JPH1062436A - Method and apparatus for dispensing liquid - Google Patents

Abstract

PROBLEM TO BE SOLVED: To transfer a liquid to a specific dispenser case using an existing pipeter regardless of the size of a case containing the liquid. SOLUTION: When a liquid in one container is dispensed to another container using a pipeter 2 arranged with a plurality of removable dispensation chips 3, 3,... in a row at a constant pitch, the liquid is sampled from a liquid container 8 having diameter larger than the pitch of the dispensation chips 3 using the pipeter 2. The sampled liquid is then transferred to a dispensation container 9 having diameter equal to the pitch of the dispensation chips 3.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for dispensing liquid in a container to a dispensing container using a pipettor having a plurality of dispensing tips arranged in a row at equal pitch intervals. The present invention relates to a liquid dispensing method and apparatus capable of transferring a liquid from a container having a diameter larger than the pitch interval to another dispensing container.

[0002]

2. Description of the Related Art For example, in testing human immunodeficiency virus, hepatitis B or C, a test tube having a predetermined diameter and length containing a liquid such as blood obtained from a subject is used. A plurality of the liquid containers are inserted and held in holding holes formed at regular intervals in the vertical and horizontal directions of the rack member, and the liquids in the plurality of liquid containers aligned in the horizontal direction of the rack member are respectively held by the pipettor. After aspirating by the dispensing tip, the operation of discharging the sucked liquid to all the wells arranged in a row of the dispensing container consisting of the microplate at a time is repeated, and thereafter, the liquid is diluted in each well. , A test reagent is dropped, and the mixture is stirred and mixed, and the presence or absence of the antibody or antigen is qualitatively or quantitatively measured.

[0003] The dispensing apparatus used for such immunological measurement holds a plurality of dispensing tips composed of nozzle members for sucking and discharging the liquid in the liquid container in a horizontal line as described above. Pipetter, a rack member formed with holding holes for holding a large number of liquid containers at regular intervals in the vertical and horizontal directions, receives the liquid sucked by the pipettor, and contains a specific antigen or antibody in the liquid A dispensing container for performing a reaction as to whether or not the dispensing tip has been inserted, and a tip rack for holding dispensing tips to be replaced each time liquid is sucked and discharged in an aligned state.

[0004] As shown in FIG. 5, a pipettor 22 of this dispensing apparatus 21 has twelve dispensing tips 2.
, Are arranged so as to be freely replaceable in a horizontal row at a pitch of 9 mm. Therefore, the holding holes 25 for holding the liquid container in the rack member 24,
25, well 27 forming dispensing container 26,
The pipettes 22 are arranged in a row at a pitch of 9 mm, which is the same as the pitch between the dispensing tips 23 of the pipettor 22,
In the vertical direction, a plurality of rows are basically formed at an equal pitch of 9 mm. The number of pipetting tips of a pipettor is generally 8 to 12.

Further, since the liquid container used in this dispensing apparatus is generally a test tube having a diameter of 9 mmφ, the inner diameter of the holding hole formed in the rack member substantially matches the outer diameter of the liquid container. The intervals between the holding holes arranged in a horizontal line corresponding to the pitch of 9 mm, which is the interval between the dispensing tips, are also provided so that the distance between the centers of the holding holes is 9 mm. As described above, this liquid container generally has a diameter of 9 mm, but a large liquid container having a diameter of 13.2 mmφ such as a vacuum blood collection tube may be used.

[0006]

In the above dispensing apparatus, a pipettor for dispensing a liquid from a specific liquid container to another dispensing container is extremely expensive. Therefore, the conventional dispensing apparatus also determines the size (diameter) of the holding hole of the rack member and the well forming the dispensing container based on the pitch of 9 mm, which is the horizontal spacing of the dispensing tips provided detachably on the pipettor. Have been. As a result, the horizontal space between the holding holes formed in the rack member is also 9 between the central portions.
mm and the diameter of a general-purpose liquid container is also about 9 mm, so the distance between adjacent holding holes is very narrow, and symbols etc. for identifying the subject are described on the outer peripheral surface of each liquid container. It is necessary to carefully perform the operation of inserting and holding the liquid container in the rack member because the identification label is attached to the rack member, and it takes time.If each liquid container is not properly held in the rack member, There is a problem that the liquid to be inspected cannot be reliably sucked by the dispensing tip, which may hinder the measurement.

On the other hand, when the liquid to be inspected is contained in a large-diameter liquid container such as the above-mentioned 13.2 mmφ having a diameter exceeding 9 mmφ, the liquid container may be held by a general rack member. Since it is not possible, the liquid contained at the time of inspection is temporarily transferred to a 9 mmφ liquid container that can be used by a pipettor constituting the dispensing apparatus. However, this transfer operation is extremely complicated and takes time for inspection.

On the other hand, it is conceivable to use a rack member provided with a holding hole of a large pitch capable of holding the large liquid container. Cannot be used. For this reason, it is necessary to use either an expensive variable-pitch pipetter in which the pitch interval of the dispensing tips is variable, or dispensing one by one using a pipettor having a single dispensing tip. In any case, they have to be greatly constrained in terms of economy, time and efficiency.

In view of such circumstances, the present invention makes it possible to simply and easily transfer a liquid to a specific dispensing container using an existing pipettor regardless of the size of the container containing the liquid. It is an object of the present invention to provide a liquid dispensing method that can be used and a dispensing device used for the dispensing method.

[0010]

According to one aspect of the present invention, there is provided a pipettor in which a plurality of dispensing tips are detachably arranged in a horizontal line at equal pitch intervals. When dispensing the liquid in the liquid container to another dispensing container using the above, the liquid is collected from the liquid container having a diameter larger than the pitch interval between the dispensing tips using the pipetter, and collected. A liquid dispensing method characterized in that the liquid is transferred to a dispensing container having the same diameter as the pitch interval between dispensing tips.

According to a fifth aspect of the present invention, the liquid in the liquid container is transferred to another dispensing container by using a pipettor in which a plurality of dispensing tips are removably arranged in a horizontal line at equal pitch intervals. In dispensing, a liquid container having a diameter larger than the pitch interval between the dispensing tips, a front row corresponding to the dispensing tips at odd-numbered positions of the pipettor, and a rear row corresponding to the dispensing tips at even-numbered positions. And placed as a pair,
These pairs of front and rear liquid containers are alternately arranged in a plurality of rows in the vertical direction.First, liquid is collected from the liquid container in the front line using the dispensing tip at the odd-numbered position, and the collected liquid is collected. A plurality of wells having the same diameter as the pitch interval between pipetting tips of the pipettor are arranged in a horizontal row at the same pitch interval as the pitch interval, and the same dispensing container is formed by arranging a plurality of rows in the vertical direction. Dispense into every other well in the row, then take the liquid from the liquid vessel in the back row by the dispensing tip at the even-numbered position, and then discharge into every other empty well in the same row, all of the wells in the same row A liquid dispensing method characterized by transferring a liquid to a liquid.

According to a sixth aspect of the present invention, there is provided a pipettor having a plurality of dispensing tips arranged in a row at a predetermined pitch interval, and a liquid dispenser having a pitch interval equal to the pitch interval of the dispensing tips of the pipettor. The holding holes for holding the liquid containers containing the liquid containers and the small holes for empty suction by the dispensing tip are alternately provided in a horizontal row, and the front row is held at a predetermined interval from the front row formed. A rack member provided with a pair of rear rows provided so that the positions of the holes and the small holes are symmetric to each other, and a plurality of rows provided in the vertical direction alternately with the pair of front rows and rear rows,
Using a dispensing apparatus consisting of a pipe corresponding to the number of dispensing tips of the pipettor and a dispensing container having a plurality of dispensing containers provided in a plurality of rows in a vertical direction, first, a liquid in a front row held by a rack member The liquid in the container is aspirated by the corresponding dispensing tip, and at the same time, the dispensing tips corresponding to the small holes in the same row are sucked empty, and then the liquid in the front row sucked by the dispensing tip is placed in one of the dispensing containers. Discharge into the wells
Next, the liquid in the liquid container in the rear row is sucked by the corresponding dispensing tip, respectively, and at the same time, the dispensing tips corresponding to the small holes in the same row are sucked empty, and the liquid in the rear row sucked by the dispensing tip is discharged into the dispensing container. A liquid dispensing method characterized by discharging into every other empty well in the same row and dispensing the liquid to each well in the same row by a two-step operation.

One of the liquid dispensing apparatuses according to the present invention comprises a pipettor having a plurality of dispensing tips detachably arranged in a horizontal line at a predetermined pitch interval, and a pipetter having a pitch interval between the dispensing tips. The liquid container having a larger diameter is arranged in a front row corresponding to the dispensing tip at the odd-numbered position of the pipetter, and a rear row corresponding to the dispensing tip at the even-numbered position to form a pair. And a rack member capable of alternately arranging a plurality of rows of liquid containers in the vertical direction, and wells having the same diameter as the pitch interval between the pipetting tips of the pipettor are arranged in a horizontal row at the same pitch interval as the pitch interval. A liquid dispensing device comprising a plurality of dispensing containers.

Further, one of the liquid dispensing apparatuses according to the present invention comprises a pipettor having a plurality of dispensing tips arranged in a horizontal line at a predetermined pitch interval; At the same interval, a predetermined row is provided for a front row in which holding holes for holding a liquid container having a diameter larger than the pitch interval and small holes for empty suction by the dispensing tip are alternately arranged in a horizontal row. In order to hold a liquid container in which a plurality of rows of the front row and the rear row are alternately arranged in the vertical direction, the rear row provided as a pair so that the positions of the holding holes and the small holes in the front row are symmetrical to each other at intervals. A liquid member comprising: a rack member; and a dispensing container in which a plurality of wells having the same diameter as the pitch interval between the dispensing tips of the pipettor are arranged in a horizontal row at the same pitch interval as the pitch interval. Dispensing device A.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION Liquid dispensing according to the present invention
The dispensing is performed using a pipettor in which a plurality of dispensing tips are detachably arranged in a horizontal line at equal pitch intervals, and a liquid container for storing liquid has a diameter larger than the pitch interval between the dispensing tips. Then, the liquid collected from the liquid container using the pipettor is placed in a well of a dispensing container in which a large number of wells having substantially the same diameter as the pitch interval between the dispensing tips are arranged in a number of vertical and horizontal directions. It is a thing to transfer.

A pipettor for dispensing a liquid contained in a liquid container to each well of a dispensing container is composed of eight to twelve dispensing tips each composed of a nozzle member and arranged in a horizontal line at equal pitch intervals (generally, The existing pipettor provided at 9mm pitch) is used for immunoassay, etc. The pipetter that has once sucked and discharged liquid such as blood is to be replaced with a new one. A tip rack for holding a dispensing tip is provided, but the tip rack is not necessarily required for a liquid that does not require replacement of the dispensing tip.

The liquid container containing the liquid to be transferred has a diameter larger than the pitch interval between the dispensing tips arranged in a row in a pipettor to be used. It is at least less than twice the pitch interval between dispensing tips. A pipettor can be used even if the diameter of the liquid container exceeds twice the pitch between the dispensing tips, but the adjacent dispensing tips must be inserted into the liquid container when collecting liquid with the dispensing tips. Is not preferred.

As described above, assuming that the pitch interval between the pipetting tips of the pipettor used for liquid dispensing is 9 mm, basically, the liquid containers will be arranged in the same number as the dispensing tips in a horizontal line. Then, a liquid container having a diameter of 9 mm or more cannot be used. In the present invention, since a liquid container having a diameter of 9 mm or more, which is the pitch interval between the dispensing tips of the existing pipettor, is used by using the existing pipetter, the liquid pipes are arranged in a row at equal pitch intervals as before. The use of a liquid container having a diameter larger than the pitch interval between the dispensing tips is enabled by using every other dispensing tips without simultaneously using the dispensing tips arranged in the above. .

More specifically, assuming that the pipetting tips used have a uniform pitch of 9 mm between pipetting tips.
The diameter of the holding hole for holding the liquid container of the rack member is set to one, and for example, the diameter of the holding hole (hereinafter referred to as “small hole”) corresponding to the even number of the dispensing tip is 9 mm or less, or dispensing. The diameter of the holding hole corresponding to the odd number of the dispensing tip of the pipettor is set to 9 mm or more by setting the diameter so that the tip of the tip can be inserted or by eliminating the tip.
The use of a liquid container having a large diameter is enabled by setting the diameter to less than mm.

Now, the pitch between the dispensing tips of the pipettor is p, the radius of the inner diameter of the holding hole formed in the rack member is R, the radius of the small hole is r, and the outer peripheral edge of the holding hole and the outer peripheral edge of the small hole are R. Assuming that a gap formed therebetween is c, p = R + r + c = 9 mm
(However, r and c may be 0.). According to this relational expression, as shown in FIGS. 3 and 4, when r of the small hole 7 for accepting and sucking the tip end of the dispensing tip corresponding to the even number is set to be small in the minimum necessary limit, the odd number The R of the holding hole 6 corresponding to the dispensing tip can be set larger than the conventional 4.5 mm. Therefore, if the small hole r is 1 mm, the holding hole R can be 8 mm, so that the outer diameter of the sample container is 16 m.
mφ can also be used. Therefore, even with a 13.2 mmφ vacuum blood collection tube that once needs to be transferred to a normally used 9 mmφ sample container, a 9 mm pipettor between dispensing tips can be sufficiently used.

The liquid container is held by a rack member when used. The rack member has a holding hole having an inner diameter substantially equal to the outer diameter of the liquid container to be held in the front row at a position corresponding to the odd number of the dispensing tip, and the rear row at a position corresponding to the even number of the dispensing tip, respectively. Provided at equal intervals,
The front row and the rear row are arranged as a pair and a plurality of rows are arranged in the vertical direction. At the position corresponding to the even-numbered dispensing tip between the holding holes in the front row, a small hole into which the tip of the even-numbered dispensing tip can be inserted, and the odd-numbered dispensing tip between the holding holes in the rear row. Small holes into which the tips of the odd-numbered dispensing tips can be inserted are provided at equal pitch intervals at positions corresponding to the dispensing tips. Therefore, the holding holes and the small holes are arranged at the same pitch in both the front row and the rear row, and the holding holes and the small holes in the front row and the rear row are arranged symmetrically with each other.

The dispensing container for dispensing the liquid in the liquid container has a large number of wells having the same diameter as the pitch interval between the dispensing tips of the pipettor arranged in the horizontal and vertical directions. If the pitch of the dispensing tips is 9 mm and 8 stations, 8 wells are provided in a horizontal row at a pitch of 9 mm. If 12 pipes, 12 wells are provided in a horizontal row. A known microplate can be used.

In order to dispense the liquid contained in the liquid container into the dispensing container, first, all the liquid containers are inserted and held in the rack member, and then the pipetter is positioned above the front row, and then automatically or manually. When the dispensing tip is lowered, the tip of the dispensing tip on the liquid container is inserted into the liquid container, but the tip of the dispensing tip located on the small hole is guided into the small hole. When the pipetter is operated in this state, the dispensing tip whose tip is inserted into the liquid container sucks up the liquid, but the dispensing tip corresponding to the small hole is in an empty sucking state.

When the liquid in the liquid container is sucked up by every other dispensing tip, the dispensing tip is lifted upward, and the pipetter is moved onto the dispensing container and stopped on the required well. Thereafter, the dispensing tip is lowered to discharge the liquid into every other well. At this time, the liquid is not discharged into the well corresponding to the dispensed tip sucked empty.

Next, after the pipetting operation is performed to move the dispensing tip to the rear row, the above operation is repeated, and the liquid in the liquid container is sucked up by the previously dispensed tip and the liquid is discharged. The dispensing tip is sucked empty. Thereafter, operating the pipettor to move the dispensing tip onto the well from which the liquid was ejected in the previous operation, and ejecting the liquid from the dispensing tip, all the liquid in the liquid container is dispensed into each well in the same row. Noted. After that, by performing the same operation, the liquid having the front row and the rear row as one unit is changed to 2 units.
By the step operation, it is possible to dispense into the wells in the same row of the dispensing container. In this dispensing operation, it is preferable to automatically operate the pipetter according to a predetermined program, and discharge the specimen into all the wells of the dispensing container.

[0026]

According to the liquid dispensing method of the present invention, a pipette in which a plurality of dispensing tips are arranged in a row at equal pitch intervals has a diameter at most approximately twice the pitch interval between the dispensing tips. The liquid can be collected and dispensed from the liquid container. In addition, the dispensing device of the present invention replaces a rack member that constitutes the dispensing device according to the size of the liquid container that stores the liquid, thereby temporarily distributing the liquid to another pipetting device that constitutes the dispensing device. It can be dispensed without transferring to the sample container.

[0027]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a description will be given of a case where the liquid dispensing method and apparatus of the present invention is applied to an immunoassay method for testing blood obtained from a subject. As shown in FIG. 1, the dispensing apparatus 1 according to the embodiment includes a rack member 5 for holding a liquid container 8 containing blood in an aligned state, and a liquid dispensing device disposed in front of the rack member 5. The dispensing container 9 is composed of a microplate, and the rack member 5 and the pipettor 2 that moves horizontally above the dispensing container 9 and moves up and down on the rack member 5 and the dispensing container 9.

The pipettor 2 constituting the dispensing apparatus 1 is composed of a nozzle member whose diameter is reduced from the base end to the tip end.
1 to 12 (12 in FIG. 1) dispensing tips 3
It is detachably held in a horizontal line at equal pitch intervals of mm, and performs vertical movement and horizontal movement under the control of a predetermined program.

As shown in FIGS. 1 and 2, this pipettor 2 is replaced with a new dispensing tip when the blood in the liquid container 8 is sucked up by the dispensing tip 3 and dispensed into the dispensing vessel 9. A tip rack 4 capable of accommodating a large number of dispensing tips in a row and in a vertical direction is provided.

The liquid container 8 containing the blood to be dispensed is held by the rack member 5. This rack member 5
When forming the holding holes 6 for holding the liquid containers 8 corresponding to the dispensing tips 3, 3,..., Every other holding hole 6 having a radius R of 6 mm as shown in FIGS. Six small holes with a radius r of 1 mm are provided in a horizontal line at a pitch of 18 mm and have a size such that the tip of the dispensing tip 3 can be inserted into the center between the holding holes 6 and 6. 7 are provided as a front row X, and a predetermined interval (9 mm pitch in the embodiment) is present in the front row X and in the vertical direction (in the drawing, all behind), and the holding holes 6 and The holding holes 6 and the small holes 7 are alternately provided in a horizontal row so that the positions of the small holes 7 are symmetrical to form a rear row Y. The front row X and the rear row Y are paired, and the front row X and the rear row Y are alternately arranged in the vertical direction. 7 pairs (7 rows each for the front row and the rear row) Which was formed by presence Ji), holding holes 6 for holding the liquid container 8 is provided in total 42 pieces.
Incidentally, 42 small holes 7 are similarly formed.

Therefore, the pitch interval between the holding holes 6 and the small holes 7 is 9 mm in each of the front row X and the rear row Y. The pitch interval due to the alternate arrangement of the horizontal holding holes 6 and the small holes 7 is the same as that of the pipettor. 2 corresponds to the dispensing tips 3 arranged in a horizontal line at a pitch of 9 mm. As a result, it is possible to hold the liquid container 8 without looseness, and in consideration of the detachability from the holding hole, it is possible to hold 42 liquid containers 8 having a diameter of about 10 to 11.5 mmφ on the rack member 5 for measurement. .

On the other hand, a dispensing container 9 for dispensing blood from the liquid container 8 has wells 10, 10,... Each having a hemispherical concave portion on the upper surface capable of receiving the blood in the liquid container 8 by the dispensing tip 3. Are provided in a horizontal row at a pitch of 9 mm in correspondence with the twelve dispensing tips 3, and a total of 84 vertical rows are provided.

The measurement using the dispensing apparatus 1 having such a configuration is performed in the following procedure. 1) Liquid containers 8, 8,..., Each of which is a test tube containing blood obtained from a subject, are inserted and set in holding holes 6, 6,. 2) The first row of dispensing tips 3, 3, 3,... Held in the tip rack 4 is attached to the pipettor 2. 3) After moving the pipettor 2 on the front row X of the first row at the left end of the rack member 5 in the drawing, the liquid container 8 is moved down so that the tip of each dispensing tip 3 can suck up blood. Insert inside. At that time, a total of six liquid containers 8 are held every other one from the first, so FIG.
Only the dispensing tips 3 corresponding to the sample numbers 1, 3, 5, 7, 9, and 11 are inserted into the liquid container 8, and the other dispensing tips 3 are placed between the holding holes 6, 6. It merely corresponds to the small holes 7 formed. 4) In this state, when the pipetter 2 is actuated and sucked up, only the blood of the sample numbers 1, 3, 5, 7, 9 and 11 of FIG. 2 contained in the liquid container 8 held in the holding section 6 Are sucked up by the dispensing tips 3, 3, 3.
At this time, each dispensing tip 3 corresponding to the small hole 7 in the first row 3,
3 ... sucks empty. 5) When the aspirating of the sample liquid in the liquid container 8 of the first front row X by the dispensing tip is completed, the well 1 of the first row of the dispensing container 9 is moved by raising and horizontal movement of the pipettor 2.
The sample liquid is ejected into every other well 10 by moving to the positions 0, 10, 10,.... 6) Next, the pipetter 2 is returned to the rack member 5 side, and is set on the second row (back row Y) of the rack member 5, and the liquid container 8 held by the holding section 6 in the same manner as in the operation 3) above. The blood of the sample numbers 2, 4, 6, 8, 10, and 12 is aspirated by the corresponding dispensing tips 3, 3, 3,. At this time, the dispensing tips 3, 3,... Corresponding to the small holes 7 in the rear row Y perform an empty suction operation in the same manner as described above. 7) When the suction of the sample liquid in the liquid container 8 in the rear row Y is completed,
The pipette 2 is moved upward by the horizontal movement and moved to the top of the wells 10, 10, 10,... In the first column of the dispensing container 9, and the sample liquid is placed in every other empty well 10 left by the previous operation. Is discharged. This completes the dispensing of the blood in the liquid containers 8 held in the front row Y and the rear row Y into all the wells 10, 10,... Of the first row of the pipetting container 9. 8) When the operation of the above 7) is completed, the pipettor 2 is returned to the tip rack 4 and all the dispensing tips 3, 3... Are removed, and then the pipette 2 prepared in the first row of the tip rack 4 is removed. Note Load each chip. 9) The operations (2) to (8) are performed for the third row (front row) and the fourth row (back row) of the rack member 5, and the entire wells 10 of the second row of the dispensing container 9 are filled. After the sample is ejected, the same operation is performed for the liquid containers in the fifth and sixth rows.

Although the present invention has been described in connection with the case where the blood obtained from the subject is applied to an immunological measurement method to be tested, the liquid to be dispensed is other than blood. Of course, there is no particular limitation on the use.

[0035]

According to the liquid dispensing method of the present invention, a pipetter having a plurality of dispensing tips arranged in a row at equal pitch intervals from a container having a diameter larger than the pitch interval between dispensing tips is used. The liquid can be easily and easily transferred to another dispensing container.

In particular, a holding hole for holding a liquid container containing a liquid to be measured at the same pitch interval as the dispensing tip corresponding to the dispensing tip arranged at a predetermined pitch interval on the pipettor. And, small holes for empty suction are alternately provided in a horizontal row to form a front row, and the holding holes and the small holes are alternately provided in a horizontal row so as to be symmetrical with the front row at a predetermined interval in the vertical direction. By using a pair of rear members and a rack member having a plurality of pairs of alternating front and rear members, a rack member suitable for holding a liquid container containing a liquid to be dispensed can be selected very easily. By this means, the liquid can be efficiently dispensed from the liquid container to the dispensing container with one pipettor regardless of the size of the liquid container without separately using an expensive pipettor.

The dispensing apparatus of the present invention has a holding hole for holding a sample container corresponding to a pitch interval of a dispensing tip provided on a pipetter constituting the dispensing apparatus, and a dispensing tip for performing an empty suction. Small holes are alternately provided in a horizontal row to form a front row, a predetermined distance from the front row, and a rear row provided in a horizontal row so that the positions of the holding holes and the small holes in the front row are symmetrical. Since a rack member is used in which the front and rear rows are alternately arranged in the vertical direction, a single pipettor accommodates liquid containers of different sizes without changing the pitch interval between dispensing tips. The dispensed liquid can be dispensed into a dispensing container.

[Brief description of the drawings]

FIG. 1 is a perspective view of a dispensing device used in a dispensing method of the present invention.

FIG. 2 is an explanatory view showing a dispensing method by the dispensing device in FIG. 1;

FIG. 3 is an explanatory view showing a relationship between a holding hole and a small hole of the rack member of the present invention.

FIG. 4 is an explanatory view showing a relationship between a holding portion and a small hole of another rack member of the present invention.

FIG. 5 is an explanatory view showing a relationship between a pipetter, a rack member, and a dispensing container in a conventional dispensing apparatus.

[Explanation of symbols]

 Reference Signs List 1 dispensing device 2 pipettor 3 dispensing tip 4 tip rack 5 rack member 6 holding hole 7 small hole 8 liquid container 9 dispensing container 10 well

Claims (8)

[Claims] When dispensing a liquid in a liquid container to another dispensing container using a pipettor in which a plurality of dispensing tips are removably arranged in a horizontal line at equal pitch intervals, the dispensing tip is used. Using the pipetter to collect liquid from a liquid container having a diameter larger than the pitch interval between the pipettes, and transferring the collected liquid to a dispensing container having the same diameter as the pitch interval between dispensing tips. Liquid dispensing method to be used. 2. The liquid according to claim 1, wherein the liquid container has a diameter larger than a pitch interval between dispensing tips of the pipettor and less than twice as large as the pitch interval. Dispensing method. 3. The liquid container has a pair of a front row corresponding to a pipetting tip at an odd-numbered position of the pipetter and a rear row corresponding to a pipetting tip at an even-numbered position, and the front row and the rear row of the pair are alternately arranged. 3. The liquid dispensing method according to claim 1, wherein a plurality of rows are arranged in a vertical direction. 4. The dispensing container, a plurality of wells having the same diameter as the pitch interval between the dispensing tips of the pipettor are arranged in a horizontal row at the same pitch interval as the pitch interval,
2. The liquid dispensing method according to claim 1, wherein a plurality of rows are arranged in a vertical direction. 5. A dispensing tip for dispensing a liquid in a liquid container to another dispensing container using a pipettor in which a plurality of dispensing tips are detachably arranged in a horizontal line at equal pitch intervals. Liquid containers having a diameter larger than the pitch interval between them, the front row corresponding to the dispensing tips at odd-numbered positions of the pipettor, and the rear row corresponding to the dispensing tips at even-numbered positions, as a pair, A plurality of liquid containers consisting of a pair of front and rear rows are alternately arranged in a plurality of rows in the vertical direction.First, liquid is collected from the liquid containers in the front row using the dispensing tips at the odd-numbered positions, and the collected liquid is pipetted. A plurality of wells having the same diameter as the pitch interval between the dispensing tips are arranged in a horizontal row at the same pitch interval as the pitch interval,
And this is discharged to every other well of the same row of the dispensing container formed by arranging a plurality of rows in the vertical direction, and then collecting the liquid from the liquid container in the rear row by the dispensing tip at the even numbered position, A liquid dispensing method characterized by discharging to every other empty well in the same row and transferring the liquid to all the wells in the same row. 6. A pipettor in which a plurality of dispensing tips are provided in a horizontal row at a predetermined pitch interval, and a liquid container containing liquid at the same pitch interval as the pitch interval of the dispensing tips of the pipettor. The holding holes in the front row and the positions of the small holes are spaced apart from each other by a predetermined distance with respect to the front row formed by alternately providing the holding holes and small holes for empty suction by the dispensing tip in a row. The rear row provided so as to be symmetrical is a pair, the pair of front rows and the rear row are alternately provided in a plurality of rows in the vertical direction, and the well corresponding to the number of dispensing tips of the pipettor is one horizontal row. Using a dispensing device consisting of a dispensing container provided in a plurality of rows in the vertical direction,
The liquid in the liquid container in the front row held by the rack member is suctioned by the corresponding dispensing tip, and at the same time, the dispensing tips corresponding to the small holes in the same row are sucked empty, and then the front row suctioned by the dispensing tip is performed. The liquid is discharged into every other well of the dispensing container, and then the liquid in the liquid container in the rear row is sucked by the corresponding dispensing tip, and the dispensing tips corresponding to the small holes in the same row are sucked empty. Then, the liquid in the rear row sucked by the dispensing tip is discharged into every other empty well in the same row of the dispensing container, and the liquid is dispensed to each well in the same row by a two-step operation. Liquid dispensing method. 7. A pipettor in which a plurality of dispensing tips are detachably arranged in a horizontal line at a predetermined pitch interval, and a liquid container having a diameter larger than the pitch interval between the dispensing tips, The dispensing tips at odd-numbered positions are arranged in a front row corresponding to the dispensing tips at even-numbered positions, and the rear row is arranged in pairs corresponding to the dispensing tips at even-numbered positions. A rack member that can be arranged in a row, and a dispensing container in which a plurality of wells having the same diameter as the pitch interval between the dispensing tips of the pipettor are arranged in a horizontal row at the same pitch interval as the pitch interval. A liquid dispensing device characterized by the above-mentioned. 8. A pipettor in which a plurality of dispensing tips are arranged in a horizontal line at a predetermined pitch interval, and a pipe having a diameter larger than the pitch interval at the same interval as the pitch interval of the dispensing tips of the pipettor. With respect to the front row in which holding holes for holding a liquid container and small holes for empty suction by the dispensing tip are alternately provided in a horizontal row, the holding holes and the small holes in the front row are spaced apart from each other by a predetermined distance. A pair of rear rows provided so that the positions are symmetrical to each other, a rack member for holding a liquid container in which the pair of front rows and rear rows are alternately arranged in a plurality of rows in a vertical direction, and a pipetting tip of the pipetter. A liquid dispensing device comprising: a dispensing container in which a plurality of wells having the same diameter as the pitch interval are arranged in a row in a row at the same pitch interval as the pitch interval.