JPH10248556A - Microorganism for deodorization - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a deodorant capable of deodorizing a malodor, especially a sulfur-based malodor by containing a specific microorganism as an active ingredient. SOLUTION: This deodorant contains a microorganism having a deodorizing activity and belonging to the genus Pseudomonas, e.g. Pseudomonas sp. DMS-1 strain (FERM-P-16138) or Pseudomonas sp. MD-1 strain (FERM-P-16139) as an active ingredient. The DMS-1 and MD-1 strains are Gram negative bacilli, have motilities, positive catalase and oxidase activities, and capacities of using maltose, xylose, etc., as substrates, and grow at pH in the vicinity of neutral region. The DMS-1 has an activity to decompose methylsulfide, and the MD-1 has an activity to decompose methyldisulfide.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a microorganism for deodorizing and a deodorant using the same.

[0002]

2. Description of the Related Art It is desirable that malodors be removed from the environment more quickly because they not only cause discomfort to humans but also actually harm the human body. Current deodorizing methods include a masking method, an adsorption method, a chemical treatment method, and a combustion method. These methods all exhibit a certain degree of deodorizing effect, but have the following problems.

[0003] The masking method is a method of masking a bad smell with a stronger odor (fragrance), as represented by a fragrance of a toilet. Although this method is inexpensive, it does not reduce the odor itself. The adsorption method is a method in which an odor is taken into a porous body such as zeolite. Unlike the masking method, this method can reduce the bad odor itself, but has a limit on the amount of the bad odor to be taken in, and if it exceeds that, the adsorbent must be replaced, which is disadvantageous in cost and the like. The chemical treatment method is a method of decomposing a bad smell using an acidic or alkaline chemical. This method has problems with running costs and disposal of chemicals after use. The combustion method is a method of heat treating a bad odor. Although this method has a high odor treatment capacity, it has a problem in running cost. As described above, each of the deodorizing methods currently performed has a specific problem, and development of a more excellent deodorizing method is awaited.

[0004]

In addition to the above physical and chemical deodorizing methods, there is also a method of decomposing odorous substances by using microorganisms. This method has the advantages of low running cost and easy management, but the method currently in practical use does not always provide a satisfactory deodorizing effect. The reason is that the microorganism to be used has not been sufficiently studied, and an unspecified microorganism has been used empirically. In particular, research on the relationship between sulfur-based malodorous components and microorganisms has hardly been advanced, and microorganisms that can be used for deodorization of sulfur-based malodor include microorganisms of the genus Thiobacillus (Thiobacillus) and microorganisms of the genus Hyphomonas. Only the species are known. Moreover, the growth and pH of the microorganisms of the genus Thiobacillus are limited to 2 to 3 so that cultivation and maintenance are limited. Among the components, only hydrogen sulfide and methyl mercaptan are included. An object of the present invention is to solve the problem of deodorization of such malodors caused by microorganisms, particularly sulfur-based malodors.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, they have been able to grow in microorganisms belonging to the genus Pseudomonas at a pH around neutrality. In addition, the present inventors have found a microorganism having the ability to degrade hardly decomposable methyl sulfide and methyl disulfide, and completed the present invention based on this finding.

That is, the present invention is a deodorant comprising a microorganism belonging to the genus Pseudomonas and having a deodorizing ability as an active ingredient. The present invention also relates to a Pseudomonas sp. DMS-1 strain having the ability to degrade methyl sulfide. Furthermore, the present invention is a Pseudomonas sp. MD-1 strain having a methyl disulfide decomposability.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
First, the strain of the present invention will be described. Pseudomonas sp. Strain DMS-1 is a strain isolated from the soil of Okutama-cho, Tokyo, and Pseudomonas sp. Strain MD-1 is a strain isolated from the soil of Kunigami-son, Okinawa Prefecture. These strains have the following mycological properties.

DMS-1 MD-1 Morphology Rod Size 1.2 × 0.3 1.0 × 0.3 Gram staining − − Acid-fast − − Spore − − Motility (+) (+) Proliferation under aerobic condition + + Colony morphology ( Rough cream Rough cream Growth temperature 5 ° C--10 ° C--20 ° C + + 30 ° C + + 40 ° C + + 50 ° C--Growing under anaerobic conditions--Growing pH 3.0--6.0 + + 7.0 + + 8.0 + + Catalase + + Oxidase + + DNase--Cellulase--Urease--OF test O O Gelatin liquefaction--Hydrogen sulfide production--Indole--IPA production--Litmus milk alkali alkali nitrate reduction + + Β-galactosidase − − phogesprose cauer − − phosphatase − − citrate ++ hippurate ++ arginine ++ esculin ++ --Leucine + + Salicin--ONPG +-Production of acid from sugar Adonitol--Arabinose--Cellobiose--Darcitol--Erythritol--Fructose + + Galactose--Glucose + + Glycerol--Inositol--Lactose --Maltose ++ Mannitol--Mannose-+ Mantoose--Melviose--Raffinose--Rhamnose--Sorbitol--Sorbose--Sucrose--Trehalose--Xylose +++: Positive,-: Negative, (+) ： Weak positive

[0009] The above mycological properties can be determined using the Systematic Bacteriorogy vol.1 of the Burgess Manual.
As a result of study with reference to (1984), DMS-1 and MD-1 are Gram-negative bacilli, having motility, catalase positive, oxidase positive, and assimilation ability of various carbon sources,
Both strains were determined to belong to the genus Pseudomonas. The Pseudomonas sp. DMS-1 strain was designated as FERM P-16138 (deposit date: March 13, 1997), and Pseudomonas sp.
The MD-1 strain was designated as FERM P-16139 (Deposit date: March 1997)
13th), deposited at the Institute of Biotechnology and Industrial Technology, respectively.

The strain of the present invention can be cultured according to a culture method generally applied to microorganisms belonging to the genus Pseudomonas. The medium includes assimilable carbon sources, nitrogen sources,
Any medium may be used as long as the medium contains inorganic salts and the like. As a carbon source, glucose, fructose, maltose and the like can be used, and as a nitrogen source,
Yeast extract, meat extract, peptone, and the like can be used, and may further contain, as inorganic salts, sodium chloride, calcium chloride, potassium phosphate, and the like. The culture method is preferably a liquid culture method under aerobic conditions, the temperature during the culture is preferably 20 to 40 ° C, and the pH is preferably 6 to 8.

Next, the deodorant of the present invention will be described.
The deodorant of the present invention contains a microorganism belonging to the genus Pseudomonas as an active ingredient. The microorganism used here is not particularly limited as long as it has a deodorizing ability,
Those having a deodorizing ability with respect to sulfur-based malodors are preferable, and those having a deodorizing ability with respect to malodors caused by methyl sulfide and methyl disulfide are more preferable. Specific strains include the above Pseudomonas sp. DMS-1 strain and Pseudomonas sp.
sp. MD-1 strain or mutants of these strains can be exemplified.

The deodorant of the present invention can take any form as long as the microorganism can exert a deodorizing effect. For example, the cells of the microorganisms themselves may be used as a deodorant, or a suspension of the cells in water or the like or a culture solution of the microorganisms may be used as a deodorant. It may be mixed with an additive to form a deodorant.

The deodorant of the present invention can be used, for example, for deodorizing livestock manure, sewage sludge, household garbage, food residues, and the like during composting. Can also be used. The amount of the deodorant used at this time may be determined according to the type and amount of the deodorizing object.

[0014]

【Example】

[Example 1] Separation of microorganisms Soil was collected from four areas: Okutama-cho, Tokyo, Ome-shi, Tokyo (in the livestock experimental station), Iriomote-jima, Okinawa, and Kunigami-son, Okinawa (northern Okinawa), and contained malodorous components. They were placed on a complete synthetic medium (Table 1) and cultured at 30 ° C. for 24-48 hours. After completion of the culture, the microorganisms that could grow in the medium were separated.

Each of the separated microorganisms is grown in a heart infusion broth (HIB, Table 2), and the cells are collected by centrifugation.
× 10 ⁸ cells / ml). 15 ml of the medium containing the cells was cultured with shaking at 30 ° C. for 48 hours in a sealed test tube. After cultivation, gas chromatography of methyl sulfide or methyl disulfide in the liquid phase (G5000, manufactured by Hitachi, Ltd.)
Was measured. Strains in which the amount of methyl sulfide or methyl disulfide was significantly reduced as compared to the control group (without addition of microorganisms) were selected. As a result, strain DMS-1 was selected as a methyl sulfide-utilizing bacterium and strain MD-1 was selected as a methyl disulfide-utilizing bacterium.

[0016]

[Table 1]

[0017]

[Table 2]

Example 2 Measurement of Methyl Sulfide Degradation The DMS-1 strain was cultured with shaking at 30 ° C. for 48 hours in a heart infusion broth (described above), and cells were recovered from the culture by centrifugation. The obtained cells were cultured at a constant concentration (2.0 × 10 ⁸ cells)
/ ml). 15 ml of this medium was shake-cultured at 30 ° C. for 48 hours in a sealed test tube, and the concentration of methyl sulfide in the liquid phase after the culture was measured by gas chromatography (G50, manufactured by Hitachi, Ltd.).
00). Further, as a control, the concentration of methyl sulfide when no microorganism was added was also measured. The measurement was performed three times,
An average measurement was calculated. Table 2 shows the results.

[0019]

[Table 3]

As shown in the above table, the concentration of methyl sulfide after culturing when the DMS-1 strain was added was 30 ± 3 ppm, which was significantly (p <0.05) compared to the methyl sulfide concentration of the control group of 43 ± 6 ppm. From these results, it was determined that the DMS-1 strain had the ability to decompose methyl sulfide. In addition, when the decomposition rate of methyl sulfide is applied to the following formula, there is about 30% resolution under this condition.

[0021]

Example 3 Measurement of Degradability of Methyl Disulfide Same as Example 2 except that strain MD-1 was used instead of strain DMS-1 and that methyl disulfide was used instead of methyl sulfide. Then, the concentration of methyl disulfide was measured. Table 3 shows the results.

[0023]

[Table 4]

As shown in the above table, the concentration of methyl disulfide after culturing when the MD-1 strain was added was 32 ± 5 ppm, which was significantly higher than the concentration of methyl disulfide in the control plot (46 ± 6 ppm).
<0.05), indicating that the MD-1 strain has the ability to decompose methyl disulfide. In addition, when the decomposition rate of methyl disulfide is applied to the above formula, under this condition,
There will be 30% resolution.

[0025]

The present invention provides a novel deodorant utilizing microorganisms. ADVANTAGE OF THE INVENTION The deodorant of this invention can remove | eliminate the sulfur-based malodor which is difficult to deodorize efficiently.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI // (C12N 1/20 C12R 1:38)

Claims (3)

[Claims] 1. A deodorant comprising, as an active ingredient, a microorganism belonging to the genus Pseudomonas and having a deodorizing ability. 2. A Pseudomonas sp. DMS-1 strain having a methyl sulfide decomposing ability. 3. A Pseudomonas sp. MD-1 strain having a methyl disulfide decomposability.