JPH11148919A - Capillary chip - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a capellary chip capable of analyzing samples different from each other under the same condition at the same time. SOLUTION: The first capillary flow path 41 ranging from a first opening 21 to a second opening 22 while successively passing first and second points 31, 32, the second capillary flow path 42 ranging from a third opening 23 to a fourth opening 24 while successively passing third and fourth points 33, 34, the third capillary flow path 43 reaching a fifth opening 25 from the first point 31 through the third point 33, the first introducing path 51 between the second point 32 to a sixth opening 26 and the second introducing path 52 between the fourth point 34 and a seventh opening 27 are formed in a transparent member 10. A first electrode 61 applying first potential to the first point 31 and the third point 33 and a second electrode 62 applying second potential to the second point 32 and the fourth point 34 are provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a capillary chip used for analyzing a very small amount of a sample in a buffer solution filled in a capillary channel by an electrophoresis method.

[0002]

2. Description of the Related Art Capillary electrophoresis has an inner diameter of several tens μm,
A buffer solution is filled into a hole of a capillary tube having a diameter of about several hundred μm and a length of several hundred mm, and a sample to be measured is introduced into the buffer solution. kV) is applied, the sample is electrophoresed, and the sample is analyzed by, for example, irradiating the sample with excitation light and detecting fluorescence generated by a photodetector.

[0003] The capillary tube is a thin glass tube, which is easily broken, and therefore it is not easy to handle, and it is difficult to fix the capillary tube to a detection unit or a temperature control tank. In addition, because of the cylindrical shape, the excitation light and the fluorescent light reach the photodetector via a complicated optical path, so that various stray lights also enter the photodetector and cause a detection error. In addition, Joule heat is generated by applying a voltage to both ends of the capillary tube, so it is necessary to perform temperature control to maintain the sample temperature constant. Not only is it not possible to cause a detection error, but also the temperature adjustment tank must be changed when changing the fluorescence extraction position, which is not easy to handle.

To solve such a problem, Japanese Patent Laid-Open No.
JP-A-259557 and JP-A-7-63727 propose a capillary chip in which a capillary is formed on a substrate by using photofabrication technology. According to this technology, a narrow groove having a rectangular cross section is formed on the surface of a substrate such as glass by using a photofabrication technique or the like, and another plate is laminated on the substrate, and the narrow groove and Is formed as a capillary channel.

[0005]

However, in each of the above-mentioned conventional capillary tubes and capillary tips, basically, there is only one capillary channel, and one sample can be prepared at a time under a certain condition. It is not possible to analyze different samples at the same time, just to analyze. If a plurality of capillary tubes or a capillary chip having a plurality of capillary channels are used, different samples can be analyzed simultaneously, but cannot be analyzed simultaneously under the same conditions. Therefore, relative evaluation between different samples cannot be performed with high accuracy, or absolute evaluation of a certain sample cannot be performed with high accuracy.

The present invention has been made to solve the above problems, and has as its object to provide a capillary chip capable of simultaneously analyzing different samples under the same conditions.

[0007]

According to the present invention, there is provided a capillary chip comprising: a transparent member having a first opening from a first opening to a second opening through a first point and a second point sequentially;
Flow path at the third point and the fourth point from the third opening
The second capillary flow path is sequentially passed through the points to the fourth opening, the third capillary flow path is passed from the first point to the fifth opening through the third point, and the second capillary flow path is the sixth to sixth openings. The first introduction path is formed before the first opening, and the second introduction path is formed between the fourth point and the seventh opening.

According to this capillary chip, the first to seventh openings are appropriately opened and closed, whereby the first to seventh openings are opened.
A buffer solution is filled into each of the first to third capillary channels from any one of the seventh openings, the first sample is introduced into the first capillary channel, and the second sample is introduced into the second capillary channel. Of samples may be introduced. And the first point and the third
When a voltage is applied between the point and the second and fourth points, the first capillary introduced into the first capillary channel is
Sample and the second sample introduced into the second capillary channel are electrophoresed simultaneously under the same conditions.

Further, in the capillary chip according to the present invention, a portion of the first capillary channel from the first point to the second point, and a portion of the second capillary channel from the third point to the fourth point. Are linear and parallel to each other. In this case, the first sample introduced into the first capillary channel and the second sample introduced into the second capillary channel are irradiated with the sheet-like excitation light from a predetermined direction. The fluorescence excited simultaneously and emitted from each of the first and second samples is detected simultaneously.

In the capillary chip according to the present invention, each of the first and second capillary channels has a rectangular cross section. In this case, the excitation light is applied to the sample without passing through a complicated path, and the fluorescence generated from the sample goes out without passing through a complicated path.

Further, in the capillary chip according to the present invention, the transparent member is made of quartz. In this case, even if the excitation light is ultraviolet light, the first and second samples are irradiated with high efficiency with little attenuation by the transparent member.

In the capillary chip according to the present invention, a first electrode for applying a first potential is provided at a first point and a third point of the transparent member, and a second potential is provided at a second point and a fourth point. Wherein a second electrode for providing the following is provided. In this case, by applying a voltage between the first electrode and the second electrode, the first sample introduced into the first capillary channel and the first sample introduced into the second capillary channel are introduced. The second sample is electrophoresed simultaneously under the same conditions.

[0013]

Embodiments of the present invention will be described below in detail with reference to the accompanying drawings. In the description of the drawings, the same elements will be denoted by the same reference symbols, without redundant description.

First, the configuration of the capillary chip 1 according to the present embodiment will be described with reference to FIGS.

FIG. 1 is a perspective view of a capillary chip 1 according to the present embodiment. In the capillary chip 1, capillary channels 41 to 43 and introduction channels 51 and 52 are formed inside a transparent member 10, and an electrode 61.
And 62. The transparent member 10 is made of a material that transmits both the excitation light to be irradiated to the sample introduced into the capillary channels 41 to 43 and the fluorescence generated from the sample. In particular, ultraviolet light generally used as the excitation light is used. Is preferably made of quartz that allows the light to pass through.

The first capillary flow path 41 is formed from the first opening 21 to the second opening 22 via the first point 31 and the second point 32. The second capillary channel 42 is formed from the third opening 23 to the fourth opening 24 via the third point 33 and the fourth point 34. The third capillary channel 43 is formed from the first point 31 to the fifth opening 25 via the third point 33. The first introduction path 51 is formed from the second point 32 to the sixth opening 26. Second introduction path 52
Are formed from the fourth point 34 to the seventh opening 27. The first capillary channel 41 is located at the first point 31.
, The third capillary channel 43 and the first introduction channel 51 at the second point 32 are connected to the respective channels. Further, the second capillary channel 42 includes a third capillary channel 43 at the third point 33 and a second introduction channel 52 at the fourth point 34.
And the flow paths are connected respectively.

At least a portion of the capillary channel 41 from the first point 31 to the second point 32 and a portion of the capillary channel 42 from the third point 33 to the fourth point 34 are each linear. Preferably, they are parallel to each other and of equal length. Further, the shape of the transparent member 10 is preferably a rectangular parallelepiped, and at least a portion from the first point 31 to the second point 32 of the capillary channel 41 and a portion from the third point 33 of the capillary channel 42 to the third point. The portion up to the four points 34 is formed parallel to any side of the rectangular parallelepiped.

The cross-sectional shape of each of the capillary channels 41 to 43 may be circular, but is preferably rectangular. If the cross-sectional shape is rectangular, the capillary channels 41 to 41
The excitation light applied to the sample introduced into each of the samples 43 and the fluorescence generated from the sample do not pass through a complicated optical path, so that the efficiency of excitation light irradiation and fluorescence detection is high. The cross-sectional dimension of each of the capillary channels 41 to 43 is, for example, about 50 μm to 100 μm square.

Each of the capillary channels 41 and 42 is a channel for electrophoresis of a sample to be analyzed, while each of the introduction channels 51 and 52 is a channel for introducing a buffer solution or a sample. It is. Each of the capillary channel 43 and the introduction channels 51 and 52 has one opening 2.
6, 27, not penetrating.

The first electrode 61 is connected to the first point 31 of the capillary channel 41 and the third point 33 of the capillary channel 42.
To give a first potential to the transparent member 10
, One end protrudes outside the transparent member 10, and the other end protrudes into the opening 25. The second electrode 62 applies a second potential to the second point 32 of the capillary channel 41 and the fourth point 34 of the capillary channel 42, and is partially embedded in the transparent member 10. , One end is outside the transparent member 10 and the other end is the second
It has reached the vicinity of the point 32, and the middle is the fourth point 34.
Passing nearby.

FIG. 2 is an explanatory view of the configuration of the capillary chip 1 according to the present embodiment, and is a cross-sectional view taken along three planes orthogonal to each other. FIG. 2 (a)
FIG. 2B is a cross-sectional view when cut along a first plane including first to third points 31 to 34, and FIG. 2B is perpendicular to the first plane and is parallel to the second point 32 and the fourth point. FIG. 2C is a cross-sectional view taken along a second surface including four points 34, and FIG.
FIG. 4 is a cross-sectional view taken along a third surface perpendicular to the first surface and including a third point 33 and a fourth point 34.

Next, an example of a method for manufacturing the capillary chip 1 according to the present embodiment will be described with reference to FIG.

First, the substrate 11, the upper plate 12, and the side plate 13
Prepare These are components to be the transparent member 10. The transparent member 10 is formed by bonding the upper plate 12 on the substrate 11 and bonding the side plates 13 to their side surfaces.

Upper surface of substrate 11 (surface to be bonded to upper plate 12)
Then, by etching, grinding, etc., the capillary channel 41
To 43 are formed, and the second point 32 is formed.
At the fourth point 34, a concave portion to be a part of the introduction path 52 is formed. In the upper plate 12, through holes that are to be a part of each of the introduction paths 51 and 52 are formed, and the electrodes 62 are embedded in the lower surface (the surface to be bonded to the substrate 11) by heating and melting.
The side plate 13 is formed with a through hole that is to be the opening 25 of the capillary channel 43, a through hole 61 </ b> A for passing the electrode 61 is formed, and the electrode 61 is attached.
Is formed to penetrate through the hole.

The positions of the two concave portions of the substrate 11 and the positions of the two through holes of the upper plate 12 are matched with each other so that the substrate 1
1 and the lower surface of the upper plate 12 are adhered. Next, the substrate 1
The side plate 13 is bonded to the side surfaces of the first and upper plates 12. At this time, the electrode 62 is made to penetrate through the through hole 62 </ b> A of the side plate 13, and the position of the end of the capillary channel 43 of the substrate 11 matches the position of the through hole of the side plate 13. At the time of bonding, these may be bonded using a transparent adhesive, or may be bonded by heating and melting. After bonding the substrate 11, the upper plate 12, and the side plate 13 in this manner, the respective surfaces around the transparent member 10 are polished and flattened.

Next, a first example of a method for introducing a sample or the like into the capillary chip 1 according to the present embodiment will be described with reference to FIG. The example described below is an example in the case of isoelectric focusing. All of the capillary channels 41 to 43 and the introduction channels 51 and 52 are filled with the amphoteric carrier to discharge air, and then the sample and the electrode solution are introduced.

The method for introducing a sample into the capillary channel 41 is as follows. Operate the valve 72 to operate the pump 9
2 and the opening 22 are connected. By operating the valve 71, the opening 21 and the waste liquid tank 81 are connected. Other valves 73-77
Each is operated to seal all of the openings 23 to 27.
Then, the sample is introduced into the capillary channel 41 from the opening 22 by the pump 92.

The method for introducing a sample into the capillary channel 42 is as follows. Operate the valve 74 to operate the pump 9
4 and the opening 24 are connected. By operating the valve 73, the opening 23 and the waste liquid tank 83 are connected. Other valves 71, 7
2, 75 to 77 to operate the openings 21, 22, 2
Seal all of 5-27. Then, the sample is introduced into the capillary channel 42 from the opening 24 by the pump 94.

The method for introducing the electrode solution into the capillary channel 41 on the electrode 62 side is as follows. The valve 76 is operated to connect the pump 96 and the opening 26. Valve 72
To connect the opening 22 to the waste liquid tank 82. Operate each of the other valves 71, 73 to 75, 77 to open
Seal all of 1,23 to 25,27. Then, the electrode solution is introduced into the introduction path 51 from the opening 26 by the pump 96.

The method of introducing the electrode solution into the capillary channel 42 on the electrode 62 side is as follows. The pump 77 and the opening 27 are connected by operating the valve 77. Valve 74
Is operated to connect the opening 24 and the waste liquid tank 84. Open each of the other valves 71 to 73, 75, and 76 by operating
Seal all of 1 to 23, 25 and 26. Then, the electrode solution is introduced from the opening 27 into the introduction path 52 by the pump 97.

The method for introducing the electrode solution to the electrode 61 is as follows. By operating the valve 75, the pump 95 and the opening 25 are connected. By operating the valve 71, the opening 21 and the waste liquid tank 81 are connected. Operate valve 73 to open 2
3 and the waste liquid tank 83 are connected. The other valves 72, 74,
By operating each of the openings 76, 77, the openings 22, 24, 26,
Seal all of 27. Then, the electrode liquid is introduced into the capillary channel 43 from the opening 25 by the pump 95.

Next, a second example of the method for introducing a sample or the like into the capillary chip 1 according to the present embodiment will be described with reference to FIG. The example described below is an example in the case of zone electrophoresis. All of the capillary channels 41 to 43 and the introduction channels 51 and 52 are filled with a buffer solution to discharge air, and then a sample is introduced.

The method for introducing a sample is as follows. By operating the valves 75 and 71, respectively, the pump 95, the opening 25, the capillary channel 43, the portion of the capillary channel 41 from the first point 31 to the opening 21, the opening 21
And the waste liquid tank 81 is connected. Other valves 72-74,
By operating each of 76 and 77, the openings 22 to 24, 26,
Seal all of 27. Then, the sample is introduced into the capillary channel 43 from the opening 25 by the pump 95.

In the case of zone electrophoresis, a third electrode 63 for applying a third potential is attached near the third opening 23, and a voltage is applied between the second electrode 62 and the third electrode 63. By doing so, the sample can be electrophoresed in the capillary channel 42. Here, the sample to be electrophoresed is a sample existing in a space where the capillary channel 42 and the capillary channel 43 intersect. Therefore, by appropriately designing the dimensions of the capillary channels 42 and 43, an appropriate amount of sample can be electrophoresed. Note that a fourth electrode (not shown) for applying a fourth potential may be attached near the first opening 21. In this case, a voltage is applied between the second electrode 62 and the fourth electrode. By applying the voltage, the sample can be electrophoresed also in the capillary channel 41.

Next, a method of analyzing a sample using the capillary chip 1 according to the present embodiment will be described.

First, if necessary (particularly in the case of separation by isoelectric focusing), the capillary channels 41 to 43 are used.
The inner wall is coated in the following procedure. The capillary channels 41 to 43 are filled with an aqueous solution of sodium hydroxide having a concentration of 100 mM, left at room temperature for 3 hours, and then ultrapure water is passed through the capillary channels 41 to 43 to remove the aqueous sodium hydroxide solution. Concentration 1 in the capillary channels 41 to 43
After filling with a 00 mM hydrochloric acid aqueous solution and left at room temperature for 10 minutes, ultrapure water is passed through the capillary channels 41 to 43 to remove the hydrochloric acid aqueous solution. Capillary channels 41-43
Was filled with a 0.3% aqueous solution of 3-methacryloxypropyltrimethoxysilane-60% acetone.
Leave at ℃ for 24 hours. Then, a 2% concentration acrylamide (monomer) -0.05% N, N, N ', N'-tetramethylethylenediamine aqueous solution was degassed under reduced pressure while stirring with a rotor, and a 10% concentration aqueous ammonium persulfate solution was added thereto. To a final concentration of 0.05%, and after short-time stirring, quickly fill the capillary channels 41 to 43, leave at room temperature for 1 hour, and pass ultrapure water through the capillary channels 41 to 43. Wash. Until use, immerse in 0.05% aqueous sodium azide solution and store at 4 ° C.

Subsequently, the capillary channels 41 to 43 and the introduction channels 51 and 52 are filled with a buffer solution. The buffer solution to be filled here is appropriately selected according to the electrophoresis separation mode to be employed. In the case of separation by zone electrophoresis, for example, a concentration of 20 mM to 100 m
A phosphate buffer, a borate buffer or a Tris-acetate buffer is selected in the range of M and pH 2 to 12, and is suitably used as a buffer. In the case of separation by electrokinetic chromatography, for example, sodium dodecyl sulfate (SDS) is used as a surfactant at a concentration of 50%.
50 mM borate buffer (pH 8.5-9.
0) is suitably used as a buffer solution. In the case of separation by gel electrophoresis, for example, a solution containing a gel of polyacrylamide is suitably used as a buffer solution.
Preferably, the inner wall of 43 is chemically modified.

In the case of separation by isoelectric focusing,
For example, a solution containing an amphoteric carrier is suitably used as a buffer solution. If the inner walls of the capillary channels 41 to 43 filled with the solution containing the amphoteric carrier are chemically decorated in advance with polyacrylamide, the generation of electroosmotic flow is suppressed, and separation by isoelectric focusing can be suitably performed. The addition of methylcellulose to the solution containing the amphoteric carrier also suppresses the generation of electroosmotic flow, and can suitably perform separation by isoelectric focusing. On the anode side of the capillary channels 41 and 42 filled with the solution containing the amphoteric carrier, for example, a concentration of 10 mM
~ 100 mM phosphoric acid aqueous solution, for example, at a concentration of 1
By filling each with an aqueous solution of sodium hydroxide of 0 mM to 100 mM, separation by isoelectric focusing can be suitably performed. Also, when methylcellulose is added to the solution containing the amphoteric carrier, by adding methylcellulose to each of the phosphoric acid aqueous solution on the anode side and the sodium hydroxide aqueous solution on the cathode side, the specific gravity and the viscosity of each solution become substantially the same, The respective solutions are hardly mixed, and separation by isoelectric focusing can be suitably performed.

Hereinafter, the separation by the isoelectric focusing method will be described. In each of the capillary channels 41 and 42, 25-fold diluted fermalite (pH 3-10) -0.
Fill with 4% hydroxypropyl methylcellulose (2% solution having a viscosity of 4000 cP at 25 ° C.)-5 mM arginine solution. Thereafter, the first capillary channel 41
Is filled with the same solution in which the first fluorescent sample is dissolved, and the second capillary channel 42 is filled with the same solution in which the second fluorescent sample is dissolved. A 20 mM aqueous phosphoric acid solution containing 0.5% hydroxypropylmethylcellulose is used as an anolyte, and a 20 mM aqueous sodium hydroxide solution containing 0.5% hydroxypropylmethylcellulose is used as a catholyte.

Then, a voltage is applied between the electrodes 61 and 62, and a potential gradient of 200 to 500 V / cm and a current of 6 to 15 μA are applied to the capillary channels 41 and 42, respectively.
give. The first point 31 of the capillary channel 41 and the third point 33 of the capillary channel 42 have the same potential, and the second point 32 of the capillary channel 41 and the fourth point 34 of the capillary channel 42 are also equal. And the length of the portion of the capillary channel 41 from the first point 31 to the second point 32 is equal to the length of the portion of the capillary channel 42 from the third point 33 to the fourth point 34. The potential gradient of the portion from the first point 31 to the second point 32 of the capillary channel 41 and the potential gradient of the portion from the third point 33 to the fourth point 34 of the capillary channel 42 are equal to each other.

By irradiating the capillary chip 1 with excitation light, the first fluorescent sample in the capillary channel 41 and the second fluorescent sample in the capillary channel 42 are excited, and the fluorescent light generated thereby is excited. Observe. In addition, as excitation light,
For example, a laser beam with a wavelength of 532 nm output from a laser diode-pumped Nd: YAG laser light source is preferably used. Further, by using an optical system such as a cylindrical lens or the like, the excitation light is converted into sheet light (a parallel light having a substantially linear cross section), and the excitation light is incident along a plane including the first point 31 to the fourth point 34. Then, the capillary channel 41
It is preferable to simultaneously irradiate the portion between the first point 31 and the second point 32 and the portion between the third point 33 and the fourth point 34 of the capillary channel 42 with excitation light. Further, it is preferable to detect fluorescence two-dimensionally from a direction perpendicular to a plane including the first point 31 to the fourth point 34.

By doing so, the first fluorescent sample and the second fluorescent sample introduced into the first capillary channel 41 are formed.
The second fluorescent sample introduced into the capillary channel 42 of the above can be subjected to electrophoresis under the same conditions as each other and analyzed simultaneously. Therefore, the first fluorescent sample and the second fluorescent sample
Highly accurate relative evaluation between the fluorescent sample and the fluorescent sample is possible. Further, for example, if a standard sample having a known pH value is used as the second fluorescent sample, the first fluorescent sample can be absolutely evaluated with high accuracy.

Although the case of separation by isoelectric focusing has been described above, the first fluorescent sample is similarly introduced into the first capillary channel 41 in the case of separation by other electrophoresis. Then, by introducing a second fluorescent sample (or a standard sample) into the second capillary channel 42,
A high-precision relative evaluation between different samples is possible, or a high-precision absolute evaluation of the samples is also possible.

The present invention is not limited to the above embodiment, but can be variously modified. For example, the arrangement of the electrodes is not limited to that described in the above embodiment. The first potential is applied to the first point 31 and the third point 33, and the second potential is applied to the second point 32 and the fourth point 34. Any electrode arrangement may be used as long as a potential can be applied.

In the above embodiment, the case where the number of parallel capillary channels is two has been described. However, the present invention can be easily extended to the case where there are three or more channels. In general, if there are n parallel capillary channels, highly accurate relative evaluation among n types of samples is possible, or n-1
High-precision absolute evaluation of each type of sample is possible.

[0046]

As described above in detail, according to the present invention, by appropriately opening and closing the first to seventh openings, the first to seventh openings can be moved from any of the first to seventh openings. Third
Each of the capillary channels may be filled with a buffer solution, the first sample may be introduced into the first capillary channel, and the second sample may be introduced into the second capillary channel. Then, when a voltage is applied between the first point and the third point and the second point and the fourth point, the first sample introduced into the first capillary channel and the second capillary channel are introduced. Are electrophoresed simultaneously under the same conditions. Therefore, a highly accurate relative evaluation between the first and second samples is possible. Also, for example, if a standard sample having a known pH value is used as the second sample, the first sample can be absolutely evaluated with high accuracy.

Further, the portion from the first point to the second point of the first capillary channel and the third portion of the second capillary channel
When the portion from the point to the fourth point is linear and parallel to each other, the sheet-like excitation light was irradiated from a predetermined direction to be introduced into the first capillary channel. The first sample and the second sample introduced into the second capillary channel are simultaneously excited, and
And the fluorescence generated from each of the second sample is detected simultaneously. Further, the optical system for irradiating the excitation light and the optical system for detecting the fluorescence have simple configurations.

When the cross-sectional shape of each of the first and second capillary channels is rectangular, the excitation light is applied to the sample without passing through a complicated path, and the fluorescent light generated from the sample passes through the complicated path. Go outside without going through. Therefore, each of the first sample introduced into the first capillary channel and the second sample introduced into the second capillary channel is irradiated with the excitation light with high efficiency, and the first and second samples are introduced. Fluorescence generated from each sample is detected with high efficiency.

When the transparent member is made of quartz,
Even if the excitation light is ultraviolet light, the first and second samples are irradiated with high efficiency without being substantially attenuated by the transparent member, so that the intensity of the fluorescence generated from the sample is large.

A first electrode for applying a first potential is provided at a first point and a third point of the transparent member, and a second electrode for applying a second potential is provided at a second point and a fourth point. When the voltage is applied between the first electrode and the second electrode, the first sample introduced into the first capillary flow path and the second capillary flow path are applied to the first capillary flow path. The introduced second sample is electrophoresed simultaneously under the same conditions.

[Brief description of the drawings]

FIG. 1 is a perspective view of a capillary chip according to an embodiment.

FIG. 2 is an explanatory diagram of a configuration of a capillary chip according to the present embodiment.

FIG. 3 is an explanatory diagram of a method for manufacturing a capillary chip according to the embodiment.

FIG. 4 is an explanatory diagram of a method for introducing a sample or the like into a capillary chip according to the present embodiment.

FIG. 5 is an explanatory diagram of a method for introducing a sample or the like into a capillary chip according to the present embodiment.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Capillary chip, 10 ... Transparent member, 11 ... Substrate,
12 ... upper plate, 13 ... side plate, 21-27 ... opening, 31 ... first point, 32 ... second point, 33 ... third point, 34 ... fourth
Points, 41 to 43: Capillary flow path, 51, 52: Introduction path, 61 to 63: Electrode, 71 to 77: Valve, 81 to 8
4, 86, 87: waste liquid tank, 92, 94 to 97: pump.

Claims (5)

[Claims] A first opening through a first opening inside the transparent member;
The first capillary flow path sequentially passes through the point and the second point to the second opening, and the second capillary flow path passes from the third opening to the fourth opening through the third and fourth points sequentially. From the first point to the fifth point via the third point.
A third capillary channel between the second point and the sixth opening, a first introduction path between the second point and the sixth opening, and a second capillary path between the fourth point and the seventh opening. A capillary chip, wherein each of the introduction paths is formed. 2. A part of the first capillary channel from the first point to the second point, and a part of the second capillary channel from the third point to the fourth point, The capillary chip according to claim 1, wherein each of the capillary chips is linear and parallel to each other. 3. The capillary chip according to claim 1, wherein each of the first and second capillary channels has a rectangular cross section. 4. The capillary chip according to claim 1, wherein said transparent member is made of quartz. 5. A first electrode for applying a first potential to the first point and the third point of the transparent member,
2. The capillary chip according to claim 1, wherein a second electrode that applies a second potential is provided at the second point and the fourth point. 3.