JPH1052405A - Method for examining hair growth - Google Patents
Method for examining hair growthInfo
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- JPH1052405A JPH1052405A JP22779696A JP22779696A JPH1052405A JP H1052405 A JPH1052405 A JP H1052405A JP 22779696 A JP22779696 A JP 22779696A JP 22779696 A JP22779696 A JP 22779696A JP H1052405 A JPH1052405 A JP H1052405A
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- Prior art keywords
- hair growth
- hair
- mouse
- site
- period
- Prior art date
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、育毛物の毛周期に
おける成長期を延長する効果を特定するための育毛検定
方法に関する技術分野に属する。より詳細には、その体
表に特徴的な発毛をするヌードマウスを用いて、その発
毛部位の状態を特定することで育毛物の毛周期における
成長期を延長する効果を検定する育毛検定方法に関する
技術分野に属する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention belongs to the technical field of a hair growth assay method for determining the effect of extending the anagen phase in the hair cycle of a hair growth product. More specifically, a hair growth assay that tests the effect of prolonging the anagen phase in the hair cycle of a hair growth product by identifying the state of the hair growth site using nude mice with hair growth characteristic of the body surface. Belongs to the technical field of methods.
【０００２】[0002]
【従来の技術】毛髪の一本一本は、成長期，退行期及び
休止期を経る、いわゆる「毛周期」と呼ばれるサイクル
に従って成長、脱落を繰り返すものであり、正常の毛周
期を営んでいる頭皮では成長期毛と休止期毛の割合が一
定以上に保たれている。これに対して、例えば男性型脱
毛症の場合は、様々な原因で成長期が短くなったり、毛
包が縮小したりする結果、相対的に休止期毛の割合が多
くなって、毛が細くなったり、長く伸びることができな
くなったりする結果、見た目の毛髪量が減少し、脱毛等
を生じるものである。高齢化社会、ストレス社会といわ
れる現代社会では、頭部毛髪が上記脱毛等の危機にさら
される機会がますます多くなっている。2. Description of the Related Art Each hair repeatedly grows and falls according to a so-called "hair cycle" which undergoes a growth period, a regression period, and a rest period, and performs a normal hair cycle. In the scalp, the ratio of anagen hair to telogen hair is maintained at a certain level. In contrast, for example, in the case of androgenetic alopecia, the growth period is shortened due to various causes, or as a result of the hair follicles shrinking, the proportion of telogen hair becomes relatively large and the hair becomes thin. As a result, the apparent amount of hair decreases and hair loss or the like occurs. In a modern society called an aging society and a stressed society, there are more and more opportunities for the head hair to be exposed to the aforementioned danger of hair loss and the like.
【０００３】それに対応して、現在までに種々の養毛剤
が種々の観点から提供されており、今後もその傾向は続
くことは明らかである。これらの養毛剤の多くは、毛周
期における成長期を延長させたり、成長期への誘導を促
進させたりするものである。そして、当然開発されつつ
ある養毛剤は、ヒトへの投与に先立ち動物に対する育毛
効果等を検討するのが常であり、現在マウスやウサギの
体毛に覆われている部分を毛刈りして、その部分に養毛
剤を塗布して、発毛、すなわち成長期の誘導効果を検討
することが多く行われている。しかしながら、この方法
は成長期の誘導効果を検討することに対しては適してい
るが、成長期の延長効果を検討することは煩雑であり、
また困難である。また、ヌードマウスの免疫寛容性を利
用して植毛試験も行われているが、この方法は、非常に
手間がかかる上に、植毛した毛自体の性質（感受性）の
ばらつきにより、大量の薬剤の評価法としては不適当な
点は否めない。[0003] In response, various hair restorers have been provided from various viewpoints, and it is clear that the trend will continue in the future. Many of these hair restorers extend the anagen phase in the hair cycle or promote induction into the anagen phase. Hair growth agents that are naturally being developed are usually examined for their effect on hair growth on animals prior to administration to humans. It is often practiced to apply a hair tonic to the hair to examine the hair growth, ie, the effect of inducing the growth phase. However, although this method is suitable for examining the effect of inducing the anagen, it is troublesome to examine the effect of prolonging the anagen.
It is also difficult. Hair transplantation tests have also been performed using the immune tolerance of nude mice. However, this method is very time-consuming and, because of the variation in the properties (sensitivity) of the hair itself, large amounts of drugs can be used. There is no denying that it is inappropriate as an evaluation method.
【０００４】[0004]
【発明が解決しようとする課題】そこで、本発明が解決
すべき課題は、養毛剤等の養毛物の簡便かつ確実な毛周
期における成長期の延長効果のインビボの検定手段を提
供することにある。SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a means for in vivo assay of the effect of extending the anagen phase in the hair cycle of a hair growth product such as a hair growth agent in a simple and reliable manner. .
【０００５】[0005]
【課題を解決するための手段】本発明者は、この課題の
解決に向けて鋭意検討を行った。その結果、その背部に
特徴的な発毛をするヌードマウスを用いて、その発毛部
位の状態を特定することで養毛剤等の養毛物の毛周期に
おける成長期を延長する効果を検定する育毛検定方法を
提供し得ることを見出した。Means for Solving the Problems The present inventor has made intensive studies to solve this problem. As a result, using a nude mouse that has a characteristic hair growth on its back, hair growth is tested by identifying the state of the hair growth site to test the effect of extending the growth period in the hair cycle of hair growth products such as hair growth agents. It has been found that an assay method can be provided.
【０００６】すなわち、本発明者は本願において、ヌー
ドマウスに育毛物を投与し、このヌードマウスの体表の
発毛部位の状態を特定して、育毛物の毛周期における成
長期を延長する効果を検定する育毛検定方法を提供す
る。[0006] That is, the present inventors, in the present application, administer a hair growth product to a nude mouse, specify the state of the hair growth site on the body surface of the nude mouse, and extend the growth period in the hair cycle of the hair growth product. And a hair growth test method for testing hair growth.
【０００７】[0007]
【発明の実施の形態】以下、本発明の実施の形態につい
て説明する。本発明は、ヌードマウスを用いた育毛検定
方法に関する。ヌードマウスとは、N.R.Grist によって
アルビノ系のマウス集落で発見された無毛のマウスのこ
とをいい、一般的にはこの無毛と共に胸腺の先天的欠損
が報告されているマウスのことをいう。本発明において
は、胸腺の先天的欠損の有無に関わらず、先天的に外観
上無毛のマウスのことをヌードマウスという。Embodiments of the present invention will be described below. The present invention relates to a hair growth assay method using a nude mouse. A nude mouse refers to a hairless mouse discovered in an albino mouse colony by NRGrist, and generally refers to a mouse that has been reported to have this hairlessness and congenital thymus deficiency. In the present invention, a mouse that is innately hairless in appearance is referred to as a nude mouse regardless of the presence or absence of a congenital thymus defect.
【０００８】本発明に適用可能なヌードマウスは、原則
的には無毛であるが、その体表に経時的にその発毛部位
が移動する特徴的な発毛をするヌードマウスである。例
えば、ヌードマウスｂａｌｂ ｃ ｎｕ／ｎｕは、ある
期間（毛周期の成長期に伴って）、ごく狭い範囲で帯状
の発毛が見られ、発毛した毛は２〜３日程度で順次抜け
落ち、その結果、発毛部位は頭から尾の方へ向かって経
時的に移動して行く。このヌードマウスにおいて、発毛
部位の広さと発毛部位の移動速度を特定，あるいは特定
部位での発毛期間を測定することによって、毛周期の成
長期の長さを検定することができる。[0008] The nude mouse applicable to the present invention is a hairless hair mouse which is hairless in principle, but has a characteristic hair growth in which the hair growth site moves over time on the body surface. For example, in the nude mouse balb nu / nu, a band-like hair growth is observed in a very narrow range for a certain period (along with the growth period of the hair cycle), and the hair that has grown hair falls sequentially in about 2 to 3 days. As a result, the hair growth site moves over time from the head to the tail. In this nude mouse, the length of the anagen phase of the hair cycle can be assayed by specifying the width of the hair growth site and the moving speed of the hair growth site, or measuring the hair growth period at the specific site.
【０００９】本発明の育毛検定方法を適用することが可
能な育毛物は、特に限定されず、育毛効果が認められた
物質を直接用いることも可能であり、市販用又は病院用
に処方された育毛剤を用いることも可能である。そし
て、本発明の育毛検定方法は、毛周期の延長効果を検定
する必要性に応じて本発明育毛検定方法を用いることが
できる。また、企図する育毛物の投与方法に応じて、本
発明育毛検定方法におけるヌードマウスへの投与方法を
選択することができる。すなわち、上記ヌードマウスに
対しては、外皮に対する塗布，散布等の投与方法を採る
ことは勿論、経口投与，皮内投与，筋注，静注等の投与
方法も採ることができる。The hair growth product to which the hair growth assay method of the present invention can be applied is not particularly limited, and a substance having a hair growth effect can be directly used, and is formulated for commercial use or for hospital use. It is also possible to use a hair restorer. The hair growth assay method of the present invention can use the hair growth assay method of the present invention according to the necessity of assaying the effect of extending the hair cycle. Further, the administration method to the nude mouse in the hair growth assay method of the present invention can be selected according to the intended administration method of the hair growth product. That is, to the nude mouse, not only the administration method such as application to the outer skin and spraying but also the administration method such as oral administration, intradermal administration, intramuscular injection and intravenous injection can be employed.
【００１０】以下、上記ヌードマウスｂａｌｂ ｃ ｎ
ｕ／ｎｕを用いた本発明育毛検定方法について例示す
る。このヌードマウスを用いた本発明育毛検定方法は、
例えば発毛部位の面積及び状態並びに発毛維持日数を具
体的な指標にすることで、育毛物の毛周期における成長
期を延長する効果を検定することを目的とした、ヌード
マウスの体表の発毛部位の状態の特定を行うことができ
る。Hereinafter, the nude mouse balb c n
The hair growth assay method of the present invention using u / nu will be exemplified. The hair growth assay method of the present invention using this nude mouse,
For example, by using the area and state of the hair growth site and the number of days of hair growth as specific indices, the purpose of testing the effect of extending the anagen phase in the hair cycle of the hair growth product is to examine the body surface of the nude mouse. The state of the hair growth site can be specified.
【００１１】（１）発毛部位の面積及び状態の把握につ
いて：試験期間中の発毛部位の面積を把握することによ
り、育毛物の発毛効果若しくは成長期延長効果を把握す
ることができる。すなわち、試験期間を通じての発毛部
位が大きければ、育毛物の発毛効果若しくは成長期延長
効果が大きいことを示し、逆に小さければこれらの効果
が小さいことを示す。(1) Ascertaining the area and state of the hair growth site: By ascertaining the area of the hair growth site during the test period, it is possible to ascertain the hair growth effect or the growth period extension effect of the hair growth product. That is, if the hair growth site throughout the test period is large, it indicates that the hair growth effect or growth period extension effect of the hair growth product is large, and conversely, if the hair growth site is small, these effects are small.
【００１２】この発毛部位の面積は、マウスの背部全体
に対する発毛部位の面積の比として把握するのが簡便で
ある。発毛部位の状態を解析して把握することにより、
育毛物の毛の伸長に及ぼす効果の大きさを把握すること
も可能である。The area of the hair growth site is easily grasped as the ratio of the area of the hair growth site to the entire back of the mouse. By analyzing and grasping the state of the hair growth site,
It is also possible to grasp the magnitude of the effect of the hair growth product on hair growth.
【００１３】すなわち、例えば発毛部位の面積を一定と
したとき、短い毛が非常に疎にしか生えていない場合に
は、育毛物の毛の伸長に及ぼす効果が小さく、逆に発毛
部位の面積が狭くても、長い毛が密に生えている場合に
はこの効果が大きいと判断され得る。このようにして、
把握した発毛部位の面積及び状態を数値化して、例えば
両者の数値の積を、例えば発毛評価ポイントとして数値
化して、投与した養毛物を総合的に評価することができ
る。That is, for example, when the area of the hair growth site is fixed, if the short hairs grow very sparsely, the effect on the hair growth of the hair growth product is small, and conversely, the hair growth site has a small effect. Even when the area is small, it can be determined that this effect is large when long hairs are densely grown. In this way,
The area and state of the grasped hair growth site are quantified, and for example, the product of the two values is quantified as, for example, a hair growth evaluation point, and the administered hair restoration can be comprehensively evaluated.
【００１４】（２）発毛維持日数の把握について：発毛
維持日数、すなわち背部皮膚表面上に発毛が認められて
から休止期に入って脱落するまでの日数を把握すること
によって、投与する養毛物の毛周期における成長期の延
長効果を把握することができる。この発毛維持日数の把
握は、例えば発毛部位の一定距離における移動期間を把
握して発毛部位の移動速度を求めて、この移動速度と発
毛部位の平均幅とにより行うことができる。(2) Ascertaining the number of days for maintaining hair growth: Administration is performed by ascertaining the number of days for maintaining hair growth, that is, the number of days from when hair growth is observed on the back skin surface to when the hair enters the rest period and falls off. It is possible to grasp the effect of extending the anagen phase in the hair cycle of the hair growth. The hair growth maintaining days can be grasped, for example, by grasping the movement period of the hair growth site within a certain distance to determine the movement speed of the hair growth site, and using this movement speed and the average width of the hair growth site.
【００１５】この移動速度が速い程、発毛維持日数が短
く、成長期から退行期への移行が早く、逆に遅い程、毛
が成長期に止まる期間が長く、成長期が延長しているこ
とを示すものである。また、さらにマウスの肩のライン
から背中線を引き、そのある一点〔例えば、その背中線
の長さを１としたときの中点（０．５）等〕における発
毛維持期間を求めることにより、それを発毛維持日数と
することができる。このようにして、発毛維持日数を把
握して、投与した養毛物による毛の成長期の延長作用を
検定することができる。[0015] The higher the moving speed, the shorter the hair growth maintenance days, and the earlier the transition from the anagen to the regression period, and conversely, the slower the hair moves, the longer the hair stays in the anagen and the elongation of the anagen. It shows that. Further, a back line is drawn from the shoulder line of the mouse, and a hair growth maintaining period at a certain point (for example, a middle point (0.5) when the length of the back line is 1) is determined. , It can be the number of days of hair growth maintenance. In this way, the number of days for maintaining hair growth can be ascertained, and the effect of the administered hair growth prolongation of the hair growth period can be assayed.
【００１６】[0016]
【実施例】以下、実施例により本発明をより具体的に説
明するが、この実施例により、本発明の技術的範囲が限
定して解釈されるべきものではない。 〔実施例〕 サイクロスポリンＡによる養毛作用の検定 本発明育毛検定方法により、発毛作用が認められている
サイクロスポリンＡの養毛作用を検討した。EXAMPLES Hereinafter, the present invention will be described more specifically with reference to Examples, but it should not be construed that the technical scope of the present invention is limited by these Examples. [Example] Assay of hair-growth effect by cyclosporin A The hair-growth effect of cyclosporin A, which has been shown to have a hair growth effect, was examined by the hair growth assay method of the present invention.
【００１７】試験条件 ヌードマウス： ｂａｌｂ ｃ ｎｕ／ｎｕ 上記マウス性別； 雄 上記マウス週齢： ６週（試験開始時） 上記マウス匹数： ５匹／群 投与薬剤：１％又は５％サイクロスポリンＡエタノー
ル溶液 エタノール（対照） Test conditions Nude mouse: balc nu / nu Sex of the above mouse; Male Age of the above mouse: 6 weeks (at the start of the test) Number of the above mice: 5 / group Drug administered: 1% or 5% cyclosporin A ethanol solution ethanol (control)
【００１８】試験方法 上記ヌードマウスの背部に、各投与薬剤０．１mlずつを
毎日１回塗布した。２〜３日毎に外観発毛状態を写真撮
影により観察した。 Test Method 0.1 ml of each drug was applied to the back of the nude mouse once daily. The appearance of hair growth was observed by photography every 2-3 days.
【００１９】（１）発毛面積比と発毛状態の評価による
発毛評価 発毛面積比の測定 ヌードマウス（以下、単にマウスともいう）の肩のライ
ンから尾までの背中の面積に対する発毛部面積の割合を
算出した。すなわち、サイクロスポリンＡを塗布したマ
ウスの背部において、 式（発毛面積比）＝（発毛部面積）／（背部面積） により、発毛面積比を算出した。(1) Evaluation of Hair Growth by Evaluation of Hair Growth Area Ratio and Hair Growth State Measurement of Hair Growth Area Ratio Hair growth relative to the area of the back from the shoulder line to the tail of a nude mouse (hereinafter also simply referred to as mouse). The ratio of the part area was calculated. That is, on the back of the mouse to which cyclosporin A was applied, the hair growth area ratio was calculated by the formula (hair growth area ratio) = (hair growth area) / (back area).
【００１９】第１図に各群におけるマウスの個体毎の発
毛面積比の経時的変化を示した。また、第２図に各群に
おけるマウスの発毛面積比の平均値の変化を示した。さ
らに、第３図には、各群におけるマウスの発毛面積比の
試験期間中の平均値を示した。FIG. 1 shows the change over time in the hair growth area ratio of each mouse in each group. FIG. 2 shows the change in the average value of the hair growth area ratio of the mice in each group. Further, FIG. 3 shows the average value of the hair growth area ratio of mice in each group during the test period.
【００２０】第１図〜第３図を通じて、用量依存的な養
毛効果が認められているサイクロスポリンＡの投与量に
対して用量依存的に発毛面積比が大きくなっていること
は明らかである。It is apparent from FIGS. 1 to 3 that the hair growth area ratio increases in a dose-dependent manner with respect to the dose of cyclosporin A, for which a dose-dependent hair-growing effect is recognized. It is.
【００２１】特に、第３図において、試験期間中の発毛
面積比の平均がサイクロスポリンＡの用量に依存して増
加していたことにより、試験期間中を通じて発毛部位の
幅がサイクロスポリンＡの用量に依存して増大し、その
結果サイクロスポリンＡの投与により発毛維持期間が延
長することが示された。In particular, in FIG. 3, since the average of the hair growth area ratio during the test period increased depending on the dose of cyclosporin A, the width of the hair growth site throughout the test period was increased. It increased depending on the dose of porin A, and as a result, it was shown that the administration of cyclosporin A prolongs the hair growth maintenance period.
【００２３】よって、上記の試験系で発毛面積比を算出
することにより、ヌードマウスでの発毛効果、特に毛の
成長期が延長したことを示す発毛維持日数の延長をを的
確に判定できることが示された。Thus, by calculating the hair growth area ratio in the above test system, the hair growth effect in nude mice, particularly the extension of the hair growth maintenance days indicating that the hair growth period has been extended, can be accurately determined. It was shown that it could be done.
【００２４】（２）発毛維持日数の評価による成長期延
長の評価 発毛部位の位置の測定及び発毛部幅の算出 マウスの背部の中心線上の肩の位置を０及び尾の位置を
１として、この座標軸上での発毛部先端（尾側）と後端
（首側）との位置を測定し、発毛部幅を（先端の位置）
−（後端の位置）より算出した。(2) Evaluation of prolongation of anagen phase by evaluation of hair growth maintenance days Measurement of hair growth site and calculation of hair growth width The position of the shoulder on the center line of the back of the mouse is 0 and the position of the tail is 1 Measure the position of the hair growth part tip (tail side) and rear end (neck side) on this coordinate axis, and set the hair growth part width (position of the tip)
-(Rear end position).
【００２５】発毛維持期間の算出（１） 発毛部先端が、座標上の０ポイント及び１ポイントを通
過するときの日数（０ポイント：ａ，１ポイント：ｂ）
をそれぞれ求め、「発毛部先端が０ポイントから１ポイ
ントへと移動するのに要する期間」である（ｂ−ａ）及
び「発毛部の移動速度」である〔１／（ｂ−ａ）〕を算
出し、さらに「発毛維持期間」として、（発毛部幅）／
〔１／（ｂ−ａ）〕を算出した。その結果を第１表に示
す。Calculation of Hair Growth Maintenance Period (1) Number of days when the hair growth tip passes through 0 point and 1 point on the coordinates (0 point: a, 1 point: b)
Are obtained, and the “period required for the tip of the hair growth part to move from 0 point to 1 point” is (ba) and the “movement speed of the hair growth part” is [1 / (ba). ] And calculate the “hair growth maintenance period” as (hair growth width) /
[1 / (ba)] was calculated. Table 1 shows the results.
【００２６】[0026]
【表１】 [Table 1]
【００２７】第１表より発毛維持期間は、エタノール投
与群（ＥｔＯＨ）で約２日，１％サイクロスポリンＡ投
与群（１％ ＣｙｓＡ）で約５日という、サイクロスポ
リンＡの養毛効果を的確に示す結果が得られた。よっ
て、この方法によりヌードマウスでの毛の成長期が延長
したことを示す発毛維持期間の延長をを的確に判定でき
ることが示された。From Table 1, the hair growth maintenance period is about 2 days in the ethanol administration group (EtOH) and about 5 days in the 1% cyclosporin A administration group (1% CysA). The result which showed the effect exactly was obtained. Therefore, it was shown that the extension of the hair growth maintenance period, which indicates that the hair growth period in nude mice was extended, could be accurately determined by this method.
【００２８】発毛維持期間の算出（２） 薬剤投与６０日齢付近（発毛サイクルの２サイクル目に
相当する）における発毛部先端及び後端が０．５ポイン
トを通過するときの日数（ｃ，ｄ）を求め、「０．５ポ
イントでの発毛維持期間」である（ｄ−ｃ）を算出し
た。その結果を第２表に示す。Calculation of Hair Growth Maintenance Period (2) The number of days when the leading and trailing ends of the hair growth part pass through 0.5 point around 60 days of drug administration (corresponding to the second cycle of the hair growth cycle). c, d) was calculated, and (dc), which is the “hair growth maintaining period at 0.5 point”, was calculated. Table 2 shows the results.
【００２９】[0029]
【表２】 [Table 2]
【００３０】第２表より、発毛維持期間は、エタノール
投与群（ＥｔＯＨ）では約２日，１％サイクロスポリン
Ａ投与群（１％ ＣｙｓＡ）で約５日，５％サイクロス
ポリンＡ投与群（５％ ＣｙｓＡ）で約７日であり、サ
イクロスポリンＡの濃度に依存して発毛維持期間が延長
し、サイクロスポリンＡの養毛効果を的確に示す結果が
得られた。また、この結果は上記の発毛維持期間の算出
法（１）とほぼ一致していたことから、これらの発毛維
持期間の算出法は両者とも信頼性が認められるものであ
ることが示された。なお、念のために発毛促進効果が既
に認められているレチノール（０．１％）についても、
この系で発毛維持期間を算出したところ、本来レチノー
ルに備わっている発毛促進効果が確認された。As shown in Table 2, the hair growth maintenance period was about 2 days in the ethanol administration group (EtOH) and about 5 days in the 1% cyclosporin A administration group (1% CysA) and 5% cyclosporin A administration. The group (5% CysA) was about 7 days, and the hair growth maintaining period was prolonged depending on the concentration of cyclosporin A, and the result showing the hair growth effect of cyclosporin A accurately was obtained. In addition, since the result was almost consistent with the above-described method for calculating the hair growth maintenance period (1), it was shown that both methods for calculating the hair growth maintenance period were reliable. Was. In addition, retinol (0.1%), for which the hair growth promoting effect has already been recognized, just in case,
When the hair growth maintenance period was calculated in this system, the hair growth promoting effect originally provided in retinol was confirmed.
【００３１】[0031]
【発明の効果】本発明により、養毛剤等の養毛物の簡便
かつ確実な毛周期における成長期の延長効果のインビボ
の検定手段が提供される。Industrial Applicability According to the present invention, there is provided a means for in vivo assay of the effect of prolonging the anagen phase in the hair cycle of a hair-growing product such as a hair-growing agent.
【図１】各群におけるマウスの個体毎の発毛面積比の経
時的変化を示した図である。BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a diagram showing a temporal change in a hair growth area ratio for each mouse individual in each group.
【図２】各群におけるマウスの発毛面積比の平均値の変
化を示した図である。FIG. 2 is a diagram showing changes in the average value of the hair growth area ratio of mice in each group.
【図３】各群におけるマウスの発毛面積比の試験期間中
の平均値を示した図である。FIG. 3 is a view showing the average value of the hair growth area ratio of mice in each group during the test period.
─────────────────────────────────────────────────────
────────────────────────────────────────────────── ───
【手続補正書】[Procedure amendment]
【提出日】平成８年１０月９日[Submission date] October 9, 1996
【手続補正１】[Procedure amendment 1]
【補正対象書類名】明細書[Document name to be amended] Statement
【補正対象項目名】図面の簡単な説明[Correction target item name] Brief description of drawings
【補正方法】変更[Correction method] Change
【補正内容】[Correction contents]
【図面の簡単な説明】[Brief description of the drawings]
【図１】各群におけるマウスの個体毎の発毛面積比の経
時的変化を示した図である。BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a diagram showing a temporal change in a hair growth area ratio for each mouse individual in each group.
【図２】各群におけるマウスの個体毎の発毛面積比の経
時的変化を示した図である。FIG. 2 is a diagram showing a change over time in a hair growth area ratio for each mouse individual in each group.
【図３】各群におけるマウスの発毛面積比の平均値の変
化を示した図である。FIG. 3 is a diagram showing changes in the average value of the hair growth area ratio of mice in each group.
【図４】各群におけるマウスの発毛面積比の試験期間中
の平均値を示した図である。FIG. 4 is a diagram showing the average value of the hair growth area ratio of mice in each group during the test period.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 豊田 英一 東京都品川区西五反田３丁目９番１号 株 式会社資生堂ビューティーサイエンス研究 所内 (72)発明者 田島 正裕 神奈川県横浜市港北区新羽町1050番地 株 式会社資生堂第１リサーチセンター内 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Eiichi Toyoda 3-9-1, Nishigotanda, Shinagawa-ku, Tokyo Inside Shiseido Beauty Science Research Institute (72) Inventor Masahiro Tajima Nippa-cho, Kohoku-ku, Yokohama, Kanagawa Prefecture 1050, Shiseido Daiichi Research Center Co., Ltd.
Claims (10)
ドマウスの体表の発毛部位の状態を特定して、育毛物の
毛周期における成長期を延長する効果を検定する育毛検
定方法。A hair growth assay method comprising administering a hair growth product to a nude mouse, specifying the state of the hair growth site on the body surface of the nude mouse, and testing the effect of extending the growth period in the hair cycle of the hair growth product.
が、その体表に経時的にその発毛部位が移動する特徴的
な発毛をするヌードマウスである請求項１記載の育毛検
定方法。2. The hair growth method according to claim 1, wherein the nude mouse is hairless in principle, but is hairless in principle, but has a characteristic hair growth in which the hair growth site moves over time on the body surface. Test method.
ｕである請求項１又は請求項２記載の育毛検定方法。3. The method according to claim 1, wherein the nude mouse is bal c nu / n.
3. The method for assaying hair growth according to claim 1, wherein the method is u.
定手段が、発毛部位の面積及び／又は状態の把握ある請
求項１乃至請求項３のいずれかの請求項記載の育毛検定
方法。4. The hair growth assay according to any one of claims 1 to 3, wherein the means for specifying the state of the hair growth site on the body surface of the nude mouse comprises grasping the area and / or state of the hair growth site. Method.
び／又は発毛密度の把握である請求項４記載の育毛検定
方法。5. The hair growth assay method according to claim 4, wherein the means for grasping the condition of the hair growth site is grasping the length and / or density of hair.
定手段が、発毛部位の発毛維持日数の把握である請求項
１乃至請求項３のいずれかの請求項記載の育毛検定方
法。6. The hair growth assay according to any one of claims 1 to 3, wherein the means for specifying the state of the hair growth site on the body surface of the nude mouse is grasping the number of days of hair growth maintained at the hair growth site. Method.
毛部位の一定距離における移動期間を把握して発毛部位
の移動速度を求めて、この移動速度と発毛部位の平均幅
を指標とする方式である請求項６記載の育毛検定方法。7. A means for grasping the number of days of hair growth of a hair growth site, wherein the movement period of the hair growth site at a certain distance is grasped to determine the movement speed of the hair growth site. 7. The method for testing hair growth according to claim 6, wherein the method uses a width as an index.
ウスの背部の一点における発毛維持期間を指標とする方
式である請求項６記載の育毛検定方法。8. The method for assaying hair growth according to claim 6, wherein the means for determining the number of days of hair growth maintained at the hair growth site is a system using the hair growth maintenance period at one point on the back of the mouse as an index.
ンからの背中線の一点である請求項８記載の育毛検定方
法。9. The hair growth assay method according to claim 8, wherein one point on the back of the mouse is one point on the back line from the shoulder line of the mouse.
が、その背中線の中点である請求項９記載の育毛検定方
法。10. The hair growth assay method according to claim 9, wherein one point of the back line from the shoulder line of the mouse is the midpoint of the back line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22779696A JP3582940B2 (en) | 1996-08-11 | 1996-08-11 | Hair growth test method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22779696A JP3582940B2 (en) | 1996-08-11 | 1996-08-11 | Hair growth test method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH1052405A true JPH1052405A (en) | 1998-02-24 |
| JP3582940B2 JP3582940B2 (en) | 2004-10-27 |
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ID=16866532
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22779696A Expired - Fee Related JP3582940B2 (en) | 1996-08-11 | 1996-08-11 | Hair growth test method |
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| Country | Link |
|---|---|
| JP (1) | JP3582940B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10265341A (en) * | 1997-03-26 | 1998-10-06 | Shiseido Co Ltd | Assay of hair-glowing agent |
| JPWO2008102602A1 (en) * | 2007-02-22 | 2010-05-27 | 国立大学法人 東京大学 | Organ regeneration method using BLASTOCYSTCOMPLEMENTATION |
| JPWO2010021390A1 (en) * | 2008-08-22 | 2012-01-26 | 国立大学法人 東京大学 | Organ regeneration method using iPS cells and BLASTOCYSTCOMPLEMENTATION |
-
1996
- 1996-08-11 JP JP22779696A patent/JP3582940B2/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10265341A (en) * | 1997-03-26 | 1998-10-06 | Shiseido Co Ltd | Assay of hair-glowing agent |
| JPWO2008102602A1 (en) * | 2007-02-22 | 2010-05-27 | 国立大学法人 東京大学 | Organ regeneration method using BLASTOCYSTCOMPLEMENTATION |
| JP2015061525A (en) * | 2007-02-22 | 2015-04-02 | 国立大学法人 東京大学大学 | Organ regeneration method utilizing blastocyst complementation |
| JP2016195605A (en) * | 2007-02-22 | 2016-11-24 | 国立大学法人 東京大学 | Organ regeneration process using blastocyst complementation |
| JP2018138048A (en) * | 2007-02-22 | 2018-09-06 | 国立大学法人 東京大学 | Organ regeneration method utilizing blastocyst complementation |
| JP2020078327A (en) * | 2007-02-22 | 2020-05-28 | 国立大学法人 東京大学 | Organ regeneration method using BLASTOCYST COMPLEMENTATION |
| JPWO2010021390A1 (en) * | 2008-08-22 | 2012-01-26 | 国立大学法人 東京大学 | Organ regeneration method using iPS cells and BLASTOCYSTCOMPLEMENTATION |
| JP2014230552A (en) * | 2008-08-22 | 2014-12-11 | 国立大学法人 東京大学 | ORGAN REGENERATION METHOD UTILIZING iPS CELL AND BLASTOCYST COMPLEMENTATION |
| JP5688800B2 (en) * | 2008-08-22 | 2015-03-25 | 国立大学法人 東京大学 | Organ regeneration method using iPS cells and BLASTOCYSTCOMPLEMENTATION |
| JP2017018133A (en) * | 2008-08-22 | 2017-01-26 | 国立大学法人 東京大学 | Organ regeneration method using ips cells and blastocyst complementation |
| JP2019030310A (en) * | 2008-08-22 | 2019-02-28 | 国立大学法人 東京大学 | Organ regeneration method using iPS cells and BLASTOCYST COMPLEMENTATION |
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