JPH1092A - New nucleic acid fragment, primer consisting thereof and detection of hepatitis non-a-b-c-d-e-f virus using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new nucleic acid fragment, a specific base sequenceconsecutive nucleic acid fragment, characteristic of hepatitis nonA-B-C- D-E-F virus, thus useful as a primer for detecting the virus. SOLUTION: This new nucleic acid fragment, which is composed of a nucleotide consisting of consecutive at least NO nucleotides with a base sequence of formula I or formula II and has a base sequence of characteristic domain useful for classifying hepatitis non-A-B-C-D-E-F virus, is useful as e.g. a primer to be used for e.g. detecting the virus. This new nucleic acid fragment is obtained by the following process: as a result of study through collecting the blood specimens from patients suffered from hepatitis throughout the world, a number of new hepatitis non-A-B-C-D-E-F virus's subtypes are discavered, and found to be classifiable based on the base sequence of the 5' non-translation domain of the virus, the base sequence is determined, and a partial sequence thereof is synthesized.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a novel nucleic acid fragment, a primer comprising the same, and a non-ABC-
Method for detecting DEF hepatitis virus and non-AB-
The present invention relates to a method for classifying CDEF hepatitis virus (which may be referred to as “HGV” or “GBV” in the present specification and the drawings).

[0002]

2. Description of the Related Art Conventionally, hepatitis viruses include types A and B.
Types, C type, D type, E type and F type are known. Recently,
A new type of hepatitis virus that does not belong to any of these types has been discovered and is called hepatitis G virus (HGV) or GB virus (GBV). In the present specification, A
Hepatitis viruses that do not belong to any of the types B, C, D, E and F (including those called HGV and GBV) are non-ABCD- Called hepatitis EF virus.

[0003]

DISCLOSURE OF THE INVENTION The present invention provides a novel non-ABCDCEF hepatitis virus which has not been known so far, and a characteristic region useful for its classification. Is determined, and based on it, the non-ABC-
An object of the present invention is to provide a primer useful for the detection of a DEF hepatitis virus and a method for detecting a non-ABCDCEF hepatitis virus using the same.

[0004]

DISCLOSURE OF THE INVENTION The present inventors have collected and studied blood samples of hepatitis patients from all over the world, and as a result, have found that many novel non-ABCDCEF types of hepatitis viruses. Subtypes and their non-ABCCD
The present invention has been completed by finding out that hepatitis EF virus can be classified based on the nucleotide sequence of the 5 ′ untranslated region, and determining those nucleotide sequences. Furthermore, the present inventors also determined the nucleotide sequences of the NS5b region and NS3 region of the non-ABCDCEF hepatitis virus, and prepared primers suitable for amplifying these regions. I found it.

[0006] That is, the present invention relates to SEQ ID NO: 1 in the sequence listing.
And a nucleic acid fragment comprising 10 or more consecutive nucleotides of the nucleotide sequence shown in any one of (1) to (144) and (162) to (355). Also, the present invention
A non-ABCDE comprising the nucleic acid fragment of the present invention.
-To provide a primer for detecting hepatitis F virus. Further, the present invention provides the above-mentioned primer of the present invention at least 2
The present invention provides a method for detecting non-ABCDCEF hepatitis, which comprises performing PCR using a plurality of cells and detecting an amplification product. Further, the present invention provides a non-ABC comprising a nucleic acid having a base sequence represented by SEQ ID NO: 359 in the sequence listing.
A probe for detecting the NS5A region of the hepatitis D-F virus is provided. Furthermore, the present invention relates to a non-ABCDCEF comprising a nucleic acid having a base sequence represented by any one of SEQ ID NOs: 360 to 362 in the sequence listing.
A probe for detecting the 5 'untranslated region of hepatitis virus is provided. Furthermore, the present invention relates to a non-ABCDE-
The 5 'untranslated region of hepatitis F virus was replaced with BsmF1, B
Digestion was performed with each restriction enzyme having a cleavage site for smA1 and ScrFI, and based on the cleavage pattern, non-ABC-
Non-A-, which classifies DEF hepatitis virus into four types
The present invention provides a method for classifying BCDDEF hepatitis virus.

[0007]

DETAILED DESCRIPTION OF THE INVENTION As described above, the present inventors have:
As a result of collecting and studying blood samples of hepatitis patients from all over the world, we have found a number of novel non-ABCDCEF hepatitis virus subtypes and their 5 'untranslated regions,
The nucleotide sequences of the NS3 and NS5b regions were determined. These determined new nucleotide sequences are represented by SEQ ID NO: 1 in the sequence listing.
144 to 162 to 355. In addition, SEQ ID NOs: 1 to 45, 189 to 207, 281 to 321 and 331 to 342 are 5 'untranslated regions, SEQ ID NOs: 46 to 92.
And 253-270 and 277-278 are NS3 regions,
SEQ ID NOs: 93 to 144 and 162 to 188 and 208 to
252 and 271-276 and 279-280 and 32
2-330 and 343-355 show the nucleotide sequence of the NS5b region. The positions of the 5 ′ untranslated region, NS3 region and NS5b region in the virus genome are known, and are described, for example, in MUERHOFF, AS et al., Journal of Virol.
ogy (1995), 69, 5621-5630.

The newly discovered nucleotide sequence can be specifically detected by using a nucleic acid fragment that hybridizes with a part or the entire region of the nucleotide sequence as a primer or probe for PCR. . The nucleic acid fragment is preferably a DNA fragment, but an RNA fragment can also be used. The number of bases of a nucleic acid fragment used as a primer or a probe is preferably 10 or more, more preferably 15 or more. In the case of a primer, the number of bases of the nucleic acid fragment is 1
Particularly preferred is about 5 to 50. The primer may be labeled with biotin, dioxygenin, a radiolabel, an enzyme label, a fluorescent label, or the like. Such a label is required in the case of a probe.

[0009] The PCR (RT-PCR) method itself and the detection method using a probe are well known.
In addition, using these, RFLP,
Non-ABCCD by PCR-RFLP, SSCP, etc.
-Detection and / or classification of hepatitis EF virus can be performed.

[0010] The inventors of the present application have also found particularly high detection sensitivity as primers for PCR. These base sequences are represented by SEQ ID NOs: 145 to 161 and 3 in the sequence listing.
56 to 358. Among them, SEQ ID NO: 1
45 to 153 are primers for amplifying 5 'non-translated region, SEQ ID NOs: 154 to 156 are NS5b region amplifying primers, 356 to 358 are NS5A region amplifying primers, and SEQ ID NOs: 157 to 161 are NS3 region amplifying primers. . Table 1 below shows the relationship among the names, the sequence numbers, and the amplification regions assigned to these primers.

[0011]

[Table 1]

When amplifying a target nucleic acid by PCR, at least one pair of primers is required. The present inventors have also found a set of primers having particularly excellent detection sensitivity. The names of these primer sets and the names of the primers used (see Table 1) are shown below.

Primer set A1 MUGR1 MUGF1 Primer set A2 MUGR2 MUGF1 Primer set B1 5gr4AD 5gf2-2 Primer set B2 5gr4AD 5gf3 Primer set C1 MG5BF3 MUNS5R1 Primer set C2 MG5BF4 MUNS5R5 AR5 AR5 Primer AR5D5AR1 Primer set

When performing the PCR, the inventors of the present invention include a known amount of another nucleic acid fragment (competitor) to be amplified by a primer to be used in a sample, and the size of the amplified product is not larger than that of the target nucleic acid. And the competitor is different, by comparing the thickness of the amplified competitor band and the thickness of the amplified target nucleic acid,
It has been found that the amount of the target nucleic acid can be estimated, and whether or not a slight difference in the base sequence between the primer and the target nucleic acid exists (that is, whether or not there is a false positive) can be known.
An example of this method is described in the examples below.

That is, a competitor having a primer sequence for amplification of a gene to be detected in a sample and having a gene (sequence) different from the gene sequence to be detected sandwiched between primer sequences is prepared.
This method is a method of relatively quantifying a target gene by quantitatively detecting the amount of a competitor's amplification product with a probe having a nucleotide sequence that the competitor has but does not have the amplification product of the gene to be detected. . When the amount of the target gene increases (decreases) by simultaneously adding a fixed amount of the competitor together with the sample at the time of cDNA synthesis or PCR, the detected amount of the competitor decreases (increases).

When quantitatively measuring a gene having a large number of mutations such as an RNA virus, a mutation may be caused in the sequence of a gene to be detected or quantified in a sequence used as a probe. If the sequence of the probe and the nucleotide sequence of the gene region complementary to the probe of the gene to be quantified are different even by one nucleotide, the efficiency of hybridization is reduced, and the detection value is expected to be lower than the actual value. You. Therefore, rather than capturing the amplified virus itself with a probe, it becomes possible to quantitatively measure the competitor using a specific foreign gene as a competitor and to quantify it relatively accurately.

The present inventors have newly found a non-AB
As a result of detailed examination of the 5 ′ untranslated region of the hepatitis virus subtype, the non-ABDCEF type was determined by the fragment length pattern after digestion with the restriction enzyme. It has been found that hepatitis virus can be classified into four types. This is the usual method of RFLP or PCR-RFLP
Can be performed.

The four types are 1, 2, 3a and 3b, and the classification criteria are as follows.

1 is cleaved with ScrFI to give approximately 108
A fragment of 40, 34 bp is generated, and about 108 or 40 fragments are detected.

2 is uncleaved or cleaved with ScrFI, yielding fragments of about 149, 34 bp and detecting fragments of 184 or 149 bp. Further, fragments of 157 and 26 bp which are digested with BsmFI are detected.

3a is uncleaved or cleaved with ScrFI, yielding fragments of approximately 149, 34 bp, and detecting fragments of 184 or 149 bp. And not cut by BsmFI and cut by BsmAI to about 75, 55, 5
Generate a 3 bp fragment and detect a 75 or 53 bp fragment.

3b is uncleaved or cleaved with ScrFI, yielding fragments of about 149, 34 bp and detecting fragments of 184 or 149 bp. Not cut with BsmFI. Not cut by BsmAI or cut by about 1
A 28, 55 bp fragment is detected.

However, it is not limited to these restriction enzymes. Since there are different restriction enzymes that recognize the same sequence, such different restriction enzymes can also be used.
For example, BsmF1 and FinI recognize the same restriction enzyme site, so that FinI can be used instead of BsmFI.

More specifically, the relationship between the sequence in the vicinity of each restriction enzyme site and each type is as follows. The type determination method is
Any method may be used as long as it detects these type-specific sequences, and is not limited to restriction enzymes. For example, a probe or a type-specific primer may be used.

ScrFI recognition site type sequence 1 GT-AATAAGGRCCCGGCGCTAGGCACGCC 2,3a, 3b GTGAATAAGGGCCCGACGTCAGGCTCGTC

BsmF1 recognition sequence type sequence 1 TTAARGGGAGGCTACGGTCCCTCTTGCGCTTATG 2 TTAAGGGTTGGTYAAGGTCCCTCTGGCGCTTGTG 3a TTAAGGGAAGGTYAAGATTCCTCTTGTGCCTGTG 3b TTAAGAGAAGGTTAAGATTCCTCTTGTGCCTGCG

BsmAI recognition site type sequence 1 AAACCGAGHCC 2 AAACCGAGCCC 3a AAACCGAGACC 3b AAACCGAGCCC

[0028]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below more specifically based on embodiments. However, the present invention is not limited to the following examples.

Example 1 Determination of Nucleotide Sequence of Non-ABCDCEF Hepatitis Virus According to the method described in Example 11 below, non-A-C-D-EF 5 'untranslated region, NS3 region and NS5 of BCDDEF hepatitis virus
The base sequence of the b region was determined. As a result, the nucleotide sequences represented by SEQ ID NOs: 1 to 355 in the sequence listing were determined. Moreover, as a result of phylogenetic analysis, classification was possible as shown in FIGS.
The homology is as shown in Table 14.

[Table 14]

Example 2 High-sensitivity detection system Comparison with existing primers Reference of existing primers; M. Yoshiba, et al, Detection of GBV-C hepatitis vorus
genome in serum frompatients with fulminant hepat
itis of unknown aetiology, The Lancet, 28,1131-1132,
1995).

Samples Saudi Arabia 108 patients with chronic hepatitis P.R.China 50 patients with liver disease, 30 blood donors, 120 patients with Japanese liver disease, 87 other diseases (autoimmune disease, fatty liver, etc.)

Method 1) Purification of RNA 100 μl of serum was added to RNAzolB (trade name, Biotex Lab)
900 μl) and 150 μl of chloroform
After stirring in a 2 ml Eppendorf tube for 15 seconds, 4
It was left at ℃ for 5 minutes. The mixture was centrifuged at 12000 rpm at 4 ° C. for 15 minutes, and the supernatant was transferred to a 1.5 ml Eppendorf tube. 650 μl of isopropanol was added thereto, and the mixture was stirred and left for 15 minutes, followed by centrifugation at 12,000 rpm at 4 ° C. for 20 minutes to obtain a precipitate. Further, 1 ml of 75% ethanol was added, and the mixture was stirred and centrifuged at 12,000 rpm at 4 ° C. for 5 minutes to discard the supernatant. After repeating this operation two more times, the obtained precipitate was dried.

2) cDNA synthesis 5 × RT buffer (trade name; Life T) was added to the purified RNA.
(echnologies, Inc.) 2 μl, 0.1 M DTT 1 μl, 10 mM dNT
P 1 μl, Random Primer (Product name; Life Technologies
Inc. 9OD 260 nm) 0.1 μl, MMLV-reverse transcriptase (trade name; Life Technologies Inc.) 50 units, ribonuclease inhibitor (trade name; Takara Shuzo) 20 units, DE
After adding water treated with PC and stirring with a total of 10 μl, the mixture was incubated at 37 ° C. for 1 hour.

3) PCR In order to increase the detection sensitivity, in the first PCR, the above cDNA was subjected to PCR amplification (Saiki et al., Science 239, 481-491, 1988) using the primer set A1. The reaction solution was 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl2, 50 mM KC
l, 800 μM dNTP, 1.25 units AmpliTaq DNA polymerase
(Product name; Japan ROCHE). PCR conditions were cycle 35, denaturation 94 ° C, 20 seconds, annealing 50 ° C, 20
The extension was performed at 72 ° C. for 20 seconds. Further, this amplification product was subjected to a second PCR amplification using the primer set A2. The PCR conditions were cycle 35, denaturation 94
℃, 20 seconds, annealing 60 ℃, 20 seconds, extension 72 ℃, 2
Performed at 0 seconds. Experiments using known primers are described in M. Yosh
Amplification primers of New viruses (GBV-A, B, C) described in iba et al. RNA purification and cDNA synthesis were performed as described above, and PCR was performed as in the literature.

4) Electrophoresis The amplified product was electrophoresed on agarose (triborate buffer, 0.5 μg / ml ethidium bromide) (1
50V constant current). After electrophoresis, under ultraviolet irradiation,
Polaroid (Polaroid type 667) photography was performed.
For the amplification products of the primer sets A1 and 2, an amplification band of about 170 bp was confirmed with reference to the DNA size marker.

Results Primer sets A1 and A2 for liver disease in the following three countries:
Had a higher detection rate than the known method (Table 2). The amplification product using the new primer is transferred to a quick spin column G50.
(TE) (trade name; manufactured by Boehringer Mannheim). Furthermore, the purified product is purified by dye terminator method (ABI PRISM Dye terminator Cycle Sequencing Ready
The reaction was determined from both directions using a reaction kit (trade name; manufactured by Perkin Elmer) and a 377 auto sequencer (trade name; manufactured by Perkin Elmer). As a result, Table 13
76.3-93.2% for HGV, GBV-C
And 70.3 to 80.5%, indicating high homology.
Therefore, the specificity was also considered to be high.

[0037]

[Table 2]

[Table 13]

Example 3 Positive Rate in Each Country and Region For 686 cases of liver disease, primer sets A1, 2
Thus, hepatitis G virus was detected in the same manner as in Example 1. The average positive rate was 20% (Table 3).

[0039]

[Table 3]

Example 4 Primer sets A1, 2 in 229 Mongolians
The detection was performed in the same manner as in Example 1. The detection results are as shown in Table 4. The detection rate was highest in cirrhosis. Also,
It was not detected in healthy subjects. Therefore, it became clear that the detection rate of HGV / GBV-C increases as the disease progresses. As shown in these examples, the GBV-C / HGV RNA detection method of the present invention has a higher detection rate than the conventional method and is considered to be useful for prognosis and prevention of hepatitis. In addition, it was found that superinfection with HCV was higher than HBV.

[0041]

[Table 4]

Example 5 Example of detection in 215 Chinese. The detection method is the same as in Example 1. The results are shown in Table 5. High detection rate with IVDU 61
% Were all HCV antibody-positive. On the other hand, it was not detected in donors, and showed a relatively high positive rate for liver cancer.
By using the primer sets A1 and A2 of the present invention, HGV / GBV-C can be actually detected.

[0043]

[Table 5]

Example 6 Quantification of HGV / GBV-C The amount of HGV / GBV-C RNA was quantified using the competitive-PCR (CRT-PCR) method.

Method: 1) Preparation of competitor Using primers 5gr4sp6 and 5gf3T7, pGEN-3Zf
(+) Vector (brand name; Promega) positions 3 to 86
And 152 bp was amplified by PCR. The amplification product was purified using a quick spin column G50 (TE) (trade name; manufactured by Boehringer Mannheim). The number of copies / μl was calculated from the OD 260 nm.

2) Adjustment of reference RNA. RNA extraction and cDNA synthesis were performed from the serum of a Japanese patient with liver disease in the same manner as in Example 1, and the first PCR was performed with the primer set B1 and the second PCR was performed with the primer set B2. The 183 bp amplification product was converted into a pGENT vector. It was cloned to obtain pMM3456. pMM3456, 1
0 μl was digested with 100 units of NotI (trade name; Boehringer Mannheim) at 37 ° C. for 3 hours, extracted with phenol / chloroform, and purified with isopropanol. This was dissolved in 5 μl of DEPC-treated water, and T7 RNA polymerase was added in the presence of RNase inhibitor.
RNA of HGV / GBV-C cDNA cloned with rase was synthesized. Further, the RNA obtained with RNAzolB was purified. The number of copies / μl was calculated from the OD260nm of the purified product.

3) 1 μl of the previously prepared reference RNA having a different concentration by 10 times and competitor 1 having a different concentration by 10 times.
μl was amplified with primer set B2. FIG. 1 shows an example of an electrophoresis pattern. The amounts of competitors in the tubes that showed the same level of amplification as the reference RNA matched.

In the same manner as in Example 2, the serum from Japanese chronic hepatitis patients to which interferon alpha (IFN) was administered
NA was extracted and cDNA synthesis was performed. The obtained cDN
1 μl of A was prepared from 1,000 to 1,000,000
Add up to 10 copies of different concentrations of competitors up to 00 copy in separate tubes, and add primer set B to P.
Gene amplification was performed with CR. PCR conditions are 96 ° C, 20
Second, 55 ° C., 20 seconds, 72 ° C., 20 seconds, 50 times. After the amplification, as shown in FIG. 1, the amount of competitor in a tube showing the same level of amplification as the cDNA derived from the patient's serum in the electrophoresis pattern was determined as the amount of HGV / GBV-C RNA of the patient. Similarly, 1 μl of the patient cDNA was subjected to the first PCR with each primer set B1 and the second PCR with primer set B2 to confirm the presence of HGV / GBV-C RNA. DNA
An 183 bp amplified band was detected with reference to the size marker.

As shown in FIG. 2, HGV /
GBV-C RNA amount was the 10 ⁶ copy / ml, rapidly become negative after IFN administration, after completion of administration, again, HCV-R
After a rise in NA, the concentration increased to 10 ^6.5 copy / ml, and became positive continuously thereafter, which coincided with the change in GPT. This means that the present invention makes it possible to detect the superinfection of HCV and HGV / GBV-C, and to quantify the RNA independently.
The present invention was found to be useful for the involvement, treatment and prevention of HGV / GBV-C RNA.

Example 7 Preparation of a competitor and preparation of a reference RNA were carried out in the same manner as in Example 6. 10 times different density created earlier by 10 times
0 to 100,000,000 copies of the reference RNA and 100
A solution containing 00 copies of the competitor and RNA prepared from human serum as in Example 1
Amplified by PCR in the same manner as in Example 1 with 3B and 5gr4AD. Furthermore, 0.05N Na was added to 5 μl of the PCR amplification product.
5 μl of OH was added and stirred. Furthermore, 0.5 M Tr
is-HCl (pH 6.8), 0.5 M NaCl,
0.1% SDS, 20% polyethylene glycol 80
00, 50 ng / ml 5'-end digoxigenin-labeled probe (PG
M3Dig: 5'-AAGCTTGCATGCCTGCAGGTCGACTCTAGA-3 ';5'
-Digoxigenin label) and stirred. 3
After leaving at 7 ° C. for 30 minutes, 100 μl thereof was transferred to a 96-well microplate (Nunc, trade name: Maxorp) on which biotin was adsorbed, and left to stand for 30 minutes. further,
PCR ELISA kit (Boehringer Mannheim,
405nm (reference)
nce filter 492 nm). As a negative control, DEPC-treated water was used for the same measurement, and the measured value was set to 0. The quantitative value of the sample was calculated from the measured value of the reference RNA.

Based on the quantitative values, classification was made into types 1, 2 and 3. Table 12 below shows the classification by region.

[0052]

[Table 12]

HGV / G of each country and by genotype
The quantitative value of BV-C RNA is shown in FIG. In each country, the average quantified value of HGV / GBV-C RNA is 10
⁶ became the vicinity of copies / ml, in Africa genotype ^1, at ^{10 4.5} relatively high rather than following cases copies / ml, also Japanese, in genotype 2 such Mongolia, 100 copies / ml There were cases showing a low value of.

Example 8 HGV / GBV-C RNA was detected with the primer sets B1 and 2 from the sera of 34 patients treated with IFN in Japanese patients with chronic hepatitis. Three cases were positive. Before IFN administration in all three cases,
After termination, HCV and HGV / GBV-C RNA were positive. In Case 1, both HCV and HGV / GBV-C RNA were positive even at the end of re-administration. Therefore, it was predicted that co-infection with HCV and HGV / GBV-C RNA would show resistance to IFN treatment. Therefore, if HGV / GBV-C RNA is detected, it can be predicted that it will show resistance to IFN treatment, and the present invention relates to the involvement of HGV / GBV-C RNA in co-infection with HCV hepatitis, its treatment and prevention. The present invention was found to be useful.

[0055]

[Table 6]

Example 9 Primer Sets A, B, C
Comparison of detection rates. RNA extraction and cDNA synthesis were performed in the same manner as in Example 1. The PCR conditions for the primer sets A1 and 2 were the same as in Example 1. Primer set B1,2, Primer set B3,4, Primer set C1,2 are the first PC
R was performed 35 times at 94 ° C, 20 seconds, 50 ° C, 20 seconds, 72 ° C, 20 seconds. The second PCR was performed at 94 ° C for 20 seconds and 5 seconds.
The test was performed at 5 ° C., 45 seconds, 72 ° C., 45 seconds, 35 times. As described below, the primer sets B1 and B2 had the highest detection rates.

[0057]

[Table 7]

Example 10 Serum HGV / GBV-C RNA was detected in Japanese and each chronic disease. HGV / GBV-C RNA was detected in a total of 209 patients with chronic liver disease, 197 patients with normal liver function and 12 patients with other diseases. The method was performed in the same manner as in Example 8, and the primer sets A1, 2, B1,
Positive when the amplified band of any of the primer sets was detected in the combination of 2, C1, and 2

As shown in Tables 8 and 9, HGV / GBV-CRNA was detected at a relatively higher rate in liver cirrhosis type C and liver cancer than in type B. In addition, it was not detected in those with normal liver function.

[0060]

[Table 8]

[0061]

[Table 9]

Example 11 The detection rates of the primer sets A1, 2 and B1, 2 were compared. Chinese, serum HGV / GBV-C RNA in each chronic disease
Was detected. 80 patients with chronic liver disease and IVDU
HGV / GBV- for 85 cases and 50 donors, for a total of 215 cases
C RNA was detected. The method was performed in the same manner as in Example 8, and a positive result was obtained when an amplified band was detected with the combination of the primer sets A1, 2 or B1, 2.

The extraction of RNA and the synthesis of cDNA were carried out in the same manner as in Example 2. 1) Primer sets A1 and 2 were performed in the same manner as in Example 2. 2) The primer sets B1 and B2 were used as follows.

The extraction of RNA and the synthesis of cDNA were carried out in the same manner as in Example 8. In the first PCR, primer set B1 was used at 94 ° C., 20 seconds, 50 ° C., 20 seconds, 72 ° C., 20 ° C.
Conducted 35 times per second. The second PCR was performed using primer set B2 at 94 ° C for 20 seconds, 55 ° C for 45 seconds, and 72 ° C.
C., 45 seconds, 35 times. As in Example 2, agarose gel electrophoresis compared the DNA size marker to 1
A specific band of 76 bp (177 bp) was confirmed.

As shown in Tables 10 and 11, all primer sets B1 and B2 had a high detection rate. In addition, since HGV / GBV-C RNA positive cases were detected from the donor with the primer sets B1 and B2, the donor can be used for screening. Thus, in particular, primer B
1 and 2 were found to be effective in preventing HGV / GBV-C.

[0066]

[Table 10]

[0067]

[Table 11]

Example 12 Classification by PCR-RFLP Method 1) Purification of RNA 100 μl of serum was subjected to RNAzolB (trade name, Biote)
x Lab) 900 μl, chloroform 150 μl
After stirring for 15 seconds in a 2 ml Eppendorf tube, the mixture was left at 4 ° C. for 5 minutes. 12000 rpm,
After centrifugation at 4 ° C. for 15 minutes, the supernatant was transferred to a 1.5 ml Eppendorf tube. Add isopropanol to this
After adding 50 μl and stirring, leave it for 15 minutes,
Centrifugation was performed at 4 ° C for 20 minutes to obtain a precipitate. 1 ml
After adding 75% ethanol and stirring, 12,000 rpm, 4
After centrifugation at ゜ C for 5 minutes, the supernatant was discarded. Repeat this operation for 2 more
After repeating twice, the resulting precipitate was dried.

2) cDNA synthesis A 5 × RT buffer (trade name; Life) was added to the purified RNA.
e Technologies, Inc. 2 μl,
0.1 μM DTT 1 μl, 10 mM dNTP 1
μl, Random Primer (trade name; Life
e Technologies Inc) 0.1 μl,
MMLV-reverse transcriptase (trade name; Life Techn)
logos Inc. ) 50 units, ribonuclease inhibitor (trade name; Takara Shuzo) 20 units
After the addition of ITS and DEPC-treated water, the mixture was stirred at a total of 10 μl and incubated at 37 ° C. for 1 hour.

3) PCR In order to increase the detection sensitivity, in the first PCR, primers 5gf3 and 5gr6 were used to convert the above cDNA into PC.
R amplification (Saiki et al., Science 239, 4)
81-491, 1988). Reaction solution is 10 mM
Tris-HCl (pH 8.3), 1.5 mM M
gCl2, 50 mM KCl, 800 μM dNTP,
1.25 units of AmpliTaq DNA poly
The test was carried out with a melase (trade name; Japan ROCHE).
PCR conditions were cycle 35, denaturation 94 ° C, 20 seconds,
Annealing was performed at 50 ° C. for 45 seconds and elongation at 72 ° C. for 45 seconds. Further, the amplified product was used for primer set 5 gf
A second PCR amplification was performed with 3, 5 gr4AD. PC
R conditions were a cycle 35, denaturation at 94 ° C. for 20 seconds, annealing at 55 ° C. for 45 seconds, and elongation at 72 ° C. for 60 seconds.

4) Cleavage of Restriction Enzymes 5 μl of the amplification product obtained in 3) was separately treated with each of the following restriction enzymes for 10 hours using 10 units. Restriction enzyme treatment temperature Treatment solution BsmF1 65 ゜ C NEBuffer 4 BsmAI 55 ゜ C NEBuffer BsmAI ScrFI 37 ゜ C NEBuffer 4 (trade name; manufactured by New England BioLabs)

5) Electrophoresis The amplification product was electrophoresed on agarose (tris borate buffer, 0.5 μg / ml ethidium bromide) (1
50V constant current). After electrophoresis, under ultraviolet irradiation,
Polaroid (Polaroid type 667) photography was performed.
The amplification product of 5 gf3, 5 gr4AD
In the case where the restriction enzyme treatment was not performed with reference to the NA size marker, an amplified band of about 183 bp was confirmed. The electrophoresis patterns of those treated with the restriction enzymes are shown in FIGS.

1) Determination of the base sequence of 5′UTR of HGV / GBV-C RNA. As in Example 1, RNA was extracted from serum and cDNA was extracted.
Synthesis was performed. The amplification product using the primers 5gf3 and 5gr4AD was converted into a quick spin column G50 (TE).
(Trade name; manufactured by Boehringer Mannheim). Furthermore, the purified product is dye terminato
r method (ABI PRISM Dye terminat
or Cycle Sequencing Ready
reaction kit (trade name; manufactured by PerkinElmer) using a 377 auto sequencer (trade name;
(PerkinElmer) from both directions.

The results for each region are shown in Table 16 below.

Example 13 HGV / GBV-C NS
Genotyping of 5B region 1) Purification of RNA 100 μl of serum was added to RNAzolB (trade name, Biote)
x Lab) 900 μl, chloroform 150 μl
After stirring for 15 seconds in a 2 ml Eppendorf tube, the mixture was left at 4 ° C. for 5 minutes. 12000 rpm, 4
The mixture was centrifuged at 15 ° C. for 15 minutes, and the supernatant was transferred to a 1.5 ml Eppendorf tube. Add isopropanol to 650
Add 15 μl and stir for 15 minutes after stirring.
Centrifugation was performed at 20 ° C. for 20 minutes to obtain a precipitate. In addition, 1 ml of 7
After adding 5% ethanol and stirring, the mixture was centrifuged at 12,000 rpm and 4 ° C. for 5 minutes, and the supernatant was discarded. After repeating this operation twice more, the obtained precipitate was dried.

2) cDNA synthesis A 5 × RT buffer (trade name: Life) was added to the purified RNA.
e Technologies, Inc. ) 2 μl,
0.1 M DTT 1 μl, 10 mM dNTP 1 μ
l, Random Primer (trade name: Life)
Technologies, Inc. , O .; D.
260 nm) 0.1 μl, MMLV-reverse transcriptase (trade name: Life Technologies, I
nc. ) 50 units, 20 units of ribonuclease inhibitor (trade name: Takara Shuzo) and DEPC-treated water were added, stirred at a total of 10 µl, and incubated at 37 ° C for 1 hour.

3) PCR In order to increase the detection sensitivity, in the first PCR, primers MG5BF3 and MUNS5R1
NA by PCR amplification (Saiki et al., Science
239, 481-491, 1988). The reaction solution was 10 mM Tris-HCl (pH 8.3).
5 mM MgCl ₂ , 50 mM KCl, 800 μM
dNTPs, 1.25 units of AmpliTaq DNA
Polymerase (Product name: Nippon ROCHE)
I went in. PCR conditions were cycle 35, denaturation 94 ° C,
The annealing was performed for 20 seconds, at 50 ° C. for 20 seconds, and at 72 ° C. for 20 seconds. Further, this amplified product is used as a primer MG5BF.
4. A second PCR amplification was performed with MUNS5R1. The PCR conditions were cycle 35, denaturation 94 ° C, 20
Second, annealing was performed at 55 ° C. for 20 seconds, and extension was performed at 72 ° C. for 20 seconds.

4) NS5 of HGV / GBV-C RNA
Determination of base sequence of B region The amplification product of 3) was converted to a quick spin column G50 (TE).
(Trade name: Boehringer Mannheim). Furthermore, the purified product is dye terminato
r method (ABI PRISM Dye terminat
or Cycle Sequencing Ready
reaction kit; trade name: manufactured by PerkinElmer) and determined from both directions using a 377 Auto Sequencer (tradename: manufactured by PerkinElmer).

5) Phylogenetic analysis Lasergene (trade name: DNA star)
The phylogenetic analysis was performed by the above-mentioned cluster methods. As a result, as shown in Table 15 and FIG.
There were three major categories worldwide.

[0080]

[Table 15]

Example 13 HGV / GBV-C RN
Detection of NS5A As in Example 1, R was determined from 10 sera of Japanese liver cancer patients.
After extracting NA and synthesizing cDNA, primers 5AF, 5AF
PCR was performed with ARH and 5ARG. The PCR conditions were 9
5 ° C, 15 seconds, 55 ° C, 45 seconds, 72 ° C, 45 seconds, 55
I went in times. The PCR product was detected in the same manner as in Example 7 using the probe 5APD (the 5 'end of SEQ ID NO: 359 labeled with digoxigenin). The absorbance at 405 nm of 0.5 or less was regarded as negative, and the absorbance at 405 nm or more was regarded as positive. In addition, detection was similarly performed using the primer sets A1 and 2. As a result of detection, the positive rate with primer set A1, 2 was 20% (2/10), and the detection rate with NS5A was 10%.
The detection rate was rather low (1/10).

Example 14 Detection of 5 ′ untranslated region using genotype-specific probe As in Example 1, RNA of 20 cases of GBV-C / HGV whose genotype was known by sequence was extracted and cDNA was synthesized. After that, primers 5gf3B and 5gr4
Perform PCR with AD and add 0.05 μl to 1 μl of PCR amplification product.
5 μl of N NaOH was added and stirred. Further 0.5
M Tris-HCl (pH 6.8), 0.5 M N
aCl, 0.1% SDS, 20% polyethylene glycol 8000, 50 ng / ml probe MUA (1
MUF (for detecting type 2; SEQ ID NO: 361) and MUAP (for detecting type 3; SEQ ID NO: 3)
62) Each 200 μl was added in a separate tube and stirred. After leaving at 37 ° C. for 30 minutes, 100 μl thereof was
A 96-well microplate (Nu
nc, trade name: Maxisorp) and left for 30 minutes. Further, the color developed by PCR ELISA (Boehringer Mannheim; trade name) was measured at 405 nm (ref) using an absorptiometer.
erence filter 492 nm). GBV-C / HGV typing was performed using each type of probe when the absorbance at 405 nm was 1 or more. As a result, as shown in Table 16 below, the results agreed with the sequence results. Therefore, GBV-C /
HGV type discrimination became possible.

[0083]

[Table 16]

[0084]

According to the present invention, a novel non-ABCCD
-Hepatitis EF virus subtype was found, its nucleotide sequence was determined, and means for its detection and classification were provided for the first time. Therefore, the present invention is expected to greatly contribute to the diagnosis of hepatitis.

[0085]

[Sequence list]

 SEQ ID NO: 1 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCCTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGTCTACCAGCGCCGCACCCTCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCACGAGCCCACGACTA

SEQ ID NO: 2 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGGAGGCTAA GATTCCACTT GTGCCTGTGG CGAGACAGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGGTGTCGACGACGAGGCCGACGACGAGGCCGACCAGCCGAGGCCGAGGCCGAGGGG

SEQ ID NO: 3 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TTTTGGTAGC CACTATAGGT GGGTTTTAAG 60 GGAAGGTTAA GATTCCTCTT GTGCCTGTGG CGAGACAGCG CACGGTCCGC AGGTGTTGGC TCCCAGGGTCGATCGAGGACTCAGGGCCTCAGACGAGTGTTGGCCCCCAGGGCTCGACCC

SEQ ID NO: 4 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TTTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGAAGGTTAA GATTCCTCTT GTGCCTGTGG CGAGACAGCG CACGGTCCAC AGGTGTTGGC 180 CCTACCGGTGCACGACGAGGGTCACGACGAGGGTCACGACGAGGTCGACGACGAGGTCGACGACGAGGTCGACGACGAGGCGCACGACGAGGCGCACGACGAGGCGCACGACGAGGCGCACGACGAGGTCGACGACGAGGCGCACGACGAGGGTCACGAGGACGACGAGGGTCACGAGCCGAGCAGGGCCGACCGAGGCGCACGAGGACGACGAGGCGCACGACGAGGTCGACGACGAGGCATCGACCGAGGCATCGACCGAGGCGCCGACGAGGACGACGAGGCGCACGAGGACGACGAGGCGGACGACGAGGGATCGACCAGGGCATCGAGCGAGGCAGA

SEQ ID NO: 5 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCCTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA TATTCCTCTT GTGCATGCGG AGAAAACGCG CACGGTCCAC AGGTGTCCGCCCCCTGCCGATCAGCCCGCCTGCGACGCCGCCTGCGACGCCGCCTGCGACCGCCCGCGTCGACGACCGCGCGACCGCGCGCGCGACCGAC

SEQ ID NO: 6 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCCTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGTGTGCGG CGAGAACGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGGTCGACGACCGCGTCGACGACCGCGTCGACGACCGCGTCGACGACCCGT

SEQ ID NO: 7 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCCTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACGCCGATGCGACTCGACCGACGCCCGTGCCGATGACGAGGCCGACGCGCCGACGCGCGCGCGCCGACGCGCCGAGGCCGACGCGCGCGCCGACGCGCCGACGCGCCGACGCGCGCCGACGCGCCGACGCGCGCGCCGACGCC

SEQ ID NO: 8 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCCTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTTTT GTGCCTGCGG CGAGACCGAG CACGGTCCAC AGGTGTTGAC 120 CCTACCCGGCCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGAGGACGAGTCAGCGGTCGATCGAGGACGAGGTCGATCGAGGACGAGGTCGAGGAG

SEQ ID NO: 9 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGAC 120 CCTACGCCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCCAGGTCGATCGATCGATCGATCGATCGATCGATCCAGGTCGATCGACGATCGTCCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCCATGCCGATCCATGCC

SEQ ID NO: 10 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTAAAG 60 AGAAGGTTAA CATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGCTGGC 120 CTTACCGCGACGACCGATGCGACTCAGCCGATGCGACTGACGACGCCCGTGCGACGACGACCGGG

SEQ ID NO: 11 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTAAAG 60 AGAAGGTTAA CATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACGCCGATGCGACCGACCGCTCGACGACGCCCGTGCGACGACGCCCAGG

SEQ ID NO: 12 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTAAAG 60 AGAAGGTTAA CATTCCTCTT GTGCCTGCGG CGAAACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACGCCGATGCGACGACGCCGATGCGACCGACCGCGCCGACGCCGACGCGCGCGACCGCGCCGACGCCGACGCGCGCCGACGCGCGCCGACGCCGAGGCCGACGACCGCGCCGACGCGCGCGCGCGCCGACGCCGAGGCCGACGCACGCGCCGACGCCCGTGCGACGACGCC

SEQ ID NO: 13 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTAAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCGC AGGTGTTGGC 120 CCTACCGCGACGACGCCGATGCGACCGACCGCGTCGACGACCGCCCGTGCGACGACGCCCGTGCGACGCAGCCCGTGCGACGCAGCCCGTGCGACGCAGCCCGTGCGACCGACCGCGCGACCGCGCCGAGGCGCACGCGCCGAGGCCGACGACCGCC

SEQ ID NO: 14 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACGCCGATGCGACGACGCCGATGCGACCGACCGTGCGACGACGCCCGTGCGACGACGCCCGTGCGACGACGCCCGTGCGACGACC

SEQ ID NO: 15 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA CATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACCGCGTCGATCGAC CCTGCGTACGAC CCCGTG TACTACGAC CCGTGGTCGACGACCGGG

SEQ ID NO: 16 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACCGTGCGACTCAGCCGATGCGACCGAGCCCGTGCGACGACGCCCGTGCGACGACGCCCGTGCGACCAGG

SEQ ID NO: 17 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACGCCGATGCGACTCAGCCGACGCGCCGACGCCCGTGCGACGCAGCCCGTGCGACGACGCCCGTGCGACGACGCCCGTGCGACCAGG

SEQ ID NO: 18 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCTT GTGCCTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACGCGTCGATCGACCGTGCGACTCAGCCGATGCGACCGACCGGTCGACCGACCGTGCGACCGACCGGTCGACCGACCGTGCGACCGACCGTGCGACCGACCGGT

SEQ ID NO: 19 Sequence length: 208 Sequence type: Nucleic acid sequence ACAGGGTTGG TAGGTCGTAA ATCCCGGTCA CCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 AGAAGGTTAA GATTCCTCCT GTGTTTGCGG CGAGACCGCG CACGGTCCAC AGGTGTTGGC 120 CCTACCGCGACGACCGTGCGACTCAGCCGATGCGACCGACCGGTCGACCGACCGTGCGACGCAGCCCGTGCGACGACGCCCGTGCGACGCAGCCCGTGCGACCAGG

SEQ ID NO: 20 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCCTAA ATCCCGGTCA TCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGTTGGTTAA GGTCCCTCTG GCGCTTGTGG CGAAAAAGGCG CACGGTTCGCAGGTGTTGGCTCCCAGAGGTGGTCGATCGAGGTCGATCGAGGTCGATCGAGGTCGATCGAGGTCGATCGAGGTCGATCGAGGTCGATCGAGGTCGATCGAGGCGCC

SEQ ID NO: 21 Sequence length: 208 Sequence type: Nucleic acid sequence CTAGGGTTGG TAGGTCCTAA ATCCCGGTCA TCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGTTGGTCAA CGTCCCTCTG GCGCTTGTGG CGAAAAAGGCGCACGGTCCCAGCGTGCTGCCGATCGAGGTCGATCGAGGAGCCACAGGTGTTGGCGCCCAGGGGTCAGGCCCC

SEQ ID NO: 22 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGTTGGTCAA GGTCCCTCTG GCGCTTGTGG CGAGAAAGCG CACGGTCCCAGCGTGCGGTCGACGACGAGGGTGCGGTCGACGACGAGGGTCGGCTCCCAGGGCCTCGAGGAGCCCC

SEQ ID NO: 23 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TTTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGTTGGTCAA GGTCCCTCTG GCGCTTGTGG CGAGAAAGCG CACGGTTCGACGACTC AGGTGTGGTCGATCGAC AGGTGTGGTCGATCCCAGGTGGTCGCCCAGAGGTGGTCACCC

SEQ ID NO: 24 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TTTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGGAGGTCAA GGTCCCTCTG GCGCTTGTGG CGAGAAAGCG CACGGTTCCAGCAG AGGTGTCAGGACGATCAGGGTCGATCGAC AGGTGTGGTCCCCC AGGTGTGGCATCCC

[0109] SEQ ID NO: 25 Length of sequence: 176 SEQ type: nucleic acid sequence TTGGTAGCCA CTATAGGTGG GTCTTAAGGG TTGGTTAAGG TCCCTCTGGC GCTTGTGGCG 60 AGAAAGCGCA CGGTCCACAG GTGTTGGCCC TACCGGTGTG AATAAGGGCC CGACGTCAGG 120 CTCGTCGTTA AACCGAGCCC AATACCCACC TGGGCAAACA ACGCCCACGT ACGGTC 176

SEQ ID NO: 26 Sequence length: 152 Sequence type: Nucleic acid sequence TAAGGGTTGG TTAAGGTCCC TCTGGCGCTT GTGGCGAGAA AGCGCACGGT CCACAGGTGT 60 TGGCCCTACC GGTGTGAATA AGGGCCCGAC GTCAGGCTCG TCGTTAAACC GAGCCCACTA 120 CCCACCTGGG CAAACAACTCACCGAC

SEQ ID NO: 27 Sequence length: 154 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTTAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCAA CCGGTGTGAA TAAGGACCCG ACGTCGGGCT CGTCGTTAAA CCGAGCCCAC 120 TACCCACCTGGGCACACA

SEQ ID NO: 28 Sequence length: 154 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCTGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGCACAAC

SEQ ID NO: 29 Sequence length: 154 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTTAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGGT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCGGTCCAGGACCA

SEQ ID NO: 30 Sequence length: 153 Sequence type: Nucleic acid sequence TTAAGAGAAG GTTAAGATTC CTCTTGTGCC TGCGGCGAGA CCGCGCACGG TCCACAGGTG 60 TTGGCCCTAC CGGTGCTAAT AAGGGCCCGA CGTCAGGCTC GTCGTTAAAC TGAGCCCGTCCGACTACGCGATCGCACGA

SEQ ID NO: 31 Sequence length: 176 Sequence type: Nucleic acid sequence TTGGTAGCCA CTATAGGTGG GTCTTAAGGG TTGGTTAAGG TCCCTCTGGC GCTTGTGGCG 60 AGAAAGCGCA CGGTCCACAG GTGTTGGCCC TACCGGTGTG AATAAGGGCC CGACGTCAGG 120 CTCGTCGTACACCACGGCC

[0116] SEQ ID NO: 32 Length of sequence: 176 SEQ type: nucleic acid sequence TTGGTAGCCA CTATAGGTGG GTCTTAAGGG TTGGTTAAGG TCCCTCTGGC GCTTGTGGCG 60 AGAAAGCGCA CGGTCCACAG GTGTTGGCCC TACCGGTGTG AATAAGGGCC CGACGTCAGG 120 CTCGTCGTTA AACCGAGCCC AATACCCACC TGGGCAAACA ACGCCCACGT ACGGTC 176

SEQ ID NO: 33 Sequence length: 152 Sequence type: Nucleic acid sequence TAAGGGTTGG TTAAGGTCCC TCTGGCGCTT GTGGCGAGAA AGCGCACGGT CCACAGGTGT 60 TGGCCCTACC GGTGTGAATA AGGGCCCGAC GTCAGGCTCG TCGTTAAACC GAGCCCACTA 120 CCCACCTGGG CAAACAACGCGACGACAA

SEQ ID NO: 34 Sequence length: 154 Sequence type: Nucleic acid sequence TTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGCCGCGCACG

SEQ ID NO: 35 Sequence length: 204 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT ATAGGTGGGT CTTAAGGGTT 60 GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACAGT GTCCACTA 180 CCGGTGAATCAGCCGACGCGACCGCGACCGACCGCGACCGCGACCGCGACCGCGACCGCGACCGCGCGACCGCGCGACCGCGCGCGACGGCGCGCGACGGCGCGCGACGGCCGGGG

[0120] SEQ ID NO: 36 SEQ Length: 203 Type of sequence: nucleic acid sequence GTTGGTAGGT CGTAAATCCC GGTCATCTTG GTAGCCACTA TAGGTGGGTC TTAAGGGTTG 60 GTCAAGGTCC CTCTGGCGCT TGTGGCGAGA AAGCGCACGG TCCACAGGTG TTGGCCCTAC 120 CGGTGTGAAT AAGGGCCCGA CGTCAGGCTC GTCGTTAAAC CGAGCCCATT ACCCACCTGG 180 GCAAACGACG CCCACGTACG GTC 203

SEQ ID NO: 37 Sequence length: 208 Sequence type: Nucleic acid sequence CCAGGGTTGG TAGGTCGTAA ATCCCGGTCA TCTTGGTAGC CACTATAGGT GGGTCTTAAG 60 GGTTGGTCAA GGTCCCTCTG GCGCTTGTGG CGAGAAAGCG CACGGTCCGC GGGTCGCGTCGACGACGAGGGTCGGCTCGAC AGGTGTTGGC 180

SEQ ID NO: 38 Sequence length: 212 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT ATAGGTGGGT CTTAAGAGAA 60 GGTTAAGATT CGTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT GTCGCCGCGCGCTCGCCCCACGCCCAGCGTCATAGGGCCCGCCTACGCCCAGCGTCATAGGGCCCC

SEQ ID NO: 39 Sequence length: 214 Sequence type: Nucleic acid sequence TGGTTGGTAG GTCGTAAATC CCGGTCACCT TGGTAGCCAC TATAGGTGGG CTTTAAGAGG 60 AGGTTAAGAG TCCGCTTACG CCTGGGGCGA GACCGCGCAC GGTCCACAGG TGCACGCACGCCGATCGATCGACGCCGATCGATCGACGCCGATCGATCGACGCCGATCATACGCACGCCGATCATAGCGGCCGATCATACGCACGCCGATCATAGCGGCCGATCATAGCGGCCGCGATCATAGCGGCCGATCATACGCACGCCGATCGATCCACGCCGATCGATCGACGACGATCATACGCGCCGCGA

SEQ ID NO: 40 Sequence length: 212 Sequence type: Nucleic acid sequence GGCTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT ATAGGTGGGT CTTAAGAGAA 60 GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT GTTGGCGC GCTCGATCAGTCCGTCGATCAGTCCGTCGATCAGTCCGTCCAGCAGTCAGTCGATCAGTCCGTCCAGCAGTCAGTCGATCAGTCGATCAGCTCGACCAGGCGCCTCGATCAGCTCGTC

SEQ ID NO: 41 Sequence length: 212 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACC ATAGGTGGGT CTTAAGGGTT 60 GGTCAAGGTC CCTTTGGCGC TTGGGGCGAG AAAGCGCGCACG GTCCACCGGT GTTGGCCCTA 120 CCGGTGTGAATCAGCCGACGCGCGACCGCGACCGCGACCGCGCGCGACCGCGCGCGACCGCGCGCGACCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCG at collection at

SEQ ID NO: 42 Sequence length: 213 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT ATAGGTGGGT CTTAAGGGTT 60 GGTCAAGGTC CCTCTGGCGC TTGTGGCGAGTCAACGCGCACG GTCCACCA CGCTCAGCAGCCGTCGTGCGACTCAGCCAGCCGTCGGCACAGCCGTCGTGCAGCAGCCGTCC

SEQ ID NO: 43 Sequence length: 212 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT ATAGGTGGGT CTTAAGAGAA 60 GGTTAAGATT CCTCTTGTGC CTGGGGCGAG ACCGCGCACG GTCCACAGGT GTTGGCGC GCCCTA 120 CCGGTGCCGCGCGTCA TAAGGGCCCGTCGCGTCA TAGGGGCCCAGCGTCAGG

SEQ ID NO: 44 Sequence length: 212 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT ATAGGTGGGT CTTAAGAGAA 60 GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT GTTGGCGC GCCCTA 120 CCGGTGCACGACGCAGCAGCAGCAGCAGGCAGCAGCAGCCAGCAGCAGGCAGCAGG

SEQ ID NO: 45 Sequence length: 212 Sequence type: Nucleic acid sequence GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT ATAGGTGGGT CTTAAGAGAA 60 GGTTAAGATT CCTCTTGTGC ATGCGGCGAG AACGCGCACG GTCCACAGGT GTTGGCCCTA 120 CCGGTGCATCGCCCTGCCAAGG

SEQ ID NO: 46 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGGATG CGAACCGGAA GGCATCTCGT TTTCTGCCAC TCCAAGGCTG AGTGCGAGCG 60 CCTTGCTGGC CAGTTTTCCG CTAGGGGAGT TAATGCCATC GCCTATTATA GGGGCAAA 118

SEQ ID NO: 47 Sequence length: 118 Sequence type: nucleic acid sequence TGAGCGGATG CGCACCGGGA GGCACCTTGT ATTYTGCCAT TCAAAGGCTG AGTGCGAGCG 60 CCTTGCTGGC CAGTTCTCCG CTAGGGGAGT GAACGCCATC GCCTATTACA GGGGTAAG 118

SEQ ID NO: 48 Sequence length: 118 Sequence type: nucleic acid sequence TGAGCGGATG CGCACCGGTA GGCATCTCGT GTTCTGCCAT TCCAAGGCGG AGTGCGAACG 60 CCTGGCCGGC CAGTTCTCCG CCAGGGGGGT CAATGCCATC GCCTATTATA GGGGCAAG 118

SEQ ID NO: 49 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGAGGATG CGGACCGGCA GGCATCTCAT ATTTTGCCAC TCAAAGGCGG AGTGTGAACG 60 CTTGGCGGGC CAGTTCTCTT CCCGGGGAGT AAATGCCATT GCCTATTATC GGGGGAAG 118

SEQ ID NO: 50 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGAACCGGCA GGCATCTCGT GTTTTGCCAC TCAAAGGCGG AGTGCGAGCG 60 CTTGGCGGGC CAATTTTCCT CCAGGGGTGT CAATGCCATT GCATATTATA GGGGTAAG 118

Sequence length: 118 Sequence type: nucleic acid sequence SEQ ID NO: 51 GGAGCGGATG CGAACCGGCA GGCATCTCGT GTTTTGTCAC TCAAAGGCGG AGTGCGAGCG 60 CTTGGCGGGC CAATTTTCCT CCAGGGGTGT CAATGCCATT GCATATTATA GGGGTAAG 118

SEQ ID NO: 52 Sequence length: 118 Sequence type: nucleic acid sequence GGAGAGGATG CGGACCGGGA GGCATCTCGT CTTCTGCCAC TCAAAAGCGG AATGCGAGCG 60 CNTGGCGGGC CAATTCTCTT CCCGGGGGGT GAANGCCATN GCATATTATC GGGGAAAG 118

SEQ ID NO: 53 Sequence length: 118 Sequence type: nucleic acid sequence GGAGCGGATG CGTACTGGTA GGCACCTCGT GTTCTGCCAC TCAAAGGCGG AGTGTGAGCG 60 GCTTGCTGGT CAGTTTTCCT CTAGGGGGGT AAACGCCATT GCCTATTATA GGGGTAAG 118

SEQ ID NO: 54 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGTACTGGTA GGCACCTCGT GTTCTGCCAC TCAAAGGCGG AGTGTGAGCG 60 GCTTGCTGGT CAGTTTTCCT CTAGGGGGGT AAATGCCATT GCCTATTATA GGGGTAAG 118

SEQ ID NO: 55 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGGATG AGGACTGGCA GGCATCTCGT ATTCTGTCAC TCAAAGGCAG AGTGTGAGCG 60 TCTGGCGGGC CAATTCTCCT CCCGGGGGGT CAATGCTATT GCATACTACA GGGGTAAG 118

SEQ ID NO: 56 Sequence length: 118 Sequence type: nucleic acid sequence GGAGCGGATG CGGACCGGCA GGCATCTCGT ATTCTGTCAC TCGAAGGCAG AGTGTGAGCG 60 TCTGGCTGGT CAGTTTTCCT CTAGGGGGGT CAATGCCATC GCCTATTATC GGGGGAAG 118

SEQ ID NO: 57 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGGACCGGCA GGCACCTTGT ATTCTGCCAT TCCAAGGCGG AGTGCGAGCG 60 CTTGGCGGGC CAATTCTCCT CTAGGGGGGT AAACGCCATT GCCTATTACC GGGGGAAG 118

SEQ ID NO: 58 Sequence length: 118 Sequence type: nucleic acid sequence GGAGAGGATG CGGACCGGGA GGCATCTCGT CTTCTGCCAC TCAAAAGCGG AATGCGAGCG 60 CCTGGCGGGC CAATTCTCTT CCCGGGGGGT GAACGCCATC GCCTATTATC GGGGAAAG 118

SEQ ID NO: 59 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGAGGATG CGGACCGGGA GGCATCTCGT CTTCAGCCAC TCAAAGGCGG AATGCGAGCG 60 CTTAGCGGGC CAATTCTCTT CCCGGGGGGT GAACGCCATT GCCTATTATC GGGGAAAG 118

SEQ ID NO: 60 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGGACCGGTA GGCACCTCGT GTTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 TCTGGCTGGT CAGTTTTCCT CTAGGGGGGT CAATGCCATT GCCTATTATGGGGAAAG 118

SEQ ID NO: 61 Sequence length: 118 Sequence type: Nucleic acid sequence CGAACGGATG CGAACCGGCA GGCACCTCGT ATTCTGCCAT TCCAAGGCGG AGTGCGAGCG 60 GCTGGCGGGG CAGTTTTCGG CCCGGGGGGT TAATGCTATT GCTTATTATG AGGGAAAG 118

SEQ ID NO: 62 Sequence length: 118 Sequence type: nucleic acid sequence GGAACGGATG CGAACTGGTA GGCATCTCGT ATTCTGCCAT TCAAAAGCAG AGTGCGAACG 60 CCTGGCCGGT CAGTTTTCGT CTAAGGGTGT CAATGCTATT GCCTATTATC GGGGGAAA 118

SEQ ID NO: 63 Sequence length: 118 Sequence type: nucleic acid sequence GTAGCGGATG CGTACTGGTA GGCACCTCGT GTTCTGCCAC TCAAAGGCGG AGTGTGAGCG 60 GCTTGCTGGT CAGTTTTCCT CTAGGGGGGT AAATGCCATT GCCTATTATA GGGGTAAG 118

SEQ ID NO: 64 Sequence length: 118 Sequence type: nucleic acid sequence GGAGCGGATG CGGACCGGTA GGCATCTTGT ATTCTGCCAC TCAAAGGCGG AGTGCGAGCG 60 CCTGGCGGGC CAATTCTCTT CCAGGGGAGT CAATGCCATT GCCTATTATA GGGGCAAG 118

SEQ ID NO: 65 Sequence length: 118 Sequence type: Nucleic acid sequence CGAGCGGATG CGGACCGGGA GGCACCTCGT GTTCTGCCAT TCAAAGGCGG AGTGTGAGCG 60 CCTGGCGGGC CAGTTCTCTG CAAGGGGGGT GAACGCCATC GCGTATTACA GGGGTAAG 118

SEQ ID NO: 66 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGAACCGGCA GGCACCTCGT ATTCTGCCAC TCGAAGGCGG AGTGTGAGCG 60 GCTTGCTGGC CAGTTTTCCT CCAGGGGGGT CAACGCCATA GCCTATTGAT AGGGAAAG 118

SEQ ID NO: 67 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGGATG CGAACTGGGA GGCACCTCGT ATTCTGCCAC TCGAAGGCTG AGTGCGAGCG 60 CCTCGCCGGT CAGTTTTCCG CCAGGGGGGT TAATGCCATC GCCTATTATA GGGGCAAA 118

SEQ ID NO: 68 Sequence length: 118 Sequence type: nucleic acid sequence TGAGCGAATG CGGACCGGAA GGCACCTCGT TTTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 CCTCGCCGGT CAGTTTTCTG CCAGGGGGGT CAATGCCATT GATTATTACA GGGGCAAA 118

SEQ ID NO: 69 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGGATG CGGACTGGGA GGCACCTCGT ATTCTGCCAC TCGAAGGCTG AGTGCGAGCG 60 CCTCGCCGGT CAGTTTTCCG CCAGGGGGGT TAATGCCATC GCCTATTATA GGGGCAAA 118

SEQ ID NO: 70 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGGATG CGGACTGGGA GGCACCTCGT ATTCTGCCAC TCGAAGGCTG AGTGCGAGCG 60 TCTCGCCGGT CAGTTTTCCG CCAGGGGGGT TAATGCCATC GCCTATTATA GGGGCAAA 118

SEQ ID NO: 71 Sequence length: 118 Sequence type: nucleic acid sequence TGAGCGGATG CGAACCGGAA GGCACCTCGT ATTCTGCCAC TCAAAGGCGG AGTGTGAGCG 60 CCTCGCCGGC CAGTTCTCTG CAAGAGGGGT GAACGCCATT GCCTATTACA GGGGAAAG 118

SEQ ID NO: 72 Sequence length: 236 Sequence type: Nucleic acid sequence CGAGCGGATG CGAACCGGCA GGCACCTCGT ATTCTGCCAC TCTAAGGCGG AGTGTGAGCG 60 TCTTGCTGGG CAGTTCTCTG CAAGGGGGGT AAATGCCGGTCGCTATTATA GGGGAAGTCGATCGGTCAGTCGGTCTCGGTCAGTCGGTCTCGGTCGGTCGGTCTCGGCT

SEQ ID NO: 73 Sequence length: 118 Sequence type: nucleic acid sequence TGAGCGGATG CGAACCGGTA GGCATCTCGT ATTCTGCCAT TCTAAGGCGG AGTGCGAGAG 60 GCTAGCTGGC CAGTTTTCAG CTAGGGGGGT CAATGCCATT GCTTATTATA GGGGAAAG 118

SEQ ID NO: 74 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGAATG CGAACCGGTA GGCATCTCGT ATTCTGTCAC TCCAAGGCTG AGTGCGAGCG 60 CCTTGCCGGC CAGTTTTCCG CTAGGGGAGT CAACGCCATC GCCTTATACA AGGGAAAG 118

SEQ ID NO: 75 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGGATG CGGACTGGTA GGCATCTCGT TTTCTGCCAC TCCAAGGCGG AGTGCGAGCG 60 TCTCGCCGGT CAGTTTTCTG CCAGGGGGGT TAACGCCATT GCCTATTATA GGGGTAAG 118

SEQ ID NO: 76 Sequence length: 118 Sequence type: Nucleic acid sequence YGAGCGAATG CGGACCGGAA GACACCTCGT ATTCTGCCAC TCTAAGGCGG AGTGTGAGCG 60 ACTGGCTGGT CAGTTTTCTG CCAGGGGGGT GAATGCCATC GCCTATTACA GGGGCAAA 118

SEQ ID NO: 77 Sequence length: 118 Sequence type: nucleic acid sequence GGAGCGGATG CGGACTGGGA GGCATCTCGT ATTCTGCCAC TCAAAGGCTG AGTGCGAACG 60 CCTCGCGGGC CAATTCTCGG CCAGGGGTGT TAATGCTATC GCCTATTATA GGGGAAAA 118

SEQ ID NO: 78 Sequence length: 118 Sequence type: nucleic acid sequence GGAGAGGATG CGGACCGGGA GGCACCTCGT GTTCTGCCAT TCCAAGGCGG AATGTGAAAG 60 ACTCGCGGGC CAATTTTCTT CCAGGGGCGT CAATGCCATT GCCTATTACA GGGGAAAG 118

SEQ ID NO: 79 Sequence length: 118 Sequence type: nucleic acid sequence CGAGCGGATG CGTACTGGCA CGCACCTCGT ATTCTGCCAT TCCAAGGCGG AGTGCGAAAG 60 GTTAGCCGGC CAGTTCTCTG CCAGGGGGGT CAATGCCATC GCTTATTATA GGGGAAAG 118

SEQ ID NO: 80 Sequence length: 118 Sequence type: Nucleic acid sequence TGAGCGGATG CGGACCGGGA GGCACCTCGT ATTCTGCCAC TCCAAGGCTG AGTGTGAGCG 60 CCTTGCCGGC CAGTTTTCTG CTAGAGGGGT GAACGCCATT GCTTATTATA GGGGCAAA 118

SEQ ID NO: 81 Sequence length: 118 Sequence type: Nucleic acid sequence CGAGAGGATG CGGACCGGCA GGCATCTCGT ATTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 GCTGGCCGGA CAGTTCTCTG CCAGGGGGGT TAACGCCATC GCCTATTATA GGGGGAAG 118

SEQ ID NO: 82 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGAGGATG CGGACCGGCA GGCACCTCGT ATTCTGTCAC TCCAAGGCGG AGTGCGAGCG 60 CCTGGCGGGC CAGTTTTCTT CCCGGGGGGT GAATGCCATT GCCTACTACA GGGGAAAG 118

SEQ ID NO: 83 Sequence length: 118 Sequence type: nucleic acid sequence CGAGAGGATG CGGACTGGGA GGCATCTCGT ATTCTGCCAC TCAAAGGCGG AGTGCGAACG 60 CCTCGCGGGC CAGTTCTCGG CTAGGGGTGT CAATGCCATT GCCTATTATA GGGGGAAG 118

SEQ ID NO: 84 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGAGGATG CGGACTGGCC GGCACTTAGT ATTCTGCCAT TCAAAAGCGG AGTGCGAGCG 60 GCTGGCCGGC CAATTCTCGG CCAGGGGGGT CTATGCCATT GCTTATTACA GGGGGAAG 118

SEQ ID NO: 85 Sequence length: 118 Sequence type: Nucleic acid sequence CGAGCGGATG CGAACCGGGA GGCATCTCGT ATTCTGCCAC TCCAAGGCTG AGTGCGAGCG 60 CCTTGCCGGC CAGTTTTCCG CTAGGGGGGT CAATGCCATT GCCTATTATA GGGGCAAA 118

SEQ ID NO: 86 Sequence length: 118 Sequence type: Nucleic acid sequence GGAAAGGATG CGGACCGGGA GGCACCTCGT GTTCTGCCAC TCAAAGGCTG AGTGCGAGCG 60 CCTCGCGGGC CAGTTTTCTT CTAGGGGAGT CAATGCTATT GCCTATTACA GGGGAAAG 118

SEQ ID NO: 87 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGGACCGGGA GGCACCTCGT GTTCTGCCAC TCAAAGGCGG AGTGCGAGCG 60 CCTCGCGGGC CAATTCTCAG CCAGGGGGGT CAATGCCATT GCCTATTATA GGGGGAAA 118

SEQ ID NO: 88 Sequence length: 152 Sequence type: Nucleic acid sequence TGGGCATGGG ATNCCYCTGG AACGGATGCG AACTGGTAGG CATCTCGTAT TCTGCCATTC 60 AAAAGCAGAG TGCGAACGCC TGGCCGGTCA GTTTTCGTCT AAGGGTGTCA ATGCTATTGC 120 CTATTATCGGTCCATGA

SEQ ID NO: 89 Sequence length: 144 Sequence type: Nucleic acid Sequence CCYCTCGAAC GGATGCGAAC CGGCAGGCAC CTCGTATTCT GCCATTCCAA GGCGGAGTGC 60 GAGCGGCTGG CGGGGCAGTT TTCGGCCCGG GGGGTTAATG CTATTGCTTA TTATGAGGAG 120 C

SEQ ID NO: 90 Sequence length: 161 Sequence type: Nucleic acid Sequence TGGGCATGGG ATTCCYCTGG AGCGGATGCG GACCGGTAGG CACCTCGTGT TCTGCCATTC 60 AAAGGCGGAG TGCGAGCGTC TGGCTGGTCA GTTTTCCTCT AGGGGGGTCA ATGCCATTGC 120 CTATTATCGG GTCCATGAGAGAGA

SEQ ID NO: 91 Sequence length: 118 Sequence type: Nucleic acid Sequence AGAGCGGATG CGGACCGGGA GGCATCTCGT ATTCTGCCAC TCAAAGGCTG AGTGCGAGCG 60 TCTCGCGGGC CAATTCTCGG CTCGGGGGGT TAACGCCGTC GCCTATTATA GGGGTAAG 118

SEQ ID NO: 92 Sequence length: 148 Sequence type: Nucleic acid sequence TATGGGCMTG GGATTCCCCT TATGGGCATG GGATHCCYCT GGAGCGGATG CGGACCGGTA 60 GGCACCTCGT GTTCTGCCAT TCAAAGGCGG AGTGCGAGCG TCTGGCTGGT CAGTTTTCCT 120 CTAGGGGGCT CAATGCCATT GC

[0177] SEQ ID NO: 93 SEQ Length: 220 Type of sequence: nucleic acid sequence TGATCGAGTG GGACTCAAGA ACGTTTCCCT CTTGATTGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ACCCGAGTGA GGCCTTGCGC AGAGCCCTGG CTTCATATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTGT GCTCCATTTG 180 GTTAGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0178] SEQ ID NO: 94 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAAA ACGTGTCCCT TCTGATTGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTGTATGCG ACCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCATATGG 120 GTATGCGTGT GAGCCCTCAT ATCATGCATC ATTGGATACA GCTCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCTG ATGGGAAGCG CCATTTCTTC 220

[0179] SEQ ID NO: 95 SEQ Length: 220 Type of sequence: nucleic acid sequence GGAGCGGGTG GGACTCAAGA AAGTTTCCCT CCTGAACGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ATCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCTTATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACA GCTCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAATGCGG ATGGGAAGCG CCATTTCTTC 220

[0180] SEQ ID NO: 96 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAAA ACGTGTCCCT TCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ATCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTATGG 120 GTACGCGTGT GAGCCCTCAT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCAGTTG 180 GTTGGCCGAA TGTAATGCGG ACGGGAAGCG CCATTTCTTC 220

[0181] SEQ ID NO: 97 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAGA ACGTTTCCCT CCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CATCATACGG 120 GTATGCGTGT GAGCCCTCAT ATCATGCATC ATTGGACAAG GCCCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAACGCGG ATGGGAAGCG CCATTTCTTC 220

[0182] SEQ ID NO: 98 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCA GGAGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAGTGTGCG ACCCTAGCGA CGCTCTGGGC AGAGCCCTAG CAAGCTATGG 120 GTACGCGTGC GAGCCCTCAT ACCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0183] SEQ ID NO: 99 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTC GGACTCAAAA ACGTGTCCCT CCTAATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTACGG 120 GTATGCATGT GAGCCCTCAT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 100 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGCGGGTG GGACTCAAAA ACGTTTCCCT CCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCCTAGGCAGAGCCTTGG CTTCCTGCGTAT GAGCCTGCGTCCTCATGATGCTGCT GCATCCTGCT GCATCCTGCT GCATCCTGCCATCATGATGCGTCT GAGCCTGCCATCT

SEQ ID NO: 101 Sequence length: 220 Sequence type: Nucleic acid sequence CGAACGAGTG GGACTCAAAA ACGTTTCCCT CTTGATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CTTCATACGG 120 GTATGGCTCGATCGATCGATCGATCGATCGCATCGATGCGTCATGT GAGCCCC ATGTCATGCGTCAGT

[0186] SEQ ID NO: 102 SEQ Length: 220 Type of sequence: nucleic acid sequence TGATCGGGTG GGACTCAAAA ACGTGTCCCT TCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ATCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTATGG 120 GTACGCGTGT GAGCCCTCAT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GTTAGCCGAG TGTAATGCGG ACGGGAAGCG CCATTTCTTC 220

[0187] SEQ ID NO: 103 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTC GGACTCAAAA ACGTGTCCCT CCTAATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTACGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0188] SEQ ID NO: 104 Length of sequence: 220 SEQ type: nucleic acid sequence CGATCGGGTG GGACTCAAGA ACGTGTCCCT CCTGATCGCC GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGCGA GGCTTTGGGC AGAGCTCTTG CCTCGTACGG 120 GTATGCGTGT GAGCCCTCAT ATCATGCATC ACTGGACACA GCTCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGTAATGCTG ACGGGAAGCG TCATTTCTTC 220

SEQ ID NO: 105 Sequence length: 220 Sequence type: Nucleic acid sequence CGATCGGGTG GGACTCAAAA ACGTTTCCCT CCTGATCGCG GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCGATGTGCG ACCCGAGCGA GGCCCTGGTC AGAGCCCTGG CATCGTACGG 120 GTATGCATCTGATCGATCGATCGATCGATCTCATGT GAGCCCT ATGTCATGT GAGCCTAG ATGTCATGT GAGCCTC ATGTCATGT GAGCCTAG ATGTGAGCCT ATGTCATGCGTCAGT

[0190] SEQ ID NO: 106 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGTGA CGCGCTGGGC AGAGCCCTCG CGAGCTACGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GTTAGCTGAG TGTAATGCGG ATGGGAAGCG CCATTTCTTC 220

[0191] SEQ ID NO: 107 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ACGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGCG ATCCTAGTGA CGCGCTGGGC AGAGCCCTAG CTAGCTACGG 120 ATACGCGTGC GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 108 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGCGGGTG GGACTGAAAA ACGTTTCACT CCTAATCGCA GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCAATATGCG ACCCCAGTGA GGCCTTGGGC AGAGCCCTGG CGTCATATGG 120 GTACGCGTGATCGTCTGATCGTCTGATCGATCTGCTGATCGTCTGATCGTGCACCGTCATGCGTCATGCGTCATGCGTCATGCGTGATCCTGCGATCTGCTGATCGCTCGAG

SEQ ID NO: 109 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGAGGGTG GGACTGAAAA ACGTTTCACT CCTCATAGCA GGTGACGACT GTCTGATCAT 60 ATGTGAACGG CCGGTGTGCG ACCCTAGCGA AGCCTTGGGC AGAGCCCTGG CAAGCCGTCAGCT GAGCCTTCCTGCT GCATGCATCGATCGATCATGCATGCATGCCAGCATGCATGCCAGCATGAT

SEQ ID NO: 110 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGACTGAAAA ATGTCTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTTTGCG ACCCTAGCGA CGCCTTGGGC AGAGCCCTGG CAAGCTATGG 120 GTACGCTCGCATGATCGCTTCGATGCTTGC GATCGCGTGCTTGC GATCGCGTGCTTGC GATCGCTCGCATTC

[0195] SEQ ID NO: 111 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGACTGAAGA ACGTCTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAGAGG CCTATATGTG ACCCTTGCGA TGCCTTGGGC AGAGCCCTAG CTAGCTATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ACGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 112 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCC GGAGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGTG ATCCTAGCGA AGCCTTGGCC AGAGCCCTGG CAAGCTGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCAGCATGAT

[0197] SEQ ID NO: 113 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCGGTGTGCG ATCCTAGTGA CGCCCTGGGC AGAGCCCTGG CGAGCTACGG 120 ATACGCATGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCGG ATGGGAAACG CCATTTCTTC 220

SEQ ID NO: 114 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGAGTG GGACTGAAAA ATGTTTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCCAGCGA GGCTTTAGGC AGAGCCTTGG CTAGCTACGG 120 GTACGCTCGATCGATCGATCGCTGCAT GAGCCATCCTGC GATCCATGCT

[0199] SEQ ID NO: 115 SEQ Length: 220 Type of sequence: nucleic acid sequence TCAGAGGGTG GGACTGAAAA ATGTCTCATT GCTCATAGCA GGCGACGACT GTCTGATCAT 60 ATGTGAACGG CCAGTGTGCG ACCCTTGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTATGCATGC GAGCCTTCAT ATCATGCATC ATTGGACACG GCTCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCTG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 116 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATCGCA GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAGTATGCG ACCCTAGTGA CGCCNTGGGC AGAGCCCTGG CGAGCTACGG 120 GTATGCGCTGCAT GAGCCCTGCGATCGCATCATGAT CGCATCGATGCGTCC

SEQ ID NO: 117 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTATGCG ACCCTAGTGA TGCCTTGGGT AGAGCCTTGG CGAGCTATGG GCTCGATCGATCGATCGATCGATCGATCGATCGTCGATCGCATGCGTGCGCCC ATGTGTGCGACGTC

SEQ ID NO: 118 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGAGGGTG GGGCTGAAAA ATGTCTCCCT TCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTATGCG ACCCTTGCGA CGCATTGGCT AGAGCCCTCG CTAGTTATGG 120 GTATGCTCGCGATCGATCGCTGCT GATCTTGCTGCT GAGATCTCGCTGCAT GATCTTCCTGAT

[0203] SEQ ID NO: 119 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ACGTTTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA CGCCTTGGGC AGAGCCCTCG CTAGCTATGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 120 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAATGTGCG ACCCTAGTGA CGCTTTGGGC AGAGCCCTAG CGAGCTATGGTCGATCGATCCATGCCATCGTCGATCCGTCCATGCTCGATCGATCCATGCTGCCATCGTCATGCGCCCAT

[0205] SEQ ID NO: 121 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCGGTGTGCG ACCCGAGTGA CGCTTTGGGC AGAGCCCTAG CGAGCTATGG 120 GTACGCGTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCGG ATGGGAAACG CCATTTCTTC 220

[0206] SEQ ID NO: 122 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCTGTTTGCG ACCCTAGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTACGCTTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTTT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0207] SEQ ID NO: 123 SEQ Length: 220 Type of sequence: nucleic acid sequence TCAGAGGGTG GGACTGAAAA ATGTCTCATT GCTCATAGCA GGCGACGACT GTCTGATCAT 60 ATGTGAACGG CCAGTGTGCG ACCCTTGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTATGCATGC GAGCCTTCAT ATCATGCATC ATTGGACACG GCTCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCTG ACGGGAAACG CCATTTCTTC 220

[0208] SEQ ID NO: 124 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGCGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCT GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAATGTGTG ATCCTAGCGA GGCCTTGGGC AGAGCCCTTG CCAGCTACGG 120 GTACGCATGT GAGCCTTCGT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 125 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGAGGGTG GGACTCAAGA ACGTGTCCCT CTTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCCATGTGCG ACCCAAGTGA GGCGCTGGCT AGAGCCCTAG CGTCTTATGGTCGCCCT CGTCTTATGGTCGCCCT CGTCGTATGCTCGCCCT CGTCTTATGGTCGCCCT

[0210] SEQ ID NO: 126 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTTTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA GGCCTTGGGC AGAGCCCTAG CCAGCTANGG 120 GTACGCGTGC GAACCGTCGT ATCATGCATC ATTGGACAAG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ACGGGAAACG CCATTTCTTC 220

[0211] SEQ ID NO: 127 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAAAGGGTG GGGCTGAAAA ATGTCTCACT CTTGATCGCT GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCTGTGTGTG ATCCTAGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTACGCATGC GAGCCTTCGT ATCATGCATC TTTGGACAAG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTT 220

[0212] SEQ ID NO: 128 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA AGCTTTGGGC AGAGCCCTAG CGAGCTATGG 120 GTACGCGTGC GAGCCGTCGT ATCATGCATC ATTGGACACG GACCCCTTCT GCTCCACTTG 180 GTTAGCCGAA TGCAATGCGG ATGGGAAACG CCACTTCTTC 220

SEQ ID NO: 129 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGCGGGTG GGGCTGAAAA ATGTCTCGCT CCTCATCGCT GGTGACGACT GTCTCATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCTAGCGA GGCCCTGGCC AGAGCCCTGG GTTCGCGCTCGTATGG 120 GTACCCGCTGCATGT GAGCCCTCGCATGT GAGCCCTCGCATGT GAGCCCTCGATGT GAGCCCTCATC

SEQ ID NO: 130 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGAGAGTG GGACTGAAAA ATGTCTCGCT CCTCATCGCA GGCGACGACT GTCTGATCAT 60 ATGCGAACGG CCTGTGTGCG ACCCTAGCGA AGCCTTGGGC AGAGCCCTGG CGAGGCTCGCTCATGCGGTCGCTCGATCGATCGCTCGCTGAT

[0215] SEQ ID NO: 131 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCG GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCGAGCGA GGCCCTGGGC AGAGCCCTGG GCAGCTACGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTTT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0216] SEQ ID NO: 132 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGCGGGTG GGGCTGAAAA ACGTCTCACT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCAGTTTGTG ACCCTAGCGA AGCCTTGGGC AGAGCCTTGG CTAGGTACGG 120 GTACGCTTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GTTAGCTGAG TGTAATGCAG ACGGGAAGCG CCATTTCTTC 220

[0217] SEQ ID NO: 133 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGACTGAAAG ATGTTTCACT CCTCATAGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAATATGCG ATCCTAGTGA CGCTCTGGGC AGAGCCCTAG CTAGCTATGG 120 ATATGCGTGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAACG CCATTTCTTC 220

[0218] SEQ ID NO: 134 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGCGAGTG GGACTCAAGA ACGTTTCCCT CTTGATCGCC GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCGGTGTGCG ACCCTAGTGA GGCCTTGGGC AGAGCCCTGG CCTCGTACGG 120 GTACGCATGC GAGCCCTCAT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0219] SEQ ID NO: 135 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCCCT TCTCATAGCA GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCGATGTGTG ACCCAAGCGA GGCCCTGGGC AGAGCCCTGG CTAGCTACGG 120 GTATGCGTGC GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 136 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCG GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTCTGCG ACCCTAGCGA AGCTTTGGCT AGAGCCTTAG CTAGCTACGG 120 GTATGCCTGCATGC GAGCCTTCTGCATGC GAGCCTTCTGCATGCGAGCTTTCGATC

[0221] SEQ ID NO: 137 Length of sequence: 395 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG CGCCCTGGGG AAATAATATG CCTCGGGCAC CATGGTCACT 60 CCGGAAGGGG TACCAGTCGG TGAGAGGTAT TGTAGATCCT CAGGGGTCTT GACCACCAGC 120 GCGAGCAACT GCTTGACCTG CTACATCAAG GTGAAAGCCG CCTGTGAGAG AGTGGGGCTG 180 AAAAATGTCT CACTTCTCAT AGCGGGCGAT GACTGCTTGA TCATATGCGA ACGGCCAGTC 240 TGCGACCCTA GCGAAGCTTT GGGTAGAGCC TTAGCTAGCT ACGGGTATGC ATGCGAGCCT 300 TCGTATCATG CATCATTGGA CACGGCCCCC TTCTGCTCCA CTTGGCTTGC TGAGTGTAAT 360 GCAGACGGGA AACGCCATTT CTTCCTGACC ACGGA 395

[0222] SEQ ID NO: 138 Length of sequence: 396 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG AGCCTTGGGG AAATACTATG CTTCAGGCAC CATGGTAACC 60 CCGGAAGGGG TGCCCGTTGG TGAGAGGTAC TGTAGATCCT CGGGAGTCTT GACCACGAGC 120 GCGAGCAACT GCTTGACATG CTACATCAAG GTGAAAGCCG CTTGTGAGAG GGTGGGGCTG 180 AAAAATGTCT CCCTTCTCAT AGCAGGCGAT GACTGTTTGA TCATATGCGA ACGGCCGATG 240 TGTGACCCAA GCGAGGCCCT GGGCAGAGCC CTGGCTAGCT ACGGGTATGC GTGCGAGCCT 300 TCGTATCATG CATCACTGGA CACGGCCCCC TTCTGCTCCA CTTGGCTAGC CCGAGTGCAA 360 TGCAGATGGG AAGCGCCATT TCTTCCTGAC CACGGA 396

[0223] SEQ ID NO: 139 Length of sequence: 394 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG AGCCTTGGGG AAATAATATG CCTCGGGAAC CATGGTCACC 60 CCGGAAGGGG TGCCCGTTGG TGAGAGGTAT TGTAGATCCT CAGGAGTCTT GACGACCAGT 120 GCNAGCAACT GCTTGACCTG CTATATCAAG GTGAAAGCCG CTTGTGAGAG AGTGGGGCTG 180 AAAAATGTTT CACTCCTCAT AGCCGGCGAT GACTGTCTGA TCATATGCGA ACGGCCAGTG 240 TGCGACCCTA GCGAGGCCTT GGGCAGAGCC CTAGCCAGCT ATGGGTACGC GTGCGAACCG 300 TCGTATCATG CATCATTGGA CACGGCCCCC TTCTGCTCCA CTTGGCTTGC TGAGTGCAAT 360 GCACACGGGA AACGCCATTT CTTCCTGACC ACGG 394

[0224] SEQ ID NO: 140 SEQ Length: 394 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG CGCCCTGGGA AAATACTATG CTTCTGGCAC CATGGTCACC 60 CCGGAAGGGG TCCCCGTCGG TGAGAGGTAT TGTAGATCCT CAGGAGTGTT GACTACCAGT 120 GCAAGCAACT GCTTGACCTG TTACATTAAG GTGTCAGCAG CCTGCCAGAG AGTGGGACTG 180 AAAAATGTCT CGCTCCTCAT CGCAGGCGAC GACTGTCTGA TCATATGCGA ACGGCCTGTG 240 TGCGACCCTA GCGAAGCCTT GGGCAGAGCC CTGGCGAGCT ATGGGTATGC GTGCGAGCCT 300 TCGTATCATG CATCATTGGA CACGGCCCCC TTCTGCTCCA CTTGGCTTGC CGAGTGCAAT 360 GCACACGGGA AGCGCCATTT CTTCCTGACC NCGG 394

[0225] SEQ ID NO: 141 SEQ Length: 396 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG GGCCCTGGGG AAATACTACG CTTCTGGCAC AATGGTCACC 60 CCGGAAGGGG TGCCCGTGGG CGAGAGGTAT TGTAGATCCT CGGGAGTGCT CACTACAAGT 120 GCGAGCAACT GTCTGACCTG CTACATCAAG GTCCGAGCCG CGTGCGAGCG GGTGGGGCTG 180 AAAAATGTCT CGCTCCTCAT CGCTGGTGAC GACTGTCTCA TCATATGTGA GCGGCCTATG 240 TGCGACCCTA GCGAGGCCCT GGGCAGAGCC CTGGGTTCGT ATGGGTACGC ATGTGAGCCC 300 TCATATCATG CCTCACTGGA CACAGCCCCC TTTTGCTCCC ACTTGGCTTG CCGAGTGCAA 360 TGCAGACGGG CNGGCGCCAT TTCTTCCTGA CCACGG 396

[0226] SEQ ID NO: 142 Length of sequence: 394 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG GGCCCTCGGG AAATACTATG CCTCTGGCAC CATGGTCACC 60 CCGGAAGGGG TGCCCGTGGG CGAGAGGTAT TGTAGGTCCT CGGGGGTGTT GACCACCAGC 120 GCCAGCAACT GCTTGACCTG CTACATCAAG GTGAAAGCTG CCTGCGAGAG GGTGGGACTC 180 AAGAACGTGT CCCTTTTAAT CGCGGGCGAT GACTGTCTGA TCATATGTGA GAGGCCCATG 240 TGCGACCCAA GTGAGGCGCT GGGGAGAGCC CTAGCGTCTT ATGGGTACGC GTGTGAGCCC 300 TCATATCATG CATCATTGGA CACGGCCCCC TTCTGCTCCA CTTGGCTTGC CGAGTGCAAT 360 GCAGATGGGA AACGCCATTT CTTCCTGACC ACGG 394

[0227] SEQ ID NO: 143 Length of sequence: 394 SEQ type: nucleic acid sequence GACCATCCAG AATGGGTGCG GGCGCTTGGG AAATACTATG CCTCAGGCAC CATGGTCACC 60 CCGGAAGGAG TGCCCGTTGG TGAGAGGTAT TGTAGATCTT CAGGCGTTCT GACGACCAGC 120 GCGAGCAACT GCTTGACCTG CTACATTAAG GTGAAAGCCG CTTGCGAAAG GGTGGGGCTG 180 AAAAATGTCT CACTCTTGAT CGCTGGCGAT GACTGTCTGA TCATATGCGA ACGGCCTGTG 240 TGTGATCCTA GTGACGCCTT GGGCAGAGCC CTGGCGAGCT ATGGGTACGC ATGCGAGCCT 300 TCGTATCATG CATCTTTGGA CACGGCCCCC TTTTGCTCCA CTTGGCTTGC TGAGTGTAAT 360 GCAGATGGGA AGCGCCATTT CTTCCTGACC ACGG 394

[0228] SEQ ID NO: 144 Length of sequence: 394 SEQ type: nucleic acid sequence GACCATCCAG CCTGGGTGCG AGCCCTGGGG AAGTACTATG CTTCAGGAAC CATGGTGACC 60 CCGGAAGGGG TGCCAGTTGG TGAGAGGTAT TGTAGATCCT CGGGCGTCTT GACCACCAGT 120 GCGAGTAACT GCTTGACATG CTACATCAAG GTGAAAGCCG CCTGTGAGCG GGTGGGGCTG 180 AAAAACGTCT CACTCCTCAT AGCCGGCGAT GACTGCTTGA TCATATGTGA GCGGCCAGTT 240 TGTGACCCTA GCGAAGCCTT GGGCAGAGCC CTGGCTAGCT ACGGGTACGC TTGCGAGCCC 300 TCATATCATG CATCATTGGA CACGGCCCCC TTCTGCTCCA CTTGGTTAGC TGAGTGTAAT 360 GCAGACGGGA AGCGCCATTT CTTCCTGACC ACGG 394

SEQ ID NO: 145 Sequence length: 24 Sequence type: Nucleic acid Sequence CCGGCGCCGA MYCGGCGACC GGCC 24

SEQ ID NO: 146 Sequence length: 24 Sequence type: Nucleic acid Sequence GAGAGACATT GAAGGGCGAC GTG 23

SEQ ID NO: 147 Sequence length: 24 Sequence type: Nucleic acid Sequence CCAAAAGGTG GTGGATGGGT GATG 24

SEQ ID NO: 148 Sequence length: 24 Sequence type: Nucleic acid Sequence GGTTGGTAGG TCGTAAATCC CGGTCA 26

SEQ ID NO: 149 Sequence Length: 24 Sequence Type: Nucleic Acid Sequence TGGTAGCCAC TATAGGTGGG T 21

SEQ ID NO: 150 Sequence length: 24 Sequence type: Nucleic acid Sequence GCGACGTGGA CCGTACRTGG GCGT 24

SEQ ID NO: 151 Sequence length: 24 Sequence type: Nucleic acid Sequence GGCGACGTGG ACCGTACRTG GGCGTGATTT AGGTGACACT ATAG 44

SEQ ID NO: 152 Sequence length: 24 Sequence type: Nucleic acid sequence CTTGGTAGCC ACTATAGGTG GGTTAATACG ACTCACTATA GGG 43

SEQ ID NO: 153 Sequence length: 24 Sequence type: Nucleic acid Sequence GACATTGAAG GGCGACGTRG ACCGTAC 27

SEQ ID NO: 154 Sequence Length: 24 Sequence Type: Nucleic Acid Sequence GCCTCKGACC ATCCAGAATG GGTG 24

SEQ ID NO: 155 Sequence length: 24 Sequence type: Nucleic acid Sequence GCKAGCAACT GYTTGACCTG 20

SEQ ID NO: 156 Sequence length: 24 Sequence type: Nucleic acid Sequence CGAGYGGYCT CCGRAAGTCC GTGGTCAG 28

SEQ ID NO: 157 Sequence Length: 24 Sequence Type: Nucleic Acid Sequence RCAMACCACC AGGTCNCCRT CYTTGATGAT 30

SEQ ID NO: 158 Sequence Length: 24 Sequence Type: Nucleic Acid Sequence GTCTCCGTCY TTGATGATGG AACTGTC 27

SEQ ID NO: 159 Sequence length: 24 Sequence type: Nucleic acid Sequence CCTTTTATGG GCATGGNATN CCYCT 25

SEQ ID NO: 160 Sequence length: 24 Sequence type: Nucleic acid Sequence CCTTTTATGG GCATGGNATN CCYCTTAATA CGACTCACTA TAGGGCGA 48

SEQ ID NO: 161 Sequence length: 24 Sequence type: Nucleic acid Sequence GTCTCCGTCY TTGATGATGG AACTGTCTTA GGTGACACTA TAGAATAC 48

SEQ ID NO: 162 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGGACTGGTA GGCACCTGGT ATTCTGCCAC TCAAAGGCAG AGTGCGAGCG 60 TCTTGCCGGC CAATTCTCTT CCCGGGGAGT CAATGCCATT GCCTATTATC GGGGCAAG 118

SEQ ID NO: 163 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGGACCGGCA GGCACCTCGT ATTCTGCCAT TCTAAGGCGG AGTGCGAGCG 60 GCTTGCCGGG CAGTTTTCCT CCAGGGGAGT TCATGCTATC GCCTATTATA GGGGAAAA 118

SEQ ID NO: 164 Sequence length: 118 Sequence type: Nucleic acid sequence CGAGCGGATG CGAACCGGGA GGCACCTTGT GTTCTGCCAC TCCAAGGCAG AGTGCGAGCG 60 CCTAGCCGGC CAGTTTTCTG CTAGAGGGGT TAATGCCATC GCTTATTATA GGGGAAAA 118

SEQ ID NO: 165 Sequence length: 118 Sequence type: Nucleic acid Sequence CGAGCGGATG CGAACCGGAA GGCATNTCGT ATTTTGCCAT TCAAAGGCTG AGTGCGAACG 60 CCTTGCCGGC CAGTTTTCTG CCAGGGGGGT CAATGCTATT GCTTATTATC AGGGCAAA 118

SEQ ID NO: 166 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGAGGATG CGGACCGGCA GGCACCTCGT ATTCTGTCAC TCCAAGGCGG AGTGCGAGCG 60 CCTGGCGGGC CAGTTTTCTT CCCGGGGGGT GAATGCCATT GCCTACTACC AGGGGAAA 118

SEQ ID NO: 167 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGGATG CGGACCGGGA GGCACCTCGT ATTCTGCCAT TCAAAAGCGG AGTGCGAGCG 60 CCTTGCGGGC CAATTCTCAG CCAGGGGGGT TAATGCCATC GCCTATTATA GGGGCAAA 118

SEQ ID NO: 168 Sequence length: 118 Sequence type: Nucleic acid sequence TGAACGGATG CGGACCGGAC GGCACCTCGT GTTCTGCCAC TCTAAGGCGG AGTGCGAGCG 60 CCTAGCGGGC CAATTCTCGA GCAGGGGCGT CAATGCCATT GCCTACTATA GGGGAAAG 118

SEQ ID NO: 169 Sequence length: 118 Sequence type: Nucleic acid Sequence CGAGCGCATG CGGACTGGCC GGCACCTCGT ATTCTGCCAT TCCAAGGCGG AGTGTGAGCG 60 CTTGGCCGGC CAGTTCTCGT CCCGGGGGGT AAACGCCATT GCCTATTACA GGGGAAAG 118

SEQ ID NO: 170 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGAATG CGGACGGGCA GGCACCTCGT ATTCTGTCAT TCAAAGGCGG AGTGCGAGCG 60 TCTTGCCGGC CAGTTCTCCT CCAGGGGGGT CAATGCCATC GCCTACTATA GGGGTAAA 118

SEQ ID NO: 171 Sequence length: 118 Sequence type: Nucleic acid Sequence CGAACGGATG CGGACCGGGA GGCACCTCGT ATTCTGCCAT TCCAAGGCGG AGTGCGAGCG 60 ACTCGCGGGC CAATTCTCGG CTAGGGGGGT CAACGCCATC GCCTACTACC AGGGAAAG 118

SEQ ID NO: 172 Sequence Length: 158 Sequence Type: Nucleic Acid Sequence TGAGAGAGTG GGGCTGAAAA ATGTTTCGCT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAGTGTGCG ATCCGAGCGA CGCCTTGGGC AGAGCCCTGG CGAGCTACGG 120 GTACGCATGCATGATCATGA

[0257] SEQ ID NO: 173 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ACGTTTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA CGCCTTGGGC AGAGCCCTCG CTAGGTATGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 174 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGAGGGTG GGGCTGAAAA ATGTCTCCCT TCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTATGCG ACCCTTGCGA CGCATTGGCC AGAGCCCTCG CTAGTTATGG 120 GTATGCTCGCGATCGATCGCTCGATCGATCGATCGCTGCAT GATCTTCCTGC GATCTCTCGCTGCAT

[0259] SEQ ID NO: 175 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCTGTTTGCG ACCCTAGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTACGCTTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0260] SEQ ID NO: 176 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCGGTGTGCG ACCCGAGTGA CGCTTTGGGC AGAGCCCTAG CGAGCTATGG 120 GTACGCGTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCGG ATGGGAAACG CCATTTCTTC 220

[0261] SEQ ID NO: 177 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATCGCA GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAGTATGCG ACCCTAGTGA CGCCCTGGGC AGAGCCCTGG CGAGCTACGG 120 GTATGCGTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0262] SEQ ID NO: 178 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAATGTGCG ACCCTAGTGA CGCTTTGGGC AGAGCCCTAG CGAGCTATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCGCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCGG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 179 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTATGCG ACCCTAGTGA TGCCTTGGGT AGAGCCTTGG CGAGCTATGG GCTCGATCGATCGATCGATCGATCGTCGATCGATCGTCGATCGATCGATCGTCGATCGCATGCGTGCGACGTC

SEQ ID NO: 180 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGAGTG GGACTGAAAA ATGTTTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCCAGCGA GGCTTTAGGC AGAGCCTTGG CTAGCTACGG 120 GTACGCTCGATCGATCGATCGCTGCAT GAGCCATCCTGC GATCCATGCT

SEQ ID NO: 181 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCGGTGTGCG ATCCTAGTGA CGCCCTGGGC AGAGCCCTGG CGAGCTACGG 120 ATACGCATGCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGCATGATCGATCGCTCGATCGATCGATCGCGCATGCGATCGCTCATGCTGATGCTGTCGATCGCTCGATCGATCGCTCATGCTGATCGTCGATCTCGATCGCGCTCATGCTGATCGTCGATCTGCTGATCGATCTGTCATGCGATCGATCTGATCGTGATCGTCGATCTGTCATGCTGATCGTCGATCTGC

[0266] SEQ ID NO: 182 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCC GGAGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGTG ATCCTAGCGA AGCCTTGGGG AGAGCCCTGG CTAGCTATGG 120 ATACGCGTGC GAACCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCTGAG TGTAATGCAG ATGGGAAACG CCATTTCTTC 220

[0267] SEQ ID NO: 183 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGACTGAAGA ACGTCTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAGAGG CCTATATGTG ACCCTTGCGA TGCCTTGGGC AGAGCCCTAG CTAGCTATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ACGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 184 Sequence Length: 220 Sequence Type: Nucleic Acid Sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCT GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAATGTGCG ACCCTAGTGA CGCCCTGGGC AGAGCCCTGG CGAGCTATGCGCTCGATCGATCGATCGAGCATCGATCGATCGATCGATCGATCGATCGATCC

[0269] SEQ ID NO: 185 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGCGAGTG GGGCTGAAAA ATGTTTCGCT CCTCATAGCC GGTGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAGTGTGCG ATCCGAGCGA CGCCTTGGGC AGAGCCCTGG CGAGCTACGG 120 GTACGCGTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCGCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAACG CCATTTCTTC 220

[0270] SEQ ID NO: 186 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGCGGGTG GGACTGAAAA ACGTTTCACT CCTAATCGCA GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCAATATGCG ACCCCAGTGA GGCCTTGGGC AGAGCCCTGG CGTCATATGG 120 GTACGCGTGT GAACCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCTACTTG 180 GCTAGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 187 Sequence length: 220 Sequence type: Nucleic acid Sequence TGAGCGGGTG GGACTCAAAA ACGTTTCCCT CCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCCTAGGCAGAGCCTTGG CTTCCTGCGTAT GAGCCTGCCTGCT GAGCCTGCCTGCT GAGCCTGCCATCT GAGCCTGCT GCATCCTGCT GCATCCTGCT GCATCCTGCT GCATCCTGCT GCATCCTGCT GCATCCTGCT GCATCCTGCT GCATCCGTCC GCATCCTGCT GCTCCCTCTGATCAGCTCTCTGGCTGCGTCTGGCTGCTGCTGGCTGCTGCTGGCTGCTGCTGGCTGCTGCTGGCTGCTGCTGGCTGCTGCTGGCTGCTGCTGGCTGCTGCTGCGG

SEQ ID NO: 188 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGCGGGTG GGACTCAAGA ACGTTTCCCT CCTGATCGCG GGCGATGACT GTTTGATCAT 60 ATGTGAACGG CCCATGTGCG ATCCTAGCGA GGCCTTGGGG AGAGCCCTGG CATCGTACGG 120 GTACGCGTCTGCTTGT GAGCCTGC GCATGCGTGTGCGTGTCTGGTCGTGCTTGTGCGTGTCTGGTCGTGCGTGTGCGTGTGCGTGTGCGTGTGGTCGATCGTGCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGGTCGATCGTGCGGCTGCGGATGCGTCATGCGTCTGGTCGATCTGCTGGTCAGCTCGTTCTGGTCAGCTCGTTCTGGTCAGCTGTCTGGTCGCTGCTGGTCAGCTGTCTGGTCGTCTCTGCGTGTCTGGTCGTGCGTGTCTGGCTGCTTCT

SEQ ID NO: 189 Sequence Length: 133 Sequence Type: Nucleic Acid Sequence GAGAAGGTTA ACATTCCTCT TGTGCCTGCG GCGAGACCGC GCACGGTCCA CAGGTGTTGG 60 CCCTACCGGT GTGAATAAGG GCCCGACGTC AGGCTCGTCG TTAAACCGAG CCCATTACCC 120 GCCTGGGCAA ACG 133

SEQ ID NO: 190 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGGA GGTTAAGAAT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAATAAGGGCCCG ACGTCAGGAT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 191 Sequence Length: 137 Sequence Type: Nucleic Acid Sequence CTTAAAGGGC GGCTACGGTC CCTCTCGCGC TTATGGAGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGACCGTT 120 ATCCCCCCGGGGCACACG 137

SEQ ID NO: 192 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCC CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACT 138

SEQ ID NO: 193 Sequence length: 138 Sequence type: Nucleic acid sequence TTTAAGAGAA GGTTAAGGAT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 ATCCCRCCCG GGCAAACG

SEQ ID NO: 194 Sequence length: 138 Sequence type: Nucleic acid sequence TTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGCTAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTG GGCAAACG GGCAAACG

SEQ ID NO: 195 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTACGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTG GGCAAACG138

SEQ ID NO: 196 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGAGAA GGTTAAGATT CCTCTTACGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTG GGCAAACG 138

SEQ ID NO: 197 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 198 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGT GTGCGGCGAG AACGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGAGTGAT TCACGGCCCG ACGTCAGGAT CATCGTTAAA CCGATCCCGT 120 CACCCACCTA TACTGGCT 138

SEQ ID NO: 199 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTG TGCAAATA

SEQ ID NO: 200 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCAAA

SEQ ID NO: 201 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCAAA

SEQ ID NO: 202 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGCAAA

SEQ ID NO: 203 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGCAAA

SEQ ID NO: 204 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG AAGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCGCCTG GGCAAACG 138

SEQ ID NO: 205 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGGAT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTG GGCAAACG

SEQ ID NO: 206 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCGCCTG GGCAAACG 138

SEQ ID NO: 207 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTG GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTGAA TAAGGACCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGCAAACG

SEQ ID NO: 208 Sequence length: 220 Sequence type: Nucleic acid sequence CGATCGGGTG GGACTCAAAA ACGTGTCCCT TCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ATCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTATGG 120 GTACGCGTCGTCGTGT GAGCCTCGATCGTGTGTAGGCGTCGTCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCGTGTGCAGGTGCGTGTGCAGGTGCGTGTGCAGGTGCGTGTGCAGGTGCGGCTGCGCGTGTGCAGCTCGTCGCGTGTGCGTGCGTGTGCGGCTCGTCGCGTGTGGCTCGTCGCGTGTGGCTCGTCGCTCGTGTGCGTCGTCGCGTGTGCGTCTGCGTGTGGCTCGCTGCGTGTGCAGCTGCGTGTGGCTCGTCTCGTGTGGCTCGTCTCGTGTGGGCGCTCTCGTATGG

[0293] SEQ ID NO: 209 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAGA ACGTTTCCCT CCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CATCATACGG 120 GTATGCGTGT GAGCCCTCAT ATCATGCATC ATTGGACAAG GCCCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAACGCGG ATGGGAAGCG CCATTTCTTC 220

[0294] SEQ ID NO: 210 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCA GGAGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAGTGTGCG ACCCTAGCGA CGCTCTGGGC AGAGCCCTAG CAAGCTATGG 120 GTACGCGTGC GAGCCCTCAT ACCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0295] SEQ ID NO: 211 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTC GGACTCAAAA ACGTGTCCCT CCTAATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTACGG 120 GTATGCATGT GAGCCCTCAT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 212 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGCGGGTG GGACTCAAAA ACGTTTCCCT CCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCCTGCC AGAGCCTTGG CTTCCTGCGTAT GAGCCTGCTGCATCAT GAGCCTGCCATCGATGCGTCT GAGCCTGCCATCAT GAGCCTGCCATCATGATCGTCGCGTCAT

[0297] SEQ ID NO: 213 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAACGAGTG GGACTCAAAA ACGTTTCCCT CTTGATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGTGA GGCCTTGGGC AGAGCCCTGG CTTCATACGG 120 GTATGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACCTG 180 GTTAGCCGAG TGTAATGCAG ACGGGAAGCG CCATTTCTTT 220

[0298] SEQ ID NO: 214 SEQ Length: 220 Type of sequence: nucleic acid sequence TGATCGGGTG GGACTCAAAA ACGTGTCCCT TCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ATCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCGTATGG 120 GTACGCGTGT GAGCCCTCAT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GTTAGCCGAG TGTAATGCGG ACGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 215 Sequence length: 220 Sequence type: Nucleic acid sequence CGATCGGGTG GGACTCAAGA ACGTGTCCCT CCTGATCGCC GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCGAGCGA GGCTTTGGGC AGAGCTCTTG CCTCGTACGG GCTCGATCCGTCGATCCGTCGATCCGTCGATCTGCTGCTGCCTCCATGCGTGTGCTCGATCACTGGT

SEQ ID NO: 216 Sequence length: 220 Sequence type: Nucleic acid sequence CGATCGGGTG GGACTCAAAA ACGTTTCCCT CCTGATCGCG GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCGATGTGCG ACCCGAGCGA GGCCCTGGGC AGAGCCCTGG CATCGTACGG GCTCGATCGATCGATCGCATCTGATCGATCGATCGCTCATGCGTCATGTCCTGCGCCATGGTCAGCATGTCCTGCGCCATCATGCTGCTCTGCGCGGCT

[0301] SEQ ID NO: 217 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGTGA CGCGCTGGGC AGAGCCCTCG CGAGCTACGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GTTAGCTGAG TGTAATGCGG ATGGGAAGCG CCATTTCTTC 220

[0302] SEQ ID NO: 218 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ACGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGCG ATCCTAGTGA CGCGCTCGGC AGAGCCCTAG CTAGCTACGG 120 ATACGCGTGCGCTCGATCGCGTCGTCGCGCTCGCGCTCGCGCTCGCGCTAGCTAGCGTGCGCGCACTG

[0303] SEQ ID NO: 219 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGCGGGTG GGACTGAAAA ACGTTTCACT CCTAATCGCA GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCAATATGCG ACCCCAGTGA GGCCTTGGGC AGAGCCCTGG CGTCATATGG 120 GTACGCGTGCGCCTGATCGATCGAGATCGATCGATCGTCGATCTGATGCGTCATGCGTCATGCGTCATGCGTCATGCGTCATGCGTCATGCGTCATGCGTCATGCGTCATGCGTCATGCGG

SEQ ID NO: 220 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGAGGGTG GGACTGAAAA ACGTTTCACT CCTCATAGCA GGTGACGACT GTCTGATCAT 60 ATGTGAACGG CCGGTGTGCG ACCCTAGCGA AGCCTTGGGC AGAGCCCTGG CAAGCCGTCAGCTCATGGCT GAGCCTTCCTGCATGCATGCATGCATCGATCGATGCGG

SEQ ID NO: 221 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGACTGAAAA ATGTCTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTTTGCG ACCCTAGCGA CGCCTTGGGC AGAGCCCTGG CAAGCTATGGTCGCTCGATCGATCCGTCGATCGCTCGATCGATCGCTCGCGGTCATGCTTGCGCGCATGT

[0306] SEQ ID NO: 222 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGACTGAAGA ACGTCTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAGAGG CCTATATGTG ACCCTTGCGA TGCCTTGGGC AGAGCCCTAG CTAGCTATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ACGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 223 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCC GGAGATGACT GTCTGATCAT60 ATGCGAACGG CCAGTGTGTG ATCCTAGCGA AGCCTTGGGG AGAGCCCTGG CAAGCTATGG ATGCTGCTGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCTGCC

SEQ ID NO: 224 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCGGTGTGCG ATCCTAGTGA CGCCCTGGGC AGAGCCCTGG CGAGCTACGCTCGCTCGATCGATCGATCGATCGATCCGTCATGCTGATGCTCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGCGCGGGG

[0309] SEQ ID NO: 225 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGACTGAAAA ATGTTTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCCAGCGA GGCTTTAGGC AGAGCCTTGG CTAGCTACGG 120 GTACGCGTGC GAGCCATCGT ATCATGCATC ATTGGACACG GCCCCTTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAATGCAG ACGGGAAACG CCATTTCTTC 220

[0310] SEQ ID NO: 226 SEQ Length: 220 Type of sequence: nucleic acid sequence TCAGAGGGTG GGACTGAAAA ATGTCTCATT GCTCATAGCA GGCGACGACT GTCTGATCAT 60 ATGTGAACGG CCAGTGTGCG ACCCTTGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTATGCATGC GAGCCTTCAT ATCATGCATC ATTGGACACG GCTCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCTG ACGGGAAACG CCATTTCTTC 220

[0311] SEQ ID NO: 227 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATCGCA GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAGTATGCG ACCCTAGTGA CGCCNTGGGC AGAGCCCTGG CGAGCTACGG 120 GTATGCGTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0312] SEQ ID NO: 228 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTATGCG ACCCTAGTGA TGCCTTGGGT AGAGCCTTGG CGAGCTATGG 120 GTATGCGTGC GAACCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCTGAG TGCAATGCAG ATGGGAAACG CCACTTCTTC 220

[0313] SEQ ID NO: 229 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGGGTG GGGCTGAAAA ATGTCTCCCT TCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTATGCG ACCCTTGCGA CGCATTGGGC AGAGCCCTCG CTAGTTATGG 120 GTATGCGTGC GAACCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0314] SEQ ID NO: 230 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ACGTTTCGCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA CGCCTTGGGC AGAGCCCTCG CTAGCTATGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 231 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAATGTGCG ACCCTAGTGA CGCTTTGGTC AGAGCCCTAG CGAGCTTGTCGCTCGATCCGTCGATCGCATC GCATCTCATGCTCGATCCGTCATGCTCGCTGCTGCGGCC ATC

SEQ ID NO: 232 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCGGTGTGCG ACCCGAGTGA CGCTTTGGGC AGAGCCCTAG CGAGCTATGCTCGATCGATCCGTCGATCCGTCGATCCGTCCATGCCATCGTCCATGCCATCGCCATCGTCCATGCCATCGTCCATGCCCT

SEQ ID NO: 233 Sequence length: 220 Sequence type: Nucleic acid Sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCTGTTTGCG ACCCTAGTGA CGCCTTGGGC AGAGCCCTGG GCTCATGCTCGATCGATCGATCGTGCGTGCGCTCGGTCATGCTCGCGCTCGGTCAGGCGTGCGCTCGGTCAGGCGTGCGCTCGGTCAGGCTTGCGCGCGGTCAGGCTTGCGCGCGGTCAGGCTGGCGCTCGGCTAGTCATGCGCGCTCGGTCAGTCGTGCGCCTGGCT

[0318] SEQ ID NO: 234 SEQ Length: 220 Type of sequence: nucleic acid sequence TCAGAGGGTG GGACTGAAAA ATGTCTCATT GCTCATAGCA GGCGACGACT GTCTGATCAT 60 ATGTGAACGG CCAGTGTGCG ACCCTTGTGA CGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTATGCATGC GAGCCTTCAT ATCATGCATC ATTGGACACG GCTCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCTG ACGGGAAACG CCATTTCTTC 220

[0319] SEQ ID NO: 235 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGCGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCT GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAATGTGTG ATCCTAGCGA GGCCTTGGGC AGAGCCCTTG CCAGCTACGG 120 GTACGCATGT GAGCCTTCGT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCAG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 236 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGAGGGTG GGACTCAAGA ACGTGTCCCT CTTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCCATGTGCG ACCCAAGTGA GGCGCTGGCT AGAGCCCTAG CGTCTTATGGTCGCCCT CGTCTTATGGTCGATCGCCCT CGTCTTATGCTCGAGCCCT

[0321] SEQ ID NO: 237 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTTTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA GGCCTTGGGC AGAGCCCTAG CCAGCTANGG 120 GTACGCGTGC GAACCGTCGT ATCATGCATC ATTGGACAAG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 238 Sequence length: 220 Sequence type: Nucleic acid sequence CGAAAGGGTG GGGCTGAAAA ATGTCTCACT CTTGATCGCT GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCTGTGTGTG ATCCTAGTGA CGCCTTGGGC AGAGCCCTGG GCTCGTGCGCGTGCGCGTGCGCGTGCGCGTGCGCGTGCGTGCGCGTGCGCGTGCGTGCGCGTGCGCGTGCGCGGCT

[0323] SEQ ID NO: 239 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGGGTG GGGTTGAAAA ACGTCTCCCT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCTAGCGA AGCTTTGGGC AGAGCCCTAG CGAGCTATGG 120 GTACGCGTGC GAGCCGTCGT ATCATGCATC ATTGGACACG GACCCCTTCT GCTCCACTTG 180 GTTAGCCGAA TGCAATGCGG ATGGGAAACG CCACTTCTTC 220

SEQ ID NO: 240 Sequence length: 220 Sequence type: Nucleic acid sequence CGAGCGGGTG GGGCTGAAAA ATGTCTCGCT CCTCATCGCT GGTGACGACT GTCTCATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCTAGCGA GGCCCTGGCC AGAGCCCTGG GTTCGCGCTCGTATGG120 GTACCCGCTGCATGT GAGCCCTCGCATGT GAGCCCTCATC GCATCCCTCATGATGCGGCT

[0325] SEQ ID NO: 241 SEQ Length: 220 Type of sequence: nucleic acid sequence CCAGAGAGTG GGACTGAAAA ATGTCTCGCT CCTCATCGCA GGCGACGACT GTCTGATCAT 60 ATGCGAACGG CCTGTGTGCG ACCCTAGCGA AGCCTTGGGC AGAGCCCTGG CGAGCTATGG 120 GTATGCGTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGCAATGCAG ACGGGAAGCG CCATTTCTTC 220

[0326] SEQ ID NO: 242 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCG GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTGTGCG ACCCGAGCGA GGCCCTGGGC AGAGCCCTGG GCAGCTACGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTTT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0327] SEQ ID NO: 243 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGCGGGTG GGGCTGAAAA ACGTCTCACT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCAGTTTGTG ACCCTAGCGA AGCCTTGGGC AGAGCCTTGG CTAGGTACGG 120 GTACGCTTGC GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GTTAGCTGAG TGTAATGCAG ACGGGAAGCG CCATTTCTTC 220

[0328] SEQ ID NO: 244 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGACTGAAAG ATGTTTCACT CCTCATAGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAATATGCG ATCCTAGTGA CGCTCTGGGC AGAGCCCTAG CTAGCTATGG 120 ATATGCGTGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAACG CCATTTCTTC 220

[0329] SEQ ID NO: 245 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCCCT TCTCATAGCA GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCGATGTGTG ACCCAAGCGA GGCCCTGGGC AGAGCCCTGG CTAGCTACGG 120 GTATGCGTGC GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0330] SEQ ID NO: 246 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATAGCG GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTCTGCG ACCCTAGCGA AGCTTTGGGT AGAGCCTTAG CTAGCTACGG 120 GTATGCATGC GAGCCTTCGT ATCATGCATC ATTGGACAAG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ACGGGAAACG CCATTTCTTC 220

[0331] SEQ ID NO: 247 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGCGGGTG GGACTCAAGA ACGTTTCCCT CCTGATCGCG GGCGATGACT GTTTGATCAT 60 ATGTGAACGG CCCATGTGCG ATCCTAGCGA GGCCTTGGGG AGAGCCCTGG CATCGTACGG 120 GTACGCGTGT GAGCCTTCAT ATCATGCATC ATTGGACACG GCCCCTTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCGG ATGGAAAACG CCATTTCTTC 220

SEQ ID NO: 248 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGCGAGTG GGACTCAAGA ACGTTTCCCT CTTGATCGCA GGCGATGATT GTCTGATCAT 60 ATGTGAGCGG CCCATGTGCG ACCCTAGCGA GGCCCTAG GCAGAGTTGG CTTCGTATGG 120 GTATGCGCTCGATCGTGCGTGTGCGTCTGTGTGTGCGTCGATGCGTGTGCGTCCGTATGTGTGCGTCCGTATGCGTGCGTGTGCGTCCGTGCGTGTGCGTCCGCTCATGTGTGGCTCCGCATGTGTGCGCCCATCATGTGTGGCTCCGCATGT

[0333] SEQ ID NO: 249 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAGA ACGTTTCTCT CCTGATTGCA GGCGATGATT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCCAGCGA GGCTCTGGGA AGAGCCCTTG CCTCGTACGG 120 GTATGCATGT GAGCCCTCAT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCTGAG TGTAATGCAG ACGGGAAACG CCATTTCTTC 220

SEQ ID NO: 250 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGCGGGTG GGACTCAAGA ACGTGTCTCT CCTAATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAACGG CCCATGTGTG ACCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCCTACGG 120 ATACGCTTGCTCGATCGATCGATCGATCGTTCGTCGATCTGATGCTTGCGGATCC

[0335] SEQ ID NO: 251 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAAA ACGTGTCTCT CCTGATTGCG GGCGATGATT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCCAGCGA GGCTCTGGGG AGAGCCCTTG CCTCGTACGG 120 GTATGCATGT GAGCCCTCAT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCTGAG TGTAATGCAG ACGGGAAACG CCATTTCTTC 220

[0336] SEQ ID NO: 252 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGGGTG GGACTCAAGA ACGTTTCCCT CCTAATCGCG GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCAATGTGCG ATCCCAGTGA GGCGCTGGGC AGAGCCCTAG CGTCTTACGG 120 GTACGCGTGT GAACCCTCAT ATCATGCATC TTTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ACGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 253 Sequence length: 118 Sequence type: nucleic acid sequence GGAGAGGATG CGGACCGGCA GGCACCTCGT TTTCTGCCAC TCAAAGGCGG AGTGCGAGCG 60 TCTGGCGGGC CAGTTCTCGT CGCGGGGGGT TAATGCCATC GCCTACTATC GGGGGAAG 118

SEQ ID NO: 254 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGAATG CGAACTGGGA GGCACTTAGT GTTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 CCTAGCAGGG CAGTTTTCAG CAAGAGGGGT GAACGCCATT GCCTATTATC GGGGAAAG 118

SEQ ID NO: 255 Sequence length: 118 Sequence type: nucleic acid sequence GGAGCGGATG CGTACCGGCA GGCATCTCGT ATTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 CCTGGCGGGC CAATTCTCCT CCCGGGGGGT CAATGCCATT GCTTATTATA GGGGAAAG 118

SEQ ID NO: 256 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGAATG CGAACTGGGA GGCACTTAGT GTTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 CCTAGCAGGG CAGTTTTCAG CAAGAGGGGT GAACGCCATT GCCTATTATC GGGGAAAG 118

SEQ ID NO: 257 Sequence length: 118 Sequence type: nucleic acid Sequence GGAGCGGATG CGGACCGGCA GGCACCTCGT TTCTGGGCCC TCAAAGGCAG AATGCGAGCG60 TTTGGCGGGC CAATTCTCTT CGCGGGGGGT TAATGCCATC CCCTACTATA TAGGGAAA 118

SEQ ID NO: 258 Sequence length: 118 Sequence type: nucleic acid Sequence GGAGAGGATG CGGACCGGCA GGCATCTTGT ATTCTGCCAT TCTAAGGCGG AGTGCGAGCG 60 CCTTGCGGGT CAGTTCTCTT CACGGGGGGT CAATGCCATT GCCTATTATC GGGGAAAG 118

SEQ ID NO: 259 Sequence length: 118 Sequence type: nucleic acid Sequence GGAGAGGATG CGGACCGGTA GGCACCTCGT GTTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 CTTGGCGGGC CAGTTCTCTT CACGTGGGGT CAATGCCATC GCCTATTATC GGGGGAAG 118

SEQ ID NO: 260 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGAGGATG CGGACCGGTA GGCACCTCGT GTTCTGCCAT TCAAAGGCGG AGTGCGAGCG 60 CTTGGCGGGC CAGTTCTCTT CACGTGGGGT CAATGCCATC GCCTATTATC GGGGGAAG 118

SEQ ID NO: 261 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTG GGCAAACG

SEQ ID NO: 262 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGGA GGCTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAACGGCCCG ACGTCAGGCT CGTCGTTAAA CTGAGACCGA 120 CACCCACCTG GGCAAACG

SEQ ID NO: 263 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TCTCGGCCCG ACGTCAGGCT CGACGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCTAACT

SEQ ID NO: 264 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAATAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGACCGA 120 CACCCACCTG GGCAAACG 138

SEQ ID NO: 265 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTTAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAC 120 TAACCACCTG GGCACG

SEQ ID NO: 266 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGGATG CGGACCGGCA GGCATCTCGT ATTCTGCCAT TCTAAGGCGG AGTGCGAGCG 60 TCTGGCGGGC CAATTCTCCT CGCGGGGGGT TAATGCCATT GCCTATTATC GAGGGAAG 118

SEQ ID NO: 267 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGGATG CGGACCGGTA GGCACCTCGT ATTCTGCCAC TCAAAGGCGG AGTGTGAGCG 60 CCTGGCCGGC CAGTTCTCCT CTAGGGGCGT CAATGCCATT GCTTATTATC GGGGAAAG 118

SEQ ID NO: 268 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGGATG CGGACCGGTA GGCACCTCGT GTTCTGCCAC TCAAAGGCGG AGTGCGAGCG 60 CCTGGCGGGC CAATTCTCCT CACGAGGGGT TAATGCCATC GCCTATTATC GGGGGAAG 118

SEQ ID NO: 269 Sequence length: 118 Sequence type: Nucleic acid Sequence GGAGCGGATG CGGACCGGCA GGCATCTCGT GTTCTGCCAC TCAAAGGCGG AGTGTGAGCG60 CTTGGCGGGC CAATTTTCCT CTCGAGGGGT TAATGCCATT GCCTATTACA GGGGGAAG 118

SEQ ID NO: 270 Sequence length: 118 Sequence type: Nucleic acid Sequence TGAGCGGATG CGGACCGGGA GGCACCTCGT ATTTTGCCAT TCCAAGGCTG AGTGCGAGCG 60 TCTAGCCGGC CAGTTCTCTG CTAGAGGGGT GAACGCCATT GCCTATTATA GGGGCAAA 118

[0355] SEQ ID NO: 271 SEQ Length: 220 Type of sequence: nucleic acid sequence TGATCGGGTG GGACTCAAAA ACGTGTCCCT CCTGATCGCG GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ATCCTAGCGA GGCCCTGGGC AGAGCCCTGG CTTCGTATGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ACTGGACACA GCTCCCTTCT GCTCCACTTG 180 GCTAGCCGAG TGCAATGCTG ATGGGAAGCG TCATTTCTTC 220

[0356] SEQ ID NO: 272 SEQ Length: 220 Type of sequence: nucleic acid sequence CGATCGGGTG GGACTCAAGA ACGTTTCCCT CCTGATCGCA GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ACCCTAGCGA GGCCCTGGGC AGAGCCCTGG CTTCGTACGG 120 GTACGCGTGT GAGCCCTCAT ATCATGCTTC ACTGGACACA GCTCCCTTCT GCTCCACTTG 180 GTTAGCCGAG TGCAATGCTG ATGGGAAGCG CCATTTCTTC 220

[0357] SEQ ID NO: 273 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGCGGGTG GGACTCAAGA ACGTCTCCCT CCTGATCGCA GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCGATATGCG ATCCTAGTGA GGCCTTGGGC AGAGCCTTGG CTTCATACGG 120 GTACGCATGT GAACCCTCAT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTGGCCGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 274 Sequence length: 220 Sequence type: Nucleic acid sequence CGATCGGGTG GGGTTAAAGA ACGTATCCCT CCTGATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ACCCTAGCGA GGCTCTGGGG AAAGCCCTGG CTTCGTCATGCTCGATGCGTCGATCCGTCGCTGCTGCGCACTG

SEQ ID NO: 275 Sequence length: 220 Sequence type: Nucleic acid sequence CGATCGGGTG GGGTTAAAGA ACGTATCCCT CCTGATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCTATGTGCG ACCCTAGCGA GGCTCTGGGG AGAGCCCTGG CTTCGTATGCTCGATCGATCCGTCTCGTGCTGCTGATCCGTCTCGTGCTCGATCCGTCAGGTGCGCACTG

[0360] SEQ ID NO: 276 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCT GGCGATGACT GTCTGATCAT 60 ATGTGAACGG CCAGTGTGCG ACCCAAGCGA CGCTCTGGGC AGAGCCCTAG CAAGCTATGG 120 GTACGCGTGC GAGCCTTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 277 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGGACCGGGA GGCACTTAGT GTTCTGCCAC TCAAAGGCGG AGTGCGAGCG 60 CCTGGCGGGC CAATTTTCCT CTAGGGGGGT CAATGCCATA GCCTATTATC GGGGCAAG 118

SEQ ID NO: 278 Sequence length: 118 Sequence type: Nucleic acid sequence GGAGCGGATG CGTACCGGCA GGCACCTCGT ATTCTGTCAC TCGAAGGCAG AGTGCGAGCG 60 CTTGGCGGGC CAATTCTCCT CTAGGGGTGT CAACGCCATC GCCTATTATC GGGGGAAG 118

[0363] SEQ ID NO: 279 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGCGGGTG GGACTCAAGA ACGTTTCCCT CCTAATCGCA GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCTATATGCG ATCCGAGCGA GGCTCTGGGC AGAGCCCTCG CCTCGTACGG 120 GTACGCATGT GAGCCCTCAT ATCATGCATC ATTGGACACA GCTCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGTAATGCGG ATGGGAAGCG CCATTTCTTT 220

SEQ ID NO: 280 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGCGAGTG GGACTTAAGA ACGTTTCCCT CTTGATCGCA GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCTATGTGCG ACCCTAGTGA GGCCCTGGGC AGAGCCTTGG CTTCATATGG ATGCTCGCTCGCTCGTCGCTCGTCGCTGCTGCGTCCGCTTGGTCAGGTGCGCCTGGTCAGGTCCGCTGCTGCGGTCGCTGCCGTGGTCGCTGCTGCGGTCGTCCGCTGCTGCGGTCGTCCGCTGCTGGTCGTGGCTCGCTCCGGTCGTGGCTCGCTGGTCGCTGCTGGTCGTCCGCTGCTCTGGTCGCTGCTCGTCGCTGCTGCTGCCGCTGCTGCTGCTGGTCGCTGCTCTGGCT at GGCCTGCTGGTCGTCCGCTGCTCTGGTCGTCCGCTGCTCCGGTCCTGGCTCGTCGCTGCTCTGGTCGTCCGCTGCTCTGGTCGCTGCTCGTCGCTGCTGCTGCCTGCT Att at

SEQ ID NO: 281 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTGAA TAAGGGCCCG ACGCCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTG GGCTAACG 138

SEQ ID NO: 282 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTG GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TTCCCACCTGGGCACG

SEQ ID NO: 283 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTG GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TTCCCACCTGGGCACG

SEQ ID NO: 284 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTTAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAC 120 TAACCACCTG GGCAAACG

SEQ ID NO: 285 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGGA GGCTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAACGGCCCG ACGTCAGGCT CGTCGTTAAA CTGAGACCGA 120 CACCCACCTG GGCAAACG 138

SEQ ID NO: 286 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGACCGA 120 CACCCACCTG GGCAAACG 138

SEQ ID NO: 287 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TCTCGGCCCG ACGTCAGGCT CGACGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCTAACT

SEQ ID NO: 288 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG

SEQ ID NO: 289 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGTT CGTCGTTAAA CCGAGCCCGC 120 TACCCACCTG GGCAAACG

SEQ ID NO: 290 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence TTTAAGAGAA GGTTAAGATT CCTTTTGTGC CTGCGGCGAG ACCGGGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCGGGGCAAA

SEQ ID NO: 291 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CAGCGGCGGA AACGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGA 120 CACCCACCTGCGCACGCT

SEQ ID NO: 292 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG

SEQ ID NO: 293 Sequence length: 138 Sequence type: Nucleic acid Sequence TTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTGGGCAAA

SEQ ID NO: 294 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTTTTGTGC CTGCGGCGAG ACCGCGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACGTG GGCACACG GGCACACG138

SEQ ID NO: 295 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGGA AACGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACGTG GGCAAACG

SEQ ID NO: 296 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGCT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCCGGGCAAA

SEQ ID NO: 297 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG

SEQ ID NO: 298 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGC 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 299 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCGCCTG GGCAAACG

SEQ ID NO: 300 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAC 120 CAACCTCCCG GGCAAACG

SEQ ID NO: 301 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAAAGTT GGTTAAGGTT CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAC 120 TACCCTCCTGCGCACGCT

SEQ ID NO: 302 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 303 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCATCTG GGCAAA

SEQ ID NO: 304 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGGTAA TAAGGCCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 305 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAAAGTT GGTTAAGGTT CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAC 120 TACCCTCCTG GGCAAA

SEQ ID NO: 306 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCTAACT 138

SEQ ID NO: 307 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTGTTGTGC CTGCGGCGAG ACCGCGCACG GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACGGTCGTG GTCAAACG 138

SEQ ID NO: 308 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 309 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTGAA TAAGGACCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGC 120 TACCCACCTG GGCAAACG

SEQ ID NO: 310 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGGT GGTTAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGAT CGTCGTTAAA CCGAGCCCAT 120 TACCCTCCTG GGCAAACG

SEQ ID NO: 311 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCt CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGAA

SEQ ID NO: 312 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGA CGTCAGGCTC GTCGTTAAAC CGAGCCCGCA 120 ACCCACCTGGCACG

SEQ ID NO: 313 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC ATGCGGCGAG AACGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 CACCCACCTGGGCAAACG

SEQ ID NO: 314 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAATAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 AACCCGCCTG GGCAAACG

SEQ ID NO: 315 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTATGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAATAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGC 120 ACCCACCTGG GCAAACG CGCAAACG137

SEQ ID NO: 316 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGAA GGTCAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTTAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGACCGC 120 CACCCACCTG GGCAAACG 138

SEQ ID NO: 317 Sequence length: 138 Sequence type: Nucleic acid sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAA AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGCACG

SEQ ID NO: 318 Sequence Length: 138 Sequence Type: Nucleic Acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGCTAA TAAGGGCCCG ACGTCAGGTT CGTCGTTAAA CTGAGCCCGT 120 CACCCACCTG GGCAAACG

SEQ ID NO: 319 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACA GTCCGCAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 320 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG GTCCACAGGT 60 GCTGGCCTTA CCGGTGTGAA TAAAGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT 120 TACCCACCTG GGCAAACG 138

SEQ ID NO: 321 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAGGGTT GGTCAAGGTC CCTCTGGCGC TTGTGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA CCGAGCCCAT 120 TACCCACCTGGGCAAA

[0406] SEQ ID NO: 322 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGTCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCGGTGTGCG ATCCGAGCGA AGCTTTAGGC AGAGCCTTGG CTAGCTATGG 120 GTACGCGTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0407] SEQ ID NO: 323 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGGGTG GGACTGAAAA ATGTCTCTCT CCTCATAGCA GGCGATGACT GTTTGATCAT 60 ATGCGAACGG CCAGTTTGCG ACCCTAGCGA GGCCTTGGGA AGAGCCTTGG CTAGCTATGG 120 GTACGCTTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTAGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0408] SEQ ID NO: 324 Sequence length: 221 Sequence type: Nucleic acid sequence TGAGAGGGTG GGACTGAAAG ATGTTTCACT CCTCATAGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGCGG CCAATATGCG ATCCTAGTGA CGCTCTGGGC AGAGCCCTC CTAGCTATTGCTGCCTCATGCTCATGCCATCGTCTGCTCGTCATCTGCTGCTCATC

[0409] SEQ ID NO: 325 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGTGATGACT GTCTGATCAT 60 ATGTGAACGG CCAATATGCG ACCCCAGCGA AGCCTTAGGA AGAGCTTTGG CTAGCTACGG 120 GTACGCGTGTGAGCCTGATCGATCGAG ATCGATCGATCGATCGATCGATCATCGATCTGCATGCGATCGATCTGCATGCATGATCTAGCATGATCGATCGATCGCATGCATCCTGCATGCGATCCGATCGTCGATCTGCGTCGATGCGTCGATCTGCGTCGATGCGTCGATCTGCTGCGATCTGGATGCGATCGTCGATCTGCGTCGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGATCTGCGTCGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGATCTGGATGCGTCGCTGCAT at Ascent

SEQ ID NO: 326 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGAGGGTG GGGCTGAAAA AtGTCTCACT CCTCATAGCC GGCGATGACT GCTTGATCAT 60 ATGCGAACGG CCAGTTTGCG ATCCTAGTGA CGCTTTGGGC AGAGCCCTAG CGAGCTTGCATGCGCTCGCGTCGTCATGCGTCGTCTCGCGGTC ATCGCTTGCGCGCTCCGTCATGCGCGCTCCGTCATGCGCGCTCCGTCATGCGCGCTCCGTCATGCGCTCTCGCGGTC ATC

[0411] SEQ ID NO: 327 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ATGTCTCTCT CCTCATAGCC GGCGATGACT GTTTGATCAT 60 ATGTGAACGG CCAGTGTGCG ACCCTAGTGA AGCCTTGGGC AGAGCCCTAG CTAGCTATGG 120 GTACGCGTGC GAGCCTTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

[0412] SEQ ID NO: 328 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGCGAGTG GGGCTGAAAA ATGTCTCACT CCTCATCGCA GGCGACGACT GTTTGATCAT 60 ATGCGAACGG CCTGTGTGCG ACCCTAGTGA AGCCTTGGGC AGAGCCCTGG CGAGCTCATCGTCGTCGCGTGCGTGCGTGCGATCGCGTGCGTGCGATCGCGTGCGTGCGATCGCGTGCGATCGTGCGATCGCGTGCGATCGCATGC 120

[0413] SEQ ID NO: 329 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATAGCC GGCGATGACT GTCTGATCAT 60 ATGCGAACGG CCTGTATGCG ATCCTAGTGA AGCCCTGGGC AGAGCCCTGG CGAGCTATGG 120 ATATGCGTGC GAGCCTTCGT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTTGCCGAG TGTAATGCGG ATGGGAAGCG CCATTTCTTC 220

[0414] SEQ ID NO: 330 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGCGGGTG GGACTGAAAA ACGTTTCACT CCTAATCGCA GGCGATGACT GCTTGATCAT 60 ATGTGAGCGG CCAATATGCG ACCCCAGTGA GGCCTTGGGC AGAGCCCTGG CGTCATATGG 120 GTACGCGTGT GAACCCTCAT ATCATGCATC ATTGGACACG GCCCCCTTCT GCTCTACTTG 180 GCTAGCCGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 331 Sequence length: 138 Sequence type: Nucleic acid sequence CTTCAAGGGG AGGTAATGGT CCCTCTAGAG CTTATGGAAG GAAAGCGCAC GGTCCACAGG 60 TGTTGGCCCT ACCGGTGTAA TAAGGGCCCG GCGCTAGGCA CGCCGTTAAA CCGAGACCGC 120 CATCCCCCTGGGCACG

SEQ ID NO: 332 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAGGCTA GGCAATGGTC CCTCTTGCGC TTATGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTAAT AAGGACCCGG CGCTAGGCAC GCCGTTAAAC CGAGCCCGTT 120 ATCCCCCTGG GCAACG

SEQ ID NO: 333 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAGGGGA GGTAAAGGTC CCTCTAGAGC GTATGGAGGG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTAAT AAGGACCCGG CNCTAGGCAC GCCGTTAAAC CGAGACCATT 120 ATCCCCCTGG GCAAACG 137

SEQ ID NO: 334 Sequence length: 137 Sequence type: Nucleic acid sequence CTTAAAGGGA GGCTACGGTC CCTCTTGCGC TTATGGCGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGCCCGTT 120 ATCCCCCTGG GCAAACG

SEQ ID NO: 335 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAAGGGA GGCTACGGTC CCTCTCGCGC TTACGGAGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGACCGTT 120 ATCCCCCTGGCT

SEQ ID NO: 336 Sequence length: 137 Sequence type: Nucleic acid sequence CTTAAGGGGA GGTAATGGTC CCTCTCGCGC TTATGGAAGG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTAAT AAGGACCCGG CGCTAGGCAC GCCGTTAAAC CGAGTCCGTT 120 ATCCCCCTGG GCACG

SEQ ID NO: 337 Sequence length: 138 Sequence type: Nucleic acid Sequence CTTAAAGGGA GGATACGGTC CCTCTCGCGC TTATGGAGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGACCGTT 120 ATCCCCCCCGGGCACG

SEQ ID NO: 338 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAAGGGC GGCTACGGTC CCTCTCGCGC TTACGGAGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGACCGTT 120 ATCCCCCTGG GCAAACG 137

SEQ ID NO: 339 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAAGGGC GGATACGGTC CCTCTCGCGC TTACGGAGAG AAAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGACCGTT 120 ATCCCCCTGGCTCACGCG 137

SEQ ID NO: 340 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAAGGGA GGATACGGTC CCTCTAGAGC TTATGGCGAG ACAGCGCACG GTCCACAGGT 60 GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC CGAGCCCGTT 120 ATCCCCCTGG GCAAACG

SEQ ID NO: 341 Sequence length: 137 Sequence type: Nucleic acid Sequence CTTAAGGAGA GGCAACGGTC CCTCTCGCGC TTATGGAGGG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGTGTAAT AAGGACCCGG CGCTAGGCAC GCCGTTAAAC CGAGCCCGTT 120 ATCTCCCTGG GCAAGA

SEQ ID NO: 342 Sequence length: 137 Sequence type: Nucleic acid Sequence af107 TTTAAAGGGA GGCAACGGTC CCTCTCGCGC ATATGGAGGG AAAGCGCACG GTCCACAGGT 60 GTTGGTCCTA CCGGAGTAAT AAGGACCCGG CGCTAGGCAC GCCGTTAAAC CGAGCCCGTC 120 ATCCCCCCGGTC

[0427] SEQ ID NO: 343 SEQ Length: 220 Type of sequence: nucleic acid sequence CCAGAGGGTG GGGCTGAAAA ATGTCTCACT CCTCATCGCA GGCGATGACT GCTTGATCAT 60 ATGCGAAAGG CCAATATGCG ACCCTAGTGA GGCCTTGGGC AGAGCCCTGG CTTCATATGG 120 GTACGCGTGT GAGCCTTCGT ATCATGCATC ACTGGACACG GCCCCCTTCT GCTCCACTTG 180 GCTCGCCGAG TGCAATGCAG ATGGGAAGCG CTGTTTCTTT 220

SEQ ID NO: 344 Sequence length: 220 Sequence type: Nucleic acid sequence CGAACGGGTG GGACTGAAAA ATGTTTCACT CTTGATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCAATATGCG ACCCTAGTGA GGCATTGGGC CGAGCCCTGGTCGATCCGTCCTGGTCGATCGCTCGTCCTGGTCGATCCTCGTCATGTGCCCACT

SEQ ID NO: 345 Sequence length: 220 Sequence type: Nucleic acid Sequence CCAGAGAGTG GGGCTGAAAA ACGTCTCACT TCTCATCGCG GGCGATGACT GTCTGATCAT 60 ATGCGAGAGG CCAATATGCG ACCCGAGCGA AGCCCTGGGC CGAGCCCTGG GTTCCTATGG 120 GTACGCGTCT GTCGCGTGT GCGCGTCGTCGTGTGCGCGTGCTTGTGAGCCCTCATGT

SEQ ID NO: 346 Sequence length: 220 Sequence type: Nucleic acid sequence TGAGCGAGTG GGTCTGAAAA ATGTCTCGCT CCTCATTGCT GGCGACGACT GTCTCATCAT 60 ATGTGAGCGG CCCATATGCG ACCCGAGCGA GGCCTTGGCT AGAGCCCTGG CTTCGTAGGG 120 GTACGCGTCTGCGTGTGTGCGTCGCTGCGTGTGCGTCTGCGTGTGCGTCTGCGTGTGGCTGCGCATGT

SEQ ID NO: 347 Sequence length: 220 Sequence type: Nucleic acid Sequence CGAGCGAGTG GGACTGAAAA ATGTCTCACT CTTGATCGCG GGCGATGACT GCTTGATCAT 60 ATGTGAGAGG CCGATATGCG ACCCTAGCGA AGCCCTGGGC CGAGCCCTCG GTTCGTACGG GCTCGATCGATCCGTCGATCCGTCGATCCGTCTCGCTCGATCCGTCTCGTGCGCTCACGCTGATGCGTCCGCTCACGCTGATGCGCCCACGTCCGCGTCCTCGTGCTCGCGTCGTCTCGCGTGCGTCCGCTCACGTCGGTCCGCTCCATGCGTGTGCGCCTGCGCGATCTCGCGTGCGCTCGCGTCCGTGTGCGCCCACT

[0432] SEQ ID NO: 348 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAGAGGGTG GGACTGAAAA ATGTTTCACT CCTTATCGCG GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCCATTTGCG ACCCGAGCGA GGCCTTGGGC AGAGCCCTGG CGTCGTACGG 120 GTATGCTTGT GAGCCCTCAT ATCATGCATC ACTGGACACT GGCCCCTTCT GCTCCACTTG 180 GCTCGCTGAG TGCAATGCAG ATGGGAAGCG CCATTTCTTC 220

SEQ ID NO: 349 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGAGGGTG GGGCTGAAAA ACGTCTCACT CCTCATCGCG GGCGATGACT GCTTGATCAT 60 ATGCGAGAGG CCAATGTGTG ACCCTAGCGA GGCCTTGGGC AGAGCCCTGG CTTCGTAGGG 120 GTACG CGC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GCC GTC GTC GTC GCC GTC GTC GCC GTC GTC GTC GTC GTC GTC GCC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GTC GCC TCC GTC GTC GCC GTC GTC GCC TCC GTC GTC GCC TCC GTC GTC GCC GTC GCC TCC GTC GTC GCC GTC GCC TCG GTC GCC TCG TCG TCG TCG TCG TCG TCG TCG TCG TCG TGC TCG TCG TGT

SEQ ID NO: 350 Sequence length: 220 Sequence type: Nucleic acid sequence CGAACGGGTG GGACTGAAAA ATGTTTCACT CTTGATCGCT GGCGATGACT GTTTGATCAT 60 ATGTGAGCGG CCCATATGCG ACCCCAGCGA AGCCTTGGCT AGAGCCCTCG GTTCGCTCGCGTCATGATCGCTCGTCATCAT GATCCCTCGATCATGATCGATCGATCGATGCGATCGTCATGATCGATGCGATCTGCTAGTCGATCGTCATGATCC

[0435] SEQ ID NO: 351 SEQ Length: 220 Type of sequence: nucleic acid sequence TGAGAGAGTG GGGCTGAAAA ACGTCTCGCT CCTCATCGCA GGCGATGACT GTCTCATCAT 60 ATGTGAGCGG CCTATATGCG ACCCGAGCGA GGCCCTGGGG AGAGCCCTGG CCTCGTAGGG 120 GTACGCGTGT GAGCCCTCAT ATCATGCATC ACTGGACACA GCCCCCTTCT GCTCCACTTG 180 GCTTGCTGAG TGCAATGCAG ACGGGCGGCG CCATTTCTTC 220

SEQ ID NO: 352 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGAGAGTG GGGCTGAAAA ATGTCTCACT TCTCATCGCG GGCGATGACT GTCTGATCAT 60 ATGCGAGAGG CCGATATGTG ACCCGAGCGA GGCCTTGGGG AGAGCCTTGG CTTCGTACGG 120 GTACGCGTGCTTGT GAGCTCTCGT GTCGCGTGCTTGT GAGCCCTC ATGTGCGTGTGCGTGTGCGCGTGTGTGCGCGTCTGGTCGCGTGTGCGCGTCTGGTCGCGTGTGCGCGTGTGAGCTCGCGGTCAGGTGTGCGTGTGCGCGTGCGTGTGAGCTCGCTTGTGAGCTCGCTTGTGAGCTCGCTTGTGAGCTCGCTTGTGGTCGCGTGTGCGCGTGTGGTCGCTGCTGGTCGCGTGCTTGTGGTCGCTGCTGGTCGCGTGCTTGTGGTCGCTCGTCTGGTCGCTGCTGGTCGCGTGCTTGT

[0437] SEQ ID NO: 353 SEQ Length: 220 Type of sequence: nucleic acid sequence CGAACGGGTG GGGCTGAAAA ATGTCTCGCT CTTGATCGCG GGTGATGACT GGTTGATCAT 60 ATGTGAGCGG CCAATATGCG ACCCTAGCGA GGCCCTGGGC CGAGCCCTTG GTTCGTACGG 120 GTACGCGTGT GAGCCCTCAT ATCATGCATC ATTGGACACG GCTCCCTTCT GCTCCACTTG 180 GTTAGCTGAG TGTAATGCAG ATGGGAAACG CCATTTCTTC 220

[0438] SEQ ID NO: 354 Sequence length: 220 Sequence type: Nucleic acid sequence CGAACGGGTG GGACTGAAAA ATGTTTCACT CTTGATCGCT GGCGATGACT GTCTGATCAT 60 ATGTGAGAGG CCAATATGCG ACCCTAGTGA CGCATTGGGC CGAGCCCTGGCTCGCT CGTCCTCATGCGCCT CGTCCTCATGCGCCT CGTCCTGTGCGCCT CGTCCTCATGCGCACT CGTCCTGTGCCCACT

SEQ ID NO: 355 Sequence length: 220 Sequence type: Nucleic acid sequence CCAGAGGGTG GGACTGAAAA ACGTGTCACT CCTCATAGCG GGCGATGACT GTTTGATCAT 60 ATGCGAGAGG CCTATATGCG ATCCGAGCGA GGCCCTGGTC CGAGCCCTGGTCTCGATCGCTCGCGTCGTTGATCGCGCTCGTGTGTGTGCGCTCGTGCGTGTGCGCTCGTGCGTGTGCGCTCGTGCGTGTGAGCTCATGT

SEQ ID NO: 356 Sequence length: 25 Sequence type: nucleic acid Sequence CTCTTTGTGG TAGTAGCCGA GAGAT 25

SEQ ID NO: 357 Sequence length: 24 Sequence type: nucleic acid Sequence CGAATGAGTC AGAGGACGGG GTAT 24

SEQ ID NO: 358 Sequence Length: 24 Sequence Type: Nucleic Acid Sequence CAAATGATGC TGAGGACGGG GTGT 24

SEQ ID NO: 359 Sequence length: 28 Sequence type: nucleic acid Sequence TCTTCTCATC TGAGCTGCTC TCAGTAAC 28

SEQ ID NO: 360 Sequence length: 29 Sequence type: Nucleic acid Sequence AGGTGGGTAA TGGGCTCGGT TTAACGACG 29

SEQ ID NO: 361 Sequence length: 24 Sequence type: nucleic acid Sequence GACCTTGACC AACCCTTAAG ACCC 24

SEQ ID NO: 362 Sequence length: 22 Sequence type: nucleic acid Sequence TTTAACGGCG TGCCTAGCGC CG 22

[Brief description of the drawings]

FIG. 1. Non-AB- performed using the primer of the present invention.
It is a figure which shows the quantitative result of CDEF hepatitis virus.

FIG. 2 is a diagram showing HCV RNA levels and non-ABCDCEF hepatitis virus levels before and after interferon-α-treatment in hepatitis patients.

FIG. 3 shows genotypes of hepatitis patients in each region and HGV / GBV-C RNA in serum classified by the method of Example 7 of the present invention.
FIG. 6 is a diagram showing a relationship between the number of copies and the number of copies.

FIG. 4 is a view showing a band pattern for each classification system when a non-ABCDCEF virus is digested with a restriction enzyme BsmF1 and then subjected to electrophoresis.

FIG. 5 is a view showing a pattern of bands for each classification system when a non-ABCDCEF hepatitis virus is digested with a restriction enzyme ScrF1, and then subjected to electrophoresis.

FIG. 6 is a diagram showing a band pattern for each classification system when a non-ABCDCEF hepatitis virus is digested with a restriction enzyme BsmA1 and then subjected to electrophoresis.

FIG. 7 is a systematic diagram of non-ABCDCEF hepatitis virus classified based on the 5 ′ untranslated region according to the method of the present invention.

FIG. 8 is a systematic diagram of non-ABCDCEF hepatitis virus classified based on the NS3 region according to the method of the present invention.

FIG. 9 is a systematic diagram of non-ABCDCEF hepatitis virus classified based on the NS5b region by the method of the present invention.

FIG. 10 is a phylogenetic diagram of non-ABCDCEF hepatitis viruses classified based on the NS5B region by the method of the present invention.

 ────────────────────────────────────────────────── ─── Continued on the front page (72) Inventor Masafumi Mizogami 3-7-7 Nagata, Chiryu-shi, Aichi

Claims (17)

[Claims] 1. Sequence numbers 1 to 144 and 1 in the sequence listing
A nucleic acid fragment comprising 10 or more consecutive nucleotides of the nucleotide sequence shown in any one of 62 to 355. 2. Sequence numbers 1 to 144 and 1 in the sequence listing
2. The nucleic acid fragment according to claim 1, comprising 15 or more consecutive nucleotides of the nucleotide sequence shown in any of 62 to 355. 3. The nucleic acid fragment according to claim 2, which has a nucleotide sequence represented by any one of SEQ ID NOs: 145 to 161 and 356 to 358 in the sequence listing. 4. A primer for detecting a non-ABCDCEF hepatitis virus, comprising the nucleic acid fragment according to claim 1 or 2. 5. The primer according to claim 4, wherein the number of nucleotides is 15 to 50. 6. The primer according to claim 5, which has a nucleotide sequence represented by any one of SEQ ID NOs: 145 to 161 and 356 to 358 in the sequence listing. 7. A non-ABCDE comprising performing PCR using at least two primers according to any one of claims 4 to 6 and detecting an amplification product.
-A method for detecting hepatitis F. 8. The method according to claim 7, wherein the method is carried out using a primer having a base sequence represented by SEQ ID NO: 157 in the sequence listing and a primer having a base sequence represented by SEQ ID NO: 159 in the sequence listing. 9. The method according to claim 7, wherein the method is carried out using a primer having a base sequence represented by SEQ ID NO: 158 in the sequence listing and a primer having a base sequence represented by SEQ ID NO: 159 in the sequence listing. 10. A primer having the nucleotide sequence of SEQ ID NO: 150 in the Sequence Listing and SEQ ID NO: 148 in the Sequence Listing.
The method according to claim 7, wherein the method is carried out using a primer having a base sequence represented by: 11. A primer having the nucleotide sequence of SEQ ID NO: 150 in the sequence listing and SEQ ID NO: 149 in the sequence listing.
The method according to claim 7, wherein the method is carried out using a primer having a base sequence represented by: 12. A primer having the nucleotide sequence of SEQ ID NO: 154 in the sequence listing and SEQ ID NO: 156 in the sequence listing.
The method according to claim 7, wherein the method is carried out using a primer having a base sequence represented by: 13. A primer having the nucleotide sequence of SEQ ID NO: 155 in the sequence listing and SEQ ID NO: 156 in the sequence listing.
The method according to claim 7, wherein the method is carried out using a primer having a base sequence represented by: 13. A primer having a base sequence represented by SEQ ID NO: 356 in the Sequence Listing, a primer having a base sequence represented by SEQ ID NO: 357 in the Sequence Listing, and a primer having a base sequence represented by SEQ ID NO: 358 in the Sequence Listing. The method of claim 7, wherein the method is performed using 14. A non-ABCCD- labeled nucleic acid having a base sequence represented by SEQ ID NO: 359 in the sequence listing.
Probe for detecting the NS5A region of hepatitis EF virus. 15. SEQ ID NOs: 360 to 362 in the sequence listing
A probe for detecting the 5 'untranslated region of non-ABCDCEF hepatitis virus, which is obtained by labeling a nucleic acid having a nucleotide sequence represented by any of the above. 16. The 5 ′ untranslated region of the non-ABCDCEF hepatitis virus is digested with restriction enzymes having cleavage sites for BsmF1, BsmA1 and ScrFI, and based on the cleavage pattern. Classifying the non-ABCDEF hepatitis virus into four types,
Classification method of hepatitis EF virus. 17. The method according to claim 16, wherein the method is classified into four types of 1, 2, 3a, and 3b according to the following criteria. 1 is cut with ScrFI, yielding fragments of about 108, 40 and 34 bp, detecting fragments of about 108 or 40 bp.
2 is uncleaved or cleaved with ScrFI, about 149,3
Generate a 4 bp fragment and detect a 184 or 149 bp fragment. And 157, 26b cut with BsmFI
The fragment of p is detected. 3a is not cleaved with ScrFI or is cleaved to about 149,3
Generate a 4 bp fragment and detect a 184 or 149 bp fragment. And not cut by BsmFI and Bsm
Being cut with AI to produce fragments of about 75, 55, 53 bp,
A 75 or 53 bp fragment is detected. 3b is not cleaved with ScrFI or is cleaved to about 149,3
Generate a 4 bp fragment and detect a 184 or 149 bp fragment. Not cut with BsmFI, not cut with BsmAI, or a cut fragment of about 128, 55 bp is detected.