JPH10203993A - Genuinely natural hypoglycemic agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject hypoglycemic agent containing Sekirenka or an extract therefrom. SOLUTION: This hypoglycemic agent contains, as the main ingredient, Sekirenka belonging to the genus Keitenka (a kind of bush which naturally grows on the rocky mountain in China) or an extract therefrom. The extraction is carried out by the following process: pref. both the stems and leaves of Sekirenka are ground so as to destroy the cell wall of Sekirenka followed by extraction using a solvent such as water or an alcohol. The resulting extract (liquid) is concentrated and, as necessary, heated into powder; or, the concentrate may be purified and used. The daily dose of this agent is as follows: when the ground product of Sekirenka is to be taken directly, 3-4g/adult; while, in the form of raw powder afforded by extraction and concentration, 50-70mg/adult. This agent is useful for treating diabetes, also being effective when formulated in foods or drinks.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a blood sugar lowering agent, and more particularly to a pure natural blood sugar lowering agent containing a pure natural ingredient as a main component.

[0002]

2. Description of the Related Art It is well known that diabetes is one of adult diseases and is one of the extremely difficult diseases to be completely cured or treated.

[0003] Conventionally, various drugs have been developed as antidiabetic drugs and hypoglycemic drugs, but their effects are insufficient, and in view of their side effects, those produced from natural products have been developed. It is highly desirable and there is a strong need for the development of effective drugs made from pure natural products.

[0004] More specifically, a number of new drugs for the treatment of non-insulin-dependent diabetes mellitus and herbal medicines have been invented in Japan and overseas. There are drawbacks, such as low blood sugar, even if the effect is good. A nutrient-type blood glucose lowering agent that is pure natural, highly effective, safe without toxic side effects, and can be adjusted to a normal value from both high and low blood sugar levels is expected.

[0005]

SUMMARY OF THE INVENTION The present invention aims to provide a new drug which is effective against diabetes, ie, a pure natural drug which can lower blood sugar level.

[0006]

This problem is solved by using stone lotus or an extract thereof as a hypoglycemic agent.

[0007]

According to the study of the present inventor, it has been found that the natural plant Sekiren contains a component that lowers the blood sugar level. , Blood sugar levels were found to drop. For example, acute toxicity experiments in mice proved non-toxic, and when a mouse model of diabetes was established and tested, it was found that blood glucose levels were significantly reduced. In addition, as a result of conducting a teratogenicity experiment on rats, it was also found that there was no teratogenic effect. In addition, it has been found from an actual clinical experiment that it also has a blood sugar lowering effect on humans. The present invention has been completed based on these new discoveries. In addition, in the present invention, not only the extract component of the lotus flower but also the lotus flower itself has a blood sugar lowering action.

The lotus flower used in the present invention belongs to Jingtianae, and is also referred to as Jingtianae Shenren (Chinese Botanical Journal, Vol. 34, 1st volume, 63rd page) Ex. Ba-k, Vol. 1, pp. 575-576;
er. in Sound. Refug. Bot. 1: s
ub T. 61.1863-Coiledon gl
auca Baker). Stone lotus is semi-shrub-like or herbaceous, about 60 cm tall, with thick roots, semi-erect, and loose spiral scars densely covered. Many elongated air roots are formed, a new lotus group often appears beside the leaf scar, and when the lotus group grows, a new rhizome emerges from below and becomes a parting branch of the old stem. The leaves are indigo-gray, and set in lotus groups. Leaf pieces are slightly fleshy, inverted spoon-like, 5-8 cm long, maximum width 2.
It is 5-4 cm, circular with a widened tip, with a small point at the center, about 1 mm. Red, base converges into a spoon. The length is about 1/3 to 1/2 of the leaf length. The stem of the flower emerges from the side and is 20-30 height
cm, flower 10-20 cm, single umbrella inflorescence; flower crown is pink on the outside, yellow on the inside, 1.5 cm in length, tip slightly spreads out. The flowering period is from June to August.

According to the literature, the stone lotus is native to Mexico, and according to the inventor's research on the place of origin, the stone lotus grows naturally on a rocky mountain in the mountains of Bama Prefecture, Guangxi Province. From a distance, the pink flowers with stone lotus flowers look beautiful. This stone lotus flower is known in botany as a plant completely different from the Kagetenshin swallow palm.

In the present invention, it is particularly preferable to use the leaves and stems of the stone lotus. That is, the leaves and stems themselves, their crushed products, or the components extracted by these extraction solvents are particularly preferable because of their high blood sugar lowering effect.

The method of extracting the extracted components from the stone lotus flower is as follows. First, in the present invention, the cell wall of the stone lotus flower is destroyed, and then the active ingredient is extracted using a suitable extraction solvent. The extract is concentrated and, if necessary, heated to a powder. In some cases, the concentrate is purified. These will be described in more detail below.

Means for breaking the cell wall of the stone lotus flower include means for crushing the stone lotus flower and then immersing it in alcohol, means using ultrasonic waves, rapid freezing and thawing means, particularly preferably flash freezing and thawing means. One or two of these means
This is done in more than one way. Alcohol immersion means is usually immersed in medicinal alcohol (eg, 98% medicinal alcohol) or diluent (eg, diluent up to about 70%) at room temperature for 10 to 10 minutes.
Soak for about 24 hours. At this time, the temperature may be slightly increased, but it is preferably performed at 60 ° C. or lower.

In the case of using ultrasonic waves, appropriate ultrasonic waves, for example, 7
An ultrasonic wave of about 100 to 1000 HZ is irradiated for about 1 to 10 minutes.

The rapid freezing and thawing means is preferably as quick as possible, preferably instantaneously frozen, and thawed as soon as possible. The freezing temperature is about −10 ° C. or less, preferably −
About 20 ° C may be sufficient.

As described above, the stone lotus flower is crushed and the cell wall is destroyed, and then extracted using an extract. Examples of the extract at this time include water, alcohol, and an aqueous alcohol solution as typical examples. In this case, medicinal alcohol is usually used. The extracted components are concentrated and evaporated to a powder. This powder can be used as it is as a blood glucose lowering agent.

The above concentrate may be purified by a silica gel column or by a gradient elution using CH ₃ OH on a Saphade-x LH column. Also, the concentrated solution was CH ₃ COOC ₂ H ₅
After extraction, wash the precipitate repeatedly with CH ₃ OH. CH ₃
A method of recovering OH, further purifying the precipitate by washing with CH ₃ OH and then precipitating is also employed. An example of a specific extraction method will be described below as an experimental example.

[0017]

EXPERIMENTAL EXAMPLE 1 As shown in FIG. 1, a plant cell wall is broken with 50-70% medicinal alcohol. That is, after washing the leaf or stem of fresh stone lotus flower and pulverizing it with a pulverizer, 4 liters of 98%
Add medicinal alcohol, adjust the specific gravity of the aqueous solution to 70% with water or the water of the lotus flower, and soak overnight at room temperature. Filter the next day and collect the filtrate. 4 liters of distilled water was added to the residue, and the above operation was repeated 4 times. The filtrates obtained 5 times were combined and concentrated under reduced pressure in a water bath at 60 to 70 ° C. to give a concentration of 50 g.
A 200 ml / ml concentrate is obtained.

[0018]

[Experimental example 2] As shown in Fig. 1, the plant cell wall is broken by flash freezing and thawing. That is, leaves or stems of fresh stone lotus flowers (for example, 10
kg) after washing, pulverizing with a pulverizer, instantaneously freezing to -20 ° C, maintaining the same temperature for 4 hours, instantaneously heating and thawing to 40 to 50 ° C, and then distilled water (for example, 4 liters).
And soak overnight. Filter the next day and collect the filtrate. Distilled water (for example, 4 liters) is added to the residue, and the above operation is repeated 4 times, and the filtrate obtained 5 times is combined with 60 to 7 times.
The solution was concentrated under reduced pressure in a water bath at 0 ° C. to obtain a concentrated solution 2 having a concentration of 50 g / ml.
00 ml are obtained.

[0019]

EXPERIMENTAL EXAMPLE 3 As shown in FIG. 1, a plant cell wall is broken by an ultrasonic cell crusher. That is, leaves or stems of fresh stone lotus flowers (for example, 1
0kg) after washing, pulverizing with a pulverizer and then W220 type S
800-9 using ONIGATOR ultrasonic cell crusher
Stir at 00HZ for 5 minutes, then add 400 ml of distilled water and soak overnight. Filter the next day and collect the filtrate. Distilled water (4 liters) was added to the residue, and the above operation was repeated four times. The filtrate obtained five times was combined, concentrated under reduced pressure in a water bath at 60 to 70 ° C., and 200 ml of a concentrated solution having a concentration of 5 g / ml was obtained.
Is obtained.

[0020]

[Experimental example 4] The concentrated liquid 20 of the concentration of 50 g / ml of Experimental example 1
0 ml is evaporated to obtain 243 g of raw powder. The raw powder acquisition rate from fresh stone lotus is 2.43%.

[0021]

EXPERIMENTAL EXAMPLE 5 Concentrated liquid 20 having a concentration of 50 g / ml of Experimental Example 2
0 ml is processed according to the flowchart of FIG. 2, and 239 g of raw powder is obtained. The acquisition rate is 2.39%.

[0022]

[Experimental Example 6] The concentrated liquid 200 having a concentration of 5 g / ml of Experimental Example 3
ml is processed according to the flow chart of FIG. 2 to obtain 23.6 g of raw powder. The acquisition rate is 2.36%.

[0023]

EXPERIMENTAL EXAMPLE 7 A 50 ml portion of the concentrated solution of Experimental Example 1 was taken and subjected to a silica gel column (100 to 150 mesh, 3 × 36).
In step 0), the operation is performed in accordance with the fine powder production process shown in FIG. That is, CH ₃ COOC ₂ H ₅ , CHCI ₃ , CH ₃ COCH ₃ ,
And CH ₃ COOC ₂ H ₅ and CH ₃ COCH ₃ 1: 1 _{mixture, CH 3 OH, CH 3 COCH} 3 and of CH ₃ OH 5: 1 mixture, CH ₃ COCH ₃ and of CH ₃ OH 1: 1 mixture 7
CH ₃ COCH ₃ and CH ₃ collected at peak values observed with an ultraviolet observer
The components de-eluted with a 5: 1 mixture with OH are separated on a silica gel thin layer (GF254 10-40μ).
4: 0. Of CH ₃ COOC ₂ H ₅ , CH ₃ OH and H ₂ O.
6: 0.3 mixture, Rf on thin layer: 0.37 ~
The components in the 0.52 part were collected and CH ₃ COCH ₃ and CH
Dissolve in a 5: 1 mixture with ₃ OH, filter, evaporate in vacuo,
17.01 g of refined powder was obtained. The rate of obtaining flour from fresh stone lotus flower is 28%.

[0024]

[Experimental example 8] 50 m of the concentrated liquid of the concentration of 5 g / ml of Experimental example 3
1 is processed in the production process of FIG. That is, Saphade
x LH-20 column (50 × 1200) CH ₃ OH
Perform Crude Elution with. The active ingredient eluted at the peak value of the ultraviolet observer was collected, filtered, evaporated under reduced pressure, and 0.974 g of fine powder was obtained. Acquisition rate is 1
6.5%.

[0025]

EXPERIMENTAL EXAMPLE 9 200 ml of the concentrated solution of Experimental Example 2 was
Extract 4 times with 0 ml of CH ₃ COOC ₂ H ₅ and extract 4 times CH ₃
Combine the COOC ₂ H ₅ solution. The aqueous solution is concentrated under reduced pressure,
The precipitate is washed repeatedly with CH ₃ OH and then CH ₃ O
Collect H. The precipitate is then repeatedly precipitated with anhydrous alcohol and the alcohol is recovered under reduced pressure. 89.6 g of refined powder are obtained. The acquisition rate is 37.5%. Table 1 shows the components extracted from the stone lotus flower of the present invention.

[0026]

[Table 1]

[0027]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments will be described below in detail.

[0028]

Example 1 Acute toxicity experiment of the hypoglycemic agent of the present invention. It is shown in Table 2. However, in Table 2, the maximum withstand load (Maximu
m tolle-d dose, abbreviated as MTD).

[0029]

[Table 2]

[0030]

Example 2 Blood glucose lowering experiment of raw powder and sperm powder (1) Experimental method 88 Kunming mice were randomly selected as a control group, DSP, DA
S, DAS ₁ , DAS ₂ , DAS ₃ , damon, and dominant sugar were divided into eight sets, each set consisting of 11 female mice. Tetraoxid
icpyrim-idine) to make a diabetes model,
A comparison is made between commercially available DSP, Damicron and dominant glucose as reference samples for lowering blood glucose levels. The dose of each set is 0.5 mg / ml in raw powder concentration and 0.2 ml per animal every day.
The control group receives only 0.2 ml of physiological saline daily. Six days after administration (put into the stomach), blood is collected and the blood glucose level is measured.

(2) Experimental Results Biological statistics were performed on the measured data, and the results are shown in Table 3.

[0032]

[Table 3]

In Table 3, DAS is the raw powder of the lotus flower obtained in Experimental Example 4, and DAS ₁ , DAS ₂ and DAS ₃ are the raw powder of the lotus flower obtained by the methods of Experimental Examples 7, 8, and 9, respectively. Fine powder.

The following is clear from Table 3. (B) The order of the blood sugar level drop rate is DAS ₂ > DAS ₃ >
DAS ₁ >DSP> Doubly Sugar> Damicron, especially D
AS ₂ has the best blood glucose lowering effect. (B) DAS ₂ has a very significant difference, and each of the other experimental sets also has a significant difference. (C) The control group has no statistical significance.

[0035]

Example 3 Teratogenicity Experiment (1) Experimental Animal An experiment is performed using a Wistar rat, 3 months old, which is a first-grade animal provided by the Chinese Academy of Medical Science Animal Department. Animal experiments are performed after 4 days in the laboratory. Room temperature of animal room is 25 ± 1
In ° C., the humidity is 60-80%.

(2) Dose Classification The maximum dose group (200 mg / kg) was 200 times the dose used in the clinical experiment (1 mg of raw powder per kg of body weight daily).
kg) at 50% / kg, 1
Two dose sets of 2.5 mg / kg are provided. In addition, a positive control group (Acet-yl salicylic acid)
d200 mg / kg) and a negative control set are provided for comparison. The total number of rats is 5 and the number of rats crossed is 14-1.
There are five.

(3) Administration Method and Administration Time The raw powder solution and an 8.3% acetyl salicylic acid solution are administered intragastrically. Administration is performed for 9 consecutive days from the 6th to 14th day after fertilization.

(4) Experimental Method Female and male rats are mated at 6:00 pm every day in the same room at a ratio of 2: 1. At 8:30 a.m. the next day, examination of the yin and sperm is performed. Assuming that spermatozoa have been found, the day is termed fertilization 0. At this time, the body weight is measured, randomly placed in each experimental group, and weighed on the fifth, tenth, fifteenth, and twenty days after fertilization. On the 20th day, they were killed by cervical dislocation, the wombs were removed, and their appearance was routinely examined.
A skeletal malformation test is performed by the rin c-rimson staining method, and a visceral malformation test is performed by the Bordeaux solution fixation method.

(5) Experimental Results Table 4 shows the results.

[0040]

[Table 4]

(6) Conclusion According to the above results, the experimental set is equivalent to the human clinical dose of 12.
There is no significant difference (P> 0.05) compared to the negative control group even at 5, 50 and 200 times. Therefore, it is considered that rats have no teratogenic effect.

[0042]

Example 4 Collision (mutation) test (1) Experimental method a. Ames experiment Salmons bacteria nutritionally incomplete type TA97, TA98, TA10
0 and TA102 were used for direct invasiveness experiments using a plate-entry method, and mouse Micromease.
An indirect aggressiveness experiment is performed using the of river (S9). The strain was donated by the Ames Laboratory of the University of California, USA, and was confirmed to be acceptable by biological analysis. In this experiment, four dose sets of 5,50,500,1000 μg / dish are provided, and a positive control set and a negative control set are additionally provided (natural regression). For one experiment, parallel experiments are performed with three samples, and two experiments are performed. Display the result as an average. A bacterial recovery variable that exceeds twice the natural recovery variable is judged to be abruptly positive.

B. Mouse bone marrow micronucleus experiment The experiment is carried out using Kunming mice (first-grade animals provided by the Animal Laboratory of this hospital). Mice weigh 24-30 g.
In this experiment, three dose groups of 10,600,1200 mg / kg body weight were provided, and a positive control group (Cycloph) was additionally provided.
osphami-de, 30 mg / kg, injected twice in the abdomen) and a negative control set. Each set consists of 10 female and 5 male mice each. The administration method was gastric injection, and administration was continued for two consecutive days. Six hours after the last administration, the mice were killed, the bone marrow of the sternum was taken out, sliced by a conventional method, and stained. For one mouse, 1000 permissive polydye cells (very easy to dye red) are given by Cou.
The number of micronucleated cells is calculated by observing the red cells containing micronuclei while counting.

C. Mouse Sperm Mutation Experiment An experiment is performed using Kunming mice (first-class animals provided by the Animal Center of the Academy of Military and Medical Sciences). The weight of the mouse is 27-3
6 g. Three dose groups of 10,600,1200 mg / kg were provided, and another positive control group (Cycloph
osphami-de, 30 mg / kg, injected in the abdomen for 5 consecutive days) and a negative control set. The stomach is continuously injected for 5 days, and the animal is killed 35 days after the first administration. The bilateral testicles are removed, sliced and stained. 10 per mouse
Observe 00 spermatozoa and calculate the sperm mutation rate.

(2) Experimental results a. Ames experiment: Table 5 shows the results. As can be seen from Table 5, the spontaneous recovery numbers of the four strains are within the set range regardless of whether S9 is added or not. The number of recovered bacteria of each positive substance is all three times or more than the corresponding natural recovery number. For four dose sets, S9
The number of recovered bacteria was less than twice the number of spontaneously recovered, regardless of whether or not to increase the number of cells. This indicates that the hypoglycemic agent of the present invention was effective against four strains of mouse wound cold Sammons nutrient-deficient in the experimental dose range. Does not cause direct or indirect mutagenesis. However, Table 5 shows A
The results of the mes experiment (recovered bacterial count / dish M ± SD) are shown.

[0046]

[Table 5]

B. Mouse bone marrow micronucleus test: Experimental results are shown in Table 6.
(Results of the micronucleus test).

[0048]

[Table 6]

As a result of a ² statistical analysis, a very remarkable difference is observed between the positive control group and the negative control group (P <
0.01). The three dose sets show no significant difference (P> 0.05) compared to the negative control set. These facts indicate that the depressant of the present invention has no effect of inducing an increase in the rate of micronuclei in the bone marrow hyperplasia of mice in the above experimental dose range.

C. Mouse sperm mutation experiment Table 7 shows the results of mouse sperm mutation experiment.

[0051]

[Table 7]

As a result of statistical processing, a very remarkable difference was observed between the positive control group and the negative control group (P <0.0).
1) There is no significant difference between the three dose groups compared to the negative control group (P> 0.05). Therefore, the hypoglycemic agent of the present invention has no effect of inducing sperm mutation in mice within the above experimental dose range.

The conclusion of the above three experimental results is that no mutation was observed within the experimental dose range, and that the hypoglycemic agent of the present invention caused mutations in somatic and germ cells of mice. There seems to be no.

[0054]

Example 5 Subacute Experiment (1) Experimental Animals Wistar weanling rats, weighing 210-220 g, are grade 1 animals of the Experimental Animal Center of the Chinese Academy of Medical Sciences.

(2) Animal grouping and dose The animals were randomly divided into four groups, each group consisting of 24 animals, each male and female half. Divide into six experimental sets and one control set. The experimental set is divided into three dose sets: high, medium and low. The low, medium and high dose groups are 10 mg / kg, 50 mg / kg, 1 equivalent to 10 times, 50 times and 100 times the human daily dose (1 mg / kg).
00 mg / kg.

(3) Observation items Table 8 shows the observation items. However, Table 8 shows the P value of the measurement results (X ± SP) of the serum biochemical index on the 45th day. From Table 8, all the P values are> 0.05, which is not different from the control group. Also, the control set has no statistical significance.

[0057]

[Table 8]

Hb: serum protein WBC + DC: total number and classification of white cells S-GPT: transferase TG: glyceride CHO: total cholesterol BUN: urea nitrogen GLU: blood sugar level TP: total protein ALb: white protein GLb: globular protein

A. On the 45th day, half of the animals were randomly taken out so as to be female and male each, and blood was collected from the tail vein.
Measure.

B. Rat 90 days of the hypoglycemic agent of the present invention in rats Table 9 shows the effects of the dose on body weight, food consumption, blood routine, biochemical routine, and major organ content coefficient.

[0061]

[Table 9]

(4) Discussion a. According to the results of the blood routine, serum biochemical test and pathological test on days 45 and 90, the test substance, the hypoglycemic agent of the present invention, does not exert any adverse effects on Wistar rats under these experimental concentration conditions.

B. According to the blood glucose levels on days 45 and 90, even if the non-diabetic rats were administered the raw powder of the present invention in large amounts for a long time, none of the cases of hypoglycemia or hyperglycemia were found, whereas the blood glucose of the control group was The price has gone up. The hypoglycemic agent of the present invention is considered to have the effect of adjusting the blood glucose level from both high and low directions to normal blood glucose level.

[0064]

Example 6 Clinical Typical Disease Example 1. A 58-year-old woman was diagnosed with non-insulin-dependent diabetes mellitus. She tried Chinese medicine and new medicines, such as Sojang-gan, healing and dissolving, dominant glucose, and Damicron, but also dieted. Fasting glucose levels varied between 138 and 260 throughout. DAS after 2 years
Start taking tablets containing the raw powder, 20mg per tablet
3 tablets daily, taken before meals, and one week later, the fasting blood glucose level dropped to 180 mg. It became urine sugar (±). After 4 weeks, the fasting blood glucose level is 121 mg.
Became urine sugar (-).

Disease Example 2 Male, 48 years old, diagnosed with insulin-dependent diabetes mellitus, fasting blood glucose level is 310 mg, urine glucose is (++), 200,000 units of insulin are injected daily, blood glucose level is maintained between 110-150 mg, and sometimes low. You have blood sugar or hyperglycemia. One and a half years later, he started taking DAS tablets three times a day. The daily injection of insulin was reduced by 150,000 units. One week later, the fasting blood glucose level was 130 m.
g, urine sugar (±), fasting blood glucose level 115 m after 4 weeks
g, urine sugar is (-). Request to take the tablet No hypoglycemia or hyperglycemia has occurred.

Disease 3 Male, 76 years old, diagnosed with non-insulin dependent diabetes mellitus, fasting blood glucose level 260-300m
g. The fasting blood glucose level was maintained at 135 to 180 by taking eating and drinking treatment with the consumption maru. After 7 years, replace with DAS raw powder tablets, 3 tablets a day, 1 week later, fasting blood glucose level is 140
mg, urine sugar (±). Four weeks later, the fasting blood glucose level is 120 m
g became urine sugar (-).

Disease Example 4. A woman, 51 years old, was determined to have non-insulin-dependent diabetes mellitus, and her fasting blood glucose level was 280 mg and urine glucose was (++). Taking a new drug such as hypoglycemic sugar or damicron, the fasting blood glucose level was 150 to 190 mg and urine glucose (±), but dizziness began to occur after taking the hypoglycemic sugar. That is, it is determined that hypoglycemia has occurred. Then DAS
Three tablets a day were replaced with the powdered tablets, and after one week, the fasting blood glucose level was 145 mg and urine sugar (-). After 4 weeks, the fasting blood glucose level was 107 mg, and the hypoglycemic phenomenon disappeared.

Disease Example 5 A woman, 47 years old, was found to have non-insulin-dependent diabetes mellitus, and had a fasting blood glucose level of 265 mg and urine glucose of (++). Taking Chinese medicines such as doubly-sugar and Damicron and new medicines, the fasting blood glucose level was 120-160 mg and urine glucose (±). Then change to DAS raw powder tablets
After one week, the fasting blood glucose level was 135 mg and urine sugar (±), and after four weeks, the fasting blood glucose level was 105 mg and urine glucose (−).

The blood sugar lowering agent of the present invention may be taken directly from the lotus flower itself, for example, a leaf or stem portion of the flower or a pulverized product thereof. At this time, about 3 to 4 g per day per adult is usually sufficient.

The hypoglycemic agent of the present invention is obtained by extracting the above-mentioned parts of the lotus flower with an extraction solvent and then concentrating the extract to take about 50 to 70 mg per day per adult per day. At this time, the form of the medicine may be various forms such as powder, round, tablet, and pellet, and may be a liquid dissolved in water. You may use together.

[0071]

EFFECTS OF THE INVENTION The hypoglycemic agent of the present invention is not only used for the treatment of diabetes, but also has a sufficient effect when it is added to foods and beverages.

[Brief description of the drawings]

FIG. 1 is a flowchart of an example of a method for extracting an active ingredient from a stone lotus flower.

FIG. 2 is a flowchart of an example of a method for producing a powder (raw powder) from an extract.

FIG. 3 is a flowchart of an example of a method for producing a purified powder (purified powder) from an extract.

FIG. 4 is a flowchart of another example of a method for producing a purified powder.

FIG. 5 is a flowchart of another example of a method for producing a purified powder.

Claims (5)

[Claims] [1] A pure natural blood sugar lowering agent comprising an extract component derived from stone lotus or an extraction solvent thereof. 2. A hypoglycemic agent containing the stone lotus as it is. 3. A hypoglycemic agent containing a concentrate obtained by pulverizing a stone lotus flower and extracting the extract with an extraction solvent. 4. A beverage comprising the ingredient according to claim 1. 5. A food comprising the ingredient according to claim 1.