JPH11127867A - Cellulose synthetase gene - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new cellulose synthetase gene useful for a study aiming at insertion thereof into microalgae and useful for production of bacterial cellulose, etc., derived from acetic acid bacteria, and above all to obtain a gene relating to synthesis of acylated cellulose. SOLUTION: This gene comprises the base sequence of the formula which is composed of bases from No.9207 to No.10364 in the DNA fragment having 17091 base pairs containing almost all of cellulose synthetase gene prepared by cloning a part of cellulose synthetase gene of a genome library derived from the strain of Acetobacter xylinum JCM No.7664 (IFO No.13693) through a well-known method.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

If the CO ₂ is said to be one of the causes of global warming BACKGROUND OF THE INVENTION The absorption using microalgae, conventional organic carbon fixed is often return to CO ₂ by decomposition, a large amount of CO _{In order} to fix ₂ , it is desired to use cellulose which is a chemically stable substance having a long life cycle. The present invention is a study for synthesizing cellulose into microalgae for the purpose of effectively utilizing CO ₂ ,
That is, the present invention relates to isolation of a cellulose synthase gene derived from acetic acid bacteria, which is positioned as a part of research for incorporating a cellulose synthase gene into microalgae.

[0002]

2. Description of the Related Art It has long been reported that acetic acid bacteria produce cellulose. Cellulose produced by this bacterium, unlike vegetable cellulose, is a high-purity cellulose that does not contain any hemicellulose or lignin, and is an ultra-fine fiber that is about 1/1000 of cotton or flax fiber. Due to its high cost, research on its practical use is underway.

[0003] Bacteria producing such bacterial cellulose include, in addition to the acetic acid bacterium Acetobacter, Achromobacter and Aerobacter.
erobacter, Agrobacterium (Agro)
bacterium), Alcaligenes (Alcali)
geneses), Azotobacter
r), Rhizobium, Pseudomonas, Sarcina (Sarc)
Ina) and the like, among which Acetobacter xylinum (Acetobacter xylin)
um) is attracting attention. However, it was not known that these bacteria produce acylated cellulose.

The cloning of a cellulose synthase gene derived from Acetobacter xylinum has already been reported, and the sequence of four genes (bcsA / B / C / D) of Acetobacter xylinum strain 1306-3 (Proc.
USA, 87, 8130, (1990)) and the sequence of three genes (acsAB / C / D) of Acetobacter xylinum ATCC 53582 (Plant Mol. Biol., 1).
6,947, (1991) and JOURNAL OF BACTERIOLOGY, Sept. 1994,
p.5735-5752) and Acetobacter xylinum A
Y201 strain cellulose synthase gene (acsAI
I) sequence (JOURNAL OF BACTERIOLOGY, Sept. 1995, p. 527)
6-5283). And
Since the gene (acsAII) of the Acetobacter xylinum AY201 strain is called a type II cellulose synthase gene, a gene having a high homology to this acsAII is hereinafter referred to as a type II cellulose synthase gene. The gene of the 1306-3 strain (bcsA / B / C / D) and the gene of the Acetobacter xylinum ATCC53582 strain (acsAB / C /
D) and bcsA / B / C / D or acsAB / C / D
Hereinafter, those having a high homology will be referred to as type I cellulose synthase genes.

Among the cellulose synthase genes, b
csA and bcsB are thought to be genes encoding the subunits BcsA and BcsB of cellulose synthase, respectively, and the single gene acsAB also has a molecular weight of 168.
It is considered to be a gene encoding a cellulose synthase synthesized as a kDa polypeptide AcsAB. Both BcsA and BcsB polypeptides are homologous to the N-terminal half and the C-terminal half of polypeptide AcsAB, respectively. acsC and bcsC and acsD and bcsD are genes required for the production of cellulose in vivo, respectively, but the exact functions of the polypeptides encoded by these genes are not well understood at present.

[0006] Further, as can be seen from the fact that production of acylated cellulose by bacteria was not known, the existence of genes involved in cellulose acylation was not known. However, the gram-negative bacteria Salmonella typhimurium (Salmonella)
The O antigen present on the outermost side of the lipopolysaccharide encoding the surface of T. typhimurium is acetylated, and the protein Oa encoded by the gene oafA involved in the modification of the subunit of this O antigen is
fA has been reported to have regions of homology to many prokaryotic and eukaryotic acylating enzymes (JOURNAL
OF BACTERIOLOGY, Oct. 1996, p.5904-5909).

[0007]

SUMMARY OF THE INVENTION An object of the present invention is to isolate a novel cellulose synthase gene derived from acetic acid bacteria, particularly a gene involved in the synthesis of acylated cellulose, and to provide a gene DNA thereof.

[0008]

Means for Solving the Problems In the course of conducting various studies on the bacterial cellulose synthase gene from acetic acid bacterium, the present inventors have discovered a novel, previously unknown strain of Acetobacter xylinum JCM7664. The present inventors have found a cellulose synthase gene, particularly a gene involved in the synthesis of acylated cellulose, and have completed the present invention.

That is, the present invention relates to a nucleotide sequence represented by SEQ ID NO: 11, in particular, a nucleotide sequence from nucleotides 9206 to 10364 of the nucleotide sequence represented by SEQ ID NO: 11,
The nucleotide sequence from the 3745th position to the 8298th position and 10
DNAs represented by the nucleotide sequences from the 375th to 14295th nucleotides, their complementary sequences and DNAs containing a part or all of these sequences, and DNAs derived from acetic acid bacteria which hybridize with these DNAs under stringent conditions D
NA or a DNA encoding a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 12, 13 or 14, or one or several amino acids in the amino acid sequence shown in SEQ ID NO: 12, 13 or 14 are deleted, substituted or The present invention relates to a DNA encoding a polypeptide consisting of an added amino acid sequence. Here, the stringent conditions in "hybridize under stringent conditions" refer to
It refers to conditions in the sense commonly used by those skilled in the art.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION

(Microorganism used) In order to obtain a cellulose synthase gene, in the present invention, Acetobacter xylinum J
The CM7664 strain (same as IFO13693 strain) was used. Acetobacter xylinum JCM7664 strain
JCM (JAPANCOLLECTION OF MIC)
ROORGANISMS, RIKEN; RIKEN Microbial Strain Preservation Facility).

(Extraction of Genomic DNA) Acetobacter
A known method can be used for extracting genomic DNA of Xylinum JCM7664 strain.
Since xylinum grows in a culture solution while producing cellulose outside the body, acetic acid bacteria are obtained as a large soul entangled in a cellulose membrane by a normal culture method. In this state, it is difficult to extract the gene in the cells by crushing the cells, so it is desirable to add the cellulase to the culture solution and culture. After culturing, the culture solution is centrifuged, and the collected cells are treated with cell wall hydrolase and protein hydrolase to dissolve the cell walls and inactivate DNA and RNase. Genome D
NA can be obtained.

(Cloning of Cellulose Synthase Gene) In order to isolate a cellulose synthase gene from Acetobacter xylinum JCM7664 strain, a conventionally known gene cloning method can be used. It is preferable to use a known nucleotide sequence of the gene. For example, Acetobacter
Xylinum strain 1306-3 and / or Acetobacter
Using an oligonucleotide synthesized based on the known base sequence of the cellulose synthase gene of Xylinum ATCC 53582, Acetobacter xylinum JCM was used by the PCR (polymerase chain reaction) method.
The corresponding gene portion of the 7664 strain was amplified, the amplified DNA was cloned using a plasmid vector, and the nucleotide sequence was determined by a conventional method after cloning.
By comparing with the nucleotide sequence of a known cellulose synthase gene, Acetobacter xylinum JCM766
It is confirmed that a part of the cellulose synthase gene of No. 4 could be isolated.

Next, a DNA identified as a part of the cloned cellulose synthase gene was labeled with an antibody, an enzyme or the like and used as a probe, and the genomic DNA of Acetobacter xylinum JCM7664 strain was determined by Southern blotting.
Is analyzed. As a result, multiple DNA
When a fragment is detected, it can be estimated that a plurality of cellulose synthase genes are present in the Acetobacter xylinum JCM7664 strain. Therefore, the detected DNA fragment is incorporated into a plasmid vector by a conventional method, and colony hybridization is performed. A positive clone is obtained by the method. The genomic DNA of this positive clone is subjected to mapping with various restriction enzymes, and if necessary, by determining a partial base sequence, it is confirmed whether or not it contains almost the entire target cellulose synthase gene. .

When the obtained DNA fragment is a part of the target cellulose synthase gene,
A part of was subcloned using a restriction enzyme, an antibody, a probe labeled with an enzyme or the like in the same manner as described above,
Acetobacter xylinum J by Southern blot
The genomic DNA of the CM7664 strain is analyzed, and a DNA fragment expected to contain the target gene is incorporated into a plasmid vector based on the results of the detection, and isolated by colony hybridization to obtain a positive clone. The genomic DNA of this positive clone is subjected to mapping with various restriction enzymes, and if necessary, by determining a partial base sequence, it is confirmed whether or not it contains almost the entire target cellulose synthase gene. . Through the above operations, the cellulose synthase gene can be cloned.

[0015]

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples. Example 1 (Extraction of genomic DNA) Acetobacter xylinum J
The CM7664 strain was prepared using the cellulase-added GYP shown in Table 1 below.
The medium was shake-cultured at 30 ° C. for 6 days in a medium (culture solution). 600 ml of the culture solution was centrifuged, and 1.2 g (f
r wt. ) Was treated with lysozyme and proteinase K to obtain 22.0 mg of genomic DNA of Acetobacter xylinum JCM7664 strain.

[0016]

[Table 1]

(Cloning of Type I Cellulose Synthase Gene) Isolation of the cellulose synthase gene from Acetobacter xylinum JCM7664 strain was performed using cellulose synthases derived from Acetobacter xylinum strain 1306-3 and Acetobacter xylinum ATCC 53582, respectively. The oligonucleotide A sense primer represented by SEQ ID NO: 1 and the oligonucleotide represented by SEQ ID NO: 2 synthesized based on the known base sequence of the common part between the genes bcsA and acsAB and the common part between bcsC and bcsD and acsC and acsD Using the oligonucleotide A anti-primer, the oligonucleotide C sense primer shown in SEQ ID NO: 3 and the oligonucleotide D anti-primer shown in SEQ ID NO: 4, It was amplified relevant gene portion of the terpolymer xylinum JCM7664 strain.

The nucleotide sequence of each DNA obtained by amplification by the PCR method was determined by a conventional method. The 641 bp PCR product shown in SEQ ID NO: 5 obtained using the A sense primer and the A anti-primer and the 421 bp PCR product shown in SEQ ID NO: 6 obtained using the C sense primer and the D anti primer were Four genes of the Acetobacter xylinum strain 1306-3 (bcsA / B /
C / D) and Acetobacter xylinum ATCC 53
Comparison with the three genes (acsAB / C / D) of the 582 strain revealed that each of the cellulose synthase genes bcsA
And acsAB, and a part of a common part between bcsC and bcsD and acsC and acsD. That is, it was confirmed that a part of the cellulose synthase gene of Acetobacter xylinum JCM7664 strain could be isolated.

The DNA of 641 bp shown in SEQ ID NO: 5 and 4 shown in SEQ ID NO: 6 obtained by the PCR method
A 21 bp DNA was labeled with [α- ³² P] dCTP and used as a probe. 641 bp DN shown in SEQ ID NO: 5
Six positive clones were obtained from a cosmid library (30 kb) of Acetobacter xylinum JCM7664 strain (cosmid vector was pWE15) by a colony hybridization method using the A probe. Colony hybridization using the 421 bp DNA probe shown in SEQ ID NO: 6 was performed on these six positive clones to obtain four positive clones. The nucleotide sequence of the clone having the smallest size was analyzed, and the entire nucleotide sequence including the cellulose synthase gene was determined. 1 represented by this SEQ ID NO: 7
The DNA having the nucleotide sequence of 2065 bp includes the type I cellulose synthase gene bcsA /
A portion corresponding to B / C / D was confirmed, and this gene
Type cellulose synthase gene. Therefore, a type I cellulose synthase gene newly isolated from Acetobacter xylinum JCM7664 strain was
BcsAI / bcsBI / bcsCI / bcs respectively
DI. Of these, bcsAI of the nucleotide sequence shown in SEQ ID NO: 7 is the nucleotide sequence from the 2125th position to the 4359th position, and bcsBI is the nucleotide sequence of the 4364th to 677th position.
The nucleotide sequence up to the fifth, bcsCI is the nucleotide sequence from the 6781th to the 10755th, bcsDI is 1075
It is represented by the nucleotide sequence from the 8th to the 11225th.

In the homology comparison of amino acids assumed from these nucleotide sequences, bcsAI, bcsBI, bcs
In all regions of CI and bcsDI, the corresponding cellulose synthase genes bcsA, bcsB, bcsC and bc derived from Acetobacter xylinum 1306-3 strain
Similar to sD, their homology is 81% and 8% respectively.
They were 3%, 86% and 94%. The operon structure also had a boundary in the AB region, as in the 1306-3 strain. BcsAI, bcsBI, bcsCI, bc
sDI and Acetobacter xylinum ATCC 535
Corresponding cellulose synthase gene acs from strain 82
The homology with A (B), acs (A) B, acsC and acsD was 67%, 60%, 64% and 78%, respectively.

The DNA represented by SEQ ID NO: 7 includes
It was found that three open reading frames (ORFs) exist around the type I cellulose synthase gene. An ORF having a C-terminal truncation homologous to β-glucosidase was confirmed in the downstream region. In addition, in the upstream region, a truncated ORF homologous to endoglucanase,
It was found that ORF (346) homologous to cellulose complementing protein (ccp), which is considered essential for cellulose synthesis. In comparison of the amino acid sequence of ccp assumed from the nucleotide sequence shown from the 945th position to the 1931th position in the nucleotide sequence of SEQ ID NO: 7, the type I cellulose synthase gene derived from the present Acetobacter xylinum 7664 strain was It turned out to be similar to that derived from Acetobacter xylinum strain 1306-3.

(Cloning of Type II Cellulose Synthase Gene) The oligonucleotide A 'sense primer shown in SEQ ID NO: 8 corresponding to a part of bcsA used for cloning the type I cellulose synthase gene and SEQ ID NO: 9 Using the indicated oligonucleotide A 'anti-primer, the Acetobacter
The relevant gene portion of Xylinum JCM7664 was amplified. The amplified DNA was labeled with an antibody and used as a probe to analyze the genomic DNA of Acetobacter xylinum JCM7664 strain by Southern blotting. As a result, a plurality of DNA fragments were detected by digestion with various restriction enzymes.
It was presumed that the M7664 strain had a plurality of cellulose synthase genes. The approximately 8 kbp DNA fragment obtained by co-digestion of SalI / BamHI was the largest fragment having another restriction enzyme site at both ends, and was inserted into a plasmid vector (pUC18) to perform colony hybridization. By law 1
Eight positive clones were obtained.

Mapping of these 18 positive clones with various restriction enzymes revealed that the 18 strains were divided into two types of clones. That is, when its base sequence was partially determined,
Both types, bcsA and bc of cellulose synthase genes
It was confirmed that it contained a gene corresponding to almost the whole of sB and no gene downstream thereof corresponding to bcsC and bcsD, but the upstream region of the gene corresponding to bcsA was completely different between the two clones. . Therefore, these two types of clones were respectively called pCEL1 and pCEL1.
2, and their restriction maps are shown in FIG.

Next, SalI / X of pCEL1 was isolated in order to isolate genes corresponding to downstream bcsC and bcsD.
The hoI fragment (shaded in FIG. 1) was subcloned and the nucleotide sequence was determined. This 446 shown in SEQ ID NO: 10
The bp base sequence was labeled with an antibody in the same manner as described above and used as a probe, and genomic DNA was analyzed by Southern blotting. Unlike the case where a part of the gene corresponding to bcsA amplified by the PCR method was used as a probe, a single fragment was detected with most of the restriction enzymes. However, Hin
The dIII / KpnI co-digestion resulted in two fragments.
This suggests that two regions of similar sequence are contained in the genome.

From these results, the desired bcsC, bc
Hin expected to contain the gene corresponding to sD
An approximately 8 Kbp fragment of dIII was inserted into a plasmid vector (pU
C18) and isolated by colony hybridization to obtain four positive clones. When mapping was performed using various restriction enzymes, all of the clones were considered to be a single clone, and this was designated as pCEL3. When a part of the nucleotide sequence of this pCEL3 DNA was determined, it was completely identical to a part of pCEL1 and was slightly different from pCEL2. Since pCEL1 and pCEL3 are considered to be derived from the same region, their base sequences were determined by a conventional method, overlapping portions of both sequences were deleted, and the original sequence was reproduced. The regenerated DNA fragment was 13088
Although it had bp bases, it was considered that the downstream portion of the gene corresponding to bcsC was missing, so the gene corresponding to full-length bcsC was obtained as follows.

Using the SmaI / BamHI fragment of pCEL3 (533 bp) as a probe, hybridization was carried out with a cosmid library of the acetic acid bacterium Acetobacter xylinum JCM7664 strain, which was also used for cloning of the type I cellulose synthase gene, to obtain 30 kDa.
Two positive clones of b were obtained (cosmid vector was pWE15). One of these clones (pCE
When a part of the base sequence of L4) was determined, pCEL1
And a part of the nucleotide sequence of the other clone (pCEL5) was completely determined, and it was found to be completely consistent with a part of pCEL2. From these facts, pCEL4 was converted to pCEL1 and pCEL3
Therefore, the nucleotide sequence of about 12 kb upstream of pCEL4 was determined by a conventional method. As a result, pCEL4 contained a part of pCEL1, the full length of pCEL3, and further contained the downstream of pCEL3. Duplicates of these sequences were deleted and regenerated to the original sequence. The regenerated DNA fragment represented by SEQ ID NO: 11 was 17091
bp base (see FIGS. 2 to 10).

As described above, pCEL1 and pCEL2 are very similar in the bcsA and bcsB gene regions, but completely different in the upstream region, and the boundary region between the homologous region and the different region is defined by various restriction enzymes. The analysis was carried out by mapping with a computer and determining a part of the nucleotide sequence. As can be seen from FIG. 11, immediately upstream of methionine (ATG),
Since there is a stop codon (TAG) in the same reading frame, this methionine was found to be the translation start position of the gene corresponding to bcsA (the same base is marked with * in FIG. 11). The homology region of both pCEL1 and pCEL2 was from the translation start position of the gene corresponding to bcsA to 13 bp upstream, but the region further upstream was clearly different between the two.

The common part of the two clones pCEL1 and pCEL2 is a homologue of the AB region of the type I cellulose synthase gene, and is represented by bcsABII-A and bcs, respectively.
It was named ABII-B. These had 99% homology in both base sequence and amino acid sequence. In contrast, as described above, the upstream region of bcsABII is composed of pCEL1 and pCEL1.
This is quite different from CEL2, suggesting that the genome of Acetobacter xylinum strain 7664 contains at least two or more very similar cellulose synthase genes. Also, bcsABII-
A transposase (ORF448) well-known in acetic acid bacteria is present upstream of type B, and two ORFs of unknown function (ORF200, ORF200) are present upstream of bcsABII-A type.
F265) and a homolog of melibiose carrier protein (N-terminal is missing).

On the other hand, in the base sequence of SEQ ID NO: 11, downstream of bcsABII-A represented by the base sequence from position 3745 to position 8298, positions 8334 to 9119 and 9207 to 10364 Through two ORFs shown by the base sequence from the base sequence to the ORF corresponding to the bcsC region shown by the base sequence from the 10375th position to the 14295th position;
It was found that another ORF represented by the nucleotide sequence from 332 to 16038 was present. These two intervening ORFs are named bcsX and bcsY, respectively, and the ORs corresponding to the bcsC region
F was also named bcsCII, and the ORF downstream thereof was ORF (569) (see FIG. 12).

BcsABII-A represented by SEQ ID NO: 12
Is compared with the expected amino acid sequence of the type I gene, it shows only 33% homology with BcsA and BcsB and 31% with AcsAB, showing relatively low homology with the type I gene. However, in response to this, BROWN et al.
66% compared to the amino acid sequence of acsAII of type II gene
And a high homology clearly different from the type I gene. Therefore, this bcsABII-A is the II of the present 7664 strain.
It was concluded that this was a type of gene.

Next, when the assumed amino acid sequence of bcsCII consisting of 1307 amino acid residues represented by SEQ ID NO: 14 is similarly compared with the assumed amino acid sequence of the type I gene, it is found that BcsCI and BcsC have 31% A
30% with csC, as in the AB region, showed relatively low homology with the type I gene.

The two ORFs sandwiched between bcsABII-A and bcsCII and the ORF downstream of bcsCII are shown in FIG. 12 as bcsX (ORF 262), bcs
Indicated as sY (ORF386) and ORF (569). Of these, the functions of bcsX and ORF (569) are not well understood, but bcsY has homology with transacylase. This bcsY corresponds to almost the entire area of the transacylase region in the oafA gene of Salmonella, and based on the assumed amino acid sequence BcsY of bcsY represented by SEQ ID NO: 13, BcsY
FIG. 13 also shows that, in addition to the above Salmonella, a region particularly common to transacylases derived from Pseudomonas, Chenohabditis, Haemophilus, and the like is highly conserved. Further, as shown in FIG. 14, even when these two hydropathies were compared, the tendency was very similar.

The oafA encodes a gram-negative outer membrane O antigen sugar acetylase, and this region is considered to be a transmembrane region. Therefore, bcsY appears to be present in the cytoplasmic membrane of acetic acid bacteria. Further, since this gene is interposed between bcsABII-A and bcsCII in close proximity to the C-terminus, which is the hydrophilic region of OafA (see FIG. 15), bcsY is located near the cytoplasmic membrane and bcsABII It is considered to be a gene that synthesizes acylated cellulose in combination with -A and bcsCII.

It has not been known that a transacylase-encoding region is present in the cellulose synthase gene. By using this bcsY gene, it is possible to efficiently produce bacterial acylated cellulose. It is considered possible. Bacterial acylated cellulose has affinity and binding properties for lipids due to its acyl group, and is expected to show different adsorptivity from conventional bacterial cellulose.Therefore, by using it alone or in combination with bacterial cellulose, It is expected to be an excellent polymer material for molecular sieves. In addition, by adding bacterial acylated cellulose to cellulose at various mixing ratios, paper, clothing, food, etc. having different textures such as texture and texture can be obtained. Bacterial cellulose is also expected to be a medical pad or a carrier for biological materials.However, if bacterial acylated cellulose is used in place of this bacterial cellulose, the lipid affinity and bulkiness of the bacterial acylated cellulose will increase. Further, it is expected that the function of holding the biological material can be further improved.

[0035]

Industrial Applicability The novel cellulose synthase gene of the present invention, particularly the acylated cellulose synthesis gene, contributes to efficient production of bacterial cellulose and research for incorporating the cellulose synthase gene into microalgae.

[0036]

[Sequence list]

 SEQ ID NO: 1 Sequence length: 23 Sequence type: Nucleic acid Number of strands: Single stranded Topology: Linear Sequence type: Other nucleic acid Synthetic DNA sequence ATGCARACNC CNCAYCAYTT YTA 23

SEQ ID NO: 2 Sequence length: 23 Sequence type: Number of nucleic acid chains: Single strand Topology: Linear Sequence type: Other nucleic acid Synthetic DNA sequence GCCATNGTNG TYTCRTANAC YTC 23

SEQ ID NO: 3 Sequence length: 26 Sequence type: Number of nucleic acid strands: Single strand Topology: Linear Sequence type: Other nucleic acid Synthetic DNA sequence ATHWSIGCIC AYTAYYTIAT HATGGA 26

SEQ ID NO: 4 Sequence length: 26 Sequence type: Number of nucleic acid strands: Single strand Topology: Linear Sequence type: Other nucleic acid Synthetic DNA sequence CKIGTIACIA CRTARTCICC RAAIGC 26

SEQ ID NO: 5 Sequence length: 641 Sequence type: nucleic acid Number of strands: double-stranded Topology: linear Sequence type: Genomic DNA Origin Organism name: Acetobacter xylin
Linum) strains Name: JCM7664 sequence ATGCAAACGC CGCTACCACT TCTATTCCCC CGATCCGTTC CAGCGTAACC TGGCGGTGGG 60 TTACCGCACC CCGCCCGAAG GCAATCTGTT CTATGGCGTC ATTCAGGATG GTAACGACTT 120 CTGGGATGCG ACCTTCTTCT GCGGATCGTG CGCCATCCTG CGCCGTAAGG CGATTGAGGA 180 AATCGGCGGC TTCGCAACCG AAACCGTGAC AGAGGACGCC CATACCGCGC TGCGTATGCA 240 GCGCAAGGGC TGGTCGACCG CCTACCTGCG CATTCCGCTG GCCAGCGGTC TGGCGACAGA 300 ACGTCTCATT ACGCATATCG GGCAGCGTAT GCGCTGGGCC CGTGGCATGA TCCAGATTTT 360 CCGCGTCGAT AACCCGATGC TTGGCTCGGG TCTGAAGCTT GGGCAGCGTC TTTGCTACCT 420 GTCGGCCATG ACGTCGTTCT TCTTCGCCAT TCCCCGCGTC ATCTTCCTTG CATCCCCGCT 480 GGCCTTCCTG TTCTTCAGCC AGAATATCAT CGCGGCATCT CCGCTGGCAG TGGGGGTCTA 540 CGCCATCCCG CACATGTTCC ATTCCATTGC GACTGCGGCG AAGGTCAACA AGGGCTGGCG 600 GTATTCGTTC TGGAGTGACG GTCTACGAGA CCACCAATGG C 641

SEQ ID NO: 6 Sequence length: 421 Sequence type: nucleic acid Number of strands: double-stranded Topology: linear Sequence type: Genomic DNA Origin Organism name: Acetobacter xylin
Linum) strains Name: JCM7664 sequence ATAACGGCGC ATTACCTGAT AATGGACCAG TAATGACAAC TTTCAACGCA AAACCGGATT 60 TCTCGCTTTT CCTGCAGGCC CTGTCGTGGG AAATCGACGA TCAGGCCGGG ATCGAGGTCA 120 GGAATGACCT GCTGCGCGAG GTCGGCCGCG GCATGGCTGG TCGACTGCAG CCGCCCCTGT 180 GCAACACCAT CCACCAGCTG CAGATCGAAC TGAATGCCCT GCTGGGCATG ATCAACTGGG 240 GATATGTGAA GCTGGAACTG CTGGCGGAAG AACAGGCCAT GCGCATCGTG CATGAAGACC 300 TGCCGCAGGT TGGCAGTGCA GGTGAACCCT CGGGCACCTG GCTGGCCCCG GTGCTGGAAG 360 GGCTGTACGG CCGCTGGATC ACGTCGCAGC CGGGTGCCTT TGGCGACTAT GTCGTCACCC 420 G 421

SEQ ID NO: 7 Sequence length: 12065 Sequence type: nucleic acid Number of strands: double-stranded Topology: linear Sequence type: Genomic DNA Origin Organism name: Acetobacter xylin
linum) Strain name: JCM7664 Sequence characteristics Symbol indicating characteristics: CDS Location: 1..891 Method for determining characteristics: S sequence characteristics Symbol indicating characteristics: CDS Location: 945..1931 Method for determining characteristics : Characteristic of S sequence Characteristic symbol: CDS Location: 2125..4359 Method for determining characteristics: S sequence characteristic Symbol for characteristic: CDS Location: 4364.6775 Characteristic determination method: S sequence Features Symbol indicating the feature: CDS Location: 6781..10755 Method for determining the feature: S sequence feature Symbol indicating the feature: CDS Location: 10758..11225 Method for determining the feature: S sequence ATC GAT ACC GGC AAT AGC GGC GAA TCC CAC AGC GAG GGA CAG GGT TAC 48 GGC ATG CTG TTC GCC GCC GCG GCA GGC GAC CAG ACG GCG TTC GAG GCG 96 ATA TGG GTC TGG GCG CGC AAC AAC CTT CAG CAC AAG ACC GAT GCC CTG 144 TTT TCC TGG CGT TAC CTG GAG GGA CAC AAC CCG CCG GTC GCG GAC AAG 192 AAT AAC GCC ACC GAC GGG GAT CTG CTG ATT GCC CTG GGC CTG GCA CGC 240 GCC GGG AAG CTT TGG AAG CGG GCG GAC TAT ATC CAG GAC GCT ATA AAC 288 ATC TAC GCC GAC GTG CTG AAG CAC ATG ACC ATG AAG GTC GGT CCC TAT 336 ACG GTG CTG CTG CCC GGG GCA GTG GGA TTC GTT ACC AAG GAC GCG GTG 384 ACC CTC AGC TCC TAT TAC GTC ATG CCT TCC CTT CTG CAG GCG TTT 432 GAG CTG TCA GGC GAA TCC CAG TGG CAG ACC GTG ATC GAA AAT GGC CTG 480 CGC ATC ATT GGC AAG GCA CAG TTC GGT GAA TGG AAG CTG CCG CCG TCG CTG CTG ATC AAC CGG CAG ACG GGT AAT TTC TCC ATC GCA AAG GGC 576 TGG CCG CCG CGT TTT TCC TAC GAC GCG ATT CGC GTG CCG CTT TAC CTG 624 TAC TGG GCG CAT ATG CTG TCG CCG GAA CTG CTG GCT GAC TAC TACC GGTC TCTC AAC CAT TTT GGC GCA TCC GCC CTG CCG GGT TGG GTT GAC CTG 720 ACA AAT GGT TCG CGT TCG CCC TAT AAT GCG CCG CCC GGT TAT CTG GCC 768 GTT GCA TCG TGT TCG GGT CTG GCA TCG GCT GGC GAA CTG CCC ACG CTG 816 AAT GCG CCG GAT TAT TAT TCC GCG GCG CTT ACA CTG CTG GTC TAT 864 ATC GCC CGT GCC GAG GGA GGT GGG ATG TGAGTTCAGC CGACAAGGAA 911 GCAGGGACAC CGGCGCCACA CCGTAATGTG GAC ATG GAC AAT CCG CAG GAT GTG CGC ATG CTT ACG ACT GGC TAT GGT CTG AGT GGG GAA GGC TTT CAT 1013 TAC CAT TCC TTC CGG TCC ATC GTG CGG GAC GCG CCC GTG GAT GTC CCC 1061 GAA GAA ACA GAT CAC GAC GAC ACG CAC GCC TAC GCC GAA GAGCAT TAT GCC GAG CCG GAG TCG TAC GAG ACG GCT CCA GCC GCT GCG CCT GCG CCG 1157 GAG CCT GAA CCG CCT GTC GTC ACG CCT GTA GCC ATG CCT CCC ATT GTG 1205 GAG GAA GCA CCG CCA CCG CCA CCG CCA CCG CCA CCG CCCA CCG 1253 CCA CCG CCA CCG CCA CCG CCA CCG GCC CCG GTT GTG CCG GAA GCC GTG 1301 CAT GTG CCG CAG CCG CCT GCG CAG CCC GCG CCG CCT GTC ATG GAA ACG 1349 GTC GCG CCG GAA CCG CCG CCG CCT CCG CCG GAA ACC GTC CCC 1397 GCA CCG CAG CCC AGG CCT GCC GCA ACG ACG CCG GAT GTC GTG CAG TCG 1445 GGC GGG CGC GAA CGG CGT GGC CTG CCG CCG TTT GTT GCG CCT GCT GCG 1493 CCG TCC ACG CCG CCG CGT CCG GCA CCC GCG CCC TCT TTC ACG 1541 GTT GAA GCC CCC GAG CCT GAG GTT GCT GCA ACG GAT GAG TGG GCC CCC 1589 GTG CCC AAG GCG CAG CAG CGC CGT GGA CAG CGC CCG ACC GGG CCT GGA 1637 TTC TTT TTT GCC AAG GCA GGC GAC CGG AC C CAG ATG GCC CGG CTG TTC 1685 CAG CCG ACA CCG GTG CCG ATG CCC CGG CCT GTT TCC AAA CCT GCT TCC 1733 AAG GTG ACC ACG ATG ACC AAG TTC GAC AAG AAT TCA TGG AAT GAA AGC 1781 GCG GGA CGC CGC CCG GCG CCACC GAT AAC TCC CCG ACC CTG ACC GAA 1829 GTG TTC ATG ACA CTG GGG GGG CGG GCG ACC GAC CGT CTG ATC CCC AAG 1877 CCG AGC CTG CGT GAG GCC CTG CTG CGC AAG CGT GAG GAA GAG AAC GAG 1925 CAA TCC GGATTTGGCGC GGATTGGCCGG 1981 ATACAGGGCA GGGGGCCTGG GCCCCGTATG ACCCGCGTGC CATGCGGGCC GAAAGGCGAC 2041 ATGACGGACC AGATGCGTCT GACGGTTTTC TTTTGAACGT AACTATCTGT TTTATCAGTA 2101 TTTATTAATC GGACGAGTTA TTG ATG TCA GAG GTT CAG TCG TCA GCG CCT 2151 GCG GAA AGC TGG TTC GGC CGC TTT TCC AAC AAG ATA CTG TCA CTG CGC 2199 GGT GCC AGC TAT GTC GTT GGG GCG TTG GGG CTT TGC GCC CTG CTT GCC 2247 GCA ACC ATG GTT ACG CTG TCG CTT AAT GAA CAG ATG ATT GTG GCA TTA 2295 GTG TGT GTG GCG GTG TTT TTT ATC GTC GGC CGC CGC AAA AGC CGT CGC T343 ACG CATC CTG GAG GTG TTG TCG GCG CTG GTG TCC CTG CGG TAT 2391 CT G ACG TGG CGG CTG ACG GAA ACG CTG GAC TTT GAT ACC TGG ACG CAG 2439 GGC ATC CTG GGT GTC ACG CTG CTG CTG GCG GAA CTG TAT GCG CTC TAC 2487 ATG CTG TTC CTC AGT TAT TTC CAG ACG ATT TCC CCC CTG CAT CGT 2535 CCG CTG CCG CTG CCG GCC AAT CCC GAT GAG TGG CCC ACG GTT GAT ATT 2583 TTC ATC CCG ACC TAT GAC GAA GCA CTG AGC ATC GTG CGT CTG ACG GTG 2631 CTC GGG GCG CTG GGT ATC GAC TGG CCG CCT GAT ATC GTG AGC TAT 2679 ATT CTG GAT GAC GGC AGG CGT GAG GAA TTC GCC CGT TTT GCC GAG GCC 2727 TGC GGC GCG CGT TAC ATC GCC CGT CCC GAT AAC GCG CAC GCC AAG GCC 2775 GGT AAC CTG AAC TAC GCC ATT AAA CAT ACC ACG GGC GAT ATC CTC 2823 ATC CTG GAC TGT GAC CAT ATC CCG ACG CGT GCT TTC CTG CAG ATC TCC 2871 ATG GGC TGG ATG GTT AGC GAT TCG AAC ATC GCC CTG CTG CAG ACG CCG 2919 CAT CAC TTC TAT TCC CCC GAT CCG CTC CTG CGT GCG GTG GGT 2967 TAC CGC ACC CCG CCC GAA GGC AAT CTG TTC TAT GGC GTC ATT CAG GAT 3015 GGT AAC GAC TTC TGG GAT GCG ACC TTC TTC TGC GGA TCG TGC GCC ATC 3063 CTG CGC CGT AAG GCG ATT GAG GAA ATC GGC GGC TTC GCA ACC GAA ACC 3111 GTG ACA GAG GAC GCC CAT ACC GCG CTG CGT ATG CAG CGC AAG GGC TGG 3159 TCG ACC GCC TAC CTG CGC ATT CCG CTG GCC AGC GGT CTG GCG ACA GAA 3207 CGT CTC ATT ACG CAT ATC G CGT ATG CGC TGG GCC CGT GGC ATG 3255 ATC CAG ATT TTC CGC GTC GAT AAC CCG ATG CTT GGC TCG GGT CTG AAG 3303 CTT GGG CAG CGT CTT TGC TAC CTG TCG GCC ATG ACG TCG TTC TTC TTC 3351 GCC ATT CCC CGC GTC ATC CTT GCA TCC CCG CTG GCC TTC CTG TTC 3399 TTC AGC CAG AAT ATC ATC GCG GCA TCT CCG CTG GCA GTG GGG GTC TAC 3447 GCC ATC CCG CAC ATG TTC CAT TCC ATT GCG ACT GCG GCG AAG GTC AAC 3495 AAG TGC TGG TGG TAT TTC TGG AGT GAA GTG TAC GAA ACC GTC ATG 3543 GCG CTG TTC CTG GTG CGG GTG ACC ATC GTC ACG ATG CTG TTC CCC TCC 3591 AAG GGC AAG TTC AAC GTG ACG GAA AAA GGT GGT GTT CTG GAA CGT GAG 3639 GAA TTC GACTC GCC ACC TAT CCG AAT ATT ATT TTC GCC ATC ATC 3687 ATG GCC CTC GGC CTG TTG CGT GGG CTA TAT GCG CTG ATC TTC CAG CAC 3735 CTG GAC ATC ATT TCG GAA CGT GCG TAC GCG CTG AAC TGC ATC TGG TCG 3783 G TG ATC AGT CTG ATC ATC CTG ATG GCG GTA ATC TCC GTG GGG CGT GAA 3831 ACA AAG CAG CTG CGC CAG AGC CAT CGT ATC GAA GCC CAG ATC CCC GTT 3879 ACG GTT TAT GAT TAC GAT GGC AAT TCG AGC CAC GGC ATTACTA 3927 GTC TCC ATG GGC GGT GTG GCG ATC CAC CTG CCA TGG CGT GAG GTT ACT 3975 CCC GAT CAC CCT GTA CAG GTC GTG ATC CAT GCC GTA CTG GAT GGC GAG 4023 GAG ATG AAC CTT CCG GCC ACC ATG ATC CGC AGT GCC CAG GCG 4071 GTG TTT ACG TGG TCG ATC AGT AAC ATT CAG GTT GAG GCG GCC GTG GTC 4119 CGG TTT GTG TTC GGA CGC GCC GAT GCC TGG CTG CAG TGG AAT AAT TAT 4167 GAG GAT GAC CGG CCG TTA CGA AGC CTG TGG CGT CTC ATC AGC ATC 4215 AAG GCA CTG TTC CGC AGG AAG GGT CAG ATG ATT GCC CAT AGT CGT CCC 4263 AAA AAG AAA CCA ATT GCA CTG CCG GTT GAG CGT AGG GAG CCA ACA ACC 4311 AGC CAG GGT GGT CAG AAA CAG GAA GGA AAG ATC AGT CGT GCG GCC TCG 4359 TGAT ATG AAA ATG GTG TCC CTG ATC GCG CTG CTG GTT TTC GCA ACG GGA 4408 GCG CAG GCT GCC CCG ATT GCG TCC AAA GCG CCA GCC CAC CAG CCT ACG 4456 GGC AGT GAT CTC CCC CCC CTG CCT GC A GCG GCA CCG GTG GCG CCA GCG 4504 GCG CAA CCT TCC GCA CAG GCG GTT GAT CCG GCA TCA GCC GCG CCC GCG 4552 TCC GAT GCG GGA AGC GCC AGC AAT GCG GAT GCG ATA CTG GAC AAT GCC 4600 GAA AAT GCG GCA GTC GTC ACC GAT GTT GCA ACC GTC CAT ACC TAT 4648 TCC CTT CAG GAA CTG GGT GCG CAG AGT GCA TTG ACC ATG CGC GGC GCC 4696 GCC CCG CTG CAG GGG TTG CAG TTC GGG ATT CCG GCA GAC CAG CTG GTG 4744 ACA TCG GTC CGA GTG TCC GGG GCC ATG TCG CCC AAC CTC CAG 4792 CCC GAC AAC AGC GCG GTG ACG ATC ACG CTG AAC GAA CAG TAT ATC GGC 4840 ACG CTG CGT CCC GAC CCG ACG CAT CCG GCG TTC GGG CCG CTG TCA TTT 4888 GAC ATC AAT CC TTC TTT GTC AGC GGC AAC CGC CTG AAC TTC AAC 4936 TTC GCT TCA GGT TCC AAA GGG TGC GCG GAC CCG ACC AAC GGG CTG CAG 4984 TGG GCC AGC GTG TCT GAA CAT TCG CAG CTG CAG ATC ACG ACC ATT CCG C32 CTT CCT CCC CGT CAG CTG GCC CGT CTG CCC CAG CCG TTC TTT GAT 5080 AAG ACT GTA AGG CAG AAA GTC GTC ATT CCG TTC GTC CTT GCA CAG ACA 5128 TTT GAT CCA GAA GTG CTC AAG GCT TCC GGC ATC ATC GCG TCG TGG TTC 5176 GGG CAG CAG ACC GAC TTC CGT GGG GTC AAT TTC CCC GTC TTC TCC ACC 5224 ATT CCC CAG ACC GGC AAT GCC ATT GTG GTC GGC GTG GCG GAT GAA CTG 5272 CCT GCA GCG CTG GGC CGC CCG TCC GTC AGT GGC CCC ACC CTG A 5320 GTC GCC AAC CCA TCC GAT CCC AAC GGC ACG GTG CTG CTG GTG ACG GGG 5368 CGT GAC CGC GAT GAA GTC ATT ACC GCC AGC AAG GGG ATC GGC TTC GGG 5416 TCC AGC GCC CTG CCG GTC GCC AGC CGC ATG GAT GATT GCG CC GAT 5464 GTC GCC CCG CGT CTG GCC AAT GAC GCG CCG TCC TTC ATT CCC ACC AGC 5512 CGC CCG GTG CGG CTG GGT GAA CTG GTG CCG GTC AGC GCC CTG CAG GGC 5560 GAA GGG TAT ACG CCG GGC GTG CTG TCG GTT CCTG TTC TCG CCT 5608 GAC CTC TAT ACG TGG CGT GAC CGT CCG TAC AAG CTG AAC GTG CGT TTC 5656 CGC GCG CCG GAT GGC CCG ATC CTT GAT GTG GCG CGC TCG CAT CTG GAT 5704 GTC GGC ATC AAC AAT ACC TAC TAC CTG CAG TTC TCA CGG GAG CAG 5752 AGC TCG GTT GTC GAT CAG CTG CTG CGT CGT GTT GGC GTG GGC ACC CAG 5800 AAC GCG GGC GTG GAG CAG CAT ACG CTG ACC ATT CCG CCG TGG ATG GTG 5848 TTC GGT CAG GAT CAG CTG CAG TTC TTC AT TTT GAC GCA GCC CCG CTG GCA 5896 CAG CCC GGC TGC CGT CCC GGT CCG AGC CTG ATC CAC ATG TCG GTC GAT 5944 CCG GAT TCG ACC ATT GAC CTG TCC AAT GCC TAT CAC ATC ACG CGC ATG 5992 CCC AAC CTG GCC TAC ATG AGT GCG GGG TAT CCG TTC ACG ACC TAC 6040 GCC GAC CTG TCG CGT TCG GCG GTG GTG CTG CCG GAT CAT CCC AAT GGT 6088 ACG GTG GTC AGC GCG TAT CTC GAC CTC ATG GGC TTC ATG GGG GCG ACG 6136 ACA TGG TAT CCC GTC GGC GTT GAT ATC GTT TCG GCC GAC CAT GTC 6184 AGC GAC GTG GCG GAC CGG AAC CTG ATT GTC CTG TCC ACC CTG TCC AAC 6232 AGT GCG GAT GTA TCT GCC CTG CTG GCC AAC TCG GCA TAC CAG ATT TCG 6280 GAT GGG CGG CGG ATG GGG CTG CGT TCC ACC CTG AGC GGC GTG TGG 6328 AAC ATC TTC CAG GAT CCG ATG TCG GTT ATG AGC AAC ACG CAC CCG ACC 6376 GAG GTC GAA ACC ACG CTG AGC GGT GGC GTC GGC GCG ATG GTG GAG GCG CCG 6424 TGATCGA GCG TCC GGC CGC ACC GTG CTG GCC CTG CTG TCG GGT 6472 GAC GGG CAG GGG CTT GAT AAT CTG GTC CAG ATC CTG GGG CAG CGT AAG 6520 AAC CAG GCG AAA GTA CAG GGT GAC CTT GTG CTG GCG CAT GGT GAC GAC 656 8 CTG ACA TCC TAC CGC AGT TCG CCG CTG TAT ACG GTT GGC ACG GTG CCG 6616 CTG TGG CTG ATT CCC GAC TGG TAT ATG CAT AAC CAT CCC TTC CGC GTG 6664 ATC GTG GTC GGG CTG GTT GGC TGT CTG CTG GTG GTG GTC GTC GTG 6712 CGT GCC CTG TTC CGC CAC GCG ATG TTC CGT CGC CGG CAG TTG CAG GAA 6760 GAA AGG CAG AAA TCG TGATC ATG AAC AGA CGA TAC GTC TTT TCG CTT TCT 6810 GCC GGC CTG CTT GCC AGC AGT TGC ATG GGC GCG ATA A GTG CCG 6858 GTC GCC CGC GCG CAG CAG GCG TCC ACC GCC ATG ACC GGC GCG CAG GCC 6906 ACC GGG GGA ACT GCC GCG CCA CGG CAG ATC CTG TTG CAG CAG GCG CGT 6954 TTC TGG TTG CAG CAG CAG CAG TAT GAC AAT GCC CGG GCA TTG CAG 7002 AAT GCG CAG CGG ATC GCT CCG GAT GCC CCC GAC GTG CTG GAA GTG CAG 7050 GGG GAA TAC CAG ACG GCC ATG GGC AAC CGT GAA GCG GCA GCC GAT ACC 7098 TTG CGC CAC CTG CAG GAA GTG GCA CCTC GGC AGC GCG GCA AAC AGC 7146 CTG AGC GAC CTG CTG CAC GAA CGC TCC ATC TCG ACC GGC GAT CTG TCA 7194 CAT GTG CGT TCG CTT GCG GCA TCC GGT CAT AGC GCG GAA GCC GTT GCC 7242 GGG TAC CAG AAG CTG TTC AAT GG C GGC CGG CCC CCG CAT TCC CTG GCG 7290 ATC GAA TAT TAC CAG ACC ATG GCG GGT GTC CCG GCA GAC TGG GAC CAG 7338 GCG CGT GCG GGC CTT GCG GGT CTT GTG GCG GCC AAT CCG CAG GAC TAC 7386 CGG GCA CAG CTC GCC GCG CAG ACG CTG ACA TAT AAT ACC TCG ACC 7434 CGT ATG GAG GGG CTG GCG CGA CTG AAG GAC CTG CAG GGG TTC CGA ACG 7482 CAG GCC CCC GTC GAG GCC GCC GCC GCC GCC CAG TCC TAT CGG CAG ACA 7530 TTG AGC TGG CTG GTT ACG GCC GAG ACG CAG CCG CTC ATG CAG CAG 7578 TGG CTG ACG GCG CAT CCC GAT GAT ACG GCG CTG AAG GAG CAC ATG CTT 7626 CAT CCG CCG GGT GGT CCG CCG GAC AAG GCC GGG CTG GCG CGT CAG GCA 7674 GGC TTC CTT AAT TCC GGA CGC CTG TCC GCG GCC GAG CAG TCA 7722 TTC CAG TCC GCG CTA CAG ATC AAT TCC CAT GAT GCG GAT TCG CTG GGT 7770 GGG ATG GGG CTG GTC AGC ATG CGT CAG GGT GAT GCG GCT GAA GCG CGT 7818 CGT TGT CAG GAA GCG ATG GCT GCG GAC CCC AAG ACG GCT GAC CGC 7866 TGG CGC CCC GCG CTG GCG GGG ATG GAA ATC AGC GGT GAC TAT GCG GCT 7914 GTC CGC CAG CTG ATC GCA GCC CAT CAG TAC ACC GAA GCC AAG CAG CGC 7962 CTG ACA TCG CTG GCG CGT CAG CCG GGA CAG TTC ACC GGT GCG ACC CTG 8010 ATG CTG GCG GAC CTG CAG CGC ACG ACC GGG CAG ATC GAT GCA TCT GAA 8058 CAG GAA TAT CGG TCC GTT CTG GCG CGA GAT CCC AAC AAC CAG GCC 8106 CTG ATG GGG CTG GCA CGG GTG GAC ATG GCG CAG GGC AAC ACG GCG GAA 8154 GCC CGT CAG CTG CTG TCG CGC GTC GGG CCG CAA TAT GCC ACG GAA GTT 8202 GGC GAG ATC GAG GTG ACG GGC CTG ATG GCC GCG GCG TCG ACG TCG 8250 GAC TCG GCG CGC AAG GTT GCC ATT CTG CGT GAA GCC ATG ACG CAG GCC 8298 CCG CGC GAT CCG TGG GTG CGC ATC AAC CTG GCC AAT GCC CTG CAG CAG 8346 CAG GGT GAC GTG GCG GAA GCC GGG CGG GTC ATG CAG ATC CTG GCC 8394 AAT CCG GTT ACG GCG CAG GAC CGG CAG GCG GGT ATC CTG TAT ACC TAT 8442 GGC GCC GGC AAT GAC GCG GCG ACC CGT CGC CTG CTG TCC GGG CTG TCC 8490 CCC GAG GAT TAT TCG CCG GCC ATC GGT TATTG GAG GAA ATG CAG 8538 ATC AAG GAA GAT CTG GCC AGC CGT CTG TCG ATG GTG CCA AAT CCG GTT 8586 CCC CTG ATC CGT GAA GCG CTT GCC CCG CCT GAC CCG ACC GGC GCG CGC 8634 GGC GTG GCC GTG GCA GAC CTG TCG TC CGC CAG CGT GGT GAC ATG ATC CAT 8682 GCC CGC ATG GCC CTG CGT ATC GCC TCG ACC CGT ACG ATT GAC CTG TCG 8730 CCG GAC CAG CGA CTG GCC TAC GCC ACC GAA TAC ATG AAG ATC AGC AAC 8778 CCG GTT GCC GCC GCC CTG CTT GCG CCG CTG GGC GAT GGT AGC GGA 8826 AGC GGA GCA GGC AAT GCG CTG CTG CCG GAA CAG CAG CAG ACG CTC CAG 8874 CAG CTG CGC ATG GGC ATT GCC GTG GCG CAG TCC GAC CTG CTG AAC CAG 8922 CGT GGC GAT CAG CAG GCG TAT GAT CAC CTG GCC CCG GCG CTG CGG 8970 GCG GAT CCG GAG GCG ACA TCG CCC AAA CTG GCG CTG GCC CGG CTG TAT 9018 AAC GGC GAA GGC AAA TCC AGC AAG GCA CTG GAC ATC GAC CTG GCG GTA 9066 CTG CGC CAT AAC CCG CAG GAT CTT GAT GCC CCC CAG GCC GCC GTG CAG 9114 GCC GCG GTC AAT AGT GGC CGC AAG AGC CTG GCC ACC CAT CTG GCG ATG 9162 GAC GGC GTA CAG GAA AGC CCG ATG GAT GCC CGC GCA TGG CTG GGC ATG 9210 GCC GTG GAT CAG GCA GAT GGA CAT GGG CAT CGG ACC ATT GCC GAC 9258 CTG CGC CGG GCG TAT GAC CTG CGC CTG CAG CAG GTC GAG GGC TCC CGC 9306 TCT GCC TCC GGC CCG GCA GCG ACC GAG GAA GAT GCG CTG GCG CCG CCT 9354 TCC AGC AAC CCG TTC CGC CAC CAT GGC TAC GGG CGG CAG ACG GAA CTT 9402 GGC GCG CCG GTC ACG GGT GGG TCC TAC AGC ATG GAA GCG ACC TCA CCC 9450 GAG GCT GCG GAC CAG ATG CTG TCT TCC ATT TCC GGG CATC ACC 9498 CTG CGT GAA AAT CTT GCC CCG TCC ATT GAC GGT GGT CTC GGG TTC CGG 9546 TCG CGT TCG GGT GAA CAC GGC ATG GGC CGC CTG ACG GAA GCC AAC ATT 9594 CCC ATC GTC GGG CGC CTG CCG CTG CAG GCT GGT GATC CTG ACC 9642 TTC TCG ATC ACG CCA ACC ATG ATC TGG TCG GGT GAC CTG AAT GCC GGT 9690 TCG GTT TAT GAC GTG CCC CGT TAC GGC ACC AAC ATG GCG ACG GAG GCG 9738 TAC AAC CAG TAC GTC AAT TCC TTG AGC CAGC AAC AAC AGC AGC AGC 9786 CTG CGG ACC CAG CAG ATT CAG GGC GGG CAG GGT GAA GCG GGC TTT GCG 9834 CCG GAT GTG CAG TTC AGC AAC AGC TGG GTG CGG GCG GAT GTC GGC GCA 9882 TCG CCG ATC GGC TTC CCC ATC GACC GTG CTG GGG GTC GAA TTC 9930 TCG CCA CGT GTC GGG CCG GTG ACG TTC CGT GTC AGT GCC GAG CGC CGT 9978 TCG ATC ACC AAC AGC GTC CTG TCC TAT GGC GGC CTG CGT GAT CCG AAC 10026 TAC AAT TCG GCC CTG GGC CGT TAC GCG CTG AAC CAT TAT GGC AGC CAG 10074 CTT GCG TCG CAG TGG GGG CAG GAA TGG GGT GGT GTC GTG ACC AAC CAC 10122 TTC CAT GGC CAG GTC GAG GCG ACG CTG GGC AAT ACC ATT CTG TAT GGT 10170 GGC GGT GGC TAT GCG CAG ACC GGT AAG AAC ACG CGG AGT AAT AAC 10218 GAG CGT GAG GCC GGT ATC GGT GCC AAT ACA CTG GTG TGG CAC AAC GCC 10266 AAC ATG CTG GTG CGG ATC GGT GTC AGC CTG ACC TAT TTC GGT TAT GCC 10314 AAC ATC GAC TAT ACT TAT GGG CAG GGT GGC TAC TTC TCG CCC 10362 CAG TCC TAT TAC TCA GCT ACC GTT CCG ATC CGT TAC GCC GGG CAG CAC 10410 AAG CGA CTG GAC TGG GAT GTG ACC GGC AGC GTG GGC TAT CAG GTG TTC 10458 CAC GAA TCC CCA TTC TTC CCG ACA TCC TCG CTC TTG CAG TCG 10506 GGG GCC CAG TAC ATT GCC GAC TCG TAT ATG CAG AAT GCG ACG GCC TCG 10554 GAT TAT CTG TCA GAG GAA ACG GTA GAC AGG GCC TAT TAT CCC GGG GAT GCT 10602 ATC AGT CTT ACG GGC GGC TTC AAT GCT AGG GTA GGG TAT CGA 10650 TTT ACA CAC AAT CTT CGT CTT GAT CTG TCG GGG CGC TGG CAG AAG GCC 10698 GGT AAC TGG ACT GAA AGC GGC GCC ATG ATT TCC GTA CAC TAT CTT ATT 10746 ATG GAC CAG TA ATG ACA ACT TTC AAC GCA AAA CCG GAT TTC TCG CTT 10793 TTC CTG CAG GCC CTG TCG TGG GAA ATC GAC GAT CAG GCC GGG ATC GAG 10841 GTC AGG AAT GAC CTG CTG CGC GAG GTC GGC GGC ATG GCT GGT CGA 10889 CTG CAG CCG CCC CTG TGC AAC ACC ATC CAC CAG CTG CAG ATC GAA CTG 10937 AAT GCC CTG CTG GGC ATG ATC AAC TGG GGA TAT GTG AAG CTG GAA CTG 10985 CTG GCG GAA GAA CAG GCC ATG CTC AGC CAT GAA GAC CTG CCG CAG 11033 GTT GGC AGT GCA GGT GAA CCC TCG GGC ACC TGG CTG GCC CCG GTG CTG 11081 GAA GGG CTG TAC GGC CGC TGG ATC ACG TCG CAG CCG GGT GCG TTT GGC 11129 GAT TAT GTC GTC ACC CGT GAT GTG GCG GAA GAC CTG AAT TCC GTC 11177 CCG ACC CAG ACC ATC ATT CTG TAC ATG CGT ACC CGC AGC AAC AGC AAC 11225 TGACCGGACC TGTCCGGGCC GCGCTGTTGC CGCATGACCC CGCCCACAGG CATGTATATG 11285 GCCTGCCGGC GGGGTTTTTT TACACCTGCT GGCCCAATTT CTCGTCCTGC CTTGCGCTAG 11345 ATCATGCGGA TTGCAGGACG GGTGTTTCAG TAATTATGAC CACAGCGGGC GGGACCGTCC 11405 GGCTCGTATC CCTGCTGTCC TGACCATTAT GATGAGGAAG CCGCCGC ATG AAG CT G 11461 TCC CGC AAG ATA TTC CTG CTG TCC GCC GTG GCG TGC GGC ATG ATG GTG 11509 GCG CAT GAC GCC GCC CAT GCA GCG CAT CAT GCC CCG GGT GAT CCG GCC 11557 GAT GAA AAG GCA CGT CAG GTG CTG GCG CAT ATG AGC CGA GAC AAG 11605 ATG TCC CTG CTG TTC AGT GTT GAC GGT GGT GGG TTC AAT GGC AGC GTC 11653 GCC CCG CCG GGC GGC CTT GGT TCG GCG GCG TAT CTG CGT GCG CCT GCG 11701 GGG TCG GGC CTG CCG GAC CTG CAG ATT TGG GAT G CTT GGC ATC 11749 CGC AAC CCG GCC CAT ATC CGT AAG GAC GGG GCG GCG GTT TCC CTG CCA 11797 TCA GGG CTG TCC ACC GCC AGT TCG TGG GAC ATG GAC ATG GCG CGT GAG 11845 GCC GGG GCC ATG ATC GGC CGC GAA GCG TGG CAG GGC TTC AAT GTC 11893 CTG CTG GGC GGC GGG GCC GAC CTG ACC CGT GAC CCG CGC GGT GGC CGC 11941 AAT TTC GAA TAT GCA GGC GAG GAT CCG CTG CAG ACC GGG CGC ATG GTC 11989 GGC AGC ACC ATT GCG GGC GTG CAG TCA GTG ATT TCC ACC CTC 12037 AAG CAT TAC GCG ATG AAC GAT CTC GAG G 12065

SEQ ID NO: 8 Sequence length: 35 Sequence type: number of nucleic acid chains: single-stranded Topology: linear Sequence type: other nucleic acid Synthetic DNA sequence CTGATGCAGA CCCCGCATCA CTTCTATTCC CCCGA 35

SEQ ID NO: 9 Sequence length: 35 Sequence type: number of nucleic acid chains: single-stranded Topology: linear Sequence type: other nucleic acid Synthetic DNA sequence ACGCGCACCA GGAACAGCGC CATCGTGGTT TCATA 35

SEQ ID NO: 10 Sequence length: 446 Sequence type: nucleic acid Number of strands: double-stranded Topology: linear Sequence type: Genomic DNA Origin Organism name: Acetobacter xylinum
Linum) strains Name: JCM7664 sequence CTCGAGACCT CGCGCGTGGA TGTCAGCCTC AACAACAACT ACCTGCAGAG CTATACGCTC 60 TCGCCGCCGG GGCTGTGGCG CAAATGGTCC GAACGCCTGG TCAACCAGCA CGCGGGCGCG 120 GTGGGGCATG TCACGGCGCT GCCGCCATGG CTTCTGTTCG GGCAGAACCA GCTGCAGTTC 180 AACTTTGACG CCCGTCCCAT CGACCGGGGC GCGTGCCGCC GCACGCCGGG CGACATCCAC 240 ATGAGCGTGG ATTCGGATTC CACGCTGGAT TTCCGTCGCG GCTACCACTT CGCCGAGATG 300 CCCAACCTGT CCTATTTCGC CGAGGCCGCC TTCCCGTTCT CGCGCATGGC CGACCTGTCG 360 GAGACGACCG TCGTCCTGCC CGACCACCCC GACACGGGCA CGACGGGGGC GTTCCTCGAC 420 CTCATGGGCT TCTTTGGCGC GTCGAC 446

SEQ ID NO: 11 Sequence length: 17091 Sequence type: nucleic acid Number of strands: double-stranded Topology: linear Sequence type: Genomic DNA Origin Organism name: Acetobacter xylin
linum) Strain name: JCM7664 Sequence characteristics Symbol indicating characteristics: CDS Location: 3745..8298 Method for determining characteristics: S sequence characteristics Symbol indicating characteristics: CDS Location: 8334..9119 Method for determining characteristics : Characteristic of S-sequence Characteristic symbol: CDS Location: 9207..10364 Method for determining characteristics: S-sequence Characteristic symbol: CDS Location: 10375..14295 Characteristic determination method: S-sequence symbols characteristic features: CDS existing position: 14332..16038 method to determine the characteristics: S sequence GGATCCAGCC CAGAATGATG AAACCCGCGC CGCTGCCCAT CGCCAGGGCC GTTTTCGTGA 60 TGAGTGTATT AAGCGCGTAA AGGGTGCTGG ACTTGCTGGT CCCTGTTTTC CAGATGTCAT 120 AATCTATGAC ATCCGCCAGT ATGGCATATG GCATGAAATT GGCGGGGGCG GATAACGCGC 180 CGCCCGCCGC TGTCAGCAGC ATGATGAAGG GCAGGCTGTG CATGCCCGGC GCAGGCAGCA 240 GGCCAAGCGG CCGTGTCAGT GCCGACAGGA TCATGCTGAT GGCCCACAGC GCGTGACGTT 300 CCATCCGCCG TTCTGCCCGA CCCCATAACG GAATGGCCAC GATGGTGGAC AGGAAAAACA 360 CGATCATGAT GAGCGGGAAC ACGCTGCCCA CGCGCAGGTA GTCATTCAGG AAAAGAAACG 420 TCGTGGAAAA CCATATGCCC TGTCCCACGC CCCACAGGGC GATGGCGAGG AAATACAGGG 480 CAAGGGGACG GTTCTGTTTC AGGGATCGCG CCAGGTCACG CAGGGTCGGG GCAATGGTGC 540 GCGCGGATGG CCTGTCGGGA ACATATCGCA TGATGCATAT GACAGCGGGC AGCATCAGTA 600 CTGCATACAG CCAGACAATG ACGCGCAGCG TTCCCTGCGT GAAACCCGTC GTGCCGTAAA 660 ACCGGAACAG GATGACAGGC ACCAGCCAGA AGATCAGGCT GCCCGCAACG GTAAACAGGC 720 CGATTGCTGC ATTGATGCGC GTTCGCAAAG GCGGGGTTGA CCCGAGTTCC GCGCTCCATG 780 CGCTACGGGG GATTTCAAAC AGGGAATAGC CAAAATACAG GACAAACGCC CAGACCCCAT 840 AGTACAGGAT GGTCGAATGT GACGAAGGCT GGAACAGGAA AAACAGGGCA ACGCAGCAGA 900 TGACGCCACT GGCGATCCCC CACGTCCCCC GTCCATTGAA ACGGGTGCGG GAACGGTCGG 960 ACAGAAAACC GATGGCCGGG TCCAGCAGGG CATCAATGAC CCGGCATAAT GATGTGGCTG 1020 TCCCGATCTG CAGCAGGGTC ACGGCCGTAT GGCTGGCGTA AAAGGCCGGG ATGAAAATAT 1080 TCATCGGCAG GATGATGAAA GCGGCAGGGA GTGCAGGAAA GGCAAAGAGC CAGGTTTGCA 1140 GTCTGGTCAG TCCCGTTTGT GCAATCGTTT GCTTCTGCAC GGAGGGGGAA GGGGAAGGAG 1200 CCGGAACCAT GACAGAAAAA TTCTTTCCTT CTCTCCACGA ACCCG CCCTT GATAAGCAAA 1260 AAACAGCTTG CATGAAAGGC CGGAATGAAA ATAGTCATGA CTGGACAGTT AAAGAGATGA 1320 ACGGCACGGG GCGGGAGAAT GGAAGGGCGT GTGTTGCCCT GTTCGCGCGG TATGTGCAAG 1380 CGCATGTCGG CAGGGAAGAG GTGAAATCCG AAGCCATGAT GCGGCAGTCC CGGCACATGC 1440 TTCATGGTTC TCATGTGAAG GCAGCAGGGA ACGGCTTTGC CCCCGTGGCG AAAAGGGTCG 1500 CCGGGGATCA GGGCTTGGCC CCGAGCACCT TTGGCAGGTC CCCCCTTATG AAGGCATCAA 1560 GAATGCCAGT GAGGAAGGTC GCGATGCTTT CGCGCGGATT GTCCGTTTTG GAAAACGGGC 1620 CTTCGACGGG ATTGGCGATG AACAGGTTGG AAAGGCCGCA GAGCATGGCG TACATCCCGT 1680 ATCCGATCTG CACCTTGCTT CCCAGTGTTG CCATGTCCCC GGGACCAAAA TCATCACGGC 1740 TGGTGAAGAC CCTGTAGCCG TACATCAGTT CCATGAGGTC CTCGAACGCC GCACTCGCGG 1800 TCAGTCGGTA ACGGGGGTAG GTATAGGCAT CGGGTATGTA GCCGAACATC AACCGATAAA 1860 GCTGCGGGCG GAGCCGTGCG AAGTCCACGA AATACAGGAC CGTCCGCTTC AGCGCTTCGG 1920 TCCGGGTTGC CGAGGTGCGG CTGATATGGG CGGCCTGTTC GCGGAAAACC TCGAAATAAT 1980 CTGCGGCAAT TTCCAGCAGC AGGTCGTTCA GGCTTCCGTA ATGATTATAG AAATTTGCAG 2040 GCGCAACCTT CACCTCCCGC GCCAGGCGAC GTACTGACAG ATCTTCCAGC CGTTCGGTGT 2100 CCAGTATCCT GACGGTTGCA ATCCGCAGGT CCTCGGCAAC ATTTCCTTTA TGGTAGGCTC 2160 TTGAAATGGG ACGTCATCCT TATGGGCATG GCGGGGATCG GGTAGGGAAA CATATGGTGA 2220 AAGATGGCGG GGGAACCATC CTTATGTTGG AAATGTGATC CCTGACGATT ATGACCTTAA 2280 ATAACGGCGA CAGAAAGATG GTTTCATATA CATGATCTGA CGTAAGATTC CTGTGCTCCC 2340 TGCCTCTCCC CCACTGCATT ATGGTAGAAA CCCTTAACCT GCATTTTAAA GTCCAGGATT 2400 TCCCTGCAAT CAAGGGGGTA GGGGCATCTG CCCCTGCGCC GGGTCATCTG CCGGCCGGGT 2460 CAGGGCATGG TACAGTTCAT TGTGAATGAA GGCATCCACT ATGCCTTCGA TCATCTGCTG 2520 CGGGCTCTGT CCCGGTTTGT TGTCAAACGG CAGCTGGCCG TCGGCCAGCA TCATCGCCAG 2580 TCCATGCCCC ATGGCGCACA GGCCATATCC GATACGGGCC CGTTTCCTGG TGCCTTCAAT 2640 ATCATGAAGG TGGAGCATGT TCCGGCCGTA TATGATCTCC AGCAGGGCGG CAAAGGCCTG 2700 CATTGCCGCG GCGTGATAGA TCCGGTGTGA AATGGCGTTG GGTACATAGC CGAACATGAT 2760 GCGGAATGTC TGCGGATGAC GCTGCGCGAA GCCTGTATAT TCCACCAGTG ACGCCTTCAG 2820 CGCATCCGTG CGTGTTGTTG CGTTGCGGTG GAAATAGCGG AGCTGCCGGG TGCATTCCCT 2880 GAATGCCTCG GCGGCAATCG TCAGCAGCAG GTCATTGATG CTGGAAAAAT AATTGT AGAA 2940 ATTTGCTGGC GTCACGCCGA CTTCCCGCGT CAGGCGGCGC ACGGACACGG ATTCGACCGT 3000 TTCCCTGTCC AGCAGGTGTC GGGCCATGCG TATCAGGTCT TCTGCAACAT GGCCCTTGTG 3060 ATAGGGGCCA TTTTTTGTTG TGGGCGAAGG GGCGTTCATC CGTTTCTTTC TTCCCTCCAT 3120 GCCCCGCGCA GGGGAATGGA AATACCGTAC TACCGTTACC GGGCGGACAT GCGCGGGCGT 3180 TGCGCAGGCA TAATGAACGT CGTTCATATA AAAATGCGAC AAAAACCGTC AAGCAGAACG 3240 GATGCACGCA GGGCATGATC CAGATGGGTG GATAATATGC AGTCATGCCT GATTCGGTAC 3300 AATCAGGTTG TGCAAATATA ACGCTGATAT TCAAACAGAA ATTTAATGGA AATATTGTGT 3360 CAGACATGCC GGGTGGTCAG GGATCATCCA CTTTCCGGGT CATGTCTGGT TGTCCTGGCC 3420 TGTCTATGGG AAATGGAAAA ACCTACCCGG AATGATGGGT ACGCCTTGAC ACATGCTTAA 3480 TGTAAAATTA ACACCGTTCG CATTGGCCTG ACGCGACATC ATGTTGGGCA AGCGGTTGCA 3540 AATAAACATG TGTCGATATT TTTCAGGATA TGTCCAGGGT ATCAGGCATT AATTCCTAAG 3600 GTTTGAACTA AACAGTGTTT ATTTCCGGGG CGGTCATTCG CTTTTCCGGA ACATGCGTGG 3660 GTACCCGTCC ATCCGCCATG GCCACACGGC CGTGGACGGG CATGGGGCGG GTATCCGTAG 3720 TGTAGGGTAG CGGCATAGCT GACG ATG TAC GGA ACG TGG TTC ACG ACG GGG 3771 AAG GTC ACC GAC CTG CTG GCG CGC ACG GGG CTT GAC CGT GTG CCG GTC 3819 TGG GTG CCG GTG GTG CTT GGC GTT GTC CTC ATG GCC TTC GTG GGG TCG 3867 GTC AGG ATC GAT CCC GCG CTG CAG GGG TGG GTC TCG GTC GCG AGC 3915 ACG CTG CTG CTG GTG CTC AAC CGG CGG CGC GGG CGG GGG ATC ACC GTG 3963 TTC CTC ATG ATG CTG TCG CTG CTG GTG TCG CTG CGC TAC ATC GTG TGG 4011 CGG CTG ACC GCG ACG GTG CAG TTC AGC AAC TGG CTG CAG ACG CTG 4059 GCC GTG CTG CTG CTG CTG GCC GAG GCC TAT GCG CTG ATG ACG CTG TGC 4107 CTG AGC TAT TTC CAG ATG GCC TGG CCG CTG CGG CGG CGC AGG GAA CAC CCC 4155 CTG CCC GAG GAC ATG GCG CAG TGG CCG AGC GTC GTG GTC CCG 4203 TCC TAT AAC GAG GAA CTC TCC CTC GTC CGT TCG ACG GTG CTC GGC GCG 4251 CTG GAC CTT GAC TGG CCC GCG GAC AGG CTG AAC GTC TAC ATC CTT GAT 4299 GAC GGG CGG CGG AAG GCC TTC CAC GAC TTC GCG GCG GGG GCG 4347 GGC TAC ATC ATC CGC GCC GAG AAC AAC CAC GCC AAG GCC GGC AAC CTC 4395 AAC CAT GCG CTG GCG GTG ACG GAC AGC CCG TTC GCG GTG ATC TTC GAC 4443 TGC GAC CAC GTG CCC ACG CGC GGG T TC CTC AGG CGC ACC ATC GGC TGG 4491 ATG ATG GCC GAC CCC AAT CTT GCA CTG CTG CAG ACG CCG CAC CAT TTC 4539 TAC GCC CCC GAC CCG TTC CAG CGG AAC CTC GCC GGC GGG ATG CAC GTC 4587 CCG CCC GAA GGC ATC ATG TAT GGC CTG GTG CAG GAC GGC AAC GAC 4635 TTC TGG GAC GCG ACG TTC TTC TGC GGC TCC TGC GCC ATC ATC CGG CGC 4683 GAG GCG GTG ATG GGC ATC GGC GGC TTC GCC ACC GAG ACC GTG ACC GAG 4731 GAC GCG CAT CGCG AAG ATG CAG CGC CGG GGC TGG GGG ACG GCC 4779 TAC CTG CGC GAA CCG CTT GCC GCC GGC CTT GCG ACC GAA CGG CTG ATC 4827 CTG CAC ATC GGC CAG CGC GTG CGC TGG GCG CGT GGC ATG ATC CAG ATC 4875 ATG CGG CTG GAC CCC ATG CTG GGG GCG GGG CTG CGG TGG GAA CAG 4923 CGG CTT TGC TAC CTG TCG GCC ATG TCG CAC TTC CTG TTC GCC ATC CCG 4971 CGG CTG ACC TTC CTG GTC TCG CCG CTG GCC TTC CTG TTC CTG GGC CAG ATC ATC ATC GCC TCG CCA CTG GCC ATC AGC GTC TAT GCG CTG CCG 5067 CAC ATC TTC CAT TCG GTC ATT ACC CTG TCA CGC ATC GAG GGG CGG TGG 5115 CGC TAT TCC TTC TGG AGC GAG ATC TAC GAG ACC TCG CTG GCG CTG TTC 516 3 CTC GTG CGC ATC ACC ATC GTC ACC CTG CTG CAG CCG CAC AAG GGC AAG 5211 TTC AAC GTG ACG GAC AAG GGC GGG CTG CTG GCG CGG GGG TAT TTC GAC 5259 TGG GAC GCG GTC TAT CCC AAC GTC ATC CTC GCC GGG GTG CTG GCG 5307 GCC CTG CTG CGC GGG GTG TTC GGC ATC GTG TGG CAG TTC CAT GAC CGT 5355 CTG GCG CTG CAG TCC TTC ATC CTG AAC ACG CTG TGG GTG GTG ATC AGC 5403 CTG ATC ATC GTG CTG GCG TCG ATC GCG GTG AGG CGC GAG CGG CAG 5451 ACG CGC AAC GCG CCG CGC GTG AGC GTG CGG CTG CCC GTG GTG GTG ACC 5499 GAC GCC CAT GGC CGG CAG ATG GAG GGG CAT ACG CAC GAC ATC TCG CTC 5547 GGC GGC CTT GCG GTG GGC ACG CGG CTT GCG ACG CC ATG GTG GGG 5595 GGC GAG GTC ACC GTG CGG TAC GAC AGC GCG CGC GAC GGC ATC CAT GTC 5643 GGT GTG CCC GCG CGG GTG CTG GAC GCG CGC GAC GGC ACG CTG CGG CTG 5691 CGG TGG GCC GTG CGC GAC CTG GAG CAGAGCAG GTG GTC AGC ATG 5739 GTC TTC GGC CGC AAC GAC GCC TGG GCC GGC TGG GCG GAT TTC GCG CCC 5787 GAC CGC CCG CTG CGC AGC CTG GCG ATG GTG TTC CGC AGC ATA GGC GGG 5835 CTG CTG CGG CGG CGC CCG GCG GAG GCG CCG CGC GCA CTG CAT GAA ATG 5883 GGG GAA GGC GAG CTT CCC GCC ACG GAA GAG AAA CTG GAG AAA CAA AGC 5931 TTC GTG CTG AAA CCT GTC CCC CGT AGC GCG CGT CAT GGC GCT ACC GCA 5979 TCC GCC GCC CTG TTT GTC GCG TTT ACG GCC CTG GTC CCG GCG GCC ATG 6027 GCG CAG GAG GCG CCG TCC CCG GAC CAG TCG GGG GTC ACG GCG GAA ACG 6075 CCG TTC GGG GAC AGC AAT ACC GGG GTT GTC CCC GAC GCG CTG CCG GCC 6123 ATC GTC CCCC GCC GCC GAC CGG ATC AGC GAT GCC GAG GTG ACG CGC 6171 ACG CTC ACG TTC CGC AAC CTT GGC GCG ACG ACG GGG CCG CTG ACG CTG 6219 CGC GGC TAT TCC CCG CTG CAG GGG CTG GAT GTG GTG GTG CCG GCC AAC 6267 CGG GTG GTG ACG GCG CAG CTG ACG CTG TCG GGC GCG CTG TCG CCC 6315 TCG CTG CTG CCT GAG GCG AGT GCG GTG ACC GTC ACG CTG AAC GAA CAG 6363 TAT GTG GGC ACG CTG AAG GTG GAC CCG CAG CAC CCG CAG TTC GGG CCGTTG AGC TGT ATC GAC CCG CTG TAT TTC ACG GGC GAC AAC AAG CTC 6459 AAT TTC CAC TTC GCC GGG GAA TAC CGC CGC GAC TGC AAC GAC CTG TTC 6507 AAC GAG ATC CTG TGG GCG CGC ATC TCG GAC ATG TCG CGC ATC ACG CTG 65 55 ACG ACG GTG CGC ATC ACG CCC GAA CGC AAG CTC TCG CGC CTG CCC GCG 6603 CCC TTC TTC GAT CCC AAC CAG CGC TCC ACC CTG CGC GTG CCG GTC GTG 6651 CTG CCG GCG ACG GGG GAC AGG GGG GCA CTC CGG GCG GGG CGG GTG 6699 GCG TCG TGG TTT GGC CGC ATC GCC GAT TTC CGC AAG CTG TCC TTC CCG 6747 GTC TCG ACC ACC ATC CCC GCC TCG GGC AAC GCG GTG GAG GTG GGG GTG 6795 AAC CTG CCG GTT GAC GCC GAA GGC GGC AGG CCC GCC GGG ATG CTG 6843 GCC GAA GTC GCC AAC CCC AAC GAC CGC TGG GGC ACG GTG CTG GTG GTG 6891 ACG GGC CGC ACC GCG CAG GAG GTC GAG GTC GCC GCG CGC GCG CTG GTG 6939 TTC TCG CCC GAT ACG CTG GGC GGG GTG GCG TAG A GTC AGC GAT 6987 GTC AGC CTC GAG ACG CGG CAC CCC TAT GAC GCG CCG GCC TTC GTG CCG 7035 ACG GAC CGC CCG GTG CGC TTT GGC GAA CTG GTG GGG GCG GCG GAC CTG 7083 CAG GGC GGC GGC TTC GCG CCC GCG CGC ACG CCG TTC CAC CTG 7131 CCG CCG GAC CTG TAT ACG TGG CGC GGG CGG CCG TTC CTC ATG AAC ATG 7179 TGG GTC CGC GCT CCC GGT GGC CCG GTG GTG GAC CTC GAG ACC TCG CGC 7227 GTG GAT GTC AGC CTC AAC AAC AAC TAC CTG CAG AGC TAT ACG CTC TCG 7275 CCG CCG GGG CTG TGG CGC AAA TGG TCC GAA CGC CTG GTC AAC CAG CAC 7323 GCG GGC GCG GTG GGG CAT GTC ACG GCG CTG CCG CCA TGG CTT CTG TTC 7371 GGG CAG AAC CATCTCG AAC TTT GAC GCC CGT CCC ATC GAC CGG 7419 GGC GCG TGC CGC CGC ACG CCG GGC GAC ATC CAC ATG AGC GTG GAT TCG 7467 GAT TCC ACG CTG GAT TTC CGT CGC GGC TAC CAC TTC GCC GAG ATG CCC 7515 AAC CTG TCC TTC GAG GCC GCC TTC CCG TTC TCG CGC ATG GCC 7563 GAC CTG TCG GAG ACG ACC GTC GTC CTG CCC GAC CAC CCC GAC ACG GGC 7611 ACG ACG GGG GCG TTC CTC GAC CTC ATG GGC TTC TTT GGC GCG TCG ACA 7659 TGG TAT CCGCG GGC GTG ACG GTC ATG GGC GCG GAC GAG GTG GCG 7707 CAC ACC CCG CCG AAG GGC GAC ATC GTC GTG CTG GGC ACG GCG GCG CAG 7755 CTG GGG GGG GCG GCG TCG GGC CTG CTG GCG CGC TCG CCC TAC GTG ATC 7803 CAC GAC CGG ATC ACG GTG GGC CAG CGC ATG GGG CTG CAG GGG ATC 7851 TGG TAC CTG TTC CAG GAC CAC GAC CAC GCC GGG CTG AAG GAC GGG GTG 7899 ACG GCC AAC CTC AAC GCG CCG ATC GCC GAG GCG GGA GTA CTG CTG GCC 7 947 GCG CAG TCG CCC TAT GAC AGC CAG CGC TCG GTC GTG GCG TTC ACG GGC 7995 GAT ACG CCC GAA CGC ATC CAT GAC CTG GTG CTC AGC CTG CGC AAC AAG 8043 GGC GAC CTG CCG TCG CTG CAG GGG GAC CTG GTG CTC AAG GAC 8091 CGG TTC ACC AGC TAC CGC ACG GCG CCG GTC TAT ACC GTG GGC TCC CTG 8139 CCG CTG TGG CTG CGC CTT GAC TGG TTC CTG GGC CAC CAT CCC TCG GCC 8187 CTT TAC CTG GCG GGG CTG GCC GGG GCG GGG CTG GCG GCA GGG GTC 8235 TGG GCG TGG CTG CGT GGC TGG TCG CGC AAG CGG ATC GCG CGC GAC GAT 8283 CTG ACG GGG GAG TTG TGATAGGTCA TATACACCTC CCTTGCGTAT CTGCG ATG 8336 CCC GCT GGC GTG GCG GCG TGG CGG CGT GAC CGC CGG CACG CCT GCC CGG TGC GCG GAC ACG GCA CTT TTC CGA ACC AAT CCC 8432 CAA CTG GAG AAA TAC GAA ATG AAT GCC CTT CTT GCA GGC CTG ACC CTG 8480 CTG ATC ATT GGC GAC AGC CAC GTC ACC TTC AAG GAC TCC CTG CTC TCG 8528 CTG CCC GAT GAG TTC ACA AAG CAG GGC GCG AAG GTC GTG ACG TAT 8576 GGC GTG TGC TCG TCC ACC GCC GCC GAC TGG GTG GTG CCC AAC CCC AAC 8624 AAT GGC TGT GGC GCT GCC GAA CGC GTG GGG GAT GCG CCG ATC GGC GCA 8672 CCC GAC ATG AAG CCG GCC TCC CCG CCG CCG ATC ACC TCG CTG ATC GAG 8720 AAG TGG CAT CCC AAC GTG GTG ATG GTC ATC CTG GGC GAC ACG ATG GCG 8768 GCG TAT GGC CATG ACC TCG AAG GAC TGG GTG GAT GAA CAG GTC 8816 AAG ACG CTG ACC TAC GCC ATC GGC AAG ACG GCG TGC ATC TGG GTC GGG 8864 CCG ACC TGG GGG CAG TTC AGC CCG CGC TAT GGC AAG ACC GAC CAG CGC 8912 GCG ACG AGC ACG TTC CTC AAG GGT GAG GTC GCG CCG TGC AGC 8960 TAT GTC GAC GGG ACG GCG CTG CTC AAG CAG GGT AGC GTA AAC ACC ATC 9008 GAC GGC ATC CAC GCC ACC CCC GAG AGC TAC AGG GTC TGG GGC GAT GCG 9056 ATC GTG CAGCG CTG CCC GAA CTG GAG AAG CTG AAG GAC GCG CCG 9104 CCC GCG CCC GCG CAG TAAGCCCGTC CTGACGCGGC GGGCGGCCCG GAACACCGGG 9159 CGCCCGCCGC CCTGCCCGAT ACTGGCGCAC GCCATGGAAA CCCACTG ATG CTG CAA CCC CCG CACC CCG CACC CCG CACC CCG CACC CCG CACC CGA CCG CACC CGA CCG CACC CGA CCG CACC CGA CCG CACC CGA CCG CACC CGA CCG CACC CGA CCG CACC CGA CGA CCG CACC CGA CCG CACC CGA CGA CCG CACC CGA CGA CCG CGA CGA CCG CACC CGA CGA CCG CACC CGA CGA C Ast TTC TTC CCC AAG AAC CGC CGG CGC GAC ATT GAC GGG CTG CGT 9311 GGC CTG GCC ATC GCG CTC GTG GTG CTG TTC CAT GCG GGG TGG CTG AAG 9 359 GGC GGG TTC ATC GGC GTC GAT GTC TTC GTG GTG ATC TCG GGG TAT TTC 9407 ATG GGG CGC TCG GCG CTG ATG CAG CAC CCG TTC CAG CCG GTG CGC TTC 9455 GTA TGC CGC AGG CTG TAC CGC CTG CTG CCG GCG CTG CTG TGC GTG 9503 GCG CTG GTC TCG GCG GGG ATG CTG TGG TGG GTG CTG CAG AGC GAC CGC 9551 GCC GAC ATC GCG CTC AAT GGC GCG TAT GCG CTG GTG TAC CTG TCC AAC 9599 ATC TGG GCG TCG GGG CAT GTG GGG TAT TTC CAG GGG CAG GTG GCC 9647 TAT CCG TTC CTG CAC ACG TGG TCG CTG TCG CTG GAA ATG CAG TTC TAC 9695 GCC ATC ATC TTC ATC ATG GCG CTG CTG CTG CCG CTG ACG CGG CAC CGC 9743 AGG CTG GTG CTG TCG GCC ATC TTC GGC GCC TCG TAC TGC GCC 9791 TAT GCC TGG CAT ACG GGT GAC AGC CAG GCC TAT TAC AAC ATC CTC GAC 9839 CGG CTG TGG CAG TTC GCG CTG GGC ACG ATG GTG TGG ATG CTG CCG CGC 9887 CCG AAG CTG CCG CGG GCT GCC GCG GTC GTC GCG GCG GCG GTT 9935 GCG GTG ATC GTG GGG GCA GGG CTG TTC TAC CCC CTC AGC TAC GCG TGC 9983 CCG TCA TGG ATG ACG GTG TTT CCG TGC GGT GCG GTG GTC CTC ATC ATC 10031 ATG CTG CCC GAC ACG CGG GTG G GG CGG TGG TGC CTG GTG CCG CTG TCG 10079 CCG CTG GGG GTG ATC TCG TAT TCG GTC TAT CTG TGG CAC TGG CCG GGC 10127 ATC GTG GTG GCG AAC TAC CTG CTG TTC TTC CAG GTG CAC GGG GCG ATG 10175 ATG GCG GGT GTG CTG GTC ATG GTG GTC AGC CTG CTC AGC TAC 10223 GTC CTT GTC GAA CGC ACC GGC CTC GAT TAC GAG AAC CGC GCC CCC GTC 10271 GCC GCG CGC AAC CGC GGC GCG GCC CTG CTG GTG GCG GCC TGC CTG GGG GTG GTC GTC GCC TAT ATC TCC CAT GTG TCC CGT GTC CAT 10364 TAAGGATGAC ATG ACA CGA CCG CGC GGG CCC GCC CCG CGC GAC GGG GCG 10413 GCG TGG CGG CGT GAC CCC GCG CGC CGG GTG CTG CTG CGT GAC GCG GTG 10461 CGC GGG CGC GAG CGG CTG GCC TGC GCC GTG ATG GCC GGC 10509 CTG ATC GTA TCG GGC GGG GTG GCC TGC GCG CAG GAC AGC CAC ATG GCG 10557 GTG GCG GGT GCG CCC GCC ACC GCG GTC GCG CCG CCC GGG CAG CCC GCC 10605 CCC ATG CCG CCC GCC GTG GCG GAT GCG GCG CAT CTG GCG CAT GCG 10653 GCA GCC GTG CTC GAA CTG CTG CTT GAC CAG GGG TAT TAC TGG CTG GGC 10701 CAG CAT AAC CTG GGC AAG GCG CAC GAG ACG ATC CAG CGC GC G CTG TCC 10749 ATC GAA CCC GAT AAT AAC GAG GCC CTG TTC CTG CAG GGG CGG CTG CAG 10797 ATG GCC GAA GGC GGC ACG ACG CAG GCC ACG CGC ACG CTC GAG CGC CTG 10845 GAG CGC CAG GGG GCG CCG GCG GGG CTG GTG CAC CTG AAG GCG CAG 10893 ATC CAG GCG GGT CCG GTG GAC CCC AGA GCG CTG GCG GAA GCG CGC GCG 10941 CTG GCG GCA TCG GGC AGG ATG ATG CCC GCG ATG TTC AAA TAT AAG GCC 10989 CTG TTC AGG AAC GGC GAC CCG CCG CCC GCC GCG CTG GAA TAT TAC 11037 CGC GTG CTG GGG GCG ACC ATC CTG GGA TAC CAG GAA GCG CGC ACC AGG 11085 CTT GCG GCC TGG GTC GCG CGC AAC CCG CGC GAC ATC GAC GCG CGG CTG 11133 TGC CTG GAC CGG ATC CTG ACC TGT ACC TCG CGC GCC GAG GGG 11181 CTG GAC GGG CTG CGC GCG CTG GCG CGC TCC AAC GTA TCG GCC CAG ATC 11229 CGC AGC GAT GCG GTC GCC GCC TGG CGC GAC GCG CTG CTG TGG GAG CCG 11277 ATC ACG GGG CAG AGC ATC CCTC GAT GAA TGG CTG GCG CAG CAC 11325 CCC GAT GAT ACG GAG TTC ACC ATC CGC CTG AAG AAG GCG CAG GAG ACG 11373 CAG GCT GGC GTT GAC GCG GCG AAT GAC CGC CAG CAG GGC TAT GCG CTG 11421 CTC TCG CGG CAC ATG CTG GAC GCG GCG GCG CGC GAG TTC CAC CGC GCG 11469 GTG GAC ATC GAC CCC CAC GAC CCC GAC GCG CTG GGC GGG CTG GGG CTG 11517 GTG GCG CAG GCA CGC CAG CAG CCG GCG CTG GCG CGG CAG TAT TTC CAG GCC ATG CAG GCC GGT CCC GAT GCG GCC GGA CAC TGG CGC GCG GCG 11613 CTG AAG GCG CTG GAG ACG GGC GGC GGC GGC GTG GAC CCG CTG GTC GCG 11661 CGG ATC GTG CAG GCC ATC AAT GCC GGG CGC TAT GAC GCG GCG CGC 11709 GAC CTT GCC ACG CTG GGC CGC CGG CCG GGC TAT GGG CTG ACG GTG CTG 11757 TCG CTG CAG GCG GCG CTG GCG CGC AGG CAG GGC GAC ACG GCG GAC GCC 11805 GTG CGG CTG TAC CGC GAG GTC GTG CGC CGC GCC CCCC GAT GGG 11853 GCG CTG TTC AGC CTC GGC GCG CTG GAC GTG CAG GTG GGC GAC GCC ACG 11901 GAA GCC GCG GAC ATC CTG ACC CGC CTG CAG CGG CTC GCC CCG GCC ATG 11949 GCG CGG CGG CTG GAG GCG ATG ATG CTG TCG GCG CAGCC CGG GCG 11997 GGG GAT GAT GAC GGG CGC ATC GCC CTG CTG CGC CGC GCG CAG GCG CTG 12045 GAC CCC GAT GAC CCG TGG GTG CGC CTG AAG CTG GCG CAC GCG CTG GAC 12093 GAC GCG GGC GAC CAT GCG GCG GCG CAG GCG ATG ATG GAC GCG CTG ACC 12141 GCG CCA CGC AAT GCC TCC GCG CAG GCC CTG CAG GCG GGC ATC ATC TAC 12189 GCC ATG GGC CGC CAT GAT ACG GCC ACC GCC GGC GCG CTG CTC GAA CGC 12237 ATG CCC CCG ACG CGC ACG CCG GAC ATG GAC CGC CTG GCC AGC CTG 12285 GTG GTG CTG GAC CAG CGG ATT GCC GCC CTT AAC CAC GCG CCC GTG GCC 12333 GGC AAT GCC GCC GTG CTG GCG CTG GCC GAC CAG CCC GAC CCC ACG GGC 12381 AGA CGC GGC CGC ATT GCC GCC GCC CTG CTG GCG CGG CAT GCG CCG 12429 CAG GAT GCG CGC CAG GCG CTG GCG CGC GGG GAA AGC CTG ACG CAG CCG 12477 CCC ACG CCC GCG CGG ATG CTG GCC TAT GCC GGG ACC TAC CTG CGC CTG GCT CCTGCC TTC GAC ACG ACG CGC TGC CTT GAC GCC TTC GAT GCC ATG 12573 GCG AAG GCC CGT CCC GCC GAT GTC ACC GCC GAC CAG GCC CGC GCG CGC 12621 CAA CAG GTC GCC ATC GGC CTT GCG ATC ATG ACC GCC GAC GGG TTC GAC 12669 CGT GGC CGG ACG GCG CAG GCG GCA CAG GTG CTG GCC CCC GTG CTG 12717 CGC GCC CAT CCT GAT TCC GTC GAG GCC CAC CTG GCC ATG GGC CGG GTG 12765 TAC CAG ACC CGC AAC ATG GCC ACG CGC GCG CTG GAG GAG GAC GAG ACG 12813 GCG CTG CGG CTG AAG CCT GCG AAC ATC TAC GCC CTG GCC GCC GCC GCC 12861 CGT GAC GCG GGC GGC GCG CAC CAC CTG GCG CAG GCG AAG GGC TAC GCC 12909 ACC CGT CTG GCG CAC GAG GAC CCC GAT CCG ATG AGC TGG GAA GTC 12957 CGT TCC GAC ATC GAG CGC ATC GAG GGC AAC AGC CGC GGC CAG CTG GCG 13005 GAT GTG GAA CAT GCC CGC CAC GCC CAG TGC ACG CTG GAC GGG GAA GGG 13053 GAA TGC GGC GGG TGC GATC TCC GAT TAC CGC TGG CCC CTC 13101 ATC GAC AGC GAA TAC ATG GAC CTG CAT GGC GCG ACC CTG CCG GCG AGC 13149 TAC CAT TAC ATC CCC GAG GAT GAC GGC GCC CAG GCG ATG GAC CGC CAG 13197 ATC GTC TAC CTG GGT TCC TCG CCG CAG TTC GAC GCC AAT ACC 13245 TTC GTG CGC AGC CGC ACC GGT GTT GCG GGG CTG GGG CAG CTG ACG GAA 13293 TTC GCC GTG CCC ATC ACC GCG ACC CTG CCC TTT GAA TCA TGG GAC CAC 13341 CGG CTG TCC TTC TTC GTC CCG ACC CTG CTG TTC ACC GGC GAT CCG 13389 CTG ACC AAT GCC GTC TCC GCC CAC CAG TTC GGG ACT GTC GCG GTC AAC 13437 GGG GCG CGG CCC TGG GGC TAC CAT CAT TAT TAT ACG CAG GGC GTG GGG 13485 CTG AGC CTG AAT TAC GTC AAC CGC TGG TTC GCG GCC GAC GTG GGC TCC 13533 TCC CCG CTG GGC TTT CCC ATC ACC AAT GTG GTG GGC GGG CTG GAA TTC 13581 GCC CCG CGC CTG ACC CGC AAC CTC GGC CTG CGC GG AGC CGG 13629 CGC ATG GTG ACC GAC AGC GAA CTG TCT TAT GCT GGT GAG CGC GAC CCG 13677 GGC ACC GGA AAG CTG TGG GGC GGG GTG ACG CGG CTG TTC GGC CAT GGC 13725 GCG CTG GAA TGG TCC GCG CGC GGG TGG AAC GCC TCC GGC GGC 13773 TTC GCC TAT CTC GGC GGC ACC AAC GTC ATC GGC AAT ACC GAG ACC GAG 13821 GCC GGC GCC GGC GGC AGC GCC ACC GTG TGG CAG GAC CAC GAC CGG CAG 13869 TGG CTG CGC GTG GGG CTC GAC CTC ATG TATTC GTC AAG CGC AAT 13917 GCC TAC TTC TTT ACA TGG GGG CAG GGC GGA TAC TTC TCG CCC CGG CAG 13965 TAT TTC GGC GCC ATG GTG CCG GTC GAA TGG TCC GGG CAC AAC CGG CGC 14013 TGG ACA TGG TTC CTG CGC GGG GAG CAGCGC TAT TAC CAC AGC 14061 AAC GCC GCG CCG TAT TTC CCG ACC AGC GCG CAG CTG CAG GGC CAG GCC 14109 GAT GGC AGT CCG CCC AGC TAT TAT GGC GAT AGC GGG GCC AGC GGG CTG 14157 GCG GGC AAC AT G CGC GGG CGT CTG GTC TAT CAG CTG GAC CAC CGC CTG 14205 CGC ATC GGG CTG GAA GGC GGC TAC AGC CGC GCG GGC AGC TGG TCG GAG 14253 ACC AGC GGC ATG TGG ATG GCG CAC TAT ACC CTC GAT GGC CAG 14295 TAACCCCGCCGCCAC GTG TCC CGC CAC TTT CGG 14349 GAA CAG CAT GAC ATG ACA CTG CGC AGA CAG GCT TTC GTC CTC ATG GCG 14397 GCC ATG GCG GCC GGC ACG GCA ACG GCG CAC GCG GCG GGG TGC CCG GTG 14445 GAT GCG GGC GGG GAT GCG GTC CCG GCC GCC GAC ACC ACC GCC 14493 GGT GGG CTG GAC CGC CCG CCC CGC GCC GAT GAC GAC GCC CGC CGC GGC 14541 TAC GCG GCA GGC GAC CTG TGG CAG GCG GGG GGG CAG GCC TGG CGG GCG 14589 GTA TCG GTG GCG GCG GCC CGG TGG GCG CGC GTG CCC GCC CGC 14637 CTG CCC GGC GAC GCC TTT GGC GGG CAC ACC GTC TTT GCC GGG GGG CCG 14685 GTG CGG CTG GTC AGC GGC TAT GTC GGG CCC GCC CTT GAT ATC GGG ACG 14733 ACG GTG GCG GGC CGG ACC ACC ACG CTG GCC ATT GGC GGC GAT 14781 GGC CGA CTG GAT ACG GCC GCC CTG CGG CGC GCC CTG TCC GCC CGT GAC 14829 CGC GGC AGC TTC GCC ACC GTG CTG CGG GTG TA T GAC CAG TCC GGC CAT 14877 GGC AAC CAC ATC ACC GCG ACG CCG GGC CGC CAT GTC GTG CAT ATC GGT 14925 GAA ATC CGC ATC GCC GGG CAC GAG ACC CTG TCC TGG GGC GAG GAT AAC 14973 GGT CCC GGC GGC TTC GTG CTG CTC GGG GTG AAG GTC GCA TCC GAC 15021 GGC TTC TTC TTC GGG ACC ACG GGC ACC TAT GCC TCG GCC AAT TCG GGG 15069 AAT GCC GCC GTG CCC GTG CCG GTC CTG CTG GGG CAG GCG GGC AGC GGG 15117 CGG GAG TTC AAG GCC TTG AGC TAC TCG CTC GAC GGG TTC GTC 15165 CAT GTC GCC GAC CCG CGC AGC CCC GAC CAG CGC ACG GGG CTG GTC GTC 15213 ACC TCT TCC CCA GCC GCC TTC GGC GTG GCG GCG GGG CCG GGT GGT TAT 15261 GTG CTG CAG TCG GAT A CGC GCC ACC CTG CCC GGG GCC CTG CCC 15309 GGC GGC ACG CTG GCG GGC GGC TAT ATC GGC TAC AAT GTC GAT GGC GGG 15357 CCA TGG TTC GCG CAG GGA CGC AAT ACG GGA CAG TGG ACC GGC ATC GTC 15405 ATC GCA GGC CAC ACC CCC GAC GAG GTG CAG GCC TTC GCC GTG 15453 GCC GGG GCG GTG GCG GAC GAC GCC ATG CCG CAG CTG CGC GAT GTG CTG 15501 GTC ACC ATC GGC GAC AGC CGC ACG GAA GGC TTC GTG GTG CCG GAT GG C 15549 CGC AAC TGG CCC TAC CTC ATG CAG CGC TTT GCG CGG TAC CGC AGC TAC 15597 AAC CTT GCG GTG TCG GGG GCG ACG ACG CGG CAC ATG CTG GGC ATG CTG 15645 CCC GCG GCC GAA GCC GTC GCC CGC GGG GGC GCG CGG CGG GCC GTG 15693 GTG TTC GGC GGC TAT AAC GAC CAC CTG CCG GGT AAC CAT ATC CCC ACC 15741 GAT GAG ACG GTG CGC AAC CTC GCC ACC ATC ACC GGC CGC CTG AAG CAG 15789 GCG GGC TAC ACC GTC GCC CTC ATC GAG GAG GCG ACT GGC GCC 15837 CCC CGC GCC GCC ATC CAT GAC GCC ATC GCC CGC GGC ATC CTT GCG CCC 15885 GAC ATC GCC CTC GAC CCC TTC GCC CCC GGC CTG CCG CTT GCC AAC GTT 15933 CTC GAC ACC ACC AGA TGG AAC CCT GAC ACC CCC AAC CAG GCC 15981 GGG CAT GAA ATC ATG GCG GAG TTC GTG TGG CAG CAT ATC AGA CCG ATC 16029 TTA AAT AAA TAGCATCGCG ATTACATGTG CCGGATAAGG AGGGAGAGTA 16078 AAAATGCTTT CACACCCAAT ATGGCAGAAA ATACAGTCGT TCCGCATGGA GCATGGCGCC 16138 GAAGGAACGT ATCTGGGCAC CCAGGAGGAC ATGCTGTCCA TGTCGGATGA GGATGTCGAG 16198 GAAATGGTCC GTCTGGGCTG GATCATAGAT GAAGAAAGCG GACGATGGTT CCGCAGGTAT 16258 AAATGACATT CTG CCGGGTA TGCTGCCCCA TCCATGTGCG CAGGAAAACA GGCATGCAGG 16318 CACGCTGCAT GACCTTATGA AATGAATACA GTACAGGTTT TGTGAAATAT AATGCGCCTG 16378 ACCCTTTATA CGGAATATTC CATTCAGACC CTGATTTACC TTGGCAGGAA CCAGGGCAGG 16438 CGTGTGGCTA TTCAGGAAAT TGCTGACACG TATCAGATTT CCTCAAATCA TCTGAGCAAG 16498 ATTGTCTGCC GTCTGGCGCG CAATGGTATG GTCAGCAGCC GCCGTGGCCG TACGGGCGGG 16558 CTGGAACTTG CGCACCCGAC AAACAAGATC ACCCTTGGGG ATATTGTCCG GTTCACGGAA 16618 GCGGACATGG ATGAACTGGT AACCTGTGAA CCGGACAATG GAAAGGCATG CGTTCTGTCG 16678 GATGCCTGCC GGCTGCGCGG CATACTGGCC CAGTCGCTCA ATGCTTTCAT GAGTGTTCTG 16738 GATGGCGTGA CACTTCATGA CGTCATAATG GAATCCGGGC AGCGCCATAT CCATCGGTGA 16798 ATGGCGACCG CGCATGCCGT CTGCCTGATT CCGAATACTT CATTTTGCGG TACGGCAGTA 16858 GCACTGCTGA TCCCCAGATT ACGGGCTGTT ACGCTGGTAT TGCCACGGGA CATGGCCTGT 16918 CCCGCGCGGA TGACATTAGC TGCTTTGGCC TGCTGACAGC CGACCGCTGT CCGTTCTTGC 16978 CATTTCATGC CGGATGGCGG TCTTCATCGC GGCCAGTCAT ATGACAGGGA AACCGTCATA 17038 TTCCGGGTAA TTACAAGCCT GAATGCAGAG GGAAGCAGTA ACCGGCGGGA TCC 17091

SEQ ID NO: 12 Sequence length: 1518 Sequence type: amino acid Topology: linear Sequence type: peptide sequence Met Tyr Gly Thr Trp Phe Thr Thr Gly Lys Val Thr Asp Leu Leu Ala 5 10 15 Arg Thr Gly Leu Asp Arg Val Pro Val Trp Val Pro Val Val Leu Gly 20 25 30 Val Val Leu Met Ala Phe Val Gly Ser Val Arg Ile Asp Pro Ala Leu 35 40 45 Gln Gly Trp Val Ser Val Gly Thr Val Thr Leu Leu Leu Val Leu Asn 50 55 60 Arg Arg Arg Gly Arg Gly Ile Thr Val Phe Leu Met Met Leu Ser Leu 65 70 75 80 Leu Val Ser Leu Arg Tyr Ile Val Trp Arg Leu Thr Ala Thr Val Gln 85 90 95 Phe Ser Asn Trp Leu Gln Thr Ala Leu Ala Val Leu Leu Leu Leu Ala 100 105 110 Glu Ala Tyr Ala Leu Met Thr Leu Cys Leu Ser Tyr Phe Gln Met Ala 115 120 125 Trp Pro Leu Arg Arg Arg Glu His Pro Leu Pro Glu Asp Met Ala Gln 130 135 140 Trp Pro Ser Val Asp Val Phe Val Pro Ser Tyr Asn Glu Glu Leu Ser 145 150 155 160 Leu Val Arg Ser Thr Val Leu Gly Ala Leu Asp Leu Asp Trp Pro Ala 165 170 175 Asp Arg Leu Asn Val Tyr Ile Leu Asp Asp Gly Arg Arg Lys Ala Phe 180 185 190 His Asp Phe Ala Val Glu Ala Gly Ala Gly Tyr Ile Ile Arg Ala Glu 195 200 205 Asn Asn His Ala Lys Ala Gly Asn Leu Asn His Ala Leu Ala Val Thr 210 215 220 Asp Ser Pro Phe Ala Val Ile Phe Asp Cys Asp His Val Pro Thr Arg 225 230 235 240 Gly Phe Leu Arg Arg Thr Ile Gly Trp Met Met Ala Asp Pro Asn Leu 245 250 255 Ala Leu Leu Gln Thr Pro His His Phe Tyr Ala Pro Asp Pro Phe Gln 260 265 270 270 Arg Asn Leu Ala Gly Gly Met His Val Pro Pro Glu Gly Asn Met Phe 275 280 285 Tyr Gly Leu Val Gln Asp Gly Asn Asp Phe Trp Asp Ala Thr Phe Phe 290 295 300 Cys Gly Ser Cys Ala Ile Ile Arg Arg Glu Ala Val Met Gly Ile Gly 305 310 315 320 Gly Phe Ala Thr Glu Thr Val Thr Glu Asp Ala His Thr Ala Leu Lys 325 330 335 Met Gln Arg Arg Gly Trp Gly Thr Ala Tyr Leu Arg Glu Pro Leu Ala 340 345 350 Ala Gly Leu Ala Thr Glu Arg Leu Ile Leu His Ile Gly Gln Arg Val 355 360 365 Arg Trp Ala Arg Gly Met Ile Gln Ile Met Arg Leu Asp Asn Pro Met 370 375 380 380 Leu Gly Ala Gly Leu Arg Trp Glu Gln Arg Leu Cys Tyr Leu Ser Ala 385 390 395 400 Met Ser His Phe Leu Phe Ala Ile Pro Arg Leu Thr Phe Leu Val Ser 405 410 415 Pro Leu Ala Phe Leu Phe Leu Gly Gln Asn Ile Ile Ala Ala Ser Pro 420 425 430 Leu Ala Ile Ser Val Tyr Ala Leu Pro His Ile Phe His Ser Val Ile 435 440 445 Thr Leu Ser Arg Ile Glu Gly Arg Trp Arg Tyr Ser Phe Trp Ser Glu 450 455 460 Ile Tyr Glu Thr Ser Leu Ala Leu Phe Leu Val Arg Ile Thr Ile Val 465 470 475 480 480 Thr Leu Leu Gln Pro His Lys Gly Lys Phe Asn Val Thr Asp Lys Gly 485 490 495 Gly Leu Leu Ala Arg Gly Tyr Phe Asp Trp Asp Ala Val Tyr Pro Asn 500 505 510 Val Ile Leu Ala Gly Val Leu Cys Ala Ala Leu Leu Arg Gly Val Phe 515 520 525 Gly Ile Val Trp Gln Phe His Asp Arg Leu Ala Leu Gln Ser Phe Ile 530 535 540 Leu Asn Thr Leu Trp Val Val Ile Ser Leu Ile Ile Val Leu Ala Ser 545 550 555 560 Ile Ala Val Gly Arg Glu Thr Arg Gln Thr Arg Asn Ala Pro Arg Val 565 570 575 Ser Val Arg Leu Pro Val Val Val Thr Asp Ala His Gly Arg Gln Met 580 585 590 Glu Gly His Thr His Asp Ile Ser Leu Gly Gly Leu Ala Val Gly Thr 595 600 605 Arg Leu Ala Thr Pro Asp Met Val Gly Gly Glu Val Thr Val Arg Tyr 610 615 620 Asp Ser Ala Arg Asp Gly Ile His Val Gly Val Pro Ala Arg Val Leu 625 630 635 640 Asp Ala Arg Asp Gly Thr Leu Arg Leu Arg Trp Ala Val Arg Asp Leu 645 650 655 Glu Asp Glu Arg Gln Val Val Ser Met Val Phe Gly Arg Asn Asp Ala 660 665 670 Trp Ala Gly Trp Ala Asp Phe Ala Pro Asp Arg Pro Leu Arg Ser Leu 675 680 685 Ala Met Val Phe Arg Ser Ile Gly Gly Leu Leu Arg Arg Arg Pro Ala 690 695 700 Glu Ala Pro Arg Ala Leu His Glu Met Gly Glu Gly Glu Leu Pro Ala 705 710 715 715 720 Thr Glu Glu Lys Leu Glu Lys Gln Ser Phe Val Leu Lys Pro Val Pro 725 730 735 Arg Ser Ala Arg His Gly Ala Thr Ala Ser Ala Ala Leu Phe Val Ala 740 745 750 Phe Thr Ala Leu Val Pro Ala Ala Met Ala Gln Glu Ala Pro Ser Pro 755 760 765 Asp Gln Ser Gly Val Thr Ala Glu Thr Pro Phe Gly Asp Ser Asn Thr 770 775 780 Gly Val Val Pro Asp Ala Leu Pro Ala Ile Asp Pro Ala Val Ala Asp 785 790 795 800 Arg Ile Ser Asp Ala Glu Val Thr Arg Thr Leu Thr Phe Arg Asn Leu 805 810 815 Gly Ala Thr Thr Gly Pro Leu Thr Leu Arg Gly Tyr Ser Pro Leu Gln 820 825 830 Gly Leu Asp Val Val Val Pro Ala Asn Arg Val Val Thr His Ala Gln 835 840 845 Leu Thr Leu Ser Gly Ala Leu Ser Pro Ser Leu Leu Pro Glu Ala Ser 850 855 860 Ala Val Thr Val Thr Leu Asn Glu Gln Tyr Val Gly Thr Leu Lys Val 865 870 875 880 Asp Pro Gln His Pro Gln Phe Gly Pro Val Ser Phe Asp Ile Asp Pro 885 890 895 Leu Tyr Phe Thr Gly Asp Asn Lys Leu Asn Phe His Phe Ala Gly Glu 900 905 910 Tyr Arg Arg Asp Cys Asn Asp Leu Phe Asn Glu Ile Leu Trp Ala Arg 915 920 920 925 Ile Ser Asp Met Ser Arg Ile Thr Leu Thr Thr Val Arg Ile Thr Pro 930 935 940 Glu Arg Lys Leu Ser Arg Leu Pro Ala Pro Phe Phe Asp Pro Asn Gln 945 950 955 960 Arg Ser Thr Leu Arg Val Pro Val Val Leu Pro Ala Thr Gly Asp Arg 965 970 975 Gly Ala Leu Arg Ala Ala Gly Leu Val Ala Ser Trp Phe Gly Arg Ile 980 985 990 Ala Asp Phe Arg Lys Leu Ser Phe Pro Val Ser Thr Thr Ile Pro Ala 995 1000 1005 Ser Gly Asn Ala V al Glu Val Gly Val Asn Leu Pro Val Asp Ala Glu 1010 1015 1020 Gly Gly Arg Pro Ala Gly Pro Met Leu Ala Glu Val Ala Asn Pro Asn 1025 1030 1035 1040 Asp Arg Trp Gly Thr Val Leu Val Val Thr Gly Arg Thr Ala Gln Glu 1045 1050 1055 Val Glu Val Ala Ala Arg Ala Leu Val Phe Ser Pro Asp Thr Leu Gly 1060 1065 1070 Gly Val Ala Ser Lys Val Val Ser Asp Val Ser Leu Glu Thr Arg His 1075 1080 1085 Pro Tyr Asp Ala Pro Ala Phe Val Pro Thr Asp Arg Pro Val Arg Phe 1090 1095 1100 Gly Glu Leu Val Gly Ala Ala Asp Leu Gln Gly Gly Gly Phe Ala Pro 1105 1110 1115 1120 Ala Gly Met Thr Leu Pro Phe His Leu Pro Pro Asp Leu Tyr Thr Trp 1125 1130 1135 Arg Gly Arg Pro Phe Leu Met Asn Met Trp Val Arg Ala Pro Gly Gly 1140 1145 1150 Pro Val Val Asp Leu Glu Thr Ser Arg Val Asp Val Ser Leu Asn Asn 1155 1160 1165 Asn Tyr Leu Gln Ser Tyr Thr Leu Ser Pro Pro Gly Leu Trp Arg Lys 1170 1175 1180 Trp Ser Glu Arg Leu Val Asn Gln His Ala Gly Ala Val Gly His Val 1185 1190 1195 1200 Thr Ala Leu Pro Pro Trp Leu Leu Phe Gly Gln Asn Gln Le u Gln Phe 1205 1210 1215 Asn Phe Asp Ala Arg Pro Ile Asp Arg Gly Ala Cys Arg Arg Thr Pro 1220 1225 1230 Gly Asp Ile His Met Ser Val Asp Ser Asp Ser Thr Leu Asp Phe Arg 1235 1240 1245 Arg Gly Tyr His Phe Ala Glu Met Pro Asn Leu Ser Tyr Phe Ala Glu 1250 1255 1260 Ala Ala Phe Pro Phe Ser Arg Met Ala Asp Leu Ser Glu Thr Thr Val 1265 1270 1275 1280 Val Leu Pro Asp His Pro Asp Thr Gly Thr Thr Gly Ala Phe Leu Asp 1285 1290 1395 Leu Met Gly Phe Phe Gly Ala Ser Thr Trp Tyr Pro Ala Ala Gly Val 1300 1305 1310 Thr Val Met Gly Ala Asp Glu Val Ala Gln Thr Pro Pro Lys Gly Asp 1315 1320 1325 Ile Val Val Leu Gly Thr Ala Ala Gln Leu Gly Gly Ala Ala Ser Gly 1330 1335 1340 Leu Leu Ala Arg Ser Pro Tyr Val Ile His Asp Arg His Ile Thr Val 1345 1350 1355 1360 Gly Gln Arg Met Gly Leu Gln Gly Ile Trp Tyr Leu Phe Gln Asp His 1365 1370 1375 Asp His Ala Gly Leu Lys Asp Gly Val Thr Ala Asn Leu Asn Ala Pro 1380 1385 1390 Ile Ala Glu Ala Gly Val Leu Leu Ala Ala Gln Ser Pro Tyr Asp Ser 1395 1400 1405 Gln Arg Ser Val V al Ala Phe Thr Gly Asp Thr Pro Glu Arg Ile His 1410 1415 1420 Asp Leu Val Leu Ser Leu Arg Asn Lys Gly Asp Leu Pro Ser Leu Gln 1425 1430 1435 1440 Gly Asp Leu Val Leu Lys Asn Gly Asp Arg Phe Thr Ser Tyr Arg Thr 1445 1450 1455 Ala Pro Val Tyr Thr Val Gly Ser Leu Pro Leu Trp Leu Arg Leu Asp 1460 1465 1470 Trp Phe Leu Gly His His Pro Ser Ala Leu Tyr Leu Ala Gly Leu Ala 1475 1480 1485 Gly Ala Gly Leu Ala Ala Leu Gly Val Trp Ala Trp Leu Arg Gly Trp 1490 1495 1500 Ser Arg Lys Arg Ile Ala Arg Asp Asp Leu Thr Gly Glu Leu 1505 1510 1515

SEQ ID NO: 13 Sequence length: 386 Sequence type: amino acid Topology: linear Sequence type: peptide sequence Met Leu Gln Leu Asn Pro Thr Pro Pro Ala Pro Gly Arg Trp Arg Thr 5 10 15 Ile Leu Glu Asn Asp Phe Phe Pro Lys Asn Arg Arg Arg Asp Ile Asp 20 25 30 Gly Leu Arg Gly Leu Ala Ile Ala Leu Val Val Leu Phe His Ala Gly 35 40 45 Trp Leu Lys Gly Gly Phe Ile Gly Val Asp Val Phe Val Val Ile Ser 50 55 60 Gly Tyr Phe Met Gly Arg Ser Ala Leu Met Gln His Pro Phe Gln Pro 65 70 75 80 Val Arg Phe Val Cys Arg Arg Leu Tyr Arg Leu Leu Pro Ala Leu Leu 85 90 95 Cys Met Val Ala Leu Val Ser Ala Gly Met Leu Trp Trp Val Leu Gln 100 105 110 Ser Asp Arg Ala Asp Ile Ala Leu Asn Gly Ala Tyr Ala Leu Val Tyr 115 120 125 Leu Ser Asn Ile Trp Ala Ser Gly His Val Gly Tyr Phe Gln Gly Gln 130 135 140 Ala Val Ala Tyr Pro Phe Leu His Thr Trp Ser Leu Ser Leu Glu Met 145 150 155 160 Gln Phe Tyr Ala Ile Ile Phe Ile Met Ala Leu Leu Leu Pro Leu Thr 165 170 175 Arg His Arg Ar g Leu Val Leu Ser Ala Ile Phe Ser Ala Ser Ala Ala 180 185 190 Tyr Cys Ala Tyr Ala Trp His Thr Gly Asp Ser Gln Ala Tyr Tyr Asn 195 200 205 Ile Leu Asp Arg Leu Trp Gln Phe Ala Leu Gly Thr Met Val Trp Met 210 215 220 Leu Pro Arg Pro Lys Leu Pro Arg Ala Ala Ala Asp Ala Val Tyr Ala 225 230 235 240 Ala Ala Val Ala Val Ile Val Gly Ala Gly Leu Phe Tyr Pro Leu Ser 245 250 255 Tyr Ala Cys Pro Ser Trp Met Thr Val Phe Pro Cys Gly Ala Val Val 260 265 270 Leu Ile Ile Met Leu Pro Asp Thr Arg Val Gly Arg Trp Cys Leu Val 275 280 285 Pro Leu Ser Pro Leu Gly Val Ile Ser Tyr Ser Val Tyr Leu Trp His 290 295 300 Trp Pro Gly Ile Val Val Ala Asn Tyr Leu Leu Phe Phe Gln Val His 305 310 315 320 Gly Ala Met Met Ala Gly Val Leu Ala Leu Val Met Val Val Ser Leu 325 330 335 Leu Ser Tyr Val Leu Val Glu Arg Thr Gly Leu Asp Tyr Glu Asn Arg 340 345 350 Ala Pro Val Ala Ala Arg Asn Arg Gly Ala Ala Leu Leu Val Ala Ala 355 360 365 Cys Leu Gly Leu Ala Ala Val Leu Ala Tyr Ile Ser His Val Ser Arg 370 375 380 Val His 385

SEQ ID NO: 14 Sequence length: 1307 Sequence type: amino acid Topology: linear Sequence type: peptide sequence Met Thr Arg Pro Arg Gly Pro Ala Pro Arg Asp Gly Ala Ala Trp Arg 1 5 10 15 Arg Asp Pro Ala Arg Arg Val Leu Leu Arg Asp Ala Val Arg Gly Arg 20 25 30 Glu Gly Gly Leu Arg Leu Ala Cys Ala Val Met Ala Gly Leu Ile Val 35 40 45 Ser Gly Gly Val Ala Cys Ala Gln Asp Ser His Met Ala Val Ala Gly 50 55 60 Ala Pro Ala Thr Ala Val Ala Pro Pro Gly Gln Pro Ala Pro Met Pro 65 70 75 80 Pro Ala Thr Val Ala Asp Ala Ala His Leu Ala His Ala Ala Ala Val 85 90 95 Leu Glu Leu Leu Leu Asp Gln Gly Tyr Tyr Trp Leu Gly Gln His Asn 100 105 110 Leu Gly Lys Ala His Glu Thr Ile Gln Arg Ala Leu Ser Ile Glu Pro 115 120 125 Asp Asn Asn Glu Ala Leu Phe Leu Gln Gly Arg Leu Gln Met Ala Glu 130 135 140 Gly Gly Thr Thr Gln Ala Thr Arg Thr Leu Glu Arg Leu Glu Arg Gln 145 150 155 160 Gly Ala Pro Ala Gly Leu Val Ala Gln Leu Lys Ala Gln Ile Gln Ala 165 170 175 Gly Pro V al Asp Pro Arg Ala Leu Ala Glu Ala Arg Ala Leu Ala Ala 180 185 190 Ser Gly Arg Met Met Pro Ala Met Phe Lys Tyr Lys Ala Leu Phe Arg 195 200 205 Asn Gly Asp Pro Pro Pro Asp Leu Ala Leu Glu Tyr Tyr Arg Val Leu 210 215 220 Gly Ala Thr Ile Leu Gly Tyr Gln Glu Ala Arg Thr Arg Leu Ala Ala 225 230 235 240 Trp Val Ala Arg Asn Pro Arg Asp Ile Asp Ala Arg Leu Cys Leu Asp 245 250 255 Arg Ile Leu Thr Tyr Arg Val Thr Ser Arg Ala Glu Gly Leu Asp Gly 260 265 270 270 Leu Arg Ala Leu Ala Arg Ser Asn Val Ser Ala Gln Ile Arg Ser Asp 275 280 285 Ala Val Ala Ala Ala Trp Arg Asp Ala Leu Leu Trp Glu Pro Ile Thr Gly 290 295 300 Gln Ser Ile Pro Leu Tyr Asp Glu Trp Leu Ala Gln His Pro Asp Asp 305 310 315 320 Thr Glu Phe Thr Ile Arg Leu Lys Lys Ala Gln Glu Thr Gln Ala Gly 325 330 335 Val Asp Ala Ala Asn Asp Arg Gln Gln Gly Tyr Ala Leu Leu Ser Arg 340 345 350 His Met Leu Asp Ala Ala Ala Arg Glu Phe His Arg Ala Val Asp Ile 355 360 365 Asp Pro His Asp Pro Asp Ala Leu Gly Gly Leu Gly Leu Val Ala Gln 370 375 380 Ala Arg Gln G ln Pro Ala Leu Ala Arg Gln Tyr Phe Leu Gln Ala Met 385 390 395 400 Gln Ala Gly Pro Asp Ala Ala Gly His Trp Arg Ala Ala Leu Lys Ala 405 410 415 Leu Glu Thr Gly Gly Gly Gly Val Asp Pro Leu Val Ala Arg Ile Val 420 425 430 Gln Ala Ile Asn Ala Gly Arg Tyr Asp Ala Ala Arg Ala Asp Leu Ala 435 440 445 Thr Leu Gly Arg Arg Pro Gly Tyr Gly Leu Thr Val Leu Ser Leu Gln 450 455 460 Ala Ala Leu Ala Arg Arg Gln Gly Asp Thr Ala Asp Ala Val Arg Leu 465 470 475 480 Tyr Arg Glu Val Val Arg Arg Ala Pro Arg Asp Ala Gly Ala Leu Phe 485 490 495 Ser Leu Gly Ala Leu Asp Val Gln Val Gly Asp Ala Thr Glu Ala Ala 500 505 510 Asp Ile Leu Thr Arg Leu Gln Arg Leu Ala Pro Ala Met Ala Arg Arg 515 520 525 Leu Glu Ala Met Met Leu Ser Ala Gln Ala Asp Arg Ala Gly Asp Asp 530 535 540 Asp Gly Arg Ile Ala Leu Leu Arg Arg Ala Gln Ala Leu Asp Pro Asp 545 550 555 560 Asp Pro Trp Val Arg Leu Lys Leu Ala His Ala Leu Asp Asp Ala Gly 565 570 575 Asp His Ala Ala Ala Ala Gln Ala Met Met Asp Ala Leu Thr Ala Pro Arg 580 585 590 590 Asn Ala Ser A la Gln Ala Leu Gln Ala Gly Ile Ile Tyr Ala Met Gly 595 600 605 Arg His Asp Thr Ala Thr Ala Gly Ala Leu Leu Glu Arg Met Pro Arg 610 615 620 620 Thr Gly Arg Thr Pro Asp Met Asp Arg Leu Ala Ser Leu Val Val Leu 625 630 635 640 Asp Gln Arg Ile Ala Ala Leu Asn His Ala Pro Val Ala Gly Asn Ala 645 650 655 Ala Val Leu Ala Leu Ala Asp Gln Pro Asp Pro Thr Gly Glu Arg Gly 660 665 670 Met Arg Ile Ala Ala Ala Leu Leu Ala Arg His Ala Pro Gln Asp Ala 675 680 685 Arg Gln Ala Leu Ala Arg Gly Glu Ser Leu Thr Gln Pro Pro Thr Pro 690 695 700 Ala Arg Met Leu Ala Tyr Ala Gly Thr Tyr Leu Arg Leu Arg Ser Ala 705 710 715 720 Phe Asp Thr Thr Arg Cys Leu Asp Ala Phe Asp Ala Met Ala Lys Ala 725 730 735 Arg Pro Ala Asp Val Thr Ala Asp Gln Ala Arg Ala Arg Gln Gln Val 740 745 750 Ala Ile Gly Leu Ala Ile Met Thr Ala Asp Gly Phe Asp Arg Tyr Gly 755 760 765 Arg Thr Ala Gln Ala Ala Gln Val Leu Ala Pro Val Leu Arg Ala His 770 775 780 Pro Asp Ser Val Glu Ala His Leu Ala Met Gly Arg Val Tyr Gln Thr 785 790 795 800 Arg Asn Met A la Thr Arg Ala Leu Glu Glu Asp Glu Thr Ala Leu Arg 805 810 815 Leu Lys Pro Ala Asn Ile Tyr Ala Leu Ala Ala Ala Ala Arg Asp Ala 820 825 830 Gly Gly Ala His His Leu Ala Gln Ala Lys Gly Tyr Ala Thr Arg Leu 835 840 845 Ala His Glu Asp Pro Asp Gly Pro Met Ser Trp Glu Val Arg Ser Asp 850 855 860 Ile Glu Arg Ile Glu Gly Asn Ser Arg Gly Gln Leu Ala Asp Val Glu 865 870 870 875 880 His Ala Arg His Ala Gln Cys Thr Leu Asp Gly Glu Gly Glu Cys Gly 885 890 895 Gly His Glu Ser Phe Val Ser Asp Tyr Arg Trp Pro Leu Ile Asp Ser 900 905 910 Glu Tyr Met Asp Leu His Gly Ala Thr Leu Pro Ala Ser Tyr His Tyr 915 920 925 Ile Pro Glu Asp Asp Gly Ala Gln Ala Met Asp Arg Gln Ile Val Tyr 930 935 940 Leu Arg Asp Ser Val Ser Pro Gln Phe Asp Ala Asn Thr Phe Val Arg 945 950 955 960 Ser Arg Thr Gly Val Ala Gly Leu Gly Gln Leu Thr Glu Phe Ala Val 965 970 975 Pro Ile Thr Ala Thr Leu Pro Phe Glu Ser Trp Asp His Arg Leu Ser 980 985 990 Phe Ser Val Thr Pro Thr Leu Leu Phe Thr Gly Asp Pro Leu Thr Asn 995 1000 1005 Ala Val Ser Ala His Gln Phe Gly Thr Val Ala Val Asn Gly Ala Arg 1010 1015 1020 1025 1030 1035 1040 Asn Tyr Val Asn Arg Trp Phe Ala Ala Asp Val Gly Ser Ser Pro Leu 1045 1050 1055 Gly Phe Pro Ile Thr Asn Val Val Gly Gly Leu Glu Phe Ala Pro Arg 1060 1065 1070 Leu Thr Arg Asn Leu Gly Leu Arg Ile Ser Gly Gly Arg Arg Met Val 1075 1080 1085 Thr Asp Ser Glu Leu Ser Tyr Ala Gly Glu Arg Asp Pro Gly Thr Gly 1090 1095 1100 Lys Leu Trp Gly Gly Val Thr Arg Leu Phe Gly His Gly Ala Leu Glu 1105 1110 1115 1120 Trp Ser Ala Arg Gly Trp Asn Ala Tyr Ala Gly Gly Gly Phe Ala Tyr 1125 1130 1135 Leu Gly Gly Thr Asn Val Ile Gly Asn Thr Glu Thr Glu Ala Gly Ala 1140 1145 1150 Gly Gly Ser Ala Thr Val Trp Gln Asp His Asp Arg Gln Trp Leu Arg 1155 1160 1165 Val Gly Leu Asp Leu Met Tyr Phe Gly Tyr Lys Arg Asn Ala Tyr Phe 1170 1175 1180 Phe Thr Trp Gly Gln Gly Gly Tyr Phe Ser Pro Arg Gln Tyr Phe Gly 1185 1190 1195 1200 Ala Met Val Pro Val Glu Trp Ser Gly His Asn Arg Arg Trp Thr Trp 1205 1210 1215 Phe Leu Arg Gly Glu Ala Gly Tyr Gln Tyr Tyr His Ser Asn Ala Ala 1220 1225 1230 Pro Tyr Phe Pro Thr Ser Ala Gln Leu Gln Gly Gln Ala Asp Gly Ser 1235 1240 1245 Pro Pro Ser Tyr Tyr Gly Asp Ser Gly Ala Ser Gly Leu Ala Gly Asn 1250 1255 1260 Met Arg Gly Arg Leu Val Tyr Gln Leu Asp His Arg Leu Arg Ile Gly 1265 1270 1275 1280 Leu Glu Gly Gly Tyr Ser Arg Ala Gly Ser Trp Ser Glu Thr Ser Gly 1285 1290 1295 Met Trp Met Ala His Tyr Thr Leu Asp Gly Gln 1300 1305

SEQ ID NO: 15 Sequence length: 569 Sequence type: amino acid Topology: Linear Sequence type: Peptide sequence Val Ser Arg His Phe Arg Glu Gln His Asp Met Thr Leu Arg Arg Gln 5 10 15 Ala Phe Val Leu Met Ala Ala Met Ala Ala Gly Thr Ala Thr Ala His 20 25 30 Ala Ala Gly Cys Pro Val Asp Ala Gly Gly Asp Thr Val Cys Val Pro 35 40 45 Ala Ala Asp Thr Thr Ala Gly Gly Leu Asp Arg Pro Pro Arg Ala Asp 50 55 60 Asp Asp Ala Arg Arg Gly Tyr Ala Ala Gly Asp Leu Trp Gln Ala Gly 65 70 75 80 Gly Gln Ala Trp Arg Ala Val Ser Val Ala Asp Gly Ala Ala Arg Trp 85 90 95 Ala Arg Val Pro Ala Arg Leu Pro Gly Asp Ala Phe Gly Gly His Thr 100 105 110 Val Phe Ala Gly Gly Pro Val Arg Leu Val Ser Gly Tyr Val Gly Pro 115 120 125 Ala Leu Asp Ile Gly Thr Thr Val Val Ala Gly Arg Thr Val Thr Thr Thr 130 135 140 Leu Ala Ile Gly Gly Asp Gly Arg Leu Asp Thr Ala Ala Leu Arg Arg 145 150 155 160 Ala Leu Ser Ala Arg Asp Arg Gly Ser Phe Ala Thr Val Leu Arg Val 165 170 175 Tyr Asp Gln Se r Gly His Gly Asn His Ile Thr Ala Thr Pro Gly Arg 180 185 190 His Val Val His Ile Gly Glu Ile Arg Ile Ala Gly His Glu Thr Leu 195 200 205 Ser Trp Gly Glu Asp Asn Gly Pro Gly Gly Phe Val Leu Pro Asp Gly 210 215 220 Val Lys Val Ala Ser Asp Gly Phe Phe Phe Gly Thr Thr Gly Thr Tyr 225 230 235 240 Ala Ser Ala Asn Ser Gly Asn Ala Ala Val Pro Val Pro Val Leu Leu 245 250 255 Gly Gln Ala Gly Ser Gly Arg Glu Phe Lys Ala Phe Met Gly Ser Tyr 260 265 270 270 Ser Leu Asp Gly Phe Val His Val Ala Asp Pro Arg Ser Pro Asp Gln 275 280 285 Arg Thr Gly Leu Val Val Thr Ser Ser Pro Ala Ala Phe Gly Val Ala 290 295 300 Ala Gly Pro Gly Gly Tyr Val Leu Gln Ser Gly Asn Ala Arg Ala Thr 305 310 315 320 Leu Pro Gly Ala Leu Pro Gly Gly Thr Leu Ala Gly Gly Tyr Ile Gly 325 330 335 Tyr Asn Val Asp Gly Gly Pro Trp Phe Ala Gln Gly Arg Asn Thr Gly 340 345 350 Gln Trp Thr Gly Ile Val Ile Ala Gly His Ala Pro Thr Pro Asp Glu 355 360 365 Val Gln Ala Phe Ala Val Ala Gly Ala Val Ala Asp Asp Ala Met Pro 370 375 380 380 Gln Leu Arg Asp Va l Leu Val Thr Ile Gly Asp Ser Arg Thr Glu Gly 385 390 395 400 400 Phe Val Val Pro Asp Gly Arg Asn Trp Pro Tyr Leu Met Gln Arg Phe 405 410 415 Ala Arg Tyr Arg Ser Tyr Asn Leu Ala Val Ser Gly Ala Thr Thr Arg 420 425 430 His Met Leu Gly Met Leu Pro Ala Ala Glu Ala Val Ala Arg Gly Gly 435 440 445 Ala Arg Arg Leu Ala Val Val Phe Gly Gly Tyr Asn Asp His Leu Pro 450 455 460 Gly Asn His Ile Pro Thr Asp Glu Thr Val Arg Asn Leu Ala Thr Ile 465 470 475 480 Thr Gly Arg Leu Lys Gln Ala Gly Tyr Thr Val Ala Leu Ile Glu Glu 485 490 495 Ala Gln Thr Thr Gly Ala Pro Arg Ala Ala Ile His Asp Ala Ile Ala 500 505 510 Arg Gly Ile Leu Ala Pro Asp Ile Ala Leu Asp Pro Phe Ala Pro Gly 515 520 525 Leu Pro Leu Ala Asn Val Leu Asp Thr Arg Trp Asn Pro Asp Thr 530 535 540 Thr His Pro Asn Gln Ala Gly His Glu Ile Met Ala Glu Phe Val Trp 545 550 555 560 Gln His Ile Arg Pro Ile Leu Asn Lys 565

[0051]

[Brief description of the drawings]

FIG. 1 is a diagram showing a restriction map of pCEL1, pCEL2, and pCEL3.

FIG. 2: Acetobacter xylinum JCM7 of the present invention
It is a figure which shows the base sequence of the cellulose synthase gene derived from 664 strain.

FIG. 3 is an Acetobacter xylinum JCM7 of the present invention.
FIG. 3 is a view showing a base sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 2.

FIG. 4: Acetobacter xylinum JCM7 of the present invention
FIG. 4 is a view showing a base sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 3.

FIG. 5: Acetobacter xylinum JCM7 of the present invention
FIG. 5 is a view showing the nucleotide sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 4.

FIG. 6: Acetobacter xylinum JCM7 of the present invention
FIG. 6 is a view showing a base sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 5.

FIG. 7: Acetobacter xylinum JCM7 of the present invention
FIG. 7 is a view showing the nucleotide sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 6.

FIG. 8: Acetobacter xylinum JCM7 of the present invention
FIG. 8 is a view showing a base sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 7.

FIG. 9: Acetobacter xylinum JCM7 of the present invention
FIG. 9 is a view showing a base sequence of a cellulose synthase gene derived from 664 strain, and is a continuation of FIG. 8.

FIG. 10: Acetobacter xylinum JCM of the present invention
FIG. 10 is a view showing a nucleotide sequence of a cellulose synthase gene derived from strain 7664, and is a continuation of FIG. 9.

FIG. 11 is a diagram comparing the sequence of the boundary region between the homologous part and the different part of pCEL1 and pCEL2.

FIG. 12: Acetobacter xylinum JCM7664
FIG. 3 shows the homology of the bcsII operon of the strain.

FIG. 13: Acetobacter xylinum JCM7664
It is the figure which compared the amino acid sequence of BcsY derived from a strain, and transacylase derived from other microorganisms.

FIG. 14: Acetobacter xylinum JCM7664
It is a figure which compared hydropathy of BcsY derived from a strain and OafA derived from Salmonella.

FIG. 15: Acetobacter xylinum JCM7664
It is the figure which compared the ORF of BcsY derived from a strain and OafA derived from Salmonella.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Yasuyoshi Honami 4-1 Egasakicho, Tsurumi-ku, Yokohama-shi, Kanagawa Prefecture Inside the Energy and Environment Research Laboratory, Tokyo Electric Power Company (72) Inventor Shunji Kunigami Kanagawa Prefecture 4-1, Egasaki-cho, Tsurumi-ku, Yokohama-shi Tokyo Electric Power Company Energy and Environment Research Laboratory (72) Inventor Hideo Akiyama 1111 Tehiro, Kamakura-shi, Kanagawa Prefecture East Research Center Biological Sciences Research Division (72) Invention Takuo Onizuka 1111 Tehiro, Kamakura-shi, Kanagawa Pref. Biological Sciences Research Division, East Research Center Co., Ltd. (72) Inventor Masahiko Ikeuchi 3-8-1, Komaba, Meguro-ku, Tokyo Faculty of Liberal Arts, Tokyo University (72) Inventor Inoue Yori Nao 2-1 Hirosawa, Wako-shi, Saitama RIKEN Photosynthesis Science Laboratory

Claims (13)

[Claims] 1. A DNA comprising the nucleotide sequence of positions 9207 to 10364 or the complementary sequence thereof and a part or all of these sequences in the nucleotide sequence shown in SEQ ID NO: 11. 2. A DNA derived from an acetic acid bacterium which hybridizes with the DNA according to claim 1 under stringent conditions. 3. A DNA encoding a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 13. 4. A DNA encoding a polypeptide comprising an amino acid sequence in which one or several amino acids have been deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 13. 5. A DNA comprising the nucleotide sequence from position 3745 to position 8298 of the nucleotide sequence shown in SEQ ID NO: 11, or a complementary sequence thereof, and a part or all of these sequences. 6. A DNA derived from an acetic acid bacterium that hybridizes with the DNA according to claim 5 under stringent conditions. 7. A DNA encoding a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 12. 8. A DNA encoding a polypeptide comprising an amino acid sequence in which one or several amino acids have been deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 12. 9. A DNA comprising the base sequence from the 10375th position to the 14295th position in the base sequence shown in SEQ ID NO: 11 or a complementary sequence thereof and a part or all of these sequences. 10. A DN derived from acetic acid bacteria which hybridizes with the DNA according to claim 9 under stringent conditions.
A. 11. A DNA encoding a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 14. 12. A DNA encoding a polypeptide consisting of an amino acid sequence in which one or several amino acids have been deleted, substituted or added in the amino acid sequence shown in SEQ ID NO: 14. 13. A DNA comprising the base sequence shown in SEQ ID NO: 11 or a sequence complementary thereto and a part or all of these sequences.