部分を切り欠いて示している。又、図２は本発明の固相
抽出装置の第１の構成例を説明するための断面図であ
る。第１の構成例は、送液手段２０（２１，２２）と、
充填剤１１を保持するカートリッジ１０とを連続させ、
加圧手段（３０，３１，３２）によって送液手段（２
１，２２）及びカートリッジ１０の両端に圧力を印加す
る構造とするものである。Embodiments of the present invention will be described below in detail with reference to the drawings. FIG. 1 is a perspective view for explaining a first configuration example of the solid-phase extraction device of the present invention, which is partially cut away. FIG. 2 is a cross-sectional view for explaining a first configuration example of the solid-phase extraction device of the present invention. The first configuration example includes a liquid feeding unit 20 (21, 22),
The cartridge 10 holding the filler 11 is made continuous,
The liquid sending means (2) is provided by the pressurizing means (30, 31, 32).
1, 2) and a structure in which pressure is applied to both ends of the cartridge 10.
【００１７】送液手段は、カートリッジ１０側の一端に
開口部２３を有する筒状体２２と、この筒状体２２内を
スライドするシリンダ状の押圧部２１を備えた注射器状
の構成とすることができる。試料液体や溶剤は筒状体２
０内に収納され、押圧部２１を開口部２３側に押すこと
によって、開口部２３から充填剤１１側に移動する。The liquid feeding means has a syringe-like configuration including a cylindrical body 22 having an opening 23 at one end on the cartridge 10 side, and a cylindrical pressing portion 21 that slides inside the cylindrical body 22. Can be. Sample liquid and solvent are cylindrical
When the pressing portion 21 is pressed toward the opening 23 side, the pressing portion 21 is moved from the opening 23 to the filler 11 side.
【００１８】充填剤１１は、Ｃ18，シリカ，フロリシ
ル，不活性けい藻土，アルミナ等の各種官能基等の固相
状の物質を用い、カートリッジ１０内に収納される。カ
ートリッジ１０の一方は開口部２３を通して筒状体２２
内と連通し、他方は開口部１２及び放出端１３を通して
外部と連通している。The filler 11 is contained in the cartridge 10 using a solid-phase substance such as C 18 , silica, florisil, inert diatomaceous earth, and various functional groups such as alumina. One of the cartridges 10 has a cylindrical body 22 through an opening 23.
The other communicates with the inside through the opening 12 and the discharge end 13.
【００１９】加圧手段は、駆動部３０と可動桿３１と支
持部３２を備える。可動桿３１は押圧部２１を充填剤１
１側に押すことによって筒状体２０内の溶液の圧力を高
める手段であり、駆動部３０によって動かされる。駆動
部３０は、可動桿３１を押圧する機構であれば手動，電
動、油圧、空圧等の任意の機構を採用することができ、
たとえば、ねじ機構により構成することができる。The pressurizing means includes a drive section 30, a movable rod 31, and a support section 32. The movable rod 31 presses the pressing portion 21 with the filler 1.
This is a means for increasing the pressure of the solution in the tubular body 20 by pushing it toward one side, and is moved by the drive unit 30. The drive unit 30 may employ any mechanism such as manual, electric, hydraulic, or pneumatic as long as the mechanism presses the movable rod 31.
For example, it can be constituted by a screw mechanism.
【００２０】又、支持部３２はカートリッジ１０の端部
を支持し、駆動部３０と共に、送液手段とカートリッジ
１０を両端から把持することによって、圧力の印加を行
う。The support section 32 supports the end of the cartridge 10, and applies pressure by gripping the liquid supply means and the cartridge 10 from both ends together with the drive section 30.
【００２１】なお、支持部３２の一部には切り欠き部分
３３を設け、この部分を通してカートリッジの放出端１
３を通して、送液手段及びカートリッジの設置を行うこ
とができる。A cut-out portion 33 is provided in a part of the support portion 32, and through this portion, the discharge end 1 of the cartridge is formed.
Through 3, the liquid supply means and the cartridge can be installed.
【００２２】保持手段４０は、筒状体２２の外周部分を
外側から被う筒体で構成することができ、筒状体２２を
外側から保持し、加圧による筒状体２２の変形を制限す
る強度を奏するものであれば、アルミニウム等の金属や
合成樹脂等の任意の素材を用いて形成することができ
る。この保持手段４０は、筒状体２２と別体で形成した
ものを挿入して取り付ける構成とすることも、又、筒状
体２２と一体で形成することもできる。The holding means 40 can be composed of a cylindrical body that covers the outer peripheral portion of the cylindrical body 22 from outside, and holds the cylindrical body 22 from outside to limit deformation of the cylindrical body 22 due to pressurization. It can be formed using an arbitrary material such as a metal such as aluminum or a synthetic resin as long as the material has high strength. The holding means 40 may be configured to insert and attach a member formed separately from the cylindrical body 22, or may be formed integrally with the cylindrical body 22.
【００２３】図３は、第１の構成例による固相抽出装置
の動作を説明するための概略図である。図３（ａ）にお
いて、筒状体内に蒸留水や所定の溶液を注入して、充填
剤内に送液し、充填剤内を所定の状態とするコンディシ
ョニングの処理を行う。次に、図３（ｂ）において、筒
状体内に試料液を注入し、加圧手段によって試料液を加
圧し、充填剤内に試料液を送液する。この加圧を行うこ
とによって、試料液中に微粒子状の物質やＳＳ分が含ま
れている場合や、多量の試料液であっても、送液を行う
ことができる。FIG. 3 is a schematic diagram for explaining the operation of the solid-phase extraction device according to the first configuration example. In FIG. 3A, a conditioning process is performed in which distilled water or a predetermined solution is injected into the cylindrical body, and is fed into the filler, so that the inside of the filler is in a predetermined state. Next, in FIG. 3B, the sample liquid is injected into the cylindrical body, the sample liquid is pressurized by the pressurizing means, and the sample liquid is sent into the filler. By performing this pressurization, the liquid can be sent even when the sample liquid contains fine particles or SS, or even when the sample liquid is a large amount.
【００２４】この後、図３（ｃ）において、分析成分以
外の夾雑物質を取り除くために、所定の溶剤を筒状体内
に注入し、再び、加圧手段によってこの溶剤を加圧して
充填剤内に送液する。これにより、充填剤からは夾雑物
質が取り残され、分析成分の分画を行うことができる。
次に、図３（ｄ）において、分析成分を取り出すため
に、所定の溶剤を筒状体内に注入し、再び、加圧手段に
よってこの溶剤を加圧して充填剤内に送液する。これに
より、充填剤からは分析成分が溶出し、分析成分の取り
出しを行うことができる。上記処理において、加圧時に
筒状体内の液体の圧力は高められるが、筒状体は保持手
段によって保持されているため変形は制限され、液漏れ
は発生しない。Thereafter, in FIG. 3 (c), in order to remove contaminants other than the components to be analyzed, a predetermined solvent is injected into the cylindrical body, and the solvent is again pressurized by the pressurizing means to thereby remove the filler. Solution. As a result, contaminants are left behind from the filler, and the analytical components can be fractionated.
Next, in FIG. 3D, a predetermined solvent is injected into the cylindrical body in order to remove an analysis component, and the solvent is pressurized again by the pressurizing means and sent into the filler. As a result, the analysis component is eluted from the filler, and the analysis component can be taken out. In the above process, the pressure of the liquid in the cylindrical body is increased at the time of pressurization, but since the cylindrical body is held by the holding means, deformation is limited, and no liquid leakage occurs.
【００２５】図４は本発明の固相抽出装置の第２の構成
例を説明するための斜視図である。第２の構成例は、送
液手段２０（２１，２２，２３）と、充填剤１１を保持
するカートリッジ１０とを分離し、加圧手段（３０，３
１，３２）によって送液手段（２１，２２）のみに圧力
を印加する構造とするものである。送液手段は、第１の
構成例と同様に、カートリッジ１０側の一端に開口部２
３を有する筒状体２２と、この筒状体２２内をスライド
するシリンダ状の押圧部２１を備えた注射器状の構成と
することができ、押圧部２１を開口部２３側に押すこと
によって、開口部２３から充填剤１１側に移動する。FIG. 4 is a perspective view for explaining a second configuration example of the solid phase extraction device of the present invention. In the second configuration example, the liquid feeding means 20 (21, 22, 23) and the cartridge 10 holding the filler 11 are separated, and the pressure means (30, 3) is separated.
1, 32) to apply pressure only to the liquid feeding means (21, 22). The liquid feeding means has an opening 2 at one end on the cartridge 10 side, as in the first configuration example.
3 and a syringe-shaped configuration including a cylindrical pressing portion 21 that slides in the cylindrical body 22. By pressing the pressing portion 21 toward the opening 23 side, It moves from the opening 23 to the filler 11 side.
【００２６】充填剤１１は第１の構成例と同様の物質を
用いることができ、筒状体２２と別体に形成したカート
リッジ１０内に収納される。カートリッジ１０の一方は
開口部２３に接続することによって筒状体２２内と連通
し、他方は放出端１３を通して外部と連通している。As the filler 11, the same substance as in the first configuration example can be used, and is contained in the cartridge 10 formed separately from the cylindrical body 22. One of the cartridges 10 communicates with the inside of the tubular body 22 by being connected to the opening 23, and the other communicates with the outside through the discharge end 13.
【００２７】加圧手段は、駆動部３０と可動桿３１と支
持部３２を備える。可動桿３１は、第１の構成例と同様
に、押圧部２１を充填剤１１側に押すことによって筒状
体２２内の溶液の圧力を高める手段であり、駆動部３０
によって動かされ、第１の構成例と同様の構成とするこ
とができる。又、支持部３２は筒状体２２の端部を支持
し、駆動部３０と共に、送液手段を両端から把持するこ
とによって、圧力の印加を行う。The pressurizing means includes a drive section 30, a movable rod 31, and a support section 32. The movable rod 31 is a means for increasing the pressure of the solution in the cylindrical body 22 by pushing the pressing part 21 toward the filler 11, as in the first configuration example.
And the same configuration as that of the first configuration example. The support section 32 supports the end of the cylindrical body 22 and applies pressure by gripping the liquid feeding means from both ends together with the drive section 30.
【００２８】保持手段４０は、筒状体２２の外周部分を
外側から被う筒体構成であり、第２の構成例では、加圧
手段側の支持部３２と連続して形成している。なお、保
持手段４０を形成する素材と同様とすることができる。The holding means 40 has a cylindrical structure that covers the outer peripheral portion of the cylindrical body 22 from the outside. In the second configuration example, the holding means 40 is formed continuously with the supporting portion 32 on the pressing means side. Note that the material may be the same as the material forming the holding means 40.
【００２９】なお、図４では、送液手段を保持手段に取
り付ける構成については示していないが、保持手段４０
の上方開口部分から送液手段を挿入する構成や、保持手
段４０の一部に切り欠き部分を設ける構成とすることが
できる。FIG. 4 does not show a structure in which the liquid feeding means is attached to the holding means.
The liquid feeding means can be inserted from the upper opening of the holding means, or a notch can be provided in a part of the holding means 40.
【００３０】図５は本発明の固相抽出装置の第３の構成
例を説明するための斜視図である。第３の構成例は、加
圧手段として、既存の接着剤や充填剤のチューブに用い
るコーキングガンと呼ばれる器具を転用した例である。FIG. 5 is a perspective view for explaining a third configuration example of the solid-phase extraction device of the present invention. The third configuration example is an example in which an existing device called a caulking gun used for a tube of an adhesive or a filler is diverted as the pressing means.
【００３１】抽出用注射器２１，２２をコーキングガン
３０に取り付け、押し出し棒３１によって、押圧部（シ
リンダ）２１を押し出して加圧を行う。又、抽出用注射
器の筒状体２２の外周部分に筒状の保持手段４０を取り
付ける。The extraction syringes 21 and 22 are attached to the caulking gun 30, and the pressing portion (cylinder) 21 is pushed out by the pushing rod 31 to apply pressure. Further, a cylindrical holding means 40 is attached to the outer peripheral portion of the cylindrical body 22 of the syringe for extraction.
【００３２】コーキングガン３０の一端には、一部に切
り欠き部分が形成された支持部３２が形成され、抽出用
注射器の端部を支持している。このコーキングガン３０
を用いる場合には、通常チューブを保持する部分に抽出
用注射器を設け、通常のコーキングガンの動作によっ
て、押し出し棒３１で押圧部（シリンダ）２１を押し出
して加圧を行うことができる。At one end of the caulking gun 30, there is formed a support part 32 having a cut-out part, which supports the end of the extraction syringe. This caulking gun 30
In the case of using, a syringe for extraction is usually provided in a portion for holding a tube, and the pressing portion (cylinder) 21 is pushed out by the push rod 31 by a normal operation of a caulking gun, so that pressurization can be performed.
【００３３】この第３の構成によれば、既存の器具を用
いて、固相抽出装置を構成することができる。According to the third configuration, a solid-phase extraction device can be configured using existing equipment.
【００３４】[0034]
【発明の効果】以上説明したように、本発明の固相抽出
装置によれば、微粒子状の物質が含まれる試料液やＳＳ
分を含む試料液や多量の試料液について、固相抽出を行
うことができる。As described above, according to the solid-phase extraction device of the present invention, the sample solution containing fine particles and the SS
Solid phase extraction can be performed on a sample solution containing a large amount or a large amount of a sample solution.
【図１】本発明の固相抽出装置の第１の構成例を説明す
るための、一部分を切り欠いた斜視図である。FIG. 1 is a partially cutaway perspective view for explaining a first configuration example of a solid-phase extraction device of the present invention.
【図２】本発明の固相抽出装置の第１の構成例を説明す
るための断面図である。FIG. 2 is a cross-sectional view for explaining a first configuration example of the solid-phase extraction device of the present invention.
【図３】本発明の第１の構成例による固相抽出装置の動
作を説明するための概略図である。FIG. 3 is a schematic diagram for explaining the operation of the solid-phase extraction device according to the first configuration example of the present invention.
【図４】本発明の固相抽出装置の第２の構成例を説明す
るための斜視図である。FIG. 4 is a perspective view for explaining a second configuration example of the solid-phase extraction device of the present invention.
【図５】本発明の固相抽出装置の第３の構成例を説明す
るための斜視図である。FIG. 5 is a perspective view for explaining a third configuration example of the solid-phase extraction device of the present invention.
【図６】従来の固相抽出の構成で高圧送液を行う場合を
説明するための断面図である。FIG. 6 is a cross-sectional view for explaining a case where high-pressure liquid sending is performed in a conventional solid-phase extraction configuration.
１０…カートリッジ、１１…充填剤、１２，２２、２３
…開口部、１３…放出端、２０…送液手段、２１…押圧
部、２２…筒状体、３０…駆動部、３１…可動桿、３２
…支持部、４０…保持手段。10: cartridge, 11: filler, 12, 22, 23
... Opening part, 13 ... Discharge end, 20 ... Liquid feeding means, 21 ... Pressing part, 22 ... Cylindrical body, 30 ... Drive part, 31 ... Movable rod, 32
... support part, 40 ... holding means.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.6 識別記号 ＦＩ Ｇ０１Ｎ 30/48 Ｇ０１Ｎ 30/48 Ｋ ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 6 Identification code FI G01N 30/48 G01N 30/48 K
Claims (1)
送液手段と、前記送液手段内の試料液を加圧する加圧手
段と、前記送液手段を保持する保持手段を備え、前記保
持手段は、前記送液手段の加圧による送液手段の変形を
制限する機能を備える固相抽出装置。1. A liquid feeding means for feeding a sample liquid to a filler for solid phase extraction, a pressure means for pressing a sample liquid in the liquid sending means, and a holding means for holding the liquid sending means. A solid-phase extraction device, wherein the holding unit has a function of restricting deformation of the liquid sending unit due to pressurization of the liquid sending unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9330312A JPH11160297A (en) | 1997-12-01 | 1997-12-01 | Solid phase extracting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9330312A JPH11160297A (en) | 1997-12-01 | 1997-12-01 | Solid phase extracting apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH11160297A true JPH11160297A (en) | 1999-06-18 |
Family
ID=18231236
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9330312A Withdrawn JPH11160297A (en) | 1997-12-01 | 1997-12-01 | Solid phase extracting apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH11160297A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008058207A (en) * | 2006-09-01 | 2008-03-13 | Sumika Chemical Analysis Service Ltd | Cartridge, and pretreatment apparatus for cadmium analysis having cartridge |
| WO2010026837A1 (en) * | 2008-09-05 | 2010-03-11 | 株式会社 日立ハイテクノロジーズ | Pretreatment device and mass spectrometer equipped with same |
| JP2013047680A (en) * | 2007-02-21 | 2013-03-07 | Brewer William | Pipette tips for extraction, sample collection and sample cleanup and methods for their use |
-
1997
- 1997-12-01 JP JP9330312A patent/JPH11160297A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008058207A (en) * | 2006-09-01 | 2008-03-13 | Sumika Chemical Analysis Service Ltd | Cartridge, and pretreatment apparatus for cadmium analysis having cartridge |
| JP2013047680A (en) * | 2007-02-21 | 2013-03-07 | Brewer William | Pipette tips for extraction, sample collection and sample cleanup and methods for their use |
| WO2010026837A1 (en) * | 2008-09-05 | 2010-03-11 | 株式会社 日立ハイテクノロジーズ | Pretreatment device and mass spectrometer equipped with same |
| US8926903B2 (en) | 2008-09-05 | 2015-01-06 | Hitachi High-Technologies Corporation | Pretreatment apparatus and mass spectrometer equipped with the same apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20050201 |